Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,489,542 A→T 41.5% pseudogene (810/942 nt) yjgX ← pseudogene fragment

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,489,5420AT41.5% 252.6 / inf 361pseudogene (810/942 nt)yjgXpseudogene fragment
Reads supporting (aligned to +/- strand):  ref base A (101/110);  new base T (68/82);  total (169/192)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 363 total aligned reads displayed.

AGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATT  >  CP009273/4489394‑4489691
                                                                                                                                                    |                                                                                                                                                     
 gTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCa                                                                                                                                                     <  1:1779328/150‑1 (MQ=255)
 gTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACa                                                                                                                                                     >  1:505293/1‑150 (MQ=255)
   cTGCCATATATGATTACCTGTGATCAGGAATAAAAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTCAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATCGCGGAAAATGTTCAGTTCaaa                                                                                                                                                   >  2:2510600/1‑150 (MQ=255)
    tGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAAt                                                                                                                                                  >  1:4273668/1‑150 (MQ=255)
     gCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATa                                                                                                                                                 >  1:3843557/1‑150 (MQ=255)
     gCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATa                                                                                                                                                 >  2:3305330/1‑150 (MQ=255)
      ccATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATAc                                                                                                                                                <  1:4223607/150‑1 (MQ=255)
       cATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATAcc                                                                                                                                               <  2:1914602/150‑1 (MQ=255)
       cATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATAcc                                                                                                                                               <  2:3593360/150‑1 (MQ=255)
        atatatGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCg                                                                                                                                              >  2:2617124/1‑150 (MQ=255)
         tatatGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCgg                                                                                                                                             >  1:3296908/1‑150 (MQ=255)
          atatGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGt                                                                                                                                            >  2:529177/1‑150 (MQ=255)
          atatGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGt                                                                                                                                            >  2:333420/1‑150 (MQ=255)
          atatGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGt                                                                                                                                            <  2:142435/150‑1 (MQ=255)
           tatGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTc                                                                                                                                           <  1:401258/150‑1 (MQ=255)
             tGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCAt                                                                                                                                         >  2:4520101/1‑150 (MQ=255)
               aTTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATcc                                                                                                                                       <  1:2529595/150‑1 (MQ=255)
                ttACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCa                                                                                                                                      <  2:4775596/150‑1 (MQ=255)
                    cTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGa                                                                                                                                  <  1:2363892/150‑1 (MQ=255)
                     tgtgATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGaa                                                                                                                                 >  2:1105787/1‑150 (MQ=255)
                      gtgATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGaaa                                                                                                                                >  1:318717/1‑150 (MQ=255)
                        gATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGaaaaa                                                                                                                              <  2:1503617/150‑1 (MQ=255)
                           cAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATAc                                                                                                                           <  2:1381711/150‑1 (MQ=255)
                             ggAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCt                                                                                                                         >  1:653695/1‑150 (MQ=255)
                              gaagaaTAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTg                                                                                                                        <  2:5451694/150‑1 (MQ=255)
                               aagaaTAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGc                                                                                                                       <  1:4120340/150‑1 (MQ=255)
                                 gaaTAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGa                                                                                                                     >  1:862059/1‑150 (MQ=255)
                                  aaTAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGaa                                                                                                                    <  2:2829302/150‑1 (MQ=255)
                                   aTAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGaaa                                                                                                                   <  2:606449/150‑1 (MQ=255)
                                    tAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAAt                                                                                                                  <  2:428359/150‑1 (MQ=255)
                                     aaGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATg                                                                                                                 <  2:76452/150‑1 (MQ=255)
                                        tGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACg                                                                                                              >  1:3404684/1‑150 (MQ=255)
                                           cTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGc                                                                                                           >  2:193927/1‑150 (MQ=255)
                                              aGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGACAAATACCTGCGAAATGACGGGCACt                                                                                                        >  1:3002986/1‑150 (MQ=255)
                                              aGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACt                                                                                                        <  1:3246961/150‑1 (MQ=255)
                                              aGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACt                                                                                                        >  2:4962565/1‑150 (MQ=255)
                                               ggTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTg                                                                                                       >  1:5402323/1‑150 (MQ=255)
                                                  ttCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGAtt                                                                                                    <  1:1419876/150‑1 (MQ=255)
                                                     taaCTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGAttttt                                                                                                 >  2:5395889/2‑150 (MQ=255)
                                                     aaaCTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACTGGCACTGAttttt                                                                                                 >  2:152063/1‑150 (MQ=255)
                                                      aaCTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTc                                                                                                >  2:16836/1‑150 (MQ=255)
                                                      aaCTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTc                                                                                                <  2:4655857/150‑1 (MQ=255)
                                                      aaCTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTc                                                                                                >  1:5241206/1‑150 (MQ=255)
                                                       aCTGTCTGCCGGTATATTACTCTCTCCCTTAATAACCATACCAGTTTAGTTATCTTCGTCTGTCAGCTGATTGAATGTCGGTATATTTTCAGTTCAATTACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCt                                                                                               <  2:706733/150‑1 (MQ=255)
                                                          gTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACt                                                                                            >  1:2767080/1‑150 (MQ=255)
                                                           tCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTg                                                                                           <  2:3244802/150‑1 (MQ=255)
                                                            cTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGa                                                                                          >  1:4828092/1‑150 (MQ=255)
                                                              gCCGGTATAGTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACg                                                                                        <  2:3570666/150‑1 (MQ=255)
                                                                cGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACggg                                                                                      <  2:67471/150‑1 (MQ=255)
                                                                 ggTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGc                                                                                     >  2:4493962/1‑150 (MQ=255)
                                                                 ggTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGc                                                                                     >  1:1877329/1‑150 (MQ=255)
                                                                    atatTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCtga                                                                                  >  2:3036590/1‑150 (MQ=255)
                                                                     tatTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCtgat                                                                                 >  2:4370427/1‑150 (MQ=255)
                                                                     tatTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCtgat                                                                                 >  2:2392446/1‑150 (MQ=255)
                                                                      atTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCtgatg                                                                                >  2:4244003/1‑150 (MQ=255)
                                                                       ttCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCtgatga                                                                               <  1:1378514/150‑1 (MQ=255)
                                                                          ctctctCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGAc                                                                            <  2:3765305/150‑1 (MQ=255)
                                                                           tctctcCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACg                                                                           <  2:4025351/150‑1 (MQ=255)
                                                                            ctctcCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGt                                                                          <  1:5521710/150‑1 (MQ=255)
                                                                            ctctcCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGt                                                                          <  1:2910128/150‑1 (MQ=255)
                                                                             tctcCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGtt                                                                         >  1:1493983/1‑150 (MQ=255)
                                                                              ctcCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTa                                                                        <  2:1469245/150‑1 (MQ=255)
                                                                                  cTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTc                                                                    <  2:4264290/150‑1 (MQ=255)
                                                                                  cTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCAGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTc                                                                    <  1:5366362/150‑1 (MQ=255)
                                                                                      aaaaCCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTg                                                                <  1:4943455/150‑1 (MQ=255)
                                                                                          ccATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCgg                                                            <  2:4462096/150‑1 (MQ=255)
                                                                                          ccATAACAGGTTAGTTATCTACGTCAGTCAGCGGCTTGAATGGCGGTAAATTTTCAGTACAAATACAGGTCATCAAATGAAAAATACCTGCGACATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTgtctg                                                            >  2:3911421/1‑146 (MQ=255)
                                                                                              aaCAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCTAAATGACGGGCACTGATTTTTCTACTGACTGGCTGATGAGACGTTATGTCACTGTCGGtaat                                                        >  1:2958791/1‑150 (MQ=255)
                                                                                               aCAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGATACGTTATGTCACTGGCGGtaata                                                       >  1:4533607/1‑150 (MQ=255)
                                                                                               aCAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGtaata                                                       >  1:1023853/1‑150 (MQ=255)
                                                                                               aCAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGtaata                                                       <  1:3754787/150‑1 (MQ=255)
                                                                                                cAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGtaataa                                                      <  1:4560097/150‑1 (MQ=255)
                                                                                                cAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGtaataa                                                      >  1:1281426/1‑150 (MQ=255)
                                                                                                 aGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTaataat                                                     >  1:1622889/1‑150 (MQ=255)
                                                                                                 aGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTaataat                                                     <  2:547398/150‑1 (MQ=255)
                                                                                                  ggTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATc                                                    <  2:967423/150‑1 (MQ=255)
                                                                                                      agttaTCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGTTAATAATCAGTg                                                >  2:3512430/1‑150 (MQ=255)
                                                                                                        ttaTCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGc                                              >  1:3125160/1‑150 (MQ=255)
                                                                                                         taTCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGca                                             >  1:3965337/1‑150 (MQ=255)
                                                                                                             ttCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACt                                         <  1:4083390/150‑1 (MQ=255)
                                                                                                                gTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTa                                      <  1:2594705/150‑1 (MQ=255)
                                                                                                                  cTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGc                                    >  2:2898710/1‑150 (MQ=255)
                                                                                                                    gTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCt                                  >  2:5577118/1‑150 (MQ=255)
                                                                                                                    gTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTATACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAAAAATCAGTGGCACACTGTAGcca                                  >  2:2769048/1‑149 (MQ=255)
                                                                                                                     tCAGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTc                                 >  2:846198/1‑150 (MQ=255)
                                                                                                                       aGCGGATTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTccc                               >  1:5671928/1‑150 (MQ=255)
                                                                                                                       aGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTccc                               <  1:4144309/150‑1 (MQ=255)
                                                                                                                        gCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCt                              <  1:2201575/150‑1 (MQ=255)
                                                                                                                         cGGATTGAATGGCGGTATATATTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACTTGCTGATGATACTTTATTACAATGGAGGTAATAATCAGTTGAAAACAGTAGCCTACCTg                             >  2:3380111/1‑150 (MQ=255)
                                                                                                                            aTTGAATGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGcac                          >  2:1264687/1‑150 (MQ=255)
                                                                                                                            aTTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGcac                          <  2:505293/150‑1 (MQ=255)
                                                                                                                                  tGGCGGTATATTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGAcc                    <  2:2269545/150‑1 (MQ=255)
                                                                                                                                   ggCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCa                   <  1:471092/150‑1 (MQ=255)
                                                                                                                                          tatTTTCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCGCACTGTAGCCTCCCTGCACATGACCATAatgat            >  1:5580121/1‑150 (MQ=255)
                                                                                                                                           atTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAatgatg           <  1:2016293/150‑1 (MQ=255)
                                                                                                                                             tttCAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGaa         <  1:1124538/150‑1 (MQ=255)
                                                                                                                                                cAGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAg      >  1:4419488/1‑150 (MQ=255)
                                                                                                                                                cAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAg      <  1:3356949/150‑1 (MQ=255)
                                                                                                                                                 aGTTCAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAgg     <  1:3155937/150‑1 (MQ=255)
                                                                                                                                                 aGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAgg     <  2:1581044/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAAATGACGGGCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATT  >  CP009273/4489394‑4489691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑