Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,229,460 G→T 15.6% F238L (TTC→TTA)  nhaB ← sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,229,4600GT15.6% 1052.7 / 137.1 327F238L (TTC→TTA) nhaBsodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (151/125);  new base T (28/23);  total (179/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 346 total aligned reads displayed.

GGCTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTT  >  CP009273/1229312‑1229608
                                                                                                                                                    |                                                                                                                                                    
ggcTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGgaag                                                                                                                                                      >  2:3396255/1‑149 (MQ=255)
 gcTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGgaaga                                                                                                                                                     >  1:2600261/1‑149 (MQ=255)
   tttGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATaaa                                                                                                                                                   <  1:4179717/149‑1 (MQ=255)
    ttGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAAt                                                                                                                                                  <  2:1096902/149‑1 (MQ=255)
    ttGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAAt                                                                                                                                                  <  2:1188099/149‑1 (MQ=255)
             aaaCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAAt                                                                                                                                         >  2:1262837/1‑149 (MQ=255)
             aaaCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAAt                                                                                                                                         >  2:1692528/1‑149 (MQ=255)
              aaCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATg                                                                                                                                        >  2:1816973/1‑149 (MQ=255)
               aCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgc                                                                                                                                       >  2:3294866/1‑149 (MQ=255)
                cTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCGGGAAGAAATCGCCAAAATgcc                                                                                                                                      >  1:646500/1‑149 (MQ=255)
                    tGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgccagcc                                                                                                                                  <  1:2437828/149‑1 (MQ=255)
                     gCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgccagcca                                                                                                                                 <  1:722652/149‑1 (MQ=255)
                      cAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGccagccag                                                                                                                                <  2:3639599/149‑1 (MQ=255)
                        acacTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCagccagcc                                                                                                                              >  2:2798704/1‑149 (MQ=255)
                            ttCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCtt                                                                                                                          <  1:2171771/149‑1 (MQ=255)
                            ttCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCtt                                                                                                                          >  1:2262633/1‑149 (MQ=255)
                              cgcgGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTa                                                                                                                        <  1:1696832/149‑1 (MQ=255)
                                 ggACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCg                                                                                                                     >  2:1537060/1‑149 (MQ=255)
                                  gACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCga                                                                                                                    <  1:2562135/149‑1 (MQ=255)
                                   aCTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCgat                                                                                                                   >  2:2332780/1‑149 (MQ=255)
                                    cTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCgatg                                                                                                                  >  1:1758390/1‑149 (MQ=255)
                                    cTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCGACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCgatg                                                                                                                  <  1:3759236/149‑1 (MQ=255)
                                     tttCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCgatga                                                                                                                 <  2:542654/149‑1 (MQ=255)
                                           cGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGt                                                                                                           >  1:3427075/1‑149 (MQ=255)
                                            ggCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGtt                                                                                                          >  1:1451124/1‑149 (MQ=255)
                                            ggCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGtt                                                                                                          >  1:388741/1‑149 (MQ=255)
                                              cAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCt                                                                                                        <  1:503492/149‑1 (MQ=255)
                                                   tttCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGt                                                                                                   >  1:4424781/1‑149 (MQ=255)
                                                    ttCACCTTAGCCAAACCATCGCAGCTTCACTACCAGCAGGAAGGTTAACAGGCCACAATTCAGAACCGGATCGGTCACCGGCGACATGCGCATGAATAAATCGCCAAAATGACAGCCATCCGCTTTAGCGATGATCAGGTTCTGTGGtt                                                                                                  <  1:4643492/149‑1 (MQ=255)
                                                        ccGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGcc                                                                                              <  2:2910346/149‑1 (MQ=255)
                                                          gTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCcca                                                                                            >  2:3157945/1‑149 (MQ=255)
                                                              ccAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCAt                                                                                        >  2:384122/1‑149 (MQ=255)
                                                               cAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATg                                                                                       >  2:633635/1‑149 (MQ=255)
                                                               cAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATg                                                                                       <  1:1867845/149‑1 (MQ=255)
                                                                 aaCCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGt                                                                                     >  2:2701882/1‑149 (MQ=255)
                                                                 aaCCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGt                                                                                     <  1:4052103/149‑1 (MQ=255)
                                                                  aCCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTc                                                                                    >  1:102383/1‑149 (MQ=255)
                                                                   ccAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCa                                                                                   <  1:3186861/149‑1 (MQ=255)
                                                                   ccAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCa                                                                                   <  2:377932/149‑1 (MQ=255)
                                                                    cAACGCAGCTTCTCTCCCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCAt                                                                                  <  1:148537/149‑1 (MQ=255)
                                                                     aaCGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCAtt                                                                                 >  2:1963334/1‑149 (MQ=255)
                                                                     aaCGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCAtt                                                                                 <  1:3941577/149‑1 (MQ=255)
                                                                     aaCGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCAtt                                                                                 >  1:3589533/1‑149 (MQ=255)
                                                                        gCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTAcg                                                                              >  2:4677513/1‑149 (MQ=255)
                                                                            cTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTAcgccgc                                                                          <  1:813847/149‑1 (MQ=255)
                                                                             ttCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACgccgcc                                                                         <  2:665235/149‑1 (MQ=255)
                                                                                  tACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATg                                                                    <  1:2160453/149‑1 (MQ=255)
                                                                                  tACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATg                                                                    >  1:3149411/1‑149 (MQ=255)
                                                                                   aCCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGc                                                                   <  1:3184054/149‑1 (MQ=255)
                                                                                     cagcagGCAGGTTAACAGGCCACAAATCAGTACCGGAACGGTCAGCGGCGACATGCGCAGGATGATATCGCCAAAATGCCAGCCAGCCGCTTTAGCGTTGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCgg                                                                 <  2:418329/149‑1 (MQ=255)
                                                                                     cagcagGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCgg                                                                 <  2:4103449/149‑1 (MQ=255)
                                                                                     cagcagGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCgg                                                                 >  2:1392415/1‑149 (MQ=255)
                                                                                      agcagGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGt                                                                <  1:3395542/149‑1 (MQ=255)
                                                                                       gcaggcagGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTg                                                               >  2:3119455/1‑149 (MQ=255)
                                                                                       gcaggcagGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTg                                                               <  2:2568765/149‑1 (MQ=255)
                                                                                         aggcaggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGcc                                                             >  1:2827406/1‑149 (MQ=255)
                                                                                            caggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGac                                                          <  2:1431894/149‑1 (MQ=255)
                                                                                                   aCAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCGAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGgca                                                   >  1:3871640/1‑149 (MQ=255)
                                                                                                      ggCCACAAATCAGAACCGGAACGGTCACCGGCGACCTGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCAGTACGCCGCCTAATGCGGTGCCGACACCGgcatgc                                                >  2:4537573/1‑149 (MQ=255)
                                                                                                      ggCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGgcatgc                                                <  2:888671/149‑1 (MQ=255)
                                                                                                        ccACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGgcatgcat                                              >  1:1667157/1‑149 (MQ=255)
                                                                                                             aaTCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCAtcatca                                         >  2:2229406/1‑149 (MQ=255)
                                                                                                                cAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGc                                      <  2:799093/149‑1 (MQ=255)
                                                                                                                cAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGc                                      <  1:1351395/149‑1 (MQ=255)
                                                                                                                 aGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCt                                     >  2:1646176/1‑149 (MQ=255)
                                                                                                                  gAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTa                                    >  2:298830/1‑149 (MQ=255)
                                                                                                                  gAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTa                                    >  1:2229124/1‑149 (MQ=255)
                                                                                                                  gAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTa                                    <  1:1196213/149‑1 (MQ=255)
                                                                                                                  gAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTa                                    >  2:3127038/1‑149 (MQ=255)
                                                                                                                    aCCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACg                                  >  1:4257259/1‑149 (MQ=255)
                                                                                                                     ccGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGc                                 >  1:1096568/1‑149 (MQ=255)
                                                                                                                      cGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCa                                <  2:1786512/149‑1 (MQ=255)
                                                                                                                       ggAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAg                               >  1:2303332/1‑149 (MQ=255)
                                                                                                                        gAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGa                              >  1:200147/1‑149 (MQ=255)
                                                                                                                        gAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGa                              >  2:1443218/1‑149 (MQ=255)
                                                                                                                           cGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAg                           >  1:2079303/1‑149 (MQ=255)
                                                                                                                            ggTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGc                          <  1:4025199/149‑1 (MQ=255)
                                                                                                                               cACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCAc                       >  1:919660/1‑149 (MQ=255)
                                                                                                                               cACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCAc                       <  1:842079/149‑1 (MQ=255)
                                                                                                                               cACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCAc                       <  1:3550536/149‑1 (MQ=255)
                                                                                                                               cACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCAc                       >  2:3952081/1‑149 (MQ=255)
                                                                                                                               cACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCAc                       >  1:3502273/1‑149 (MQ=255)
                                                                                                                                aCCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACg                      >  1:4236604/1‑149 (MQ=255)
                                                                                                                                 ccGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCTGTGACGACACCGGCAAGCATCATCAGTCTACGCAGAAAGCCACgg                     >  1:1565303/1‑149 (MQ=255)
                                                                                                                                 ccGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACgg                     >  1:2707741/1‑149 (MQ=255)
                                                                                                                                 ccGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACgg                     >  1:4499415/1‑149 (MQ=255)
                                                                                                                                   ggCGACATGCGGAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGaa                   >  2:3371334/1‑149 (MQ=255)
                                                                                                                                   ggCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGaa                   <  2:104927/149‑1 (MQ=255)
                                                                                                                                    gCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGTGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAAc                  >  2:1583519/1‑149 (MQ=255)
                                                                                                                                      gACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTg                >  2:3753868/1‑149 (MQ=255)
                                                                                                                                      gACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTg                >  2:2817960/1‑149 (MQ=255)
                                                                                                                                      gACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCAGGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTg                <  2:4359449/149‑1 (MQ=255)
                                                                                                                                       aCATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGt               <  2:4527505/149‑1 (MQ=255)
                                                                                                                                       aCATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGt               <  2:3580237/149‑1 (MQ=255)
                                                                                                                                        cATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGtt              <  2:2600261/149‑1 (MQ=255)
                                                                                                                                         aTGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTc             >  1:3963302/1‑149 (MQ=255)
                                                                                                                                              cAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGaa        <  1:535279/149‑1 (MQ=255)
                                                                                                                                                ggAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAAcc      <  2:2132618/149‑1 (MQ=255)
                                                                                                                                                    gaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACtt  <  1:870806/149‑1 (MQ=255)
                                                                                                                                                    gaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACtt  <  1:1816551/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACTT  >  CP009273/1229312‑1229608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑