BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,466,937	A→C	5.1%	T562P (ACT→CCT)	yjgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,466,937	0	A	C	5.1%	1145.4 / 35.4	346	T562P (ACT→CCT)	yjgL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (184/143); new base C (5/14); total (189/157)
Fisher's exact test for biased strand distribution p-value = 1.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 354 total aligned reads displayed.

GGTGAAAAGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGA  >  CP009273/4466790‑4467086

    aaaaGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCt                                                                                                                                                 <  1:3340329/150‑1 (MQ=255)

    aaaaGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCt                                                                                                                                                 >  1:1922678/1‑150 (MQ=255)

       aGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGc                                                                                                                                              <  2:5643623/150‑1 (MQ=255)

         atttatttTTATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCaa                                                                                                                                            <  1:4769596/150‑1 (MQ=255)

          tttatttTGATAATTTAAAATATGATTTAAATGATAATGATAAGTAATCTAATAAAGAAATTTTGAAGAATCAACAAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTTGATTTAGAATGATGCAAACTATCTTGCAAt                                                                                                                                           <  2:2984910/150‑1 (MQ=255)

           ttatttTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTATATTTAGAATTATGCAAACTATATTGCAATc                                                                                                                                          >  2:3895349/1‑150 (MQ=255)

           ttatttTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAACCTATCTTGCAATc                                                                                                                                          <  2:2572769/150‑1 (MQ=255)

           ttatttTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATc                                                                                                                                          >  1:3264155/1‑150 (MQ=255)

             atttTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCga                                                                                                                                        >  1:385984/1‑150 (MQ=255)

             atttTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCga                                                                                                                                        >  1:4442426/1‑150 (MQ=255)

               tttGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCgaga                                                                                                                                      <  1:5367509/150‑1 (MQ=255)

                      aTTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATg                                                                                                                               <  2:633729/150‑1 (MQ=255)

                      aTTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATg                                                                                                                               <  1:581970/150‑1 (MQ=255)

                       tttAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGt                                                                                                                              <  2:3780187/150‑1 (MQ=255)

                       tttAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGt                                                                                                                              >  1:4132300/1‑150 (MQ=255)

                         tAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTAt                                                                                                                            >  2:4988952/1‑150 (MQ=255)

                         tAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGAGTTAGAATGATGCAAACTATCTTGCAATCGAGAGGTAATGTAt                                                                                                                            >  1:5316293/1‑150 (MQ=255)

                          aaaaTATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTAtt                                                                                                                           >  1:1729637/1‑150 (MQ=255)

                           aaaTATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTa                                                                                                                          <  1:4844070/150‑1 (MQ=255)

                            aaTATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTAc                                                                                                                         <  2:3556095/150‑1 (MQ=255)

                             atatGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTAcc                                                                                                                        <  1:2180405/150‑1 (MQ=255)

                               atGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCat                                                                                                                      >  2:4130456/1‑150 (MQ=255)

                                 gATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCatat                                                                                                                    >  1:200693/1‑150 (MQ=255)

                                 gATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGATAATGTATTACCatat                                                                                                                    >  1:3978802/1‑150 (MQ=255)

                                  aTTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCatata                                                                                                                   >  2:4616690/1‑150 (MQ=255)

                                   tttAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCatatat                                                                                                                  >  1:3611809/1‑150 (MQ=255)

                                    ttAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATAtt                                                                                                                 <  2:232309/150‑1 (MQ=255)

                                          gataatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGaa                                                                                                           <  1:5437931/150‑1 (MQ=255)

                                            taatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTCTTACCATATATTAATGAACa                                                                                                         >  2:4000901/1‑150 (MQ=255)

                                            taatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACGTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACa                                                                                                         >  2:1237008/1‑150 (MQ=255)

                                             aatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAACCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAg                                                                                                        <  2:1577477/150‑1 (MQ=255)

                                             aatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAg                                                                                                        <  1:2376521/150‑1 (MQ=255)

                                              atgataaGGAATCTAATAAAGATATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGc                                                                                                       <  1:4532967/150‑1 (MQ=255)

                                                 ataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGtt                                                                                                    <  2:3756779/150‑1 (MQ=255)

                                                   aaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTta                                                                                                  >  1:2937645/1‑150 (MQ=255)

                                                    aGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaa                                                                                                 >  1:3442480/1‑150 (MQ=255)

                                                    aGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaa                                                                                                 >  2:3405606/1‑150 (MQ=255)

                                                    aGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACAATATATTAATGAACAGCGTTtaa                                                                                                 <  1:1085255/150‑1 (MQ=255)

                                                     ggAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaa                                                                                                <  2:1702653/150‑1 (MQ=255)

                                                     ggAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaa                                                                                                <  2:4890386/150‑1 (MQ=255)

                                                      gAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaat                                                                                               >  1:3460368/1‑150 (MQ=255)

                                                       aaTCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaata                                                                                              >  2:4959931/1‑150 (MQ=255)

                                                        aTCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaataa                                                                                             >  2:5267110/1‑150 (MQ=255)

                                                           taataaAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACt                                                                                          <  1:2283749/150‑1 (MQ=255)

                                                             ataaAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTaa                                                                                        >  1:720458/1‑150 (MQ=255)

                                                              taaataaaTTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTaaa                                                                                       <  1:3495533/150‑1 (MQ=255)

                                                                 aGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATcc                                                                                    >  2:4418743/1‑150 (MQ=255)

                                                                 aGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATcc                                                                                    >  2:2096340/1‑150 (MQ=255)

                                                                    aaTTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCAcc                                                                                 <  1:3080382/150‑1 (MQ=255)

                                                                      ttttGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCgg                                                                               <  2:5179586/150‑1 (MQ=255)

                                                                          gaagaaTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGaaaa                                                                           >  1:1004387/1‑150 (MQ=255)

                                                                          gaagaaTCAACCAAATAATGTTATCAAATAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGaaaa                                                                           >  1:3739108/1‑150 (MQ=255)

                                                                           aagaaTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAAt                                                                          >  1:1375044/1‑150 (MQ=255)

                                                                            agaaTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAAtt                                                                         >  2:4597421/1‑150 (MQ=255)

                                                                            agaaTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAAtt                                                                         <  1:4906062/150‑1 (MQ=255)

                                                                            agaaTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAAtt                                                                         >  2:775284/1‑150 (MQ=255)

                                                                                tCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAACCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACg                                                                     <  1:5481609/150‑1 (MQ=255)

                                                                                tCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACg                                                                     >  2:5622705/1‑150 (MQ=255)

                                                                                 cAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGt                                                                    <  2:5434986/150‑1 (MQ=255)

                                                                                  aaCCAGATAATTTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAACCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGta                                                                   <  2:3850517/150‑1 (MQ=255)

                                                                                  aaCCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGta                                                                   >  2:5534504/1‑150 (MQ=255)

                                                                                    ccAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGtata                                                                 >  1:1887031/1‑150 (MQ=255)

                                                                                     cAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAg                                                                <  1:4654369/150‑1 (MQ=255)

                                                                                         tAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAAt                                                            <  1:4317139/150‑1 (MQ=255)

                                                                                         tAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAAt                                                            >  1:3826362/1‑150 (MQ=255)

                                                                                               tATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAaca                                                      >  1:2127460/1‑150 (MQ=255)

                                                                                                aTCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAacac                                                     >  2:671714/1‑150 (MQ=255)

                                                                                                 tCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACt                                                    >  1:1808259/1‑150 (MQ=255)

                                                                                                 tCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACt                                                    >  2:573332/1‑150 (MQ=255)

                                                                                                 tCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACt                                                    <  1:1291386/150‑1 (MQ=255)

                                                                                                  cAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACtt                                                   >  2:1340571/1‑150 (MQ=255)

                                                                                                   aaaGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACttt                                                  <  2:3279190/150‑1 (MQ=255)

                                                                                                    aaGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTg                                                 <  1:2508818/150‑1 (MQ=255)

                                                                                                     aGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTgg                                                >  2:5318479/1‑150 (MQ=255)

                                                                                                        aaaaCTGAATAATGAATACAAACTTAGATTTAGAATGATGCAACCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTg                                             <  2:2380195/150‑1 (MQ=255)

                                                                                                         aaaCTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTgg                                            <  2:1922678/150‑1 (MQ=255)

                                                                                                          aaCTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGga                                           >  2:5660481/1‑150 (MQ=255)

                                                                                                                ataatGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATa                                     <  2:2937645/150‑1 (MQ=255)

                                                                                                                 taatGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAg                                    >  2:500647/1‑150 (MQ=255)

                                                                                                                  aatGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGa                                   >  1:3033602/1‑150 (MQ=255)

                                                                                                                       aTACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTAt                              >  1:2738637/1‑150 (MQ=255)

                                                                                                                          cAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCAc                           <  1:4266233/150‑1 (MQ=255)

                                                                                                                           aaaCTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACt                          >  2:184863/1‑150 (MQ=255)

                                                                                                                             aCTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGc                        >  2:1496508/1‑150 (MQ=255)

                                                                                                                              cTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCa                       >  2:5588852/1‑150 (MQ=255)

                                                                                                                              cTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCa                       >  2:2212755/1‑150 (MQ=255)

                                                                                                                                 aGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATaa                    <  2:943816/150‑1 (MQ=255)

                                                                                                                                    tttAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAaat                 >  2:369846/1‑150 (MQ=255)

                                                                                                                                    tttAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAaat                 <  2:2024567/150‑1 (MQ=255)

                                                                                                                                     ttAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAaata                <  1:127456/150‑1 (MQ=255)

                                                                                                                                       aGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAaataat              <  2:3242439/150‑1 (MQ=255)

                                                                                                                                       aGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAaataat              >  1:5083948/1‑150 (MQ=255)

                                                                                                                                          atgattCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgtg           <  2:2725608/150‑1 (MQ=255)

                                                                                                                                          atgatgCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtgtg           <  1:1680460/150‑1 (MQ=255)

                                                                                                                                              tgCAACCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGc       <  2:4289480/150‑1 (MQ=255)

                                                                                                                                              tgCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGc       >  2:1038909/1‑150 (MQ=255)

                                                                                                                                               gCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGcc      <  1:1612357/150‑1 (MQ=255)

                                                                                                                                               gCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGcc      >  1:1513197/1‑150 (MQ=255)

                                                                                                                                                  aaCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCtgg   >  1:3397346/1‑150 (MQ=255)

                                                                                                                                                   aCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGa  >  1:627562/1‑150 (MQ=255)

GGTGAAAAGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGGA  >  CP009273/4466790‑4467086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`