Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 816,078 C→G 7.0% intergenic (+34/‑171) ybhL → / → ybhM UPF0005 family inner membrane protein/UPF0005 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273816,0780CG 7.0% 784.1 / 3.7 255intergenic (+34/‑171)ybhL/ybhMUPF0005 family inner membrane protein/UPF0005 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (101/132);  new base G (3/19);  total (104/151)
Fisher's exact test for biased strand distribution p-value = 6.18e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

TACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATT  >  CP009273/815930‑816226
                                                                                                                                                    |                                                                                                                                                    
tACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTc                                                                                                                                                      >  2:4218369/1‑149 (MQ=255)
tACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTc                                                                                                                                                      >  2:3637682/1‑149 (MQ=255)
tACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTc                                                                                                                                                      >  1:1357657/1‑149 (MQ=255)
 aCCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCg                                                                                                                                                     <  1:165761/149‑1 (MQ=255)
   ccGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCggg                                                                                                                                                   >  2:1855591/1‑149 (MQ=255)
    cgcgACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGa                                                                                                                                                  <  1:3622399/149‑1 (MQ=255)
        acacGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGAtttt                                                                                                                                              <  1:4523869/149‑1 (MQ=255)
        acacGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGAtttt                                                                                                                                              <  2:3361876/149‑1 (MQ=255)
        acacGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGAtttt                                                                                                                                              <  1:3849692/149‑1 (MQ=255)
          acGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGAtttttt                                                                                                                                            <  1:146479/149‑1 (MQ=255)
           cGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGAttttttt                                                                                                                                           >  2:1411207/1‑149 (MQ=255)
               gAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTa                                                                                                                                       <  2:1157819/149‑1 (MQ=255)
               gAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTa                                                                                                                                       <  2:3650579/149‑1 (MQ=255)
               gAACCTGCGCAAATAGTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTa                                                                                                                                       <  2:618330/149‑1 (MQ=255)
                 aCCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACt                                                                                                                                     >  1:3721202/1‑149 (MQ=255)
                    tGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGCTTTTTTTCTTAATaaa                                                                                                                                  >  2:4299049/1‑149 (MQ=255)
                     gcgcAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTaaaa                                                                                                                                 <  1:4252160/149‑1 (MQ=255)
                     gcgcAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTaaaa                                                                                                                                 >  2:909316/1‑149 (MQ=255)
                      cgcAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTaaaaa                                                                                                                                <  1:2620858/149‑1 (MQ=255)
                      cgcAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTaaaaa                                                                                                                                <  1:1538741/149‑1 (MQ=255)
                       gcAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTACACCAGCCAAAACCCGGCCATGTGACGGTTTTTTTTGATTAATAAAAAc                                                                                                                               >  2:897226/1‑149 (MQ=255)
                          aaTATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGac                                                                                                                            <  2:649129/149‑1 (MQ=255)
                          aaTATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGac                                                                                                                            >  1:4203933/1‑149 (MQ=255)
                           atatTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGaca                                                                                                                           <  2:3065596/149‑1 (MQ=255)
                              ttCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACtt                                                                                                                        >  1:850022/1‑149 (MQ=255)
                               tCCTTTTTTGGCGGTTTAACTTTTTATCTGGACTTCATCAACCTGTTCCTGTTTTTTTTGGGTATATTCGGAAACCGCGGTTAATTATTTTTCACCTTCACTATCCCGGCATTGTGTGGGGATTTTTTTCTTAATAAAAACGACACTTa                                                                                                                       <  2:2001174/149‑1 (MQ=255)
                                ccATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACAAACCCCGCCATGTGTCGGGATTTTTTTATTAATAAAAACGACACATaa                                                                                                                      >  1:1760268/1‑149 (MQ=255)
                                 cATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCAGGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAAc                                                                                                                     <  2:4268794/149‑1 (MQ=255)
                                     cTTGGCGCTTTAACCTTTTATCTGGACTTAATCAACCTGTTCCTTTTGTTGTTGCTTTTTTTCGGCAACCGCCGTTAATTATTTTTCACCATCACTATCCCGGCCTTGTGTGGTTATTTTTTTATTACTAAAAACGACACTTAACAAGt                                                                                                                 <  1:4074263/149‑1 (MQ=255)
                                       tGGCGCTTTAACCTTGTTTCTGGACTTCTTAAACCTTTTCCTTATTTTGTTGCGTATCTTCGGAAACCGCCGTTAATTCTTTTTCACAATCACTATCCCGGCCATGTGTGGGTATTTTTTTCTTCATAAAAACGACACTTAACAAGTAc                                                                                                               <  1:3479485/149‑1 (MQ=255)
                                       tGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGTTGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCTTGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTaa                                                                                                               <  2:620163/149‑2 (MQ=255)
                                           gctttAACATTTTTTCTGTACTTCATAAACGTGTTCTTGATGTTGTTGGGTATCTTCGGAAACCGCCTTTAATTATTTTTCACAATCAATAGCCCGGCCTTGTGTCGGTATTTTTTTCTTACTAAACACGACACTTAACAAGTACCAgg                                                                                                           <  1:4472790/146‑1 (MQ=255)
                                            cgTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCAGGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGt                                                                                                          <  2:2643234/149‑1 (MQ=255)
                                              ttAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTAACCCTCACTATCCCGGCCATTTGTGGGGAATTTTTTCTTACTAAAAACGACACTTAAAAAGTACCAGGtat                                                                                                        >  2:1531890/1‑149 (MQ=255)
                                               tAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGtata                                                                                                       >  2:639331/1‑149 (MQ=255)
                                                  ccTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATaaaa                                                                                                    >  2:1746187/1‑149 (MQ=255)
                                                   cTTTTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGTATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCTTGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAat                                                                                                   <  2:251520/149‑1 (MQ=255)
                                                   cTTGTTTCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATATTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCAATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAat                                                                                                   <  2:3503284/149‑1 (MQ=255)
                                                   cTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAat                                                                                                   >  2:4615018/1‑149 (MQ=255)
                                                    ttGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAata                                                                                                  >  1:752040/1‑149 (MQ=255)
                                                      gTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATg                                                                                                <  2:358500/149‑1 (MQ=255)
                                                        aTCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTATTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGcc                                                                                              >  1:2222613/1‑149 (MQ=255)
                                                         tCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCt                                                                                             >  2:1652453/1‑149 (MQ=255)
                                                         tCTGGACTTCAACAACCTGTTCCTGTTGTTGTTGCGTATCTTCGGCAACCGCCGTTAATTCTTTTACACCATCACTATCCCGGCCTTGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCt                                                                                             <  1:1739679/149‑1 (MQ=255)
                                                          cTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAAAGACACTTAACAATTACCAGGTATAAAATATGCCtt                                                                                            >  1:521203/1‑149 (MQ=255)
                                                          cTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAAAGACACTTAACAAGTACCAGGTATAAAATATGCCtt                                                                                            >  1:3868528/1‑149 (MQ=255)
                                                           tGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAAAGACACTTAAAAAGTACCAGGTATAAAATATGCCTTa                                                                                           >  2:1214304/1‑149 (MQ=255)
                                                                 tcatcaACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACg                                                                                     >  2:2245395/1‑149 (MQ=255)
                                                                    tcaACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCAGGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGc                                                                                  <  2:1045049/149‑1 (MQ=255)
                                                                        ccTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTATTTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGaa                                                                              <  2:2206280/149‑1 (MQ=255)
                                                                        ccTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGaa                                                                              >  1:2329280/1‑149 (MQ=255)
                                                                         cTGTTCCTGTTGTTTTTGCGGATATTGGGCAACCGCCGTTAATTCTTTTTCACCAACACTTGCCGGGCCATGTGTGGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAg                                                                             <  1:4152150/149‑1 (MQ=255)
                                                                           gTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCg                                                                           <  2:1790516/149‑1 (MQ=255)
                                                                            ttCCTTATGTTGTTGCGTATCTTCGGCAACCGCCTTTAATTCTTTTTCACCATCACTATCCCGGCCTTGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGt                                                                          <  1:3414787/149‑1 (MQ=255)
                                                                            ttCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGt                                                                          <  2:2840232/149‑1 (MQ=255)
                                                                            ttCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGt                                                                          >  1:1510371/1‑149 (MQ=255)
                                                                             tCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGTCGAGCGGAAGCGtt                                                                         >  2:3954361/1‑149 (MQ=255)
                                                                             tCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGtt                                                                         >  2:3953883/1‑149 (MQ=255)
                                                                              ccTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTATTTTTCACCATCACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGttt                                                                        <  1:295348/149‑1 (MQ=255)
                                                                                 gATGTTGTTGCGTATCTTCGGAAACCGCCGTTAATTCTTTTTCACCATCAATATCCCGGCAATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCt                                                                     <  1:1878957/149‑1 (MQ=255)
                                                                                 gATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTAACCAACACTATACCGGCCAAGTGTCGGGGTTTTTTTATTACTAAAAAAGACACATAACAAATACCAGGTATAAAATATTCCTTATTGAAGACCGGAAGCGttttgg                                                                     >  1:1595042/1‑147 (MQ=255)
                                                                                      tgttgCGTATCTTCGGCAACCGCCGTTAATTCTTTTCCACCATCACTAGCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATc                                                                <  1:1115732/149‑1 (MQ=255)
                                                                                      tgttgCGGATCTTCGGCAACCGCCTTTAATTCTTTTTCACCATCACTAGCCCGGCCTTGTGTCGGTTTTTTTTTCTTACTAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATc                                                                <  1:624176/149‑1 (MQ=255)
                                                                                       gttgCGTATCTTCGGAACCCGCCTTTAATACTTTTACACCATCACTAGCCCGCCCATGTGTCGGGATTTTTTTCTTAATAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATcc                                                               <  1:951935/149‑1 (MQ=255)
                                                                                        ttgCGGATCTTCGGCAACCGCCGTTATTTCTTTTTCACCATCACTATCCCGGCCTTGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCa                                                              <  1:689551/149‑1 (MQ=255)
                                                                                        ttgCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCa                                                              >  2:540330/1‑149 (MQ=255)
                                                                                             gATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACAATCCCGGCCATGTGTCGGGATTTTTTTATTACAAAAAAAGACACATAACAAGTACCAGGTATAAAATATGCCTTATTGACGAGCGGAAGCGTTTTCTGTATACAAAAAt                                                         >  1:2327717/1‑149 (MQ=255)
                                                                                                  tCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATTCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATTCaaa                                                    >  1:2290569/1‑149 (MQ=255)
                                                                                                     gCAACCGCCTTTAATTATTTTTCAAAATCACTTTCCGGGCCTTGTTTGGTTTTTTTTTTCTTAATAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGt                                                 <  1:397593/149‑1 (MQ=255)
                                                                                                      cAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTa                                                <  2:706008/149‑1 (MQ=255)
                                                                                                      cAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCAGGTGTGGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTa                                                <  2:1711184/149‑1 (MQ=255)
                                                                                                        aCCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTAtt                                              <  2:3454971/149‑1 (MQ=255)
                                                                                                        aCCGCCGTTAATTCTTGTTCACCATCACTATCCCGCCCCTGTGTCGGTATTTTTTTCTTAATAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTAtt                                              <  2:3196274/149‑1 (MQ=255)
                                                                                                             ctttAATTCTTTTTCACAATCACTATCCCGGCCATGTGTCGGTATTTTTTTCTTAATAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTa                                         <  2:1360487/147‑1 (MQ=255)
                                                                                                             ctttAATTATTTTTCACCATCACTATCCCGGCCATGTGTCGGGTTTTTTTTCTTACTAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTa                                         <  1:2866331/147‑1 (MQ=255)
                                                                                                              gTTATTTCTTTTTCACCATCACTCTCCCGGCCTTGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTAt                                        <  1:3160858/149‑1 (MQ=255)
                                                                                                               ttAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATg                                       >  1:1967201/1‑149 (MQ=255)
                                                                                                               ttAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATg                                       >  1:3051499/1‑149 (MQ=255)
                                                                                                                      tttttCACCATCACTATCCCGGCCATGTATCGGGTTTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGtt                                <  1:181129/149‑1 (MQ=255)
                                                                                                                       ttttCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGttt                               <  1:4583072/149‑1 (MQ=255)
                                                                                                                         ttCACCATCACTATCCCGGCCTTGTGTCGTTTTTTTTTTCTAACAAAACACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTa                             <  1:2739748/149‑1 (MQ=255)
                                                                                                                           cACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGGGGTTTTaaa                           >  1:1211096/1‑149 (MQ=255)
                                                                                                                               aTCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGGTTTAAATTTa                       >  2:3377925/1‑149 (MQ=255)
                                                                                                                                tCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTAt                      >  2:4048097/1‑149 (MQ=255)
                                                                                                                                 caatATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTAtt                     <  1:4053653/146‑1 (MQ=255)
                                                                                                                                 cACTATCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTAtt                     <  2:861728/149‑1 (MQ=255)
                                                                                                                                 cACTATCCCGGCCATGTGTCGGTATTTTTTTCTTAATAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTAtt                     <  1:2149821/149‑1 (MQ=255)
                                                                                                                                 cACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTAtt                     <  1:1411207/149‑1 (MQ=255)
                                                                                                                                  aCTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTg                    >  2:3782479/1‑149 (MQ=255)
                                                                                                                                   cTATCCCGGCCCTGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGa                   <  2:4082040/149‑1 (MQ=255)
                                                                                                                                     aTCCCGGCCATGTTTGGTTATTTTTTTCTTAATAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATg                 <  1:2880298/149‑1 (MQ=255)
                                                                                                                                     aTCCCGGCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATg                 <  1:539797/149‑1 (MQ=255)
                                                                                                                                      tCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATgg                <  2:515342/149‑1 (MQ=255)
                                                                                                                                        ccGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTg              <  1:1940397/149‑1 (MQ=255)
                                                                                                                                         cGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTCTGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGa             >  2:2771987/1‑149 (MQ=255)
                                                                                                                                          ggCCATGTGTCGGTATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaa            <  1:3344374/149‑1 (MQ=255)
                                                                                                                                               tgtgTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatatta       >  2:2365887/1‑149 (MQ=255)
                                                                                                                                               tgtgTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatatta       >  1:1255379/1‑149 (MQ=255)
                                                                                                                                                gtgtCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatattaa      >  2:101787/1‑149 (MQ=255)
                                                                                                                                                   tCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGaatattaatat   >  2:3111298/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGGCGCGTTAACCTTGTATCTGGACTTCATCAACCTGTTCCTGATGTTGTTGCGGATCTTCGGCAACCGCCGTTAATTCTTTTTCACCATCACTATCCCGGCCATGTGTCGGGATTTTTTTCTTACTAAAAACGACACTTAACAAGTACCAGGTATAAAATATGCCTTATGGACGAGCGGAAGCGTTTTCTGTATCCATAAATGCAAAAGTATTTTGTATGCGTGGTTTTAAATTTATTGATGGTGAATATTAATATT  >  CP009273/815930‑816226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑