Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,609,187 C→A 51.4% A18A (GCG→GCT)  yfgO ← putative UPF0118 family inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,609,1870CA51.4% 89.0 / inf 359A18A (GCG→GCT) yfgOputative UPF0118 family inner membrane permease
Reads supporting (aligned to +/- strand):  ref base C (63/111);  new base A (90/95);  total (153/206)
Fisher's exact test for biased strand distribution p-value = 1.90e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.08e-01

Only 100 of 361 total aligned reads displayed.

AGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTATGACGGATTCAGG  >  CP009273/2609039‑2609333
                                                                                                                                                    |                                                                                                                                                  
  cggcggGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGc                                                                                                                                                  <  1:1700320/149‑1 (MQ=255)
  cggcggGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGc                                                                                                                                                  <  1:1756895/149‑1 (MQ=255)
       ggAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCACGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCg                                                                                                                                             >  1:2470094/1‑149 (MQ=255)
       ggAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCg                                                                                                                                             >  2:3837275/1‑149 (MQ=255)
       ggAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATCCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCg                                                                                                                                             >  2:2979202/1‑149 (MQ=255)
        gAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCgg                                                                                                                                            <  2:2779243/149‑1 (MQ=255)
           cagcCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTc                                                                                                                                         <  2:2306414/149‑1 (MQ=255)
             gcCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGc                                                                                                                                       >  1:593736/1‑149 (MQ=255)
             gcCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGc                                                                                                                                       >  1:889144/1‑149 (MQ=255)
              ccAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCt                                                                                                                                      >  1:4580285/1‑149 (MQ=255)
                aaTAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTaa                                                                                                                                    >  2:929976/1‑149 (MQ=255)
                aaTAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTaa                                                                                                                                    >  2:3552626/1‑149 (MQ=255)
                  tAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTaaaa                                                                                                                                  <  2:744038/149‑1 (MQ=255)
                   aGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAAc                                                                                                                                 <  2:682418/149‑1 (MQ=255)
                    gATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAAcg                                                                                                                                <  2:3760685/149‑1 (MQ=255)
                    gATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAAcg                                                                                                                                >  2:539155/1‑149 (MQ=255)
                     aTTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAAcgg                                                                                                                               <  1:1170500/149‑1 (MQ=255)
                      ttGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATACTGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAAcggc                                                                                                                              <  2:3042259/149‑1 (MQ=255)
                      ttGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAAcggc                                                                                                                              <  2:3992753/149‑1 (MQ=255)
                      ttGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAAcggc                                                                                                                              >  1:1992339/1‑149 (MQ=255)
                          aGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGgcggcg                                                                                                                          >  1:2857740/1‑149 (MQ=255)
                              gCAGGGTTGGCCATTCCAGCAAATAGCCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCAATAAAGAAAAATATAATGCCAAATCCGGCAAATAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATAc                                                                                                                      <  1:3956925/149‑1 (MQ=255)
                               cACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATAcc                                                                                                                     <  1:1148136/149‑1 (MQ=255)
                                aCGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCa                                                                                                                    <  1:2730740/149‑1 (MQ=255)
                                  ggTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCAtt                                                                                                                  <  2:180317/149‑1 (MQ=255)
                                    ttGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGc                                                                                                                >  2:2814852/1‑149 (MQ=255)
                                    ttGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGc                                                                                                                >  2:1828472/1‑149 (MQ=255)
                                       gCCATTCCAGCAAATAGGCCAGCACAATCGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATc                                                                                                             <  2:1968094/149‑1 (MQ=255)
                                        ccATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCa                                                                                                            >  2:1311345/1‑149 (MQ=255)
                                          aTTCCAGCAAATAGGCCACCACAATACCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAAc                                                                                                          <  1:4295390/149‑1 (MQ=255)
                                              cAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACAttt                                                                                                      >  1:2441084/1‑149 (MQ=255)
                                                  aaaTAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAg                                                                                                  <  1:662316/149‑1 (MQ=255)
                                                   aaTAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGc                                                                                                 <  1:1888766/149‑1 (MQ=255)
                                                    aTAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCa                                                                                                >  1:3750770/1‑149 (MQ=255)
                                                    aTAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCa                                                                                                >  1:4219232/1‑149 (MQ=255)
                                                    aTAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGACACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCa                                                                                                <  2:3151956/149‑1 (MQ=255)
                                                     tAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCAt                                                                                               >  1:453753/1‑149 (MQ=255)
                                                     tAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCAt                                                                                               <  1:331435/149‑1 (MQ=255)
                                                      aGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATa                                                                                              >  2:1515350/1‑149 (MQ=255)
                                                      aGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATa                                                                                              >  2:1006325/1‑149 (MQ=255)
                                                      aGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATa                                                                                              <  2:446367/149‑1 (MQ=255)
                                                          cAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGa                                                                                          <  1:3499574/149‑1 (MQ=255)
                                                           aGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGAc                                                                                         <  1:4111733/149‑1 (MQ=255)
                                                            gCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGAcc                                                                                        >  1:3024982/1‑149 (MQ=255)
                                                                 aTAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTccc                                                                                   >  1:2592057/1‑149 (MQ=255)
                                                                  tAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCt                                                                                  <  2:4661788/149‑1 (MQ=255)
                                                                  tAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCt                                                                                  <  2:3452398/149‑1 (MQ=255)
                                                                   aGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTg                                                                                 >  1:2280534/1‑149 (MQ=255)
                                                                     ccaccaGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGaa                                                                               <  2:2522326/149‑1 (MQ=255)
                                                                     ccaccaGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGAAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGaa                                                                               <  1:9344/149‑1 (MQ=255)
                                                                      caccaGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAAc                                                                              <  1:53520/149‑1 (MQ=255)
                                                                       accaGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAccc                                                                             <  2:235735/149‑1 (MQ=255)
                                                                       accaGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAAcc                                                                             <  2:4398940/149‑1 (MQ=255)
                                                                           gCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCaa                                                                         >  2:152837/1‑149 (MQ=255)
                                                                              aCGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAagag                                                                      <  2:804967/149‑1 (MQ=255)
                                                                                  agcaagcaGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCggg                                                                  <  2:422645/149‑1 (MQ=255)
                                                                                    caagcaGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGAt                                                                <  2:453788/149‑1 (MQ=255)
                                                                                    caagcaGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGAt                                                                <  1:526237/149‑1 (MQ=255)
                                                                                      agcaGGCCACTAAAGAAAAATATAATGCCAAATCCGGAAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTg                                                              <  1:1107316/149‑1 (MQ=255)
                                                                                      agcaGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTg                                                              >  1:2575856/1‑149 (MQ=255)
                                                                                         aGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCACGAGCGGGATTGCGa                                                           >  2:4174720/1‑149 (MQ=255)
                                                                                                 aaaGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAAt                                                   <  1:76615/149‑1 (MQ=255)
                                                                                                 aaaGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAAt                                                   >  2:1272688/1‑149 (MQ=255)
                                                                                                  aaGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAAtt                                                  <  1:1308934/149‑1 (MQ=255)
                                                                                                   aGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTg                                                 >  1:291535/1‑149 (MQ=255)
                                                                                                    gAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGt                                                <  2:1100846/149‑1 (MQ=255)
                                                                                                     aaaaaGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTa                                               >  1:4670694/1‑149 (MQ=255)
                                                                                                      aaaaGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTAt                                              <  1:3199975/149‑1 (MQ=255)
                                                                                                          gATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAAAATTTCGAGCATAAAGACCTTACCTGAACCTCAAGAGCGGGATTGCGATACGCAATTGTATCGaa                                          >  1:4365540/1‑149 (MQ=255)
                                                                                                           aTAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGaaa                                         <  2:62433/149‑1 (MQ=255)
                                                                                                           aTAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGaaa                                         <  2:1367751/149‑1 (MQ=255)
                                                                                                              aTGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGt                                      <  2:1632029/149‑1 (MQ=255)
                                                                                                              aTGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGt                                      >  1:3139473/1‑149 (MQ=255)
                                                                                                               tGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTc                                     <  2:2454884/149‑1 (MQ=255)
                                                                                                                gCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTca                                    <  2:3642452/149‑1 (MQ=255)
                                                                                                                gCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTca                                    >  2:3719186/1‑149 (MQ=255)
                                                                                                                 ccAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTcac                                   <  1:811916/149‑1 (MQ=255)
                                                                                                                    aaTCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACaaa                                >  2:325896/1‑149 (MQ=255)
                                                                                                                    aaTCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACaaa                                >  2:406576/1‑149 (MQ=255)
                                                                                                                     aTCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACaaaa                               >  2:3793684/1‑149 (MQ=255)
                                                                                                                      tCCGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACaaaaa                              >  1:1487772/1‑149 (MQ=255)
                                                                                                                       ccGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACaaaaaa                             <  1:1076626/149‑1 (MQ=255)
                                                                                                                       ccGGCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACaaaaaa                             <  1:94200/149‑1 (MQ=255)
                                                                                                                         ggCAACTAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGa                           <  1:4033171/149‑1 (MQ=255)
                                                                                                                            aaCTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACtt                        >  2:3300607/1‑149 (MQ=255)
                                                                                                                            aaCTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACtt                        <  1:3858690/149‑1 (MQ=255)
                                                                                                                               tAAAATAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAACAGACTTCGc                     <  1:2646358/149‑1 (MQ=255)
                                                                                                                                aaaaTAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCCTCAACAATTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCt                    >  1:1592445/1‑149 (MQ=255)
                                                                                                                                 aaaTAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCtt                   <  2:3809648/149‑1 (MQ=255)
                                                                                                                                 aaaTAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCtt                   <  2:1210687/149‑1 (MQ=255)
                                                                                                                                   aTAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCtttt                 <  2:2171497/149‑1 (MQ=255)
                                                                                                                                    tAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCttttt                >  1:3550910/1‑149 (MQ=255)
                                                                                                                                    tAACCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCttttt                >  2:199199/1‑149 (MQ=255)
                                                                                                                                      aCCAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTAt              >  1:4390384/1‑149 (MQ=255)
                                                                                                                                       ccAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTATg             >  1:3443974/1‑149 (MQ=255)
                                                                                                                                        cAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTATGa            >  2:2046388/1‑149 (MQ=255)
                                                                                                                                        cAGCAAGGCAATAGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTATGa            <  2:889144/149‑1 (MQ=255)
                                                                                                                                            aaGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTATGACGGa        <  1:2231181/149‑1 (MQ=255)
                                                                                                                                              ggCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAATACTTCGCTTTTTATGACGGAtt      >  1:2065670/1‑149 (MQ=255)
                                                                                                                                               gCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTATGACGGATTc     <  2:3080314/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAATCGCTTCCGGGTCGCTAAAACGGCGGCGATACCATTGCATCAACATTTCGAGCATAAAGACCTTCCCTGAACCTCAAGAGCGGGATTGCGATCCGCAATTGTATCGAAATGTCACAAAAAAGACTTCGCTTTTTATGACGGATTCAGG  >  CP009273/2609039‑2609333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑