Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,228,879 A→C 10.3% L432R (CTG→CGG)  nhaB ← sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,228,8790AC10.3% 797.2 / 78.6 261L432R (CTG→CGG) nhaBsodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (114/120);  new base C (12/15);  total (126/135)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

GTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCGATGC  >  CP009273/1228731‑1229027
                                                                                                                                                    |                                                                                                                                                    
gtAAGGCAGGGCCATCCACACCATGCGGCCATTAGAGAGGCGAATCAATGGCGCGAGTGCAGATGTCAGCAGGAACAGGAACGCATCCTGACCGTTCGGCGTAGCGACGGAGGTCAGATTGGTACCGGTATTAATGGCGACCGCcagca                                                                                                                                                      <  1:47189/149‑1 (MQ=255)
   aGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCt                                                                                                                                                   <  2:3281594/149‑1 (MQ=255)
    ggCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTc                                                                                                                                                  <  1:213916/149‑1 (MQ=255)
    ggCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTc                                                                                                                                                  <  1:2297593/149‑1 (MQ=255)
       aGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTa                                                                                                                                               >  1:2248594/1‑149 (MQ=255)
            cATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCCGCTCGTATTGCt                                                                                                                                          <  1:2794758/149‑1 (MQ=255)
            cATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCt                                                                                                                                          <  1:4336017/149‑1 (MQ=255)
            cATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCt                                                                                                                                          <  1:4197981/149‑1 (MQ=255)
             aTCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCtt                                                                                                                                         >  2:3168083/1‑149 (MQ=255)
              tCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTc                                                                                                                                        <  2:2287201/149‑1 (MQ=255)
                cacaCCATGCGGCCATCAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCaa                                                                                                                                      <  1:2074637/149‑1 (MQ=255)
                cacaCCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCCGCTCGTATTGCTTCaa                                                                                                                                      <  1:983769/149‑1 (MQ=255)
                cacaCCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCaa                                                                                                                                      <  1:2519979/149‑1 (MQ=255)
                  cacCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCCGCTCGTATTGCTTCAACg                                                                                                                                    >  1:1155362/1‑149 (MQ=255)
                   acCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCCGCTCGTATTGCTTCAACGt                                                                                                                                   <  1:312208/149‑1 (MQ=255)
                   acCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGt                                                                                                                                   <  2:2940979/149‑1 (MQ=255)
                      aTGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCCGCTCGTATTGCTTCAACGTGAt                                                                                                                                >  2:521847/1‑149 (MQ=255)
                           gCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAgcgc                                                                                                                           <  2:1682895/149‑1 (MQ=255)
                              aTAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCCGCTCGTATTGCTTCAACGTGATAGCGCCAc                                                                                                                        >  1:1834178/1‑149 (MQ=255)
                              aTAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCAc                                                                                                                        >  2:4569478/1‑149 (MQ=255)
                                 agagagGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACttt                                                                                                                     >  2:315570/1‑149 (MQ=255)
                                  gagagGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTc                                                                                                                    <  1:4521171/149‑1 (MQ=255)
                                   agagGCGAATCACTGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTcc                                                                                                                   <  1:445547/149‑1 (MQ=255)
                                   agagGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTcc                                                                                                                   <  2:1351002/149‑1 (MQ=255)
                                     agGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCAt                                                                                                                 <  1:1960494/149‑1 (MQ=255)
                                     agGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCAt                                                                                                                 <  1:636831/149‑1 (MQ=255)
                                      ggCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCCGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCAtt                                                                                                                <  2:3522214/149‑1 (MQ=255)
                                      ggCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCAtt                                                                                                                >  1:1768945/1‑149 (MQ=255)
                                         gAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGcc                                                                                                             <  1:1987699/149‑1 (MQ=255)
                                          aaTCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCg                                                                                                            >  1:294200/1‑149 (MQ=255)
                                           aTCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGc                                                                                                           >  1:702444/1‑149 (MQ=255)
                                           aTCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGc                                                                                                           >  1:1645943/1‑149 (MQ=255)
                                              aaTGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCttt                                                                                                        >  1:4149619/1‑149 (MQ=255)
                                               aTGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTc                                                                                                       >  2:1215392/1‑149 (MQ=255)
                                                 ggCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGc                                                                                                     <  1:2173281/149‑1 (MQ=255)
                                                 ggCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGc                                                                                                     >  2:3353587/1‑149 (MQ=255)
                                                  gcgcGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCt                                                                                                    <  2:1091152/149‑1 (MQ=255)
                                                    gcgAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTc                                                                                                  >  1:3670434/1‑149 (MQ=255)
                                                    gcgAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTc                                                                                                  <  1:706694/149‑1 (MQ=255)
                                                      gAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGt                                                                                                >  2:879402/1‑149 (MQ=255)
                                                      gAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGt                                                                                                <  2:2012454/149‑1 (MQ=255)
                                                        gTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAAGGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTg                                                                                              <  2:3266706/149‑1 (MQ=255)
                                                          gCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGat                                                                                            >  1:3570866/1‑149 (MQ=255)
                                                           cAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGata                                                                                           >  2:2698940/1‑149 (MQ=255)
                                                            agagGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGatat                                                                                          <  2:2586024/149‑1 (MQ=255)
                                                             gagGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGAtata                                                                                         >  2:253982/1‑149 (MQ=255)
                                                               ggTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATCGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATaaa                                                                                       >  2:2091351/1‑149 (MQ=255)
                                                               ggTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATaaa                                                                                       >  2:2922374/1‑149 (MQ=255)
                                                                 tCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATc                                                                                     <  1:1497698/149‑1 (MQ=255)
                                                                  cagcagGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCg                                                                                    <  2:4199949/149‑1 (MQ=255)
                                                                   agcagGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGt                                                                                   >  2:2388756/1‑149 (MQ=255)
                                                                    gcagGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTc                                                                                  <  2:2541105/149‑1 (MQ=255)
                                                                      aggaacaggaacGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGGTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTccc                                                                                <  2:620012/149‑1 (MQ=255)
                                                                       ggaacaggaacGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTcccc                                                                               >  1:2054904/1‑149 (MQ=255)
                                                                        gaacaggaacGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCa                                                                              <  1:1943761/149‑1 (MQ=255)
                                                                              gaacGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGa                                                                        <  1:566533/149‑1 (MQ=255)
                                                                              gaacGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGa                                                                        >  1:3748972/1‑149 (MQ=255)
                                                                              gaacGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGa                                                                        <  2:1937639/149‑1 (MQ=255)
                                                                               aacGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGAc                                                                       <  2:4123438/149‑1 (MQ=255)
                                                                               aacGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGAGTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGAc                                                                       <  2:1632241/149‑1 (MQ=255)
                                                                                acGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACg                                                                      >  2:3314299/1‑149 (MQ=255)
                                                                                acGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACg                                                                      >  1:255525/1‑149 (MQ=255)
                                                                                 cGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCCGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGt                                                                     <  2:4444535/149‑1 (MQ=255)
                                                                                      ccTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATcc                                                                <  1:1014547/149‑1 (MQ=255)
                                                                                         gACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGaa                                                             >  2:2205845/1‑149 (MQ=255)
                                                                                         gACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGaa                                                             >  1:3395573/1‑149 (MQ=255)
                                                                                          aCCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCCGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATACGaaa                                                            >  2:537591/1‑149 (MQ=255)
                                                                                          aCCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGaaa                                                            <  1:1556460/149‑1 (MQ=255)
                                                                                           ccGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAAt                                                           >  1:3345754/1‑149 (MQ=255)
                                                                                           ccGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAAt                                                           <  1:1210085/149‑1 (MQ=255)
                                                                                            cGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCCGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATg                                                          <  1:1105713/149‑1 (MQ=255)
                                                                                                    gTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGAcag                                                  <  2:3437845/149‑1 (MQ=255)
                                                                                                    gTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGAcag                                                  >  2:1536853/1‑149 (MQ=255)
                                                                                                      aGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAAAGGATGAcagca                                                >  2:4273724/1‑149 (MQ=255)
                                                                                                       gCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGAcagcag                                               >  2:105986/1‑149 (MQ=255)
                                                                                                        cGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGa                                              <  1:2263272/149‑1 (MQ=255)
                                                                                                             gAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCCGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGtt                                         >  2:927363/1‑149 (MQ=255)
                                                                                                                    gATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAata                                  >  2:3972408/1‑149 (MQ=255)
                                                                                                                    gATTGGGACCGGTATTACTGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAata                                  <  2:4524928/149‑1 (MQ=255)
                                                                                                                         gTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGaa                             >  1:1445721/1‑149 (MQ=255)
                                                                                                                         gTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGaa                             >  1:3782561/1‑149 (MQ=255)
                                                                                                                          tACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAAc                            >  2:2992907/1‑149 (MQ=255)
                                                                                                                          tACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAAc                            <  2:762791/149‑1 (MQ=255)
                                                                                                                          tACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAAc                            <  1:3976836/149‑1 (MQ=255)
                                                                                                                            ccGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAg                          >  2:1191829/1‑149 (MQ=255)
                                                                                                                            ccGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGAAGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATATAACat                          >  2:450864/1‑148 (MQ=255)
                                                                                                                             cGGTATTAATGGCGACCGCCAGCCGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGc                         <  1:1901459/149‑1 (MQ=255)
                                                                                                                               gTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGa                       >  2:714935/1‑149 (MQ=255)
                                                                                                                               gTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGa                       >  2:3928167/1‑149 (MQ=255)
                                                                                                                               gTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGa                       >  1:2725786/1‑149 (MQ=255)
                                                                                                                                   tAATGGCGACCGCCAGCAGCTCGTTTTGCTTCAACTTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGTTGACAGCAGACCGTTGAAAATATAGAACAGCGACAGc                   <  1:3701600/149‑1 (MQ=255)
                                                                                                                                      tGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGCTGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGa                >  1:1717847/1‑149 (MQ=255)
                                                                                                                                      tGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGa                >  1:1026621/1‑149 (MQ=255)
                                                                                                                                      tGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGa                >  1:2566170/1‑149 (MQ=255)
                                                                                                                                       ggCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAg               >  2:244576/1‑149 (MQ=255)
                                                                                                                                            ccGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGt          <  1:3476836/149‑1 (MQ=255)
                                                                                                                                               ccAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTcc       >  1:874880/1‑149 (MQ=255)
                                                                                                                                               ccAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTcc       <  2:3035190/149‑1 (MQ=255)
                                                                                                                                                cagcagCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCg      <  2:3789092/149‑1 (MQ=255)
                                                                                                                                                    agcTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCGATGc  >  2:4609232/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTAAGGCAGGGCCATCCACACCATGCGGCCATAAGAGAGGCGAATCAATGGCGCGAGTGCAGAGGTCAGCAGGAACAGGAACGCAGCCTGACCGTTCGGCGTAGCGACGGAGGGCAGATTGGTACCGGTATTAATGGCGACCGCCAGCAGCTCGTATTGCTTCAACGTGATAGCGCCACTTTCCATTGCCGCTTTCGCTTCGTTGATATAAATCGTCCCCACGAAGACGTTATCCGAAATGGATGACAGCAGACCGTTGAAAATATAGAACAGCGACAGCTGAGCATGTTCCGATGC  >  CP009273/1228731‑1229027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑