Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 979,650 T→A 8.4% L182Q (CTG→CAG)  ycbL → putative metal‑binding enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273979,6500TA 8.4% 1029.2 / 67.1 296L182Q (CTG→CAG) ycbLputative metal‑binding enzyme
Reads supporting (aligned to +/- strand):  ref base T (131/140);  new base A (10/15);  total (141/155)
Fisher's exact test for biased strand distribution p-value = 5.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 297 total aligned reads displayed.

ACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGCTGGCCTGGATTTCTG  >  CP009273/979502‑979796
                                                                                                                                                    |                                                                                                                                                  
aCGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACt                                                                                                                                                    <  2:2463635/149‑1 (MQ=255)
  gCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTgg                                                                                                                                                  >  2:2344874/1‑149 (MQ=255)
    cGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACAgggg                                                                                                                                                >  2:3406981/1‑149 (MQ=255)
      ggTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGa                                                                                                                                              >  2:3625709/1‑149 (MQ=255)
          aTGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGAc                                                                                                                                          <  1:2347284/149‑1 (MQ=255)
          aTGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGAc                                                                                                                                          <  1:2321587/149‑1 (MQ=255)
          aTGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGAc                                                                                                                                          <  2:641589/149‑1 (MQ=255)
           tGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACg                                                                                                                                         >  1:1718461/1‑149 (MQ=255)
            gTCGTGTTTTTTGATGAGCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGt                                                                                                                                        <  2:3547700/149‑1 (MQ=255)
             tCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTg                                                                                                                                       >  1:4060295/1‑149 (MQ=255)
             tCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACAGGGGGATGACGTg                                                                                                                                       <  1:4295609/149‑1 (MQ=255)
               gtgtTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGat                                                                                                                                     <  1:1424318/149‑1 (MQ=255)
                   tttttGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGAtattt                                                                                                                                 <  2:3242823/149‑1 (MQ=255)
                      ttGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGAtatttatt                                                                                                                              <  2:1349626/149‑1 (MQ=255)
                       tgatgaTCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTc                                                                                                                             <  1:4380665/149‑1 (MQ=255)
                        gatgatCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTcc                                                                                                                            <  2:4509850/149‑1 (MQ=255)
                         atgatCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTACg                                                                                                                           >  2:3294988/1‑149 (MQ=255)
                          tgatCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCgg                                                                                                                          >  1:4268943/1‑149 (MQ=255)
                          tgatCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCgg                                                                                                                          <  1:716549/149‑1 (MQ=255)
                           gatCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCggg                                                                                                                         >  1:2224790/1‑149 (MQ=255)
                            atCGGGCTAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGt                                                                                                                        <  2:2970928/149‑1 (MQ=255)
                               gggCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCAc                                                                                                                     <  1:151076/149‑1 (MQ=255)
                               gggCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCAc                                                                                                                     >  2:3714347/1‑149 (MQ=255)
                                ggCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACg                                                                                                                    >  2:2624365/1‑149 (MQ=255)
                                  cAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGa                                                                                                                  >  2:2774986/1‑149 (MQ=255)
                                      gctgctGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAATATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCAt                                                                                                              >  1:500015/1‑149 (MQ=255)
                                        tgctgATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTa                                                                                                            <  1:1664004/149‑1 (MQ=255)
                                        tgctgATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTa                                                                                                            <  1:1761219/149‑1 (MQ=255)
                                          ctgATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATc                                                                                                          <  1:1933289/149‑1 (MQ=255)
                                          ctgATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACAGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATc                                                                                                          >  2:4475494/1‑149 (MQ=255)
                                                 cTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCATTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACtt                                                                                                   <  1:2019452/149‑1 (MQ=255)
                                                 cTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACtt                                                                                                   <  1:2943891/149‑1 (MQ=255)
                                                  tGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTg                                                                                                  >  1:3125178/1‑149 (MQ=255)
                                                   ggCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTgg                                                                                                 <  1:375791/149‑1 (MQ=255)
                                                   ggCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACAGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTgg                                                                                                 <  1:3674561/149‑1 (MQ=255)
                                                   ggCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACAGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTgg                                                                                                 <  1:2585063/149‑1 (MQ=255)
                                                      gATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTa                                                                                              >  2:2874476/1‑149 (MQ=255)
                                                        tGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATg                                                                                            <  2:1155582/149‑1 (MQ=255)
                                                        tGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATg                                                                                            >  1:208602/1‑149 (MQ=255)
                                                         gTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGa                                                                                           >  1:892066/1‑149 (MQ=255)
                                                         gTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGa                                                                                           >  2:3207503/1‑149 (MQ=255)
                                                           tATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAAc                                                                                         <  1:3294989/149‑1 (MQ=255)
                                                             ttttCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGc                                                                                       <  2:438286/149‑1 (MQ=255)
                                                               ttCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCt                                                                                     <  1:2632750/149‑1 (MQ=255)
                                                                 cAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGc                                                                                   >  2:1149319/1‑149 (MQ=255)
                                                                   aaGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCAt                                                                                 >  1:3852292/1‑149 (MQ=255)
                                                                     ggCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATaa                                                                               >  1:2039562/1‑149 (MQ=255)
                                                                     ggCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATaa                                                                               >  1:4568138/1‑149 (MQ=255)
                                                                          aGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCt                                                                          <  1:2573946/149‑1 (MQ=255)
                                                                           gTAGGGCGCAGTGACTTCCCGCGTGGCTATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCTTTATCCACACTTGGTTATGAACGCCTGCATAATCCCtt                                                                         <  1:2124250/149‑1 (MQ=255)
                                                                            tAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTc                                                                        >  2:2184565/1‑149 (MQ=255)
                                                                             aGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTcc                                                                       <  1:3249297/149‑1 (MQ=255)
                                                                                  gcAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCaa                                                                  <  1:510438/149‑1 (MQ=255)
                                                                                     gTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGAc                                                               <  2:1325050/149‑1 (MQ=255)
                                                                                      tGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACg                                                              <  2:1882704/149‑1 (MQ=255)
                                                                                        aCTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGaa                                                            <  1:3954041/149‑1 (MQ=255)
                                                                                          ttCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAAt                                                          <  2:69951/149‑1 (MQ=255)
                                                                                            cccGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGc                                                        >  2:900169/1‑149 (MQ=255)
                                                                                              cgcgTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGccc                                                      <  1:1153410/149‑1 (MQ=255)
                                                                                                 gTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACAGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTc                                                   <  1:1116934/149‑1 (MQ=255)
                                                                                                       aTCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCAACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTaa                                             >  1:1466208/1‑149 (MQ=255)
                                                                                                         cATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAgg                                           <  2:4154252/149‑1 (MQ=255)
                                                                                                            aaTCAACTGATTTCTAAAATCAAAGATAATTTGCTGCCAATGGTGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATTAACTCCTGCATAATCCCTTCCAGCAAGACGAAATGCCCGTCAGGAAAGGcac                                        >  2:3285144/1‑149 (MQ=255)
                                                                                                             aTCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGcaca                                       >  1:4497069/1‑149 (MQ=255)
                                                                                                               cAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATa                                     >  2:3801469/1‑149 (MQ=255)
                                                                                                                aaCTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATaa                                    >  2:2257822/1‑149 (MQ=255)
                                                                                                                 aCTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATaaa                                   <  1:1045179/149‑1 (MQ=255)
                                                                                                                 aCTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATaaa                                   >  1:4651297/1‑149 (MQ=255)
                                                                                                                 aCTGATTTCTTCAATCAAAGATAAATTGCTGCCACAGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATaaa                                   >  2:1743696/1‑149 (MQ=255)
                                                                                                                  cTGATTTCTTCAATCAAAGATAAATTGCTGCCACAGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATaaaa                                  <  1:3406981/149‑1 (MQ=255)
                                                                                                                   tGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATaaaaa                                 >  1:3864455/1‑149 (MQ=255)
                                                                                                                     aTTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAg                               <  2:1255018/149‑1 (MQ=255)
                                                                                                                      tttCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGc                              >  1:3181460/1‑149 (MQ=255)
                                                                                                                       ttcttcAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGcc                             >  2:4455395/1‑149 (MQ=255)
                                                                                                                       ttcttcAATCAAAGATAAATTGCTGCCACAGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGcc                             >  2:3382655/1‑149 (MQ=255)
                                                                                                                        tcttcAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGccc                            >  2:508699/1‑149 (MQ=255)
                                                                                                                        tcttcAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGccc                            >  2:2128504/1‑149 (MQ=255)
                                                                                                                        tcttcAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGccc                            >  2:4170130/1‑149 (MQ=255)
                                                                                                                            caatcaaAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCtt                        <  1:2227325/149‑1 (MQ=255)
                                                                                                                             aatcaaAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCttt                       >  2:1066735/1‑149 (MQ=255)
                                                                                                                             aatcaaAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCttt                       <  2:3522028/149‑1 (MQ=255)
                                                                                                                             aatcaaAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCttt                       <  1:4457586/149‑1 (MQ=255)
                                                                                                                              atcaaAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCtttt                      >  2:1562803/1‑149 (MQ=255)
                                                                                                                               tcaaAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTa                     <  2:1393286/149‑1 (MQ=255)
                                                                                                                                caaAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGATATGAACTCCAGCATAATCCCTTCCTGAAAGACTAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTATTaa                    >  2:607860/1‑149 (MQ=255)
                                                                                                                                caaAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTGGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTaa                    <  2:2671525/149‑1 (MQ=255)
                                                                                                                                 aaaGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAAt                   <  2:312609/149‑1 (MQ=255)
                                                                                                                                 aaaGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAAt                   >  1:4217908/1‑149 (MQ=255)
                                                                                                                                  aataTAAATTGCTGCCACAGGGTGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATg                  <  1:461008/146‑1 (MQ=255)
                                                                                                                                  aaGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATg                  >  1:903917/1‑149 (MQ=255)
                                                                                                                                  aaGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATg                  >  2:4357212/1‑149 (MQ=255)
                                                                                                                                   aGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGc                 >  1:3708449/1‑149 (MQ=255)
                                                                                                                                   aGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGc                 <  2:4610809/149‑1 (MQ=255)
                                                                                                                                    gATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGCt                >  2:3329930/1‑149 (MQ=255)
                                                                                                                                     aTAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGCTg               <  1:2359835/149‑1 (MQ=255)
                                                                                                                                      tAAATTGCTGCCACAGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGCTgg              <  2:2319115/149‑1 (MQ=255)
                                                                                                                                        aaTTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGCTGGcc            >  1:2448548/1‑149 (MQ=255)
                                                                                                                                        aaTTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGCTGGcc            >  1:2974192/1‑149 (MQ=255)
                                                                                                                                         aTTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGCTGGCCt           >  2:1616665/1‑149 (MQ=255)
                                                                                                                                           tgctgcCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGCTGGCCTgg         <  2:3099575/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACGCCGGGTCATGTCGTGTTTTTTGATGATCGGGCAAAGCTGCTGATTTCTGGCGATGTTATTTTCAAAGGCGGAGTAGGGCGCAGTGACTTCCCGCGTGGCGATCATAATCAACTGATTTCTTCAATCAAAGATAAATTGCTGCCACTGGGGGATGACGTGATATTTATTCCGGGTCACGGACCATTATCCACACTTGGTTATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGTCTGGTAAGGCACATAAAAAAGCCCGCTTTTAATGCTGGCCTGGATTTCTG  >  CP009273/979502‑979796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑