BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,289,374	C→A	81.2%	V142V (GTG→GTT)	ccmC ←	heme exporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,289,374	0	C	A	81.2%	711.3 / 197.0	314	V142V (GTG→GTT)	ccmC	heme exporter subunit
Reads supporting (aligned to +/- strand): ref base C (29/30); new base A (127/128); total (156/158)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 316 total aligned reads displayed.

TGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCATCGCCG  >  CP009273/2289228‑2289519

tgcATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTACTGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATaac                                                                                                                                                 >  1:830286/1‑149 (MQ=255)

    tCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACAccc                                                                                                                                             <  2:3459083/149‑1 (MQ=255)

    tCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACAccc                                                                                                                                             <  1:883982/149‑1 (MQ=255)

       gcgTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCcaca                                                                                                                                          <  1:2992891/149‑1 (MQ=255)

        cgTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACAt                                                                                                                                         <  1:1750869/149‑1 (MQ=255)

         gTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAGCACCCACAtc                                                                                                                                        >  1:279811/1‑148 (MQ=255)

          ttGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATAc                                                                                                                                       >  1:2419419/1‑149 (MQ=255)

              tCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACaaaa                                                                                                                                   >  2:967502/1‑149 (MQ=255)

               cccTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACaaaaa                                                                                                                                  <  2:1735679/149‑1 (MQ=255)

               cccTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACaaaaa                                                                                                                                  <  2:4120746/149‑1 (MQ=255)

                ccTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAAc                                                                                                                                 >  1:3021194/1‑149 (MQ=255)

                   gATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAAcagc                                                                                                                              >  2:2752171/1‑149 (MQ=255)

                       cAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAgcagca                                                                                                                          <  1:3420140/149‑1 (MQ=255)

                        aGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCAc                                                                                                                         >  2:117424/1‑149 (MQ=255)

                        aGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCAc                                                                                                                         >  1:917257/1‑149 (MQ=255)

                            tgtTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGt                                                                                                                     <  2:734804/149‑1 (MQ=255)

                             gtTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGtt                                                                                                                    <  2:3393066/149‑1 (MQ=255)

                             gtTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGtt                                                                                                                    >  1:4474587/1‑149 (MQ=255)

                               tCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCa                                                                                                                  <  2:493291/149‑1 (MQ=255)

                                 caccacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGa                                                                                                                >  2:4053650/1‑149 (MQ=255)

                                  accacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGaa                                                                                                               <  2:3427514/149‑1 (MQ=255)

                                    cacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGt                                                                                                             >  2:4069534/1‑149 (MQ=255)

                                    cacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGt                                                                                                             <  2:2277424/149‑1 (MQ=255)

                                    cacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGt                                                                                                             >  2:2627905/1‑149 (MQ=255)

                                    cacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGt                                                                                                             <  2:2468220/149‑1 (MQ=255)

                                    cacTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGt                                                                                                             <  1:2229450/149‑1 (MQ=255)

                                     acTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTc                                                                                                            >  2:4186790/1‑149 (MQ=255)

                                     acTCCACGGAGTAATGAATAATAGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTc                                                                                                            >  1:3154292/1‑149 (MQ=255)

                                      cTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCa                                                                                                           <  1:4362118/149‑1 (MQ=255)

                                           ctgAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGTATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGACGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCTCCTGTTCAGAAGTCAGACGt                                                                                                      <  2:4426630/147‑1 (MQ=255)

                                              aGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCa                                                                                                   <  2:3715111/149‑1 (MQ=255)

                                               gTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCAt                                                                                                  >  2:1012830/1‑149 (MQ=255)

                                                    gAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCAt                                                                                             <  1:598133/149‑1 (MQ=255)

                                                      ataatCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATAc                                                                                           <  2:144655/149‑1 (MQ=255)

                                                       taatCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCAACATACAAAAAAAGCAGCACCAGTTCAGAAGTCAGACGTGCatcccatccc                                                                                          >  2:591414/1‑149 (MQ=255)

                                                       taatCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGACACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATAcc                                                                                          <  2:2393654/149‑1 (MQ=255)

                                                         atCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACcca                                                                                        >  2:4518428/1‑149 (MQ=255)

                                                          tCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACccac                                                                                       <  2:794740/149‑1 (MQ=255)

                                                            ggCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACccacca                                                                                     >  1:4503047/1‑149 (MQ=255)

                                                             gCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAg                                                                                    <  1:4126458/149‑1 (MQ=255)

                                                              cAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAgg                                                                                   <  1:3269592/149‑1 (MQ=255)

                                                                gATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTg                                                                                 >  1:743286/1‑149 (MQ=255)

                                                                    caccacGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGcccc                                                                             >  1:3183219/1‑149 (MQ=255)

                                                                      ccacGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCATACGTCTGTCGTCGAAGGCGTCCCACAGGGAAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACTTGCAACACATACCCACCAGGTGCCCcac                                                                           >  1:2333894/1‑149 (MQ=255)

                                                                      ccacGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCcac                                                                           >  2:2999727/1‑149 (MQ=255)

                                                                      ccacGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCcac                                                                           >  2:3339805/1‑149 (MQ=255)

                                                                      ccacGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCcac                                                                           >  2:2924575/1‑149 (MQ=255)

                                                                       cacGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCcaca                                                                          <  2:4571178/149‑1 (MQ=255)

                                                                       cacGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCcaca                                                                          >  1:621404/1‑149 (MQ=255)

                                                                           ccAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCgg                                                                      <  1:198183/149‑1 (MQ=255)

                                                                            cAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGt                                                                     >  1:2197682/1‑149 (MQ=255)

                                                                             aaTCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGtt                                                                    <  2:2165738/149‑1 (MQ=255)

                                                                             aaTCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGtt                                                                    >  2:1824047/1‑149 (MQ=255)

                                                                              aTCAGCACCCGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGttt                                                                   <  1:137158/149‑1 (MQ=255)

                                                                              aTCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGttt                                                                   >  1:3536551/1‑149 (MQ=255)

                                                                               tCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGtttt                                                                  >  2:463255/1‑149 (MQ=255)

                                                                                   cACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTcccc                                                              >  1:2965548/1‑149 (MQ=255)

                                                                                     ccAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCAt                                                            >  2:256029/1‑149 (MQ=255)

                                                                                      cAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATg                                                           >  2:3765227/1‑149 (MQ=255)

                                                                                       aGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGc                                                          >  2:435452/1‑149 (MQ=255)

                                                                                        ggATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCa                                                         <  1:4639349/149‑1 (MQ=255)

                                                                                             ccTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGcc                                                    <  1:3266786/149‑1 (MQ=255)

                                                                                             ccTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGcc                                                    <  1:1588586/149‑1 (MQ=255)

                                                                                                gCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGTAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGt                                                 <  1:2593076/149‑1 (MQ=255)

                                                                                                gCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGt                                                 <  1:2530676/149‑1 (MQ=255)

                                                                                                    cACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAAcc                                             >  2:1998993/1‑149 (MQ=255)

                                                                                                     aCGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCa                                            >  1:530882/1‑149 (MQ=255)

                                                                                                      cGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAg                                           <  2:153070/149‑1 (MQ=255)

                                                                                                      cGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAg                                           <  1:2294750/149‑1 (MQ=255)

                                                                                                           cGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCaa                                      <  1:3341343/149‑1 (MQ=255)

                                                                                                             ccAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATa                                    <  2:3548056/149‑1 (MQ=255)

                                                                                                             ccAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATa                                    <  1:2752171/149‑1 (MQ=255)

                                                                                                               aGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATaaa                                  >  2:1186336/1‑149 (MQ=255)

                                                                                                                gACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAg                                 <  1:1028778/149‑1 (MQ=255)

                                                                                                                   ggcggTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTa                              <  1:4393986/149‑1 (MQ=255)

                                                                                                                    gcggTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTaa                             >  2:3116480/1‑149 (MQ=255)

                                                                                                                    gcggTCGTCGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCAGACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTaa                             <  1:2877498/149‑1 (MQ=255)

                                                                                                                     cggTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTaaa                            <  1:3659643/149‑1 (MQ=255)

                                                                                                                      ggTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAac                           <  1:332808/149‑1 (MQ=255)

                                                                                                                       gtcgtcGAAGGCGTGCCACAGGGCAATAGCACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAaca                          <  2:279811/149‑1 (MQ=255)

                                                                                                                       gtcgtcGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAaca                          <  1:1865130/149‑1 (MQ=255)

                                                                                                                       gtcgtcGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAaca                          >  1:1174922/1‑149 (MQ=255)

                                                                                                                       gtcgtcGAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAaca                          >  1:3807887/1‑149 (MQ=255)

                                                                                                                           tcgAAGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAGGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGc                      >  1:1623186/1‑149 (MQ=255)

                                                                                                                              aaGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCAcc                   >  2:2350713/1‑149 (MQ=255)

                                                                                                                              aaGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCAcc                   >  2:2037304/1‑149 (MQ=255)

                                                                                                                              aaGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCAcc                   <  1:327279/149‑1 (MQ=255)

                                                                                                                               aGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCa                  >  2:3678892/1‑149 (MQ=255)

                                                                                                                               aGGCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCa                  >  1:3852739/1‑149 (MQ=255)

                                                                                                                                ggCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCaa                 <  2:3067565/149‑1 (MQ=255)

                                                                                                                                ggCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCaa                 <  1:2489172/149‑1 (MQ=255)

                                                                                                                                ggCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCaa                 <  2:2179833/149‑1 (MQ=255)

                                                                                                                                ggCGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCaa                 <  1:3999581/149‑1 (MQ=255)

                                                                                                                                  cGTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATg               <  1:49736/149‑1 (MQ=255)

                                                                                                                                   gTGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATgg              <  1:2687215/149‑1 (MQ=255)

                                                                                                                                    tGCCACAGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATggg             <  1:4235276/149‑1 (MQ=255)

                                                                                                                                      ccACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATggggg           >  1:1492083/1‑149 (MQ=255)

                                                                                                                                       cacaGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGc          >  2:4544474/1‑149 (MQ=255)

                                                                                                                                       cacaGGGCAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGc          <  1:4089748/149‑1 (MQ=255)

                                                                                                                                              cAATAACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCATcgcc   <  1:2653992/149‑1 (MQ=255)

TGCATCCGCGTTGATCCCTGATGCAGGGTGTTCCACCACTCCACGGAGTAATGAATAATCGGCAGATTCACCACGCCAATCAGCACCAGGATACCTGCCGCACGGCCCGCCAGACGGCGGTCGTCGAAGGCGTGCCACAGGGCAATCACACCCACATACAAAAACAGCAGCACCAGTTCAGAAGTCAGACGTGCATCCCATACCCACCAGGTGCCCCACATCGGTTTTCCCCATGCAGAGCCGGTAACCAGGGCAATAAAGGTAAACACGGCACCAATGGGGGCCATCGCCG  >  CP009273/2289228‑2289519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`