Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,359,673 G→T 82.9% M89I (ATG→ATT)  arnB → uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,359,6730GT82.9% 640.7 / 138.5 261M89I (ATG→ATT) arnBuridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (23/22);  new base T (113/103);  total (136/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

AGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGATATTG  >  CP009273/2359526‑2359818
                                                                                                                                                   |                                                                                                                                                 
     tttGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTcc                                                                                                                                             <  2:422428/149‑1 (MQ=255)
      ttGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCt                                                                                                                                            <  1:1211163/149‑1 (MQ=255)
      ttGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCt                                                                                                                                            <  2:182597/149‑1 (MQ=255)
      ttGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCt                                                                                                                                            <  2:3213592/149‑1 (MQ=255)
       tGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCtt                                                                                                                                           <  1:1799592/149‑1 (MQ=255)
       tGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCtt                                                                                                                                           <  1:3970558/149‑1 (MQ=255)
           aGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCttgttg                                                                                                                                       >  2:59769/1‑149 (MQ=255)
            gTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTgg                                                                                                                                      >  1:4352709/1‑149 (MQ=255)
               gACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTg                                                                                                                                   <  2:4423001/149‑1 (MQ=255)
                aCGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGc                                                                                                                                  >  1:4167185/1‑149 (MQ=255)
                 cGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCa                                                                                                                                 >  1:279020/1‑149 (MQ=255)
                   ggAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAAc                                                                                                                               >  2:3699703/1‑149 (MQ=255)
                   ggAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAAc                                                                                                                               >  2:3699677/1‑149 (MQ=255)
                      aaTCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGcc                                                                                                                            >  1:3196350/1‑149 (MQ=255)
                          aGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTa                                                                                                                        >  1:2953492/1‑149 (MQ=255)
                          aGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTa                                                                                                                        <  1:806606/149‑1 (MQ=255)
                          aGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTa                                                                                                                        <  2:4338880/149‑1 (MQ=255)
                          aGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTa                                                                                                                        >  1:1718521/1‑149 (MQ=255)
                             aTGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAAtg                                                                                                                     >  2:4270818/1‑149 (MQ=255)
                             aTGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAAtg                                                                                                                     <  2:117859/149‑1 (MQ=255)
                              tGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAAtgg                                                                                                                    <  1:3900980/149‑1 (MQ=255)
                                  aTCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGa                                                                                                                >  2:3533176/1‑149 (MQ=255)
                                   tCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGAt                                                                                                               >  2:3803336/1‑149 (MQ=255)
                                     gcgGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGt                                                                                                             <  2:3046631/149‑1 (MQ=255)
                                        gTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGa                                                                                                          >  1:4605103/1‑149 (MQ=255)
                                        gTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGa                                                                                                          >  1:2263950/1‑149 (MQ=255)
                                         tCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGAc                                                                                                         <  2:2158354/149‑1 (MQ=255)
                                          cAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGAcc                                                                                                        <  1:3591583/149‑1 (MQ=255)
                                           aGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACcg                                                                                                       >  1:2752692/1‑149 (MQ=255)
                                               cAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGAt                                                                                                   <  2:204600/149‑1 (MQ=255)
                                               cAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGAt                                                                                                   <  2:2588006/149‑1 (MQ=255)
                                                aGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATa                                                                                                  <  1:1414355/149‑1 (MQ=255)
                                                 gCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATAc                                                                                                 >  2:3047931/1‑149 (MQ=255)
                                                 gCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATAc                                                                                                 <  1:497942/149‑1 (MQ=255)
                                                 gCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATAc                                                                                                 >  2:3855535/1‑149 (MQ=255)
                                                 gCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAAGATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATAc                                                                                                 >  2:2758347/1‑149 (MQ=255)
                                                    aCCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCt                                                                                              >  2:1020979/1‑149 (MQ=255)
                                                     ccgccgGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTg                                                                                             <  2:1362735/149‑1 (MQ=255)
                                                         cgGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATgg                                                                                         >  2:2358463/1‑149 (MQ=255)
                                                         cgGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATgg                                                                                         <  1:3171041/149‑1 (MQ=255)
                                                             aTGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCAc                                                                                     >  2:2998960/1‑149 (MQ=255)
                                                                 atatCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCt                                                                                 <  1:2307794/149‑1 (MQ=255)
                                                                  tatCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTg                                                                                >  1:1044511/1‑149 (MQ=255)
                                                                  tatCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTg                                                                                >  1:3379957/1‑149 (MQ=255)
                                                                     cACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAg                                                                             >  2:56237/1‑149 (MQ=255)
                                                                          tAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATc                                                                        <  1:4533625/149‑1 (MQ=255)
                                                                           aaTGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCg                                                                       >  1:5607/1‑149 (MQ=255)
                                                                               gCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTTATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTc                                                                   <  2:2031630/149‑1 (MQ=255)
                                                                               gCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTc                                                                   <  1:154422/149‑1 (MQ=255)
                                                                               gCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTc                                                                   <  1:3957306/149‑1 (MQ=255)
                                                                                 gTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAg                                                                 >  1:439553/1‑149 (MQ=255)
                                                                                 gTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAg                                                                 >  2:4346430/1‑149 (MQ=255)
                                                                                  ttGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGc                                                                >  2:1281152/1‑149 (MQ=255)
                                                                                  ttGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGc                                                                >  2:1676339/1‑149 (MQ=255)
                                                                                      aaaTTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCAtt                                                            >  2:2819103/1‑149 (MQ=255)
                                                                                       aaTTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTa                                                           <  2:1200825/149‑1 (MQ=255)
                                                                                              aaGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACg                                                    >  1:2600158/1‑149 (MQ=255)
                                                                                               aGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGc                                                   >  2:1024507/1‑149 (MQ=255)
                                                                                                gggCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCa                                                  >  1:853384/1‑149 (MQ=255)
                                                                                                 ggCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCAc                                                 <  1:2459314/149‑1 (MQ=255)
                                                                                                     aTGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTaaa                                             >  1:2644743/1‑149 (MQ=255)
                                                                                                     aTGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTaaa                                             >  1:2009183/1‑149 (MQ=255)
                                                                                                     aTGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTaaa                                             >  2:4106587/1‑149 (MQ=255)
                                                                                                       gAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGc                                           <  1:4023843/149‑1 (MQ=255)
                                                                                                         aGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCca                                         <  1:2215367/149‑1 (MQ=255)
                                                                                                         aGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCca                                         >  2:1737509/1‑149 (MQ=255)
                                                                                                            gATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCcatca                                      <  1:979104/149‑1 (MQ=255)
                                                                                                             aTTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCcatcat                                     >  1:2736214/1‑149 (MQ=255)
                                                                                                             aTTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCcatcat                                     >  2:2923108/1‑149 (MQ=255)
                                                                                                              ttACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCAtt                                    >  1:167475/1‑149 (MQ=255)
                                                                                                              ttACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCAtt                                    <  1:1665345/149‑1 (MQ=255)
                                                                                                               tACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTc                                   <  2:481177/149‑1 (MQ=255)
                                                                                                                aCGCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTcc                                  >  2:2515888/1‑149 (MQ=255)
                                                                                                                  gCCTTCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCgg                                <  1:2943418/149‑1 (MQ=255)
                                                                                                                  gCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCgg                                >  1:2870619/1‑149 (MQ=255)
                                                                                                                     ttCCCTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGc                             >  2:3996877/1‑149 (MQ=255)
                                                                                                                      tCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCa                            >  1:4258899/1‑149 (MQ=255)
                                                                                                                       cccTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCAt                           >  2:2916480/1‑149 (MQ=255)
                                                                                                                       cccTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCAt                           >  2:2284778/1‑149 (MQ=255)
                                                                                                                       cccTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCAt                           >  1:586988/1‑149 (MQ=255)
                                                                                                                        ccTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCAtt                          <  2:848080/149‑1 (MQ=255)
                                                                                                                         cTGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTa                         <  1:3765142/149‑1 (MQ=255)
                                                                                                                          tGACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTAt                        <  2:218275/149‑1 (MQ=255)
                                                                                                                           gACCTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATg                       >  2:1525535/1‑149 (MQ=255)
                                                                                                                            aCCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGc                      >  2:2876856/1‑149 (MQ=255)
                                                                                                                             ccTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGcc                     >  1:3651366/1‑149 (MQ=255)
                                                                                                                              cTGGGTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCg                    >  2:4622888/1‑149 (MQ=255)
                                                                                                                                  gTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTgc                >  2:1997107/1‑149 (MQ=255)
                                                                                                                                  gTTTCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTgc                <  2:1302801/149‑1 (MQ=255)
                                                                                                                                   tttCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTgcg               >  2:915044/1‑149 (MQ=255)
                                                                                                                                     tCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGcc             >  1:705002/1‑149 (MQ=255)
                                                                                                                                     tCAACCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGcc             <  1:3699677/149‑1 (MQ=255)
                                                                                                                                       aaCCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCcag           >  2:3386967/1‑149 (MQ=255)
                                                                                                                                        aCCCTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCcagc          >  1:3320573/1‑149 (MQ=255)
                                                                                                                                         cccTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCcagca         >  2:3646975/1‑149 (MQ=255)
                                                                                                                                         cccTCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCcagca         >  1:3704219/1‑149 (MQ=255)
                                                                                                                                          ccTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCcagcag        >  1:1142474/1‑149 (MQ=255)
                                                                                                                                           cTCAATATTATTTCCGTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGa       <  2:2435148/149‑1 (MQ=255)
                                                                                                                                            tCAATATTATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGat      <  1:3095554/149‑1 (MQ=255)
                                                                                                                                                tattatTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGATATTg  >  1:4507928/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTCAGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGATGAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGTGCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATCGAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCAGCAGATATTG  >  CP009273/2359526‑2359818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑