Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 596,756 G→T 16.0% E863* (GAG→TAG)  cusA → copper/silver efflux system, membrane component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273596,7560GT16.0% 1113.9 / 141.2 371E863* (GAG→TAG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base G (164/146);  new base T (29/32);  total (193/178)
Fisher's exact test for biased strand distribution p-value = 4.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 375 total aligned reads displayed.

TGAAAACCGAGAATGCGCGCCCGACGAGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATC  >  CP009273/596607‑596905
                                                                                                                                                     |                                                                                                                                                     
 gAAAACCGAGAATGCGCGCCCGACGAGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGa                                                                                                                                                      >  1:4993915/1‑150 (MQ=255)
   aaaCCGAGAATGCGCGCCCGACGAGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGc                                                                                                                                                    >  1:2801960/1‑150 (MQ=255)
    aaCCGATAATGCGCGCCCGACGAGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGGTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCt                                                                                                                                                   >  2:1723443/1‑150 (MQ=255)
         agaATGCGCGCCCGACGAGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTgg                                                                                                                                              <  2:505748/150‑1 (MQ=255)
          gaATGCGCGCCCGACGAGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGa                                                                                                                                             >  1:1838821/1‑150 (MQ=255)
            aTGCGCGCCCGACGAGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAgc                                                                                                                                           >  2:5605390/1‑150 (MQ=255)
             tGCGCGCCCGACGAGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAgcg                                                                                                                                          <  2:5334640/150‑1 (MQ=255)
                gcgcCCGACGAGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCTAGCTACTGGAgcgcgc                                                                                                                                       >  1:2209315/1‑150 (MQ=255)
                 cgcCCGACGAGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGTAGCGCGcc                                                                                                                                      >  2:4655846/1‑150 (MQ=255)
                  gcCCGACGAGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCa                                                                                                                                     >  2:1229881/1‑150 (MQ=255)
                    ccGACGAGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAAc                                                                                                                                   >  1:290695/1‑150 (MQ=255)
                    ccGACGAGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAAc                                                                                                                                   <  2:4821365/150‑1 (MQ=255)
                      gacgaGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCa                                                                                                                                 >  1:4297146/1‑150 (MQ=255)
                       acgaGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCTAGCTACTGGAGCGCGCCAACCAt                                                                                                                                <  2:899879/150‑1 (MQ=255)
                         gaGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCAGTCTCCGGGCAGTTCGAGCTACTGGAGAGCGCCAACCATaa                                                                                                                              >  2:2227163/1‑150 (MQ=255)
                          aGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAg                                                                                                                             <  2:3067621/150‑1 (MQ=255)
                           gCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGc                                                                                                                            <  2:4410695/150‑1 (MQ=255)
                                aTTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCTAGCTACTGGAGCGCGCCAACCATAAGCTTaaa                                                                                                                       <  1:985383/150‑1 (MQ=255)
                                  ttATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACt                                                                                                                     <  2:1973773/150‑1 (MQ=255)
                                  ttATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACt                                                                                                                     <  2:2006019/150‑1 (MQ=255)
                                   tataTCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTc                                                                                                                    <  2:1805482/150‑1 (MQ=255)
                                    atatCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCTAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCa                                                                                                                   <  2:2987423/150‑1 (MQ=255)
                                    atatCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCa                                                                                                                   <  1:774737/150‑1 (MQ=255)
                                    agatCGAGGCCCGCTCTCGGGACATCGAGTCGGAGGTTCACGATTTGCAAAAAGCGAGAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCTAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCa                                                                                                                   <  2:2987565/148‑1 (MQ=255)
                                      atCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATg                                                                                                                 >  1:105096/1‑150 (MQ=255)
                                        cGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCCTGGt                                                                                                               >  2:5498878/1‑150 (MQ=255)
                                              ccGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGAt                                                                                                         <  1:2992385/150‑1 (MQ=255)
                                              ccGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGAt                                                                                                         <  1:155965/150‑1 (MQ=255)
                                                 cgATCGTGACATGGTGTCGGTGGTTCACGATTTGCAATAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGAc                                                                                                      <  2:4532201/150‑1 (MQ=255)
                                                 cgATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAACACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGAc                                                                                                      <  2:2519245/150‑1 (MQ=255)
                                                    tCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCTAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGtt                                                                                                   >  1:5611897/1‑150 (MQ=255)
                                                     cGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTtg                                                                                                  <  1:4652210/150‑1 (MQ=255)
                                                      gTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTtga                                                                                                 >  1:1069097/1‑150 (MQ=255)
                                                      gTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCCCCGGGCAGTTCGGGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTtga                                                                                                 >  2:2836223/1‑150 (MQ=255)
                                                       tGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCTAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTtgat                                                                                                <  1:2399665/150‑1 (MQ=255)
                                                       tGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTtgat                                                                                                >  2:3273727/1‑150 (MQ=255)
                                                       tGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTtgat                                                                                                <  2:4050710/150‑1 (MQ=255)
                                                         aCATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAATCCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTtgatga                                                                                              >  2:1060396/1‑150 (MQ=255)
                                                           aTGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGAtt                                                                                            <  1:1363802/150‑1 (MQ=255)
                                                           aTGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGAtt                                                                                            <  2:5041434/150‑1 (MQ=255)
                                                            tGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCTAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTa                                                                                           <  2:2318201/150‑1 (MQ=255)
                                                              gtgtCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATc                                                                                         <  2:4652234/150‑1 (MQ=255)
                                                              gtgtCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATc                                                                                         >  2:4784822/1‑150 (MQ=255)
                                                                gtCGGTGGTTCACGATTTGCAAAAATCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCtt                                                                                       >  2:4346966/1‑150 (MQ=255)
                                                                gtCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCtt                                                                                       >  1:2480971/1‑150 (MQ=255)
                                                                 acGGTGGATCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCACTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCAAGGTGCCCATGACGTTGATGAATATCtaa                                                                                      >  2:3922140/2‑148 (MQ=255)
                                                                  cGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCg                                                                                     <  1:1804988/150‑1 (MQ=255)
                                                                    gtggtTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTg                                                                                   >  1:3072807/1‑150 (MQ=255)
                                                                      ggtTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCt                                                                                 <  2:376287/150‑1 (MQ=255)
                                                                        ttCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCtgt                                                                               >  2:83980/1‑150 (MQ=255)
                                                                           aCGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCtgttgt                                                                            >  1:5166526/1‑150 (MQ=255)
                                                                            cGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTa                                                                           <  1:1228090/150‑1 (MQ=255)
                                                                            cGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTa                                                                           <  1:2112051/150‑1 (MQ=255)
                                                                             gATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTCTCTTCGTGCTGTTGTAt                                                                          >  2:4963031/1‑150 (MQ=255)
                                                                             gATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTAt                                                                          >  2:4962703/1‑150 (MQ=255)
                                                                              aTTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATc                                                                         >  1:1328397/1‑150 (MQ=255)
                                                                                   cAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCg                                                                    >  1:3488705/1‑150 (MQ=255)
                                                                                   cAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTAATGGAGCGCGCCAACCATAAGCTTAAACTCATTGTGCCGATGAAGTTGATGATTATCTTCGTGCTGTTGTATCTGGCg                                                                    <  2:3399034/150‑1 (MQ=255)
                                                                                    aaaaaGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGt                                                                   <  2:610780/150‑1 (MQ=255)
                                                                                    aaaaaGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGt                                                                   >  2:22765/1‑150 (MQ=255)
                                                                                    aaaaaGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGt                                                                   <  2:755669/150‑1 (MQ=255)
                                                                                             tAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCTAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCggg                                                          >  1:5183535/1‑150 (MQ=255)
                                                                                             tAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCggg                                                          >  2:2628555/1‑150 (MQ=255)
                                                                                             tAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCggg                                                          >  2:764690/1‑150 (MQ=255)
                                                                                                  gAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCTAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGc                                                     >  1:4878623/1‑150 (MQ=255)
                                                                                                    aaaaGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGa                                                   >  1:1144364/1‑150 (MQ=255)
                                                                                                           cAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCTAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTg                                            >  1:4606698/1‑150 (MQ=255)
                                                                                                           cAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCTAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTg                                            >  2:4092083/1‑150 (MQ=255)
                                                                                                            aGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGGTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGc                                           >  2:2676628/1‑150 (MQ=255)
                                                                                                             gTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTACACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCt                                          <  1:4133420/150‑1 (MQ=255)
                                                                                                             gTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCt                                          >  2:3649155/1‑150 (MQ=255)
                                                                                                             gTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCt                                          <  1:1413910/150‑1 (MQ=255)
                                                                                                               tAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGa                                        <  1:5317725/150‑1 (MQ=255)
                                                                                                                  aaCCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCTAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTa                                     >  1:4428083/1‑150 (MQ=255)
                                                                                                                  aaCCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTa                                     >  1:5489373/1‑150 (MQ=255)
                                                                                                                  aaCCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTa                                     >  2:4292615/1‑150 (MQ=255)
                                                                                                                  aaCCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCGGTTGTATCTGGCGTTCCGTCGGGTGGGCGACGCGTTGCTGATTa                                     >  2:1319289/1‑150 (MQ=255)
                                                                                                                   aCCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTAt                                    <  1:2066432/150‑1 (MQ=255)
                                                                                                                   aCCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTAt                                    >  2:334963/1‑150 (MQ=255)
                                                                                                                   aCCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTAt                                    >  1:3636179/1‑150 (MQ=255)
                                                                                                                    ccGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATc                                   >  1:171531/1‑150 (MQ=255)
                                                                                                                      gggCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATcag                                 >  1:2000812/1‑150 (MQ=255)
                                                                                                                          aCCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCagcagc                             >  2:316998/1‑150 (MQ=255)
                                                                                                                           ccAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCg                            >  2:1882838/1‑150 (MQ=255)
                                                                                                                           ccAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCg                            <  1:2628555/150‑1 (MQ=255)
                                                                                                                           acAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCg                            <  2:4732426/149‑1 (MQ=255)
                                                                                                                             aGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTa                          >  2:554739/1‑150 (MQ=255)
                                                                                                                             aGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTAGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTa                          >  2:362755/1‑150 (MQ=255)
                                                                                                                               cGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTAcc                        <  1:908562/150‑1 (MQ=255)
                                                                                                                               cGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTAcc                        <  1:324701/150‑1 (MQ=255)
                                                                                                                               cGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTAcc                        <  1:381731/150‑1 (MQ=255)
                                                                                                                                   gCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGttt                    >  1:3437494/1‑150 (MQ=255)
                                                                                                                                    cATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTg                   >  1:3474370/1‑150 (MQ=255)
                                                                                                                                     aTTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTgc                  >  2:2916051/1‑150 (MQ=255)
                                                                                                                                         tcCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCtg              >  1:3767658/1‑150 (MQ=255)
                                                                                                                                             ggCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACGCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCtggtgg          <  1:3607458/150‑1 (MQ=255)
                                                                                                                                               cAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTCCCGTTTGCGCTGGTGggc        >  1:5276206/1‑150 (MQ=255)
                                                                                                                                               cAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGggc        >  2:2735446/1‑150 (MQ=255)
                                                                                                                                                aGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGggcg       <  1:3911737/150‑1 (MQ=255)
                                                                                                                                                    cGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCAt   >  2:13904/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGAAAACCGAGAATGCGCGCCCGACGAGCTGGATTTATATCGATGCCCGCGATCGTGACATGGTGTCGGTGGTTCACGATTTGCAAAAAGCGATAGCTGAAAAAGTGCAGTTAAAACCGGGCACCAGCGTGGCATTCTCCGGGCAGTTCGAGCTACTGGAGCGCGCCAACCATAAGCTTAAACTCATGGTGCCGATGACGTTGATGATTATCTTCGTGCTGTTGTATCTGGCGTTCCGTCGGGTGGGCGAAGCGTTGCTGATTATCAGCAGCGTACCGTTTGCGCTGGTGGGCGGCATC  >  CP009273/596607‑596905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑