BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,222,207	C→T	8.3%	S51F (TCC→TTC)	ycgJ →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,222,207	0	C	T	8.3%	774.9 / 41.8	229	S51F (TCC→TTC)	ycgJ	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (99/111); new base T (5/14); total (104/125)
Fisher's exact test for biased strand distribution p-value = 9.53e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

Only 100 of 246 total aligned reads displayed.

AATATGATGCGTATTTTTTATATCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTT  >  CP009273/1222059‑1222352

aaTATGATGCGTATTTTTTATATCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTc                                                                                                                                                   <  2:4502504/149‑1 (MQ=255)

aaTATGATGCGTATTTTTTATATCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTc                                                                                                                                                   >  2:4454293/1‑149 (MQ=255)

aaTATGATGCGTATTTTTTATATCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTc                                                                                                                                                   >  2:3644706/1‑149 (MQ=255)

  tatGATGCGTATTTTTTATATCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTccc                                                                                                                                                 <  2:1357217/149‑1 (MQ=255)

  tatGATGCGTATTTTTTATATCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTccc                                                                                                                                                 <  2:2076333/149‑1 (MQ=255)

  tatGATGCGTATTTTTTATATCGGATTGTCAGGTGTGGGGATGATGTTCCCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCAATTccc                                                                                                                                                 >  2:4529065/1‑149 (MQ=255)

   atgatgCGTATTTTTTATATCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTcccc                                                                                                                                                >  2:2199110/1‑149 (MQ=255)

     gatgCGTATTTTTTATATCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCag                                                                                                                                              >  2:1002127/1‑149 (MQ=255)

       tgCGTATTTTTTATATCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCagag                                                                                                                                            <  1:1642330/149‑1 (MQ=255)

        gCGTATTTTTTATATCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGt                                                                                                                                           <  1:2670570/149‑1 (MQ=255)

          gTATTTTTTATATCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTa                                                                                                                                         >  2:2247181/1‑149 (MQ=255)

           tATTTTTTATATCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTaa                                                                                                                                        <  2:3528070/149‑1 (MQ=255)

                    tatCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTTCCCAGAGTTAACAAGGAAat                                                                                                                               >  2:1465846/1‑149 (MQ=255)

                    tatCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTTCCCAGAGTTAACAAGGAAat                                                                                                                               >  2:82460/1‑149 (MQ=255)

                    tatCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAat                                                                                                                               <  2:1068914/149‑1 (MQ=255)

                    tatCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGAATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGACat                                                                                                                               >  2:657187/1‑149 (MQ=255)

                     atCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAata                                                                                                                              >  1:4079054/1‑149 (MQ=255)

                      tCGGATTGTCAGGTGTGTGGATGATGATCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATATCCCCAGAGTTAACAAGGAAatat                                                                                                                             >  2:3354476/1‑149 (MQ=255)

                      tCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAatat                                                                                                                             >  1:1132241/1‑149 (MQ=255)

                      tCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAatat                                                                                                                             <  1:1080770/149‑1 (MQ=255)

                        ggATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATAtct                                                                                                                           <  2:3278502/149‑1 (MQ=255)

                         gATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGACATAtctc                                                                                                                          >  2:813268/1‑149 (MQ=255)

                         gATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATAtctc                                                                                                                          <  1:3104300/149‑1 (MQ=255)

                           ttGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCgg                                                                                                                        >  2:313375/1‑149 (MQ=255)

                             gTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCg                                                                                                                      >  2:2659990/1‑149 (MQ=255)

                              tCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGATATATCTCGGCGa                                                                                                                     <  2:3050197/149‑1 (MQ=255)

                              tCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGa                                                                                                                     <  1:1980140/149‑1 (MQ=255)

                               cAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGaa                                                                                                                    >  1:479937/1‑149 (MQ=255)

                                aGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGaaa                                                                                                                   <  1:3064289/149‑1 (MQ=255)

                                aGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGaaa                                                                                                                   >  2:26411/1‑149 (MQ=255)

                                  gtgtGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGaaaaa                                                                                                                 >  2:3205481/1‑149 (MQ=255)

                                           tgatgTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAACAAGCCGCTGaa                                                                                                        >  2:3727346/1‑149 (MQ=255)

                                            gatgTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGaaa                                                                                                       <  2:4008126/149‑1 (MQ=255)

                                              tgTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAAc                                                                                                     >  2:1632251/1‑149 (MQ=255)

                                              tgTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAAc                                                                                                     >  2:766907/1‑149 (MQ=255)

                                                  ctcATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTAc                                                                                                 >  1:1608216/1‑149 (MQ=255)

                                                   tcatcaATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTTCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACa                                                                                                >  1:1687605/1‑149 (MQ=255)

                                                     atcaATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAAt                                                                                              <  1:971101/149‑1 (MQ=255)

                                                     atcaATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAAt                                                                                              >  2:2397104/1‑149 (MQ=255)

                                                      tcaATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATc                                                                                             <  1:1942919/149‑1 (MQ=255)

                                                        aaTGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCATATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCAt                                                                                           <  1:138723/149‑1 (MQ=255)

                                                         aTGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGACATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCAtt                                                                                          >  2:669414/1‑149 (MQ=255)

                                                         aTGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCAtt                                                                                          >  2:1960353/1‑149 (MQ=255)

                                                         aTGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCAtt                                                                                          >  2:1236730/1‑149 (MQ=255)

                                                              gTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAg                                                                                     <  1:4249669/149‑1 (MQ=255)

                                                                cTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGc                                                                                   <  1:3758078/149‑1 (MQ=255)

                                                                cTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCATATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGc                                                                                   <  1:2119094/149‑1 (MQ=255)

                                                                  ggAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTa                                                                                 <  1:4566103/149‑1 (MQ=255)

                                                                   gAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTAc                                                                                <  2:3868499/149‑1 (MQ=255)

                                                                   gAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTAc                                                                                <  1:2778830/149‑1 (MQ=255)

                                                                    aaaTGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTTCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCAGAAAACTTACAATCATTACAAGGCTACg                                                                               >  1:2370547/1‑149 (MQ=255)

                                                                     aaTGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGa                                                                              >  2:3460428/1‑149 (MQ=255)

                                                                        gACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATcc                                                                           >  2:2847336/1‑149 (MQ=255)

                                                                           gCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAg                                                                        <  2:4566732/149‑1 (MQ=255)

                                                                                  gACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTTCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAAttt                                                                 <  1:668570/149‑1 (MQ=255)

                                                                                  gACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAAttt                                                                 <  1:485299/149‑1 (MQ=255)

                                                                                  gACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAAttt                                                                 >  2:489768/1‑149 (MQ=255)

                                                                                   aCTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTa                                                                >  2:1840529/1‑149 (MQ=255)

                                                                                    cTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAAttttc                                                               <  2:721481/149‑3 (MQ=255)

                                                                                      tCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACAt                                                             >  1:2402073/1‑149 (MQ=255)

                                                                                       cAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACAtt                                                            <  2:4299463/149‑1 (MQ=255)

                                                                                       cAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACAtt                                                            <  2:452178/149‑1 (MQ=255)

                                                                                        aaTCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTc                                                           <  1:1491331/149‑1 (MQ=255)

                                                                                         aTCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCg                                                          >  1:1821493/1‑149 (MQ=255)

                                                                                         aTCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCg                                                          >  1:3177020/1‑149 (MQ=255)

                                                                                          tctcCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGc                                                         <  2:2300892/149‑1 (MQ=255)

                                                                                                  gTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGgtg                                                 <  1:4338733/149‑1 (MQ=255)

                                                                                                   tGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACTAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGgtgt                                                <  1:4633506/149‑1 (MQ=255)

                                                                                                    gcggcgTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTTCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTa                                               <  1:3255391/149‑1 (MQ=255)

                                                                                                    gcggcgTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGATATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTa                                               <  1:4394475/149‑1 (MQ=255)

                                                                                                      ggcgTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTAtt                                             <  2:4484883/149‑1 (MQ=255)

                                                                                                      ggcgTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTAtt                                             >  2:447247/1‑149 (MQ=255)

                                                                                                       gcgTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTCACGGTGTAttt                                            >  2:378921/1‑149 (MQ=255)

                                                                                                          ttgttTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTTCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGc                                         >  2:3011410/1‑149 (MQ=255)

                                                                                                            gttTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGa                                       <  1:3181716/149‑1 (MQ=255)

                                                                                                             tttGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGAt                                      <  1:4153960/149‑1 (MQ=255)

                                                                                                              ttGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATg                                     >  1:1331140/1‑149 (MQ=255)

                                                                                                               tGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGt                                    <  2:413349/149‑1 (MQ=255)

                                                                                                                gCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGtt                                   <  1:1233954/149‑1 (MQ=255)

                                                                                                                gCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGtt                                   <  1:3963416/149‑1 (MQ=255)

                                                                                                                gCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGtt                                   >  2:4131899/1‑149 (MQ=255)

                                                                                                                gCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGtt                                   <  1:1149371/149‑1 (MQ=255)

                                                                                                                  gACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTaa                                 >  2:1749691/1‑149 (MQ=255)

                                                                                                                    cccGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAg                               >  1:587504/1‑149 (MQ=255)

                                                                                                                    cccGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAg                               >  1:2927143/1‑149 (MQ=255)

                                                                                                                     ccGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGa                              <  1:3472984/149‑1 (MQ=255)

                                                                                                                      cGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGaa                             >  1:3642407/1‑149 (MQ=255)

                                                                                                                      cGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGaa                             <  2:2823866/149‑1 (MQ=255)

                                                                                                                          tatTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAAt                         <  1:879074/149‑1 (MQ=255)

                                                                                                                            tttGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTa                       >  2:4235450/1‑149 (MQ=255)

                                                                                                                            tttGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTa                       >  1:2565877/1‑149 (MQ=255)

                                                                                                                              tgtgtgAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATg                     <  2:900989/149‑1 (MQ=255)

                                                                                                                               gtgtgAACTCAGATGGCATTTTCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGt                    <  2:500292/149‑1 (MQ=255)

                                                                                                                               gtgtgAACTCAGATGGCATTTTCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGt                    <  2:864243/149‑1 (MQ=255)

                                                                                                                               gtgtgAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGt                    >  1:4020331/1‑149 (MQ=255)

                                                                                                                               gtgtgAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGt                    >  1:3895247/1‑149 (MQ=255)

                                                                                                                                tgtgAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTc                   >  1:360842/1‑149 (MQ=255)

                                                                                                                                  tgAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGt                 >  2:1212320/1‑149 (MQ=255)

                                                                                                                                     aCTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGtgat              >  2:2468300/1‑149 (MQ=255)

                                                                                                                                              gCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTAt     <  1:4454293/149‑1 (MQ=255)

AATATGATGCGTATTTTTTATATCGGATTGTCAGGTGTGGGGATGATGTTCTCATCAATGGCGTCTGGAAATGACGCTGGCGGACTTCAATCTCCGGCGTGCGGCGTTGTTTGCGACCCGTATATTTGTGTGAACTCAGATGGCATTTCCCCAGAGTTAACAAGGAAATATCTCGGCGAAAAAGCCGCTGAAAACTTACAATCATTACAAGGCTACGATCCCAGCGAATTTACATTCGCTAACGGTGTATTTTGCGATGTTAAAGAAAAATTATGTCGTGATGATCGTTATTTT  >  CP009273/1222059‑1222352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`