Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,679,204 T→A 7.7% I230N (ATC→AAC)  tus → inhibitor of replication at Ter, DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,679,2040TA 7.7% 624.1 / 2.5 204I230N (ATC→AAC) tusinhibitor of replication at Ter, DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (111/72);  new base A (2/13);  total (113/91)
Fisher's exact test for biased strand distribution p-value = 5.54e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

ACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAAT  >  CP009273/1679058‑1679352
                                                                                                                                                  |                                                                                                                                                    
aCATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCa                                                                                                                                                    >  2:2855039/1‑149 (MQ=255)
 cATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCaa                                                                                                                                                   >  1:3292085/1‑149 (MQ=255)
 cATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCaa                                                                                                                                                   >  1:2532737/1‑149 (MQ=255)
 cATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCaa                                                                                                                                                   >  1:955706/1‑149 (MQ=255)
  aTCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCaaa                                                                                                                                                  <  1:3801756/149‑1 (MQ=255)
   tCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAAc                                                                                                                                                 >  1:1055583/1‑149 (MQ=255)
     gTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGt                                                                                                                                               >  2:2078548/1‑149 (MQ=255)
          gaAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGt                                                                                                                                          >  2:1425867/1‑149 (MQ=255)
             gTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGaa                                                                                                                                       >  1:1091719/1‑149 (MQ=255)
                cTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGTGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGt                                                                                                                                    <  2:2426660/149‑1 (MQ=255)
                     acaGCTGTAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGc                                                                                                                               <  2:2716854/149‑1 (MQ=255)
                     acaGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGc                                                                                                                               <  2:375718/149‑1 (MQ=255)
                     acaGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGc                                                                                                                               >  2:4164917/1‑149 (MQ=255)
                        gCTGGAAAAAAGCCTTAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGa                                                                                                                            <  2:3269302/149‑1 (MQ=255)
                           ggAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTg                                                                                                                         >  1:3658811/1‑149 (MQ=255)
                             aaaaaaGCCTTAAATCACCCCGCTGTGTCGCACCGTGGCCGCGCGTGGTGGGGCAAATAAAACTGGAGCGAGAGTATCAGTATATCGCTGCCCTGCCAAATAACGCGATTTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGcc                                                                                                                       <  1:2315135/149‑1 (MQ=255)
                              aaaaaGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGccc                                                                                                                      >  2:4413137/1‑149 (MQ=255)
                                 aaGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCcgcg                                                                                                                   >  2:4437098/1‑149 (MQ=255)
                                  aGCCTGAAATCCCCACGCAGTGGCGCCCCGTGGACGCGCGCGGAGTGGCAAAGAAACCTGGCGCGAGAGTATCAGGATATCGCGGCCCTGCCACAGACCGCGATGTTAAAAATCACACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGt                                                                                                                  <  1:3412875/149‑1 (MQ=255)
                                  aGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGt                                                                                                                  <  2:2534563/149‑1 (MQ=255)
                                   gCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTc                                                                                                                 >  2:2744044/1‑149 (MQ=255)
                                   gCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTc                                                                                                                 <  2:1103542/149‑1 (MQ=255)
                                    ccTGAAACCCCCACGCCGTGTCGCACCGGGGCCGCGCGAGGTGTGGCAAAGAAACCGGGCGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGATTTTAAAAACCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCt                                                                                                                <  1:1367438/149‑1 (MQ=255)
                                    catGAAATCACCACGCAGTGTCGCACCGTGTCCGGGCTAGGAGTGGCAAAGAAAACTGGAGGGAGTTTATCCGGATATCGCTGCCCTGCCAAAGAACGCGATGTAAAAAATAAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCt                                                                                                                <  2:1981131/147‑1 (MQ=255)
                                      tGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTgg                                                                                                              <  2:740297/149‑1 (MQ=255)
                                      tGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTgg                                                                                                              >  2:4316535/1‑149 (MQ=255)
                                            caccacGCAGTGTCGCACCGTGGACGCGCGAGGATTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGACCGCTAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACaaa                                                                                                        <  2:2198571/149‑1 (MQ=255)
                                            caccacGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACaaa                                                                                                        >  1:4323907/1‑149 (MQ=255)
                                            caccacGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACaaa                                                                                                        >  1:4323766/1‑149 (MQ=255)
                                             accacGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAg                                                                                                       >  2:3345540/1‑149 (MQ=255)
                                              ccacGCAGTGTCGCCCCGTGGACGCGCGAGGTGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCACACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAgg                                                                                                      <  1:1549633/149‑1 (MQ=255)
                                                acGCAGTGTCGCACCGTGGACGCGCGTGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCACACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGgag                                                                                                    <  1:2727352/149‑1 (MQ=255)
                                                 cGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGgaga                                                                                                   <  2:1470890/149‑1 (MQ=255)
                                                     gtgtCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCaa                                                                                               >  1:1489277/1‑149 (MQ=255)
                                                       gtCGCACCGTGGACGCGCGAGGAGTGGAAAAGAAAACTGGAGCGAGATTATCAGTATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCACACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCaaaa                                                                                             <  2:445372/149‑1 (MQ=255)
                                                        tCGCACCGTGGACGCGCGAGTTGTGGAAAATAAAACTGGCGCGTTATTATCTGTATATCGCTCCCCTGCCAAATAACGCTAATTTAAAAAACACACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCaaaaa                                                                                            <  1:1263860/149‑1 (MQ=255)
                                                         cGCCCCGTGGCCGCGCGTGGTGTGTCAAATAAAACTGGCGCGAGAGTATCTGTATAGCGCGGCCCTGCCACAGAACGCTAAGTTAAAAAACAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAAc                                                                                           <  1:453222/149‑1 (MQ=255)
                                                          gCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACa                                                                                          >  1:4431701/1‑149 (MQ=255)
                                                             cggTGGACGCGCGAGTTGGGGCAAATAAAAGTGGAGCGAGTGTATCTGGATATCGCTGCCCTGCCAATGACCGCTAATTTAAAAATCACACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGt                                                                                       <  1:186197/147‑1 (MQ=255)
                                                             ccGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGt                                                                                       >  1:3701657/1‑149 (MQ=255)
                                                             ccGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGt                                                                                       >  2:2340758/1‑149 (MQ=255)
                                                              cGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTc                                                                                      <  2:4039721/149‑1 (MQ=255)
                                                               gTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTcc                                                                                     >  1:815844/1‑149 (MQ=255)
                                                               gTGGACGCGCGAGGAGTGGAAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTcc                                                                                     <  2:2504355/149‑1 (MQ=255)
                                                                tGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCa                                                                                    >  2:3783077/1‑149 (MQ=255)
                                                                 ggACGCGGGTGTTGTGGAAAATAAAAGTGGAGCGTGTTTATCTGGATATCGCTGCCCTGCCACAGAACGCTATTTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCaa                                                                                   <  1:1405420/149‑1 (MQ=255)
                                                                        cgAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCt                                                                            <  2:1499620/149‑1 (MQ=255)
                                                                         gaggagTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTg                                                                           >  1:2832306/1‑149 (MQ=255)
                                                                              gTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAAACAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAATGAGATCAAAAACAAGTCCAACACGCCTGCCCTa                                                                      >  2:3067043/1‑149 (MQ=255)
                                                                                ggCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTaca                                                                    >  2:3889217/1‑149 (MQ=255)
                                                                                   aaaTAAAAGTGGCGCTAGATTATCGTTATATCGCTGCCCTGCAAAATAACGCTATTTAAAAAATAAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTAcacca                                                                 <  1:904084/149‑1 (MQ=255)
                                                                                    aaGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCATGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTAcaccac                                                                <  2:1011025/149‑1 (MQ=255)
                                                                                      gAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTg                                                              >  2:3515440/1‑149 (MQ=255)
                                                                                      gAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTg                                                              >  1:1744621/1‑149 (MQ=255)
                                                                                          aCTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTg                                                          >  2:417199/1‑149 (MQ=255)
                                                                                           cTGCAGCGAGAGTATCAGTATAGCGCGGCCCTGCACCAGACCGCTATTTTAAAAACCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGc                                                         <  1:3942815/149‑1 (MQ=255)
                                                                                             ggAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCAc                                                       >  1:3216797/1‑149 (MQ=255)
                                                                                                gCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGa                                                    <  2:2291059/149‑1 (MQ=255)
                                                                                                  gagaGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGAtt                                                  >  2:3744132/1‑149 (MQ=255)
                                                                                                      gTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATc                                              >  2:2828984/1‑149 (MQ=255)
                                                                                                      gTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATc                                              >  2:487157/1‑149 (MQ=255)
                                                                                                          cAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGa                                          >  2:478354/1‑149 (MQ=255)
                                                                                                          cAGGATAGCGCTGCCCTGCCACAGAACGCTAAGTTAAAAATAAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGa                                          <  1:2409736/149‑1 (MQ=255)
                                                                                                            ggATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATa                                        <  2:1554150/149‑1 (MQ=255)
                                                                                                             tatagCGCTGCCCTCCCACAGACCGCTAATTTAAAAACCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATaa                                       <  1:3559210/148‑1 (MQ=255)
                                                                                                               tatCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATg                                     <  2:1866364/149‑1 (MQ=255)
                                                                                                                atCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATgg                                    <  2:884266/149‑1 (MQ=255)
                                                                                                                atCGCTGCCCTGCCAAAGAACGGTATGTTAAAAATCACACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATgg                                    <  1:1011429/149‑1 (MQ=255)
                                                                                                                atCGCGGCCCTGCCACAGAACGCTAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATgg                                    <  1:3347521/149‑1 (MQ=255)
                                                                                                                 tCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATAACACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGgc                                   <  1:3883022/149‑1 (MQ=255)
                                                                                                                   gCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGgcgc                                 <  1:3843855/149‑1 (MQ=255)
                                                                                                                   gCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGgcgc                                 >  2:4509431/1‑149 (MQ=255)
                                                                                                                   gCTGCCCTGCCAAAGAACGCGAATTAAAAAAAACAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGgcgc                                 <  1:1312760/149‑1 (MQ=255)
                                                                                                                     tgccctgccACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCgg                               <  2:1663750/149‑1 (MQ=255)
                                                                                                                      gccctgccACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCGGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCggg                              >  2:4368362/1‑149 (MQ=255)
                                                                                                                        cctgccACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCg                            >  1:181727/1‑149 (MQ=255)
                                                                                                                        cctgccACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCg                            >  1:2248751/1‑149 (MQ=255)
                                                                                                                         cggccACAGACCGCGAAGTTAAAAATCACACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGt                           <  1:3829797/147‑1 (MQ=255)
                                                                                                                           gccACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGc                         >  1:1897255/1‑149 (MQ=255)
                                                                                                                           gccACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGc                         <  1:2325815/149‑1 (MQ=255)
                                                                                                                              acaGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCgg                      >  1:3798267/1‑149 (MQ=255)
                                                                                                                              acaGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCgg                      >  1:555100/1‑149 (MQ=255)
                                                                                                                               caGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGa                     >  1:1220922/1‑149 (MQ=255)
                                                                                                                               caGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGa                     <  2:1235668/149‑1 (MQ=255)
                                                                                                                                aGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGAc                    >  2:2077083/1‑149 (MQ=255)
                                                                                                                                  aaCGCTAAGTTAAAAATCACACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGt                  <  1:2417028/149‑1 (MQ=255)
                                                                                                                                  aaCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGt                  >  2:60285/1‑149 (MQ=255)
                                                                                                                                  aaCGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGt                  >  1:3146192/1‑149 (MQ=255)
                                                                                                                                     gcgAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTgg               <  2:3701657/149‑1 (MQ=255)
                                                                                                                                          gTTAAAAATCACACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAgt          <  1:3494523/149‑1 (MQ=255)
                                                                                                                                          gTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAgt          >  1:4143296/1‑149 (MQ=255)
                                                                                                                                             aaaaaTCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAgttgt       <  2:2768886/149‑1 (MQ=255)
                                                                                                                                              aaaaTCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAgttgtt      <  2:3292085/149‑1 (MQ=255)
                                                                                                                                               aaaTCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTa     >  2:1669591/1‑149 (MQ=255)
                                                                                                                                               aaaTCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTa     >  2:2772726/1‑149 (MQ=255)
                                                                                                                                                aaTCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACACGTCCAACACGCCTGCCCTACCCCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTaa    >  1:797904/1‑149 (MQ=255)
                                                                                                                                                aaTCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGGCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGAGAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTaa    >  1:1804565/1‑149 (MQ=255)
                                                                                                                                                 aTCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTaaa   <  2:3216797/149‑1 (MQ=255)
                                                                                                                                                  tCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAAt  >  1:3607689/1‑149 (MQ=255)
                                                                                                                                                  tCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAAt  >  2:4594334/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ACATCGTGATGAAGTCCTGGCACAGCTGGAAAAAAGCCTGAAATCACCACGCAGTGTCGCACCGTGGACGCGCGAGGAGTGGCAAAGAAAACTGGAGCGAGAGTATCAGGATATCGCTGCCCTGCCACAGAACGCGAAGTTAAAAATCAAACGTCCGGTGAAGGTGCAGCCGATTGCCCGCGTCTGGTACAAAGGAGATCAAAAACAAGTCCAACACGCCTGCCCTACACCACTGATTGCACTGATTAATCGGGATAATGGCGCGGGCGTGCCGGACGTTGGTGAGTTGTTAAAT  >  CP009273/1679058‑1679352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑