Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,144,474 C→A 84.7% intergenic (‑8/‑192) yegI ← / → yegJ protein kinase‑related putative non‑specific DNA‑binding protein/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,144,4740CA84.7% 667.6 / 110.6 250intergenic (‑8/‑192)yegI/yegJprotein kinase‑related putative non‑specific DNA‑binding protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (26/13);  new base A (93/118);  total (119/131)
Fisher's exact test for biased strand distribution p-value = 1.38e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

GGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTC  >  CP009273/2144327‑2144619
                                                                                                                                                   |                                                                                                                                                 
    gcgTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCggg                                                                                                                                              <  2:358750/149‑1 (MQ=255)
      gtgtGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTa                                                                                                                                            >  1:2379698/1‑149 (MQ=255)
      gtgtGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTa                                                                                                                                            >  2:4062281/1‑149 (MQ=255)
       tgtgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAg                                                                                                                                           >  2:2275129/1‑149 (MQ=255)
       tgtgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAg                                                                                                                                           >  1:1504674/1‑149 (MQ=255)
        gtgATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGt                                                                                                                                          <  2:4434900/149‑1 (MQ=255)
          gATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCCCCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGtta                                                                                                                                        <  2:2749113/149‑1 (MQ=255)
          gATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGtta                                                                                                                                        <  2:2748984/149‑1 (MQ=255)
            tAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttata                                                                                                                                      >  1:4209427/1‑149 (MQ=255)
             aaaTCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttatat                                                                                                                                     <  2:2383939/149‑1 (MQ=255)
              aaTCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGttatatt                                                                                                                                    >  2:1959077/1‑149 (MQ=255)
                tCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGttatattat                                                                                                                                  >  2:183728/1‑149 (MQ=255)
                tCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGttatattat                                                                                                                                  <  2:2062933/149‑1 (MQ=255)
                  ttGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTtatattatat                                                                                                                                <  2:302591/149‑1 (MQ=255)
                   tGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTatattatatt                                                                                                                               >  2:506463/1‑149 (MQ=255)
                       gACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTATCGGGTAGTTATATTATATTTCaa                                                                                                                           >  1:2668400/1‑149 (MQ=255)
                        aCGCTATCGACAAACTCCTCGATATCATAACCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAAt                                                                                                                          <  1:1916380/149‑1 (MQ=255)
                        aCGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAAt                                                                                                                          >  2:1561898/1‑149 (MQ=255)
                            tATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGaat                                                                                                                      >  1:2898956/1‑149 (MQ=255)
                                 aCAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAAtaataa                                                                                                                 >  1:1369941/1‑149 (MQ=255)
                                     aCTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATaacaac                                                                                                             <  1:4375832/149‑1 (MQ=255)
                                     aCTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATaacaac                                                                                                             >  1:4002559/1‑149 (MQ=255)
                                      ctcctcGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACt                                                                                                            <  2:1876573/149‑1 (MQ=255)
                                        cctcGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTaa                                                                                                          <  1:1348468/149‑1 (MQ=255)
                                        cctcGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTaa                                                                                                          <  1:3739300/149‑1 (MQ=255)
                                          tcGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAAcc                                                                                                        >  1:318349/1‑149 (MQ=255)
                                          tcGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAAcc                                                                                                        >  1:318359/1‑149 (MQ=255)
                                          tcGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAAcc                                                                                                        >  1:2818863/1‑149 (MQ=255)
                                            gATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAt                                                                                                      >  1:1093803/1‑149 (MQ=255)
                                             atatCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAtt                                                                                                     <  1:2449150/149‑1 (MQ=255)
                                             atatCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCAtt                                                                                                     >  2:4048565/1‑149 (MQ=255)
                                              tatCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTa                                                                                                    >  2:84435/1‑149 (MQ=255)
                                              tatCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTa                                                                                                    <  2:3203054/149‑1 (MQ=255)
                                               atCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTaa                                                                                                   >  1:2272482/1‑149 (MQ=255)
                                                tCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTaaa                                                                                                  <  2:644469/149‑1 (MQ=255)
                                                tCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTaaa                                                                                                  >  1:1703424/1‑149 (MQ=255)
                                                tCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTaaa                                                                                                  >  1:3629563/1‑149 (MQ=255)
                                                  aTAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAat                                                                                                <  1:1435045/149‑1 (MQ=255)
                                                  aTAAACCGCGCCTTCACCACCATTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAat                                                                                                <  1:4122031/149‑1 (MQ=255)
                                                   tAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAatt                                                                                               <  1:2740626/149‑1 (MQ=255)
                                                   tAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAatt                                                                                               >  2:1782420/1‑149 (MQ=255)
                                                     aaCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAattat                                                                                             <  1:607645/149‑1 (MQ=255)
                                                     aaCCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAattat                                                                                             <  1:1213222/149‑1 (MQ=255)
                                                          cgcCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCta                                                                                        <  2:125451/149‑1 (MQ=255)
                                                           gcCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCtat                                                                                       >  2:815540/1‑149 (MQ=255)
                                                           gcCGTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCtat                                                                                       <  1:3088459/149‑1 (MQ=255)
                                                              ttCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATg                                                                                    >  2:1691544/1‑149 (MQ=255)
                                                              ttCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATg                                                                                    >  2:4026441/1‑149 (MQ=255)
                                                                caccacCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATggg                                                                                  >  2:2325253/1‑149 (MQ=255)
                                                                 accaccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATgggg                                                                                 >  2:248915/1‑149 (MQ=255)
                                                                 accaccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATgggg                                                                                 >  2:2589749/1‑149 (MQ=255)
                                                                  ccaccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGa                                                                                <  2:45638/149‑1 (MQ=255)
                                                                  ccaccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGa                                                                                >  1:2372884/1‑149 (MQ=255)
                                                                   caccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATAATTTTATATTTGTTTTCATAATATTTATCGGGTAGTTATATTATATTTCAATGAATAATAACAAATAACCATTAAAATTATTTCTATATTGGGaa                                                                               >  1:3938495/1‑149 (MQ=255)
                                                                     ccTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGaaaa                                                                             >  1:1094872/1‑149 (MQ=255)
                                                                      cTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAAc                                                                            <  1:719898/149‑1 (MQ=255)
                                                                       tttGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAAcc                                                                           >  1:2134579/1‑149 (MQ=255)
                                                                          gCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCAc                                                                        <  1:4239402/149‑1 (MQ=255)
                                                                           cccAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACt                                                                       <  1:3557722/149‑1 (MQ=255)
                                                                             cAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCa                                                                     >  2:847833/1‑149 (MQ=255)
                                                                                 tCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGaa                                                                 <  1:414118/149‑1 (MQ=255)
                                                                                   aCGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATa                                                               >  1:927164/1‑149 (MQ=255)
                                                                                    cGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATaa                                                              <  1:3024314/149‑1 (MQ=255)
                                                                                    cGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATaa                                                              >  1:2471784/1‑149 (MQ=255)
                                                                                     ggCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATaaa                                                             <  2:3064942/149‑1 (MQ=255)
                                                                                       ccAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATTGGGAAAACCCACTCAAGAATaaaaa                                                           >  2:4462251/1‑149 (MQ=255)
                                                                                          agagTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGa                                                        <  1:3161823/149‑1 (MQ=255)
                                                                                           gagTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGAt                                                       <  1:1381468/149‑1 (MQ=255)
                                                                                            agTGGTCAATTCACCAGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATa                                                      <  1:4170486/149‑1 (MQ=255)
                                                                                               ggTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATg                                                   >  1:2872105/1‑149 (MQ=255)
                                                                                                gTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGc                                                  >  2:3962279/1‑149 (MQ=255)
                                                                                                gTCAATTCACCTGTCGATGTAAATACTTTTATATATGTTTTCATAATATTTAGCGGGTAGTTATATAAAATTTCAATGAATAATAACAACTAACCATTAAAATTATTTATATATGGGGAAAACCCACTCAAGAATACAAATGATAATGc                                                  >  2:1628533/1‑149 (MQ=255)
                                                                                                  cAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCaa                                                <  2:3417150/149‑1 (MQ=255)
                                                                                                    aTTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAAtt                                              <  2:3525619/149‑1 (MQ=255)
                                                                                                     ttCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAAttt                                             <  2:4516463/149‑1 (MQ=255)
                                                                                                        aCCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTAt                                          <  2:2048106/149‑1 (MQ=255)
                                                                                                        aCCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTAt                                          >  2:4194897/1‑149 (MQ=255)
                                                                                                           tGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAg                                       >  1:3126258/1‑149 (MQ=255)
                                                                                                           tGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAg                                       <  1:1052569/149‑1 (MQ=255)
                                                                                                           tGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAACAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAg                                       >  2:715707/1‑149 (MQ=255)
                                                                                                            gTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAgg                                      >  2:1751575/1‑149 (MQ=255)
                                                                                                             tCGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGa                                     <  2:1030236/149‑1 (MQ=255)
                                                                                                              cGATGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGAt                                    <  2:1081533/149‑1 (MQ=255)
                                                                                                                aTGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAc                                  >  1:2722535/1‑149 (MQ=255)
                                                                                                                 tGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAca                                 >  1:1648253/1‑149 (MQ=255)
                                                                                                                 tGTAAATACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAca                                 <  1:2648617/149‑1 (MQ=255)
                                                                                                                       tACTTTTATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATAcacttcaa                           >  2:2979123/1‑149 (MQ=255)
                                                                                                                            ttATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCg                      <  1:1324102/149‑1 (MQ=255)
                                                                                                                            ttATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCg                      >  2:3342174/1‑149 (MQ=255)
                                                                                                                            ttATATTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCg                      <  1:1324261/149‑1 (MQ=255)
                                                                                                                                atTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCt                  <  2:4251176/149‑1 (MQ=255)
                                                                                                                                atTTGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATCTTATCAGGATACAATTCAATTTCGGCCt                  >  2:2022750/1‑149 (MQ=255)
                                                                                                                                 tttGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTg                 >  1:654602/1‑149 (MQ=255)
                                                                                                                                 tttGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTg                 >  1:441976/1‑149 (MQ=255)
                                                                                                                                 tttGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTg                 >  1:4037641/1‑149 (MQ=255)
                                                                                                                                   tGTTTTCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACACTTCAATTTCGGCCTGAt               <  1:3365241/149‑1 (MQ=255)
                                                                                                                                    gTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATa              >  2:3129327/1‑149 (MQ=255)
                                                                                                                                      tttCATAATATTTAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAAt            >  1:1739143/1‑149 (MQ=255)
                                                                                                                                            aataTTTAGCGGGTAGTTATATTATATTTCAATGAAAAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTaa      >  1:4638331/1‑149 (MQ=255)
                                                                                                                                                tttAGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTc  <  1:233953/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGCGGCGTGTGATAAATCTTGGCGACGCTATCGACAAACTCCTCGATATCATAAACCGCGCCTTCACCACCTTTGCCCAGTTCACGGCCAAGAGTGGTCAATTCACCTGTCGATGTAAATACTTTTATATTTGTTTTCATAATATTTCGCGGGTAGTTATATTATATTTCAATGAATAATAACAACTAACCATTAAAATTATTTCTATATGGGGAAAACCCACTCAAGAATAAAAATGATAATGCAATTTTATCAGGATACAATTCAATTTCGGCCTGATAATGATTAACTTC  >  CP009273/2144327‑2144619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑