Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,626,235 G→T 40.4% E138* (GAA→TAA)  creB → response regulator in two‑component regulatory system with CreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,626,2350GT40.4% 298.4 / inf 287E138* (GAA→TAA) creBresponse regulator in two‑component regulatory system with CreC
Reads supporting (aligned to +/- strand):  ref base G (91/80);  new base T (55/61);  total (146/141)
Fisher's exact test for biased strand distribution p-value = 3.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 306 total aligned reads displayed.

TCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCG  >  CP009273/4626086‑4626384
                                                                                                                                                     |                                                                                                                                                     
      gCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTttgaattga                                                                                                                                                 <  2:1123373/150‑1 (MQ=255)
      gCCTGCTTGGGCTGGAAATTGGTGCTGAAGACTACGAGGCTAAACCGTTTTCACCCCGCTAAGTGTGCGCCAGCTTGCGCACCTTACTGCGTCCGGTGAAGAAGTTCACGACGCCGTCTCCCGACATCCGAATTGGAAATTtttaattga                                                                                                                                                 >  1:872411/1‑150 (MQ=255)
          gCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATtgaatga                                                                                                                                             <  2:4752510/150‑1 (MQ=255)
            ttGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAAc                                                                                                                                           >  2:3874400/1‑150 (MQ=255)
            ttGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAAc                                                                                                                                           <  2:2372944/150‑1 (MQ=255)
            ttGGGCTGGAAATAGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAAc                                                                                                                                           <  2:1451843/150‑1 (MQ=255)
                 cTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCcgcg                                                                                                                                      <  2:1939604/150‑1 (MQ=255)
                   ggAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCgcggc                                                                                                                                    <  1:3822626/150‑1 (MQ=255)
                   ggAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCgcggc                                                                                                                                    <  2:3714485/150‑1 (MQ=255)
                   ggAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCgcggc                                                                                                                                    >  1:1717007/1‑150 (MQ=255)
                       aTTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAg                                                                                                                                >  2:3910508/1‑150 (MQ=255)
                          ggTGCTGACGACTACGTGGCTAAACCGTTTTCACACCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATc                                                                                                                             >  2:4911613/1‑150 (MQ=255)
                           gttcTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCa                                                                                                                            <  2:2700022/147‑1 (MQ=255)
                           gTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGAAATTTTGAATTGAAAGAAACCGCGGCGCAGATCa                                                                                                                            >  1:1223143/1‑150 (MQ=255)
                            tGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGTAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAg                                                                                                                           <  2:5041444/150‑1 (MQ=255)
                             gCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGc                                                                                                                          >  1:1971249/1‑150 (MQ=255)
                             gCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGc                                                                                                                          <  2:1399840/150‑1 (MQ=255)
                              cTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCt                                                                                                                         <  2:1136692/150‑1 (MQ=255)
                               tGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTg                                                                                                                        <  1:2242319/150‑1 (MQ=255)
                               tGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTg                                                                                                                        >  2:5008004/1‑150 (MQ=255)
                               tGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTg                                                                                                                        <  2:3584873/150‑1 (MQ=255)
                               tGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTg                                                                                                                        >  2:3063781/1‑150 (MQ=255)
                               tGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTg                                                                                                                        <  2:737222/150‑1 (MQ=255)
                                gacgacgacGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTgg                                                                                                                       <  1:2056981/150‑1 (MQ=255)
                                   gacTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGttt                                                                                                                    <  2:4469239/150‑1 (MQ=255)
                                   gacTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGttt                                                                                                                    <  2:3161635/150‑1 (MQ=255)
                                   gacTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGttt                                                                                                                    >  1:3326454/1‑150 (MQ=255)
                                     cTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAAGTGAATGAACCCGCGGCGCAGATCAGCGGGTTTGa                                                                                                                  >  1:1033323/1‑150 (MQ=255)
                                     cTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGa                                                                                                                  >  2:2123422/1‑150 (MQ=255)
                                           ggCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGcc                                                                                                            <  2:2942650/150‑1 (MQ=255)
                                           ggCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGcc                                                                                                            <  2:4259534/150‑1 (MQ=255)
                                           ggCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGcc                                                                                                            <  1:4959413/150‑1 (MQ=255)
                                             cTAAACCGTTTTCACGCCGCGATGTGTGCGGCAGGGTGCTCACCTTACTGCGTCGGGTGAAGAAGTTCTCGCCGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCAt                                                                                                          <  2:3424612/150‑1 (MQ=255)
                                             cTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCAt                                                                                                          >  1:3605887/1‑150 (MQ=255)
                                              tAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCAtt                                                                                                         >  2:5475449/1‑150 (MQ=255)
                                              tAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCagt                                                                                                         >  2:4036925/1‑148 (MQ=255)
                                              tAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCAtt                                                                                                         >  1:5502360/1‑150 (MQ=255)
                                              tAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCAtt                                                                                                         >  2:4422356/1‑150 (MQ=255)
                                               aaaCCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTa                                                                                                        <  1:3872720/150‑1 (MQ=255)
                                               aaaCCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTa                                                                                                        >  2:2840691/1‑150 (MQ=255)
                                                aaCCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAg                                                                                                       >  2:2032571/1‑150 (MQ=255)
                                                aaCCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAg                                                                                                       >  1:3198737/1‑150 (MQ=255)
                                                     ttttCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTg                                                                                                  <  2:82999/150‑1 (MQ=255)
                                                       ttCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGAc                                                                                                <  1:3561150/150‑1 (MQ=255)
                                                       ttCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGAc                                                                                                <  1:712866/150‑1 (MQ=255)
                                                       ttCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTCGCGCTGAc                                                                                                >  2:1059591/1‑150 (MQ=255)
                                                         cACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTc                                                                                              <  1:832429/150‑1 (MQ=255)
                                                         cACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTc                                                                                              <  1:4877330/150‑1 (MQ=255)
                                                         cACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTc                                                                                              <  1:2863052/150‑1 (MQ=255)
                                                          aCCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCg                                                                                             <  1:3082706/150‑1 (MQ=255)
                                                           ccccGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCgg                                                                                            >  1:4366825/1‑150 (MQ=255)
                                                              cgcgAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTAt                                                                                         <  1:2989605/150‑1 (MQ=255)
                                                              cgcgAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTGTGACACGCCATTAGCGCTGACTCGGtct                                                                                         >  2:3474222/1‑148 (MQ=255)
                                                                cgAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGa                                                                                       >  1:2712877/1‑150 (MQ=255)
                                                                 gAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAg                                                                                      <  1:3987005/150‑1 (MQ=255)
                                                                  aaGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGt                                                                                     >  1:1945171/1‑150 (MQ=255)
                                                                    gtgtGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGttt                                                                                   <  2:1588427/150‑1 (MQ=255)
                                                                     tgtgCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTAGACACGCCATTAGCGCTGACTCGGTATGAGtttt                                                                                  >  1:1060709/1‑150 (MQ=255)
                                                                        gcgcCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTAt                                                                               <  1:5485066/150‑1 (MQ=255)
                                                                          gcCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTAttg                                                                             <  1:5080235/150‑1 (MQ=255)
                                                                             aggctccgcACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGACTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCAATATCTCTGACTCGCTATGAGTATTTCttgttg                                                                          >  2:3367139/7‑150 (MQ=255)
                                                                             aGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTAttgttg                                                                          >  1:1890974/1‑150 (MQ=255)
                                                                                   cgcACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACg                                                                    >  2:233710/1‑150 (MQ=255)
                                                                                    gcACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGt                                                                   <  1:1307731/150‑1 (MQ=255)
                                                                                     cACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGtt                                                                  <  1:5597753/150‑1 (MQ=255)
                                                                                     cACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGtt                                                                  >  1:4590244/1‑150 (MQ=255)
                                                                                      aCCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTa                                                                 >  2:4212067/1‑150 (MQ=255)
                                                                                       ccTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATCTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTAc                                                                >  1:4494685/1‑150 (MQ=255)
                                                                                        cTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACt                                                               >  2:4096442/1‑150 (MQ=255)
                                                                                          tACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCa                                                             >  1:235660/1‑150 (MQ=255)
                                                                                          tACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCa                                                             >  1:4683126/1‑150 (MQ=255)
                                                                                           aCTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTCTTTATTGTTGAAGACGTTACTCaa                                                            >  1:3917975/1‑150 (MQ=255)
                                                                                                 tCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCAcc                                                      <  1:216570/150‑1 (MQ=255)
                                                                                                   gggTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCGGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCgg                                                    >  2:3265373/1‑150 (MQ=255)
                                                                                                      tGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGcc                                                 <  1:5661535/150‑1 (MQ=255)
                                                                                                        aagaagTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCcgc                                               >  1:4288783/1‑150 (MQ=255)
                                                                                                         agaagTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCcgcg                                              >  2:556894/1‑150 (MQ=255)
                                                                                                             gTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTg                                          <  1:3711690/150‑1 (MQ=255)
                                                                                                                ctcGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTc                                       <  2:4254856/150‑1 (MQ=255)
                                                                                                                ctcGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTc                                       <  1:3832521/150‑1 (MQ=255)
                                                                                                                  cGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTccc                                     >  2:1259046/1‑150 (MQ=255)
                                                                                                                   gACGCCGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCg                                    >  1:1506366/1‑150 (MQ=255)
                                                                                                                     cGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGcc                                  <  1:3787381/150‑1 (MQ=255)
                                                                                                                      gCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCa                                 <  2:1226932/150‑1 (MQ=255)
                                                                                                                       ccGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAg                                <  1:1363506/150‑1 (MQ=255)
                                                                                                                        cttctcCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGc                               >  1:1178685/3‑150 (MQ=255)
                                                                                                                        cGTCTCCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGc                               >  1:4842347/1‑150 (MQ=255)
                                                                                                                        cGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGc                               >  1:4072853/1‑150 (MQ=255)
                                                                                                                           ctcCCGTCATCCGTATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAAc                            >  2:3770907/1‑150 (MQ=255)
                                                                                                                            tcCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACt                           >  1:591567/1‑150 (MQ=255)
                                                                                                                               cGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGAt                        <  2:1890974/150‑1 (MQ=255)
                                                                                                                                 tCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATgg                      <  1:2606929/150‑1 (MQ=255)
                                                                                                                                   aTCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGAt                    >  2:3636375/1‑150 (MQ=255)
                                                                                                                                      cGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGc                 >  2:657698/1‑150 (MQ=255)
                                                                                                                                        tATTGGACATTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGt               >  1:917817/1‑150 (MQ=255)
                                                                                                                                             gACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATggg          <  1:4223302/150‑1 (MQ=255)
                                                                                                                                                aTTTTTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAg       >  2:1442784/1‑150 (MQ=255)
                                                                                                                                                 ttttTAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGa      <  1:602509/150‑1 (MQ=255)
                                                                                                                                                    tgaattgaatGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATgc   <  1:5432549/150‑1 (MQ=255)
                                                                                                                                                     gaattgaatGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATgcg  >  1:949178/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCG  >  CP009273/4626086‑4626384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑