Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,390,862 C→A 12.9% V20V (GTG→GTT)  nuoH ← NADH:ubiquinone oxidoreductase, membrane subunit H

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,390,8620CA12.9% 908.2 / 85.9 264V20V (GTG→GTT) nuoHNADH:ubiquinone oxidoreductase, membrane subunit H
Reads supporting (aligned to +/- strand):  ref base C (123/107);  new base A (16/18);  total (139/125)
Fisher's exact test for biased strand distribution p-value = 5.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 281 total aligned reads displayed.

TAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGC  >  CP009273/2390714‑2391010
                                                                                                                                                    |                                                                                                                                                    
  aaGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcac                                                                                                                                                    >  1:632015/1‑149 (MQ=255)
   aGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacc                                                                                                                                                   >  2:1509520/1‑149 (MQ=255)
    gAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcacca                                                                                                                                                  <  1:728781/149‑1 (MQ=255)
    gAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATaacca                                                                                                                                                  >  1:3151244/1‑149 (MQ=255)
     aaCATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATcaccac                                                                                                                                                 <  1:4479835/149‑1 (MQ=255)
      aCATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCaccacc                                                                                                                                                >  2:703767/1‑149 (MQ=255)
      aCATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCaccacc                                                                                                                                                >  1:2798329/1‑149 (MQ=255)
       cATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCg                                                                                                                                               >  1:3204538/1‑149 (MQ=255)
             gATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGa                                                                                                                                         >  1:3030721/1‑149 (MQ=255)
              aTCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAg                                                                                                                                        <  1:3573559/149‑1 (MQ=255)
               tCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAgg                                                                                                                                       >  2:273280/1‑149 (MQ=255)
               tCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAgg                                                                                                                                       >  1:3347380/1‑149 (MQ=255)
                cATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGa                                                                                                                                      <  1:3653447/149‑1 (MQ=255)
                 aTGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGAt                                                                                                                                     >  1:2519555/1‑149 (MQ=255)
                  tGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATg                                                                                                                                    >  1:3884521/1‑149 (MQ=255)
                    tCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGt                                                                                                                                  >  2:373154/1‑149 (MQ=255)
                       gCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTcag                                                                                                                               >  1:1606338/1‑149 (MQ=255)
                         aaCCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACACCCACCCGCAGGATCACCACCGCTGTGAGGATGGTcagca                                                                                                                             >  2:3069944/1‑149 (MQ=255)
                          aCCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTcagcag                                                                                                                            >  2:2666662/1‑149 (MQ=255)
                           ccAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAgg                                                                                                                           >  1:400670/1‑149 (MQ=255)
                             aGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGAt                                                                                                                         >  1:1164723/1‑149 (MQ=255)
                              gCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACACCCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGAtc                                                                                                                        <  2:2203117/149‑1 (MQ=255)
                              gCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGAtc                                                                                                                        <  2:1400707/149‑1 (MQ=255)
                              gCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGAtc                                                                                                                        <  1:1382195/149‑1 (MQ=255)
                              gCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGAtc                                                                                                                        >  2:4105390/1‑149 (MQ=255)
                                 ggAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCa                                                                                                                     >  2:1899423/1‑149 (MQ=255)
                                 ggAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATAACCACCGCTTTGAGGATGGTCAGCAGGATCTCa                                                                                                                     >  2:19529/1‑149 (MQ=255)
                                   aGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAAt                                                                                                                   >  1:3178269/1‑149 (MQ=255)
                                   aGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATAACCACCGCTTTGAGGATGGTCAGCAGGATCTCAAt                                                                                                                   >  1:1240778/1‑149 (MQ=255)
                                       aaCCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGt                                                                                                               >  2:4395370/1‑149 (MQ=255)
                                       aaCCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGt                                                                                                               <  1:4621596/149‑1 (MQ=255)
                                        aCCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGtt                                                                                                              >  1:3928257/1‑149 (MQ=255)
                                         ccGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTc                                                                                                             >  2:416775/1‑149 (MQ=255)
                                         ccGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTc                                                                                                             <  1:705784/149‑1 (MQ=255)
                                           gCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATAACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCg                                                                                                           <  1:3034295/149‑1 (MQ=255)
                                             cccAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGt                                                                                                         <  2:1741484/149‑1 (MQ=255)
                                             cccAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGt                                                                                                         >  2:2332051/1‑149 (MQ=255)
                                              ccAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTg                                                                                                        <  2:347981/149‑1 (MQ=255)
                                              ccAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTg                                                                                                        <  2:521789/149‑1 (MQ=255)
                                               cAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGa                                                                                                       >  2:487103/1‑149 (MQ=255)
                                                 gCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGata                                                                                                     >  1:383670/1‑149 (MQ=255)
                                                      cacGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATAACCACCGCTTTGAGGAGGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCaa                                                                                                >  1:2438417/1‑149 (MQ=255)
                                                          gTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCa                                                                                            <  1:1959372/149‑1 (MQ=255)
                                                              ggTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCAttgt                                                                                        >  2:1009808/1‑149 (MQ=255)
                                                               gTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCAttgtt                                                                                       <  2:1566825/149‑1 (MQ=255)
                                                                 ccGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATtgttgt                                                                                     <  2:1153652/149‑1 (MQ=255)
                                                                 ccGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATAACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATtgttgt                                                                                     <  2:3909116/149‑1 (MQ=255)
                                                                  cGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTtgtg                                                                                    <  1:2059476/149‑1 (MQ=255)
                                                                    tAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATAACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGcc                                                                                  >  2:3822954/1‑149 (MQ=255)
                                                                      aCGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGcctc                                                                                >  2:895263/1‑149 (MQ=255)
                                                                      aCGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGcctc                                                                                >  1:15543/1‑149 (MQ=255)
                                                                       cGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGcctcc                                                                               >  2:1693297/1‑149 (MQ=255)
                                                                        ggTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGcctcct                                                                              <  1:2280409/149‑1 (MQ=255)
                                                                        ggTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGcctcct                                                                              <  1:3482270/149‑1 (MQ=255)
                                                                        ggTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGcctcct                                                                              <  1:3482180/149‑1 (MQ=255)
                                                                         gTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCtt                                                                             >  1:2795635/1‑149 (MQ=255)
                                                                         gTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATAACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCtt                                                                             <  1:50175/149‑1 (MQ=255)
                                                                          ttCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTg                                                                            <  2:1826985/149‑1 (MQ=255)
                                                                             tGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTgaga                                                                         >  2:2658032/1‑149 (MQ=255)
                                                                              ggAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGAt                                                                        <  1:1807775/149‑1 (MQ=255)
                                                                              ggAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGAt                                                                        >  1:2863182/1‑149 (MQ=255)
                                                                               gAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATc                                                                       <  1:2208556/149‑1 (MQ=255)
                                                                                 aCAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCt                                                                     <  1:3251960/149‑1 (MQ=255)
                                                                                   aGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCa                                                                   >  2:288855/1‑149 (MQ=255)
                                                                                      cccAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGa                                                                <  2:652236/149‑1 (MQ=255)
                                                                                      cccAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGa                                                                <  2:828487/149‑1 (MQ=255)
                                                                                      cccAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATAACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGa                                                                <  2:3640569/149‑1 (MQ=255)
                                                                                       ccAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGAt                                                               <  2:3198692/149‑1 (MQ=255)
                                                                                            agGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATgcgcg                                                          >  1:1361663/1‑149 (MQ=255)
                                                                                              gCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCgcc                                                        >  2:2544643/1‑149 (MQ=255)
                                                                                                       gCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCAc                                               <  2:4212610/149‑1 (MQ=255)
                                                                                                         cAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCg                                             <  1:288855/149‑1 (MQ=255)
                                                                                                           aaGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGa                                           <  2:3982453/149‑1 (MQ=255)
                                                                                                           aaGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATAACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGa                                           >  2:2804396/1‑149 (MQ=255)
                                                                                                            aGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAg                                          >  1:3214575/1‑149 (MQ=255)
                                                                                                             gCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGc                                         >  1:4130536/1‑149 (MQ=255)
                                                                                                               tCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCaa                                       >  2:1449427/1‑149 (MQ=255)
                                                                                                                 aTGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATg                                     >  1:1761867/1‑149 (MQ=255)
                                                                                                                  tGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCATCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGc                                    <  2:2614797/149‑1 (MQ=255)
                                                                                                                  tGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGc                                    <  1:1514768/149‑1 (MQ=255)
                                                                                                                   gAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGcc                                   >  2:809935/1‑149 (MQ=255)
                                                                                                                             cAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATAccc                         <  2:3955686/149‑1 (MQ=255)
                                                                                                                               ggTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCAt                       <  1:3262714/149‑1 (MQ=255)
                                                                                                                               ggTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCAt                       >  1:2667994/1‑149 (MQ=255)
                                                                                                                                gTGACAACCACCAGCAGGATAACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATc                      <  1:882593/149‑1 (MQ=255)
                                                                                                                                 tGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCg                     >  1:4077287/1‑149 (MQ=255)
                                                                                                                                  gACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCgg                    >  2:1862477/1‑149 (MQ=255)
                                                                                                                                   aCAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGc                   <  2:56216/149‑1 (MQ=255)
                                                                                                                                   aCAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGc                   >  1:1677629/1‑149 (MQ=255)
                                                                                                                                     aaCCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCaa                 <  1:4395370/149‑1 (MQ=255)
                                                                                                                                     aaCCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCaa                 >  2:2232447/1‑149 (MQ=255)
                                                                                                                                     aaCCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCaa                 <  2:4574516/149‑1 (MQ=255)
                                                                                                                                            agcagGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCAcc          >  2:3533725/1‑149 (MQ=255)
                                                                                                                                             gcagGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCAcct         <  1:1749404/149‑1 (MQ=255)
                                                                                                                                             gcagGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCAcct         >  2:398494/1‑149 (MQ=255)
                                                                                                                                                ggATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCAcctgcc      >  2:3814023/1‑149 (MQ=255)
                                                                                                                                                ggATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCAcctgcc      <  2:1008627/149‑1 (MQ=255)
                                                                                                                                                ggATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCAcctgcc      <  2:3409083/149‑1 (MQ=255)
                                                                                                                                                ggATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCAcctgcc      <  2:2417796/149‑1 (MQ=255)
                                                                                                                                                  aTCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCAcctgccct    >  2:660475/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TAAAGAACATTTTGATCATGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGC  >  CP009273/2390714‑2391010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑