Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 971,532 A→T 11.4% Q152L (CAG→CTG)  mukE → chromosome condensin MukBEF, MukE localization factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273971,5320AT11.4% 989.4 / 93.8 297Q152L (CAG→CTG) mukEchromosome condensin MukBEF, MukE localization factor
Reads supporting (aligned to +/- strand):  ref base A (133/130);  new base T (18/16);  total (151/146)
Fisher's exact test for biased strand distribution p-value = 8.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

TCTCAGCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAG  >  CP009273/971386‑971680
                                                                                                                                                  |                                                                                                                                                    
  tcAGCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGgag                                                                                                                                                  >  1:3162284/1‑149 (MQ=255)
    aGCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGaa                                                                                                                                                <  1:3935481/149‑1 (MQ=255)
       ccGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGt                                                                                                                                             <  2:49781/149‑1 (MQ=255)
         ggAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTAc                                                                                                                                           >  1:4583901/1‑149 (MQ=255)
         ggAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTAc                                                                                                                                           <  1:1789180/149‑1 (MQ=255)
           aaCGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGt                                                                                                                                         >  2:4120905/1‑149 (MQ=255)
            aCGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGtt                                                                                                                                        >  2:1505886/1‑149 (MQ=255)
             cGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGttc                                                                                                                                       <  2:127123/149‑1 (MQ=255)
              ggCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGttct                                                                                                                                      >  1:4675700/1‑149 (MQ=255)
               gCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGttctt                                                                                                                                     <  1:160040/149‑1 (MQ=255)
               gCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGttctt                                                                                                                                     <  1:2473962/149‑1 (MQ=255)
                  ggCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGc                                                                                                                                  <  2:2314608/149‑1 (MQ=255)
                    cGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTc                                                                                                                                <  1:4340954/149‑1 (MQ=255)
                    cGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTc                                                                                                                                <  2:2993239/149‑1 (MQ=255)
                      aaTGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCaa                                                                                                                              >  2:2804010/1‑149 (MQ=255)
                       aTGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAAc                                                                                                                             <  1:3851848/149‑1 (MQ=255)
                       aTGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAAc                                                                                                                             >  2:2857162/1‑149 (MQ=255)
                        tGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAAcc                                                                                                                            >  1:2204790/1‑149 (MQ=255)
                         gAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCTACCg                                                                                                                           <  2:2676231/149‑1 (MQ=255)
                           ggggATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTc                                                                                                                         <  2:911315/149‑1 (MQ=255)
                           ggggATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTc                                                                                                                         >  2:4088358/1‑149 (MQ=255)
                            gggATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCt                                                                                                                        >  2:2507823/1‑149 (MQ=255)
                             ggATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTg                                                                                                                       <  1:3005673/149‑1 (MQ=255)
                                ttttCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGcgt                                                                                                                    >  1:1469429/1‑149 (MQ=255)
                                   tCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAACAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGcgtcgt                                                                                                                 <  2:2450321/149‑1 (MQ=255)
                                    cACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGtt                                                                                                                <  2:4279274/149‑1 (MQ=255)
                                    cACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGtt                                                                                                                >  1:1491654/1‑149 (MQ=255)
                                      cccAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTa                                                                                                              >  1:2160839/1‑149 (MQ=255)
                                       ccAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAg                                                                                                             <  1:1066177/149‑1 (MQ=255)
                                          gcagGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCa                                                                                                          <  2:1438903/149‑1 (MQ=255)
                                          gcagGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCa                                                                                                          <  2:611824/149‑1 (MQ=255)
                                            agGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATg                                                                                                        >  2:1960503/1‑149 (MQ=255)
                                            agGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATg                                                                                                        <  1:4210237/149‑1 (MQ=255)
                                             ggAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATgg                                                                                                       >  2:382179/1‑149 (MQ=255)
                                             ggAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATgg                                                                                                       <  1:2520917/149‑1 (MQ=255)
                                                aCTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGgtgt                                                                                                    >  2:3466785/1‑149 (MQ=255)
                                                aCTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTGGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGgtgt                                                                                                    <  2:3378001/149‑1 (MQ=255)
                                                 cTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGtgtg                                                                                                   >  2:1053244/1‑149 (MQ=255)
                                                 cTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGtgtg                                                                                                   >  2:3588914/1‑149 (MQ=255)
                                                     acgacgAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGttt                                                                                               >  1:1417917/1‑149 (MQ=255)
                                                      cgacgaACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTa                                                                                              <  1:3713825/149‑1 (MQ=255)
                                                      cgacgaACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTa                                                                                              <  2:2329755/149‑1 (MQ=255)
                                                       gacgaACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTAt                                                                                             >  2:1730672/1‑149 (MQ=255)
                                                       gacgaACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGAGTCGTTTAGGCATGGTGTGGTTTAt                                                                                             >  1:620100/1‑149 (MQ=255)
                                                        acgaACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATg                                                                                            >  2:2537164/1‑149 (MQ=255)
                                                        acgaACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATg                                                                                            <  2:3982549/149‑1 (MQ=255)
                                                          gaACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATggg                                                                                          <  2:1991007/149‑1 (MQ=255)
                                                          gaACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATggg                                                                                          <  1:1754258/149‑1 (MQ=255)
                                                            aCTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGcc                                                                                        <  1:3639055/149‑1 (MQ=255)
                                                             cTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCa                                                                                       >  2:372929/1‑149 (MQ=255)
                                                                cTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGa                                                                                    >  1:3342694/1‑149 (MQ=255)
                                                                 tCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGAc                                                                                   <  1:950289/149‑1 (MQ=255)
                                                                 tCACCCTGGCCGATGAAGCAAAACTGCCGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGAc                                                                                   <  1:1281447/149‑1 (MQ=255)
                                                                  cACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGAca                                                                                  <  1:2865433/149‑1 (MQ=255)
                                                                       tGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACagcagc                                                                             >  1:3490812/1‑149 (MQ=255)
                                                                        ggCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAgcagca                                                                            >  1:716669/1‑149 (MQ=255)
                                                                        ggCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAgcagca                                                                            <  2:2204790/149‑1 (MQ=255)
                                                                           cGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGt                                                                         >  1:2457140/1‑149 (MQ=255)
                                                                            gATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGtt                                                                        >  1:3311496/1‑149 (MQ=255)
                                                                             aTGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGttt                                                                       <  1:3763847/149‑1 (MQ=255)
                                                                              tGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTc                                                                      <  2:3238492/149‑1 (MQ=255)
                                                                              tGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTc                                                                      >  1:2341816/1‑149 (MQ=255)
                                                                              tGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTc                                                                      <  1:2818688/149‑1 (MQ=255)
                                                                                aaGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGc                                                                    >  2:1475006/1‑149 (MQ=255)
                                                                                 aGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCa                                                                   >  2:3641034/1‑149 (MQ=255)
                                                                                 aGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCa                                                                   >  2:313585/1‑149 (MQ=255)
                                                                                  gCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCAt                                                                  >  2:3493758/1‑149 (MQ=255)
                                                                                  gCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCAt                                                                  <  1:2493075/149‑1 (MQ=255)
                                                                                        ctgctgAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGa                                                            >  2:2824370/1‑149 (MQ=255)
                                                                                          gctgAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAAt                                                          <  2:3335511/149‑1 (MQ=255)
                                                                                          gctgAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAAt                                                          >  2:4578637/1‑149 (MQ=255)
                                                                                           ctgAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATc                                                         <  1:3065925/149‑1 (MQ=255)
                                                                                               aaCTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGgtg                                                     >  1:2046019/1‑149 (MQ=255)
                                                                                                  tGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTc                                                  <  2:3970792/149‑1 (MQ=255)
                                                                                                  tGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTc                                                  >  2:4243790/1‑149 (MQ=255)
                                                                                                     tGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGc                                               <  1:1829938/149‑1 (MQ=255)
                                                                                                     tGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGc                                               >  1:3522865/1‑149 (MQ=255)
                                                                                                      gAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCt                                              >  1:428867/1‑149 (MQ=255)
                                                                                                        acaacCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTc                                            >  1:741278/1‑149 (MQ=255)
                                                                                                         caacCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGGTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCg                                           >  2:3709716/1‑149 (MQ=255)
                                                                                                          aacCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCgg                                          >  2:2128621/1‑149 (MQ=255)
                                                                                                             cGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGc                                       <  1:1530313/149‑1 (MQ=255)
                                                                                                              gTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGcc                                      >  1:3251048/1‑149 (MQ=255)
                                                                                                               ttCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCg                                     >  2:1350704/1‑149 (MQ=255)
                                                                                                                 cAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAt                                   >  2:453894/1‑149 (MQ=255)
                                                                                                                  aaCCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtg                                  >  2:2091371/1‑149 (MQ=255)
                                                                                                                   aCCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt                                 <  1:2203154/149‑1 (MQ=255)
                                                                                                                   aCCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt                                 >  1:3124912/1‑149 (MQ=255)
                                                                                                                   aCCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgt                                 >  2:1568719/1‑149 (MQ=255)
                                                                                                                    ccGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGAtgtg                                <  2:3051173/149‑1 (MQ=255)
                                                                                                                          cAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCt                          <  2:573159/149‑1 (MQ=255)
                                                                                                                            gACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTgg                        >  1:2025114/1‑149 (MQ=255)
                                                                                                                                  gACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGcgacga                  >  1:1239210/1‑149 (MQ=255)
                                                                                                                                    ccGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATc                >  1:3816827/1‑149 (MQ=255)
                                                                                                                                     cGTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATcc               <  2:1417917/149‑1 (MQ=255)
                                                                                                                                      gTCAGAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATccc              >  2:442530/1‑149 (MQ=255)
                                                                                                                                        cAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCg            >  1:2708443/1‑149 (MQ=255)
                                                                                                                                          gAAGTTGCTGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTg          <  1:3466737/149‑1 (MQ=255)
                                                                                                                                          gAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTg          >  1:1107742/1‑149 (MQ=255)
                                                                                                                                               tGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGca     <  1:4578637/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCTCAGCCCGGAACGGCTGGCGAATGAGGGGATTTTCACCCAGCAGGAACTGTACGACGAACTGCTCACCCTGGCCGATGAAGCAAAACTGCTGAAACTGGTGAACAACCGTTCAACCGGTTCAGACGTTGACCGTCAGAAGTTGCAGGAGAAAGTACGTTCTTCGCTCAACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAG  >  CP009273/971386‑971680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑