Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 419,663 G→T 39.7% E564* (GAA→TAA)  malZ → maltodextrin glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273419,6630GT39.7% 369.9 / inf 306E564* (GAA→TAA) malZmaltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base G (87/97);  new base T (51/71);  total (138/168)
Fisher's exact test for biased strand distribution p-value = 3.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

ACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACG  >  CP009273/419514‑419812
                                                                                                                                                     |                                                                                                                                                     
    gCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAgt                                                                                                                                                   >  2:2199738/1‑150 (MQ=255)
     cGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAgtg                                                                                                                                                  <  1:126989/150‑1 (MQ=255)
      gAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAgtgg                                                                                                                                                 >  2:5576753/1‑150 (MQ=255)
       aaTGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAgtggt                                                                                                                                                <  2:1950897/150‑1 (MQ=255)
        aTGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAgtggtg                                                                                                                                               >  1:3153552/1‑150 (MQ=255)
         tGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGc                                                                                                                                              >  1:5033665/1‑150 (MQ=255)
          gATTTCGCTGCGTAAGTAATGTCATGAGGTACGTCATGGCGGCTGTCATGTTCTTTATGCTTAAGATTACGTGGTGGTATTTGTCCGCGTGCTGTATCAGCAACGTGTACTGGTGGCAATCATGCGTGGCGTTGCGTGTGAAgtggtgat                                                                                                                                             <  2:3634318/150‑3 (MQ=255)
          gATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCTGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGACTCAGCAACGTGTACTGGTGGCAATCTACCGTGGCGAGGCCTGTTAAGTGGTGcc                                                                                                                                             <  2:3198542/150‑2 (MQ=255)
          gATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGAGAACGTGGTGGTATTGGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCt                                                                                                                                             <  2:1041938/150‑1 (MQ=255)
             tGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTAcc                                                                                                                                          <  1:116966/150‑1 (MQ=255)
              gcgcTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTAccc                                                                                                                                         <  1:526901/150‑1 (MQ=255)
               cgcTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCcg                                                                                                                                        >  2:2630746/1‑150 (MQ=255)
                      tAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCAcc                                                                                                                                 <  1:1490685/150‑1 (MQ=255)
                      tAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCAcc                                                                                                                                 >  2:2155608/1‑150 (MQ=255)
                         gAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGtt                                                                                                                              >  2:776445/1‑150 (MQ=255)
                         gAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGtt                                                                                                                              <  1:2697035/150‑1 (MQ=255)
                          aaaaGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGttt                                                                                                                             <  2:2489716/150‑1 (MQ=255)
                          aaaaGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGttt                                                                                                                             <  2:3740160/150‑1 (MQ=255)
                          aaaaGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGttt                                                                                                                             <  2:1118422/150‑1 (MQ=255)
                            aaGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTtct                                                                                                                           <  2:3014168/150‑1 (MQ=255)
                             aGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTtctc                                                                                                                          <  2:226522/150‑1 (MQ=255)
                                cAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAAt                                                                                                                       >  1:4885104/1‑150 (MQ=255)
                                      cTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTg                                                                                                                 >  1:876021/1‑150 (MQ=255)
                                       tACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgc                                                                                                                >  2:3893585/1‑150 (MQ=255)
                                        aCGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgca                                                                                                               >  1:1920291/1‑150 (MQ=255)
                                         cGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaa                                                                                                              >  1:445488/1‑150 (MQ=255)
                                          gTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGTTAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaat                                                                                                             >  2:68514/1‑150 (MQ=255)
                                             aTGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATTAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatggc                                                                                                          >  2:2032319/1‑150 (MQ=255)
                                             aTGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatggc                                                                                                          <  1:1131453/150‑1 (MQ=255)
                                               ggcggcTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatggcaa                                                                                                        >  1:5452769/1‑150 (MQ=255)
                                               ggcggcTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatggcaa                                                                                                        <  2:258032/150‑1 (MQ=255)
                                                gcggcTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatggcaat                                                                                                       <  2:1862363/150‑1 (MQ=255)
                                                gcggcTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatggcaat                                                                                                       <  2:82926/150‑1 (MQ=255)
                                                 cggcTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatggcaatg                                                                                                      <  1:1417329/150‑1 (MQ=255)
                                                 cggcTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatggcaatg                                                                                                      <  1:613100/150‑1 (MQ=255)
                                                     tGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCaaa                                                                                                  <  2:3686881/150‑1 (MQ=255)
                                                      gTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaag                                                                                                 <  2:2745067/150‑1 (MQ=255)
                                                       tCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaaga                                                                                                >  2:3259205/1‑150 (MQ=255)
                                                       tCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaaga                                                                                                >  2:3290318/1‑150 (MQ=255)
                                                       tCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaaga                                                                                                >  2:1749374/1‑150 (MQ=255)
                                                       tCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaaga                                                                                                >  1:3868380/1‑150 (MQ=255)
                                                         aGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaagaag                                                                                              <  2:4470205/150‑1 (MQ=255)
                                                         aGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaagaag                                                                                              <  2:1637103/150‑1 (MQ=255)
                                                         aGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaagaag                                                                                              <  1:4965668/150‑1 (MQ=255)
                                                         aGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaagaag                                                                                              <  1:829843/150‑1 (MQ=255)
                                                          ggTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAgg                                                                                             <  1:1333857/150‑1 (MQ=255)
                                                            tGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGc                                                                                           >  1:3017173/1‑150 (MQ=255)
                                                            tGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGc                                                                                           >  2:3533512/1‑150 (MQ=255)
                                                             gCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCa                                                                                          >  1:5357129/1‑150 (MQ=255)
                                                                gTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATgg                                                                                       >  2:2318428/1‑150 (MQ=255)
                                                                  aTGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGc                                                                                     >  2:2888646/1‑150 (MQ=255)
                                                                    gCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCaa                                                                                   <  1:982015/150‑1 (MQ=255)
                                                                     cGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAac                                                                                  >  2:14599/1‑150 (MQ=255)
                                                                     cGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAac                                                                                  <  1:2905711/150‑1 (MQ=255)
                                                                       gAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAactg                                                                                <  2:15645/150‑1 (MQ=255)
                                                                       gAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAactg                                                                                <  1:512678/150‑1 (MQ=255)
                                                                       gAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAactg                                                                                <  2:1728667/150‑1 (MQ=255)
                                                                          gATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAactgact                                                                             >  2:3439082/1‑150 (MQ=255)
                                                                             aaCGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACtgactgac                                                                          <  1:4807823/150‑1 (MQ=255)
                                                                                 tggtggTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGa                                                                      >  1:3439586/1‑150 (MQ=255)
                                                                                   gtggtATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGAtt                                                                    <  1:5478330/150‑1 (MQ=255)
                                                                                        aTTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGc                                                               >  1:3324546/1‑150 (MQ=255)
                                                                                        aTTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGc                                                               <  1:4715170/150‑1 (MQ=255)
                                                                                         tttGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCt                                                              >  2:1432076/1‑150 (MQ=255)
                                                                                          ttGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCtt                                                             <  1:238186/150‑1 (MQ=255)
                                                                                           tGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCttt                                                            <  2:90100/150‑1 (MQ=255)
                                                                                               cgcgTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTtgcct                                                        >  1:2551368/1‑150 (MQ=255)
                                                                                                 cgTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTtgcctgc                                                      >  2:4266877/1‑150 (MQ=255)
                                                                                                   tGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCa                                                    <  1:4204384/150‑1 (MQ=255)
                                                                                                   tGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCa                                                    <  1:1053219/150‑1 (MQ=255)
                                                                                                      tGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCAttt                                                 >  2:132220/1‑150 (MQ=255)
                                                                                                       gAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTc                                                <  2:4942701/150‑1 (MQ=255)
                                                                                                       gAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTc                                                >  1:2005656/1‑150 (MQ=255)
                                                                                                       gAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTc                                                <  1:4289486/150‑1 (MQ=255)
                                                                                                           cAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCt                                            >  1:5439633/1‑150 (MQ=255)
                                                                                                            aGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTa                                           <  1:2630768/150‑1 (MQ=255)
                                                                                                            aGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTa                                           <  1:2630136/150‑1 (MQ=255)
                                                                                                             gCACCGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTAc                                          <  1:3985671/150‑1 (MQ=255)
                                                                                                              cAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACg                                         <  1:2155608/150‑1 (MQ=255)
                                                                                                                aCGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGt                                       <  2:2079804/150‑1 (MQ=255)
                                                                                                                 cGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTa                                      <  1:5566639/150‑1 (MQ=255)
                                                                                                                 cGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTa                                      <  1:1575998/150‑1 (MQ=255)
                                                                                                                     tACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGa                                  <  2:4041865/150‑1 (MQ=255)
                                                                                                                     tACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGa                                  <  2:2397368/150‑1 (MQ=255)
                                                                                                                      aCTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGAt                                 <  1:354464/150‑1 (MQ=255)
                                                                                                                       cTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATg                                <  1:3607183/150‑1 (MQ=255)
                                                                                                                          gtggCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAAc                             >  2:5679913/1‑150 (MQ=255)
                                                                                                                            ggCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTa                           >  1:2077961/1‑150 (MQ=255)
                                                                                                                               aaTCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACg                        >  1:1750691/1‑150 (MQ=255)
                                                                                                                                    aCCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTta                   <  1:896027/150‑1 (MQ=255)
                                                                                                                                     ccGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTtat                  <  1:2221003/150‑1 (MQ=255)
                                                                                                                                      cGTGGCGTTGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCTTGCTATGGCATTGCATAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTtata                 <  2:5408832/150‑1 (MQ=255)
                                                                                                                                       gTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATaa                <  1:2860345/150‑1 (MQ=255)
                                                                                                                                         ggCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACg              >  2:2244423/1‑150 (MQ=255)
                                                                                                                                            gAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCt           >  1:2787164/1‑150 (MQ=255)
                                                                                                                                             aGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCtt          <  1:3645093/150‑1 (MQ=255)
                                                                                                                                             aGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCtt          <  2:3902729/150‑1 (MQ=255)
                                                                                                                                                 cTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGt      <  2:4436563/150‑1 (MQ=255)
                                                                                                                                                   gtgAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGa    >  2:388333/1‑150 (MQ=255)
                                                                                                                                                   gtgAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGa    <  2:3478477/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACCAGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGACG  >  CP009273/419514‑419812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑