BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,136,421	A→G	20.7%	D3D (GAT→GAC)	udk ←	uridine/cytidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,136,421	0	A	G	20.7%	629.2 / 170.8	280	D3D (GAT→GAC)	udk	uridine/cytidine kinase
Reads supporting (aligned to +/- strand): ref base A (100/122); new base G (32/26); total (132/148)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 281 total aligned reads displayed.

ATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTC  >  CP009273/2136274‑2136569

aTCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGAt                                                                                                                                                     <  2:3814121/149‑1 (MQ=255)

       tAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCat                                                                                                                                              >  2:691903/1‑149 (MQ=255)

        aaTAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCata                                                                                                                                             <  1:1514753/149‑1 (MQ=255)

          tAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCatata                                                                                                                                           <  2:1368913/149‑1 (MQ=255)

            gCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATAtt                                                                                                                                         <  2:2877780/149‑1 (MQ=255)

              aGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTa                                                                                                                                       >  1:3932488/1‑149 (MQ=255)

               gTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAg                                                                                                                                      <  1:844439/149‑1 (MQ=255)

               gTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAg                                                                                                                                      <  2:3164282/149‑1 (MQ=255)

               gTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAg                                                                                                                                      <  2:3753182/149‑1 (MQ=255)

                    cGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGAcc                                                                                                                                 >  1:1133309/1‑149 (MQ=255)

                    cGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGAcc                                                                                                                                 <  2:52670/149‑1 (MQ=255)

                    cGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGAcc                                                                                                                                 <  1:394858/149‑1 (MQ=255)

                     gggAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCt                                                                                                                                >  2:1447014/1‑149 (MQ=255)

                          ttACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTa                                                                                                                           >  1:3786431/1‑149 (MQ=255)

                            aCGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAAc                                                                                                                         >  2:815238/1‑149 (MQ=255)

                            aCGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACGGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAAc                                                                                                                         <  1:2472911/149‑1 (MQ=255)

                             cGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAAcc                                                                                                                        <  1:2888801/149‑1 (MQ=255)

                              gCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCt                                                                                                                       <  1:2282421/149‑1 (MQ=255)

                              gCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCt                                                                                                                       <  1:771528/149‑1 (MQ=255)

                                agATGTGTTCATCACTGACTTGCTCAAGCAATTCTCGATATAGGGTACTGGAAATATTTCTCTTGCCGGAATCCGATTCGCCAGCGATACCTTTAATTACGCAATGATGTGACTGATCAGTAATATATTTAGCGACCTGATTAACCTgg                                                                                                                     <  1:3041486/148‑1 (MQ=255)

                                 gATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGa                                                                                                                    >  1:1874433/1‑149 (MQ=255)

                                 gATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGa                                                                                                                    <  1:4006277/149‑1 (MQ=255)

                                 gATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGa                                                                                                                    >  2:1583319/1‑149 (MQ=255)

                                     tgtTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGtt                                                                                                                <  2:4201216/149‑1 (MQ=255)

                                          atcaCCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGaa                                                                                                           >  2:457769/1‑149 (MQ=255)

                                          atcaCCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGaa                                                                                                           <  2:150404/149‑1 (MQ=255)

                                           tcaCCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAg                                                                                                          >  1:1374349/1‑149 (MQ=255)

                                             aCCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAggg                                                                                                        <  1:4242607/149‑1 (MQ=255)

                                              ccGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAgggg                                                                                                       >  2:3513845/1‑149 (MQ=255)

                                                    tGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGcgacg                                                                                                 >  2:2179697/1‑149 (MQ=255)

                                                      cTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGaa                                                                                               >  2:2849625/1‑149 (MQ=255)

                                                       tCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAg                                                                                              >  1:4390827/1‑149 (MQ=255)

                                                          cGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAgcgc                                                                                           <  1:4506626/149‑1 (MQ=255)

                                                            cAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCa                                                                                         >  1:623632/1‑149 (MQ=255)

                                                             aaTTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCaa                                                                                        >  1:3013560/1‑149 (MQ=255)

                                                              aTTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGGTACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCaaa                                                                                       >  2:2667423/1‑149 (MQ=255)

                                                              aTTCACGATAAAGGGTACTGGAAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCaaa                                                                                       <  1:3196496/149‑1 (MQ=255)

                                                                 cACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAAcgc                                                                                    <  1:3861447/149‑1 (MQ=255)

                                                                    gATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGc                                                                                 <  1:1287504/149‑1 (MQ=255)

                                                                    gATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGc                                                                                 >  2:1039543/1‑149 (MQ=255)

                                                                      tAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCaa                                                                               >  2:3370156/1‑149 (MQ=255)

                                                                       aaaGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAAt                                                                              >  2:2948290/1‑149 (MQ=255)

                                                                        aaGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAAtt                                                                             <  2:94979/149‑1 (MQ=255)

                                                                         aGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATta                                                                            >  1:3652658/1‑149 (MQ=255)

                                                                          gggTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATtat                                                                           <  1:3592488/149‑1 (MQ=255)

                                                                          gggTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATtat                                                                           <  1:1602125/149‑1 (MQ=255)

                                                                           ggTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATtata                                                                          <  1:2424957/149‑1 (MQ=255)

                                                                           ggTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATtata                                                                          <  1:2637042/149‑1 (MQ=255)

                                                                            gTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAg                                                                         <  1:4101980/149‑1 (MQ=255)

                                                                            gTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAg                                                                         >  1:1683029/1‑149 (MQ=255)

                                                                             tACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAgg                                                                        >  2:1883321/1‑149 (MQ=255)

                                                                               cTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGa                                                                      <  1:4051520/149‑1 (MQ=255)

                                                                               cTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGa                                                                      <  1:1671244/149‑1 (MQ=255)

                                                                                tGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGAt                                                                     >  1:4670728/1‑149 (MQ=255)

                                                                                  gCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGAttt                                                                   >  1:1743367/1‑149 (MQ=255)

                                                                                  gCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGAttt                                                                   >  2:4118935/1‑149 (MQ=255)

                                                                                      tAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATc                                                               <  2:244598/149‑1 (MQ=255)

                                                                                         gACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGt                                                            >  1:3836957/1‑149 (MQ=255)

                                                                                          aCTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTc                                                           >  2:1995690/1‑149 (MQ=255)

                                                                                          aCTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTc                                                           >  1:3493821/1‑149 (MQ=255)

                                                                                           ctctTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCt                                                          <  1:1159633/149‑1 (MQ=255)

                                                                                              ttGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGAt                                                       <  1:4341219/149‑1 (MQ=255)

                                                                                               tGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATa                                                      >  1:1952586/1‑149 (MQ=255)

                                                                                               tGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATa                                                      <  2:2323920/149‑1 (MQ=255)

                                                                                                  cGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCa                                                   >  2:3431819/1‑149 (MQ=255)

                                                                                                    gAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGt                                                 >  1:3260435/1‑149 (MQ=255)

                                                                                                    gAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGt                                                 >  2:3952105/1‑149 (MQ=255)

                                                                                                     aaGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTc                                                <  2:1272001/149‑1 (MQ=255)

                                                                                                        ccGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGaa                                             >  1:3766975/1‑149 (MQ=255)

                                                                                                        ccGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGCCGaa                                             <  1:2157747/149‑1 (MQ=255)

                                                                                                          gATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATa                                           >  2:2598646/1‑149 (MQ=255)

                                                                                                          gATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAAGTATAGGGATTTCATCCGTCTGATACCAGTCGAATa                                           >  2:3296138/1‑149 (MQ=255)

                                                                                                          gATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATa                                           <  1:106054/149‑1 (MQ=255)

                                                                                                           aTGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAg                                          >  2:176830/1‑149 (MQ=255)

                                                                                                            tGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGc                                         >  2:3267241/1‑149 (MQ=255)

                                                                                                              cgcCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGt                                       <  2:623632/149‑1 (MQ=255)

                                                                                                                 cAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTNc                                    <  2:4392121/149‑1 (MQ=255)

                                                                                                                 cAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGc                                    <  2:3450514/149‑1 (MQ=255)

                                                                                                                 cAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGc                                    <  2:1802592/149‑1 (MQ=255)

                                                                                                                   gCGATACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCcg                                  >  2:1168635/1‑149 (MQ=255)

                                                                                                                      aTACCGATAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCcgcgc                               >  1:2342169/1‑149 (MQ=255)

                                                                                                                             tAATGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCaga                        <  2:2307542/149‑1 (MQ=255)

                                                                                                                              aaTGACGCACTGATGAGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCagag                       >  1:3107104/1‑149 (MQ=255)

                                                                                                                                  aCGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTaa                   >  2:4456331/1‑149 (MQ=255)

                                                                                                                                   cGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAAt                  <  1:529824/149‑1 (MQ=255)

                                                                                                                                        tgatgaGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAAttgttg             >  1:4158320/1‑149 (MQ=255)

                                                                                                                                        tgatgaGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAAttgttg             >  1:1024/1‑149 (MQ=255)

                                                                                                                                         gatgaGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGa            >  2:2083266/1‑149 (MQ=255)

                                                                                                                                          atgaGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGAc           >  2:1579548/1‑149 (MQ=255)

                                                                                                                                           tgaGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACa          >  2:2072988/1‑149 (MQ=255)

                                                                                                                                           tgaGACTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACa          >  2:1463101/1‑149 (MQ=255)

                                                                                                                                           tgaGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACa          >  1:2203197/1‑149 (MQ=255)

                                                                                                                                            gagaCTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACaa         <  2:3759810/149‑1 (MQ=255)

                                                                                                                                             agaCTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACaaa        <  1:2886106/149‑1 (MQ=255)

                                                                                                                                              gaCTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAg       >  2:3856798/1‑149 (MQ=255)

                                                                                                                                              gaCTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAg       >  2:347828/1‑149 (MQ=255)

                                                                                                                                              gaCTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAg       <  1:691903/149‑1 (MQ=255)

                                                                                                                                               aCTGGTCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGa      <  2:1075415/149‑1 (MQ=255)

                                                                                                                                                 tGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAAt    <  2:4617082/149‑1 (MQ=255)

                                                                                                                                                  gATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAAtt   <  1:2251826/149‑1 (MQ=255)

ATCTTTGTAATAGCAGTCTTCGGGAATTACGCCGATGTGTTCATCACCGACTTGCTCACGCAATTCACGATAAAGGGTACTGGCAATAAGACTCTTGCCGGAAGCCGATGCGCCAGCGATACCGATAATGACGCACTGATGAGACTGATCAGTCATATATTTAGCGACCTGATTAACCTGGATGTTAGGAAGGGGGCGACGAAGCGCCAAACGCGGCAATTATAGGGATTTCATCCGTCTGATACCAGTCGAATAGCGTTGCCGCGCGCTCAGAGTTAATTGTTGACAAAGAATTC  >  CP009273/2136274‑2136569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`