Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,832,598 T→G 11.7% K9T (AAG→ACG)  ascG ← asc operon transcriptional repressor; prpBC operon repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,832,5980TG11.7% 582.4 / 10.2 234K9T (AAG→ACG) ascGasc operon transcriptional repressor; prpBC operon repressor
Reads supporting (aligned to +/- strand):  ref base T (63/135);  new base G (29/4);  total (95/139)
Fisher's exact test for biased strand distribution p-value = 1.08e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

ACTCTTCGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCA  >  CP009273/2832450‑2832746
                                                                                                                                                    |                                                                                                                                                    
aCTCTTCGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCt                                                                                                                                                      >  2:2799936/1‑149 (MQ=255)
  tctTCGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTc                                                                                                                                                    >  2:4644494/1‑149 (MQ=255)
  tctTCGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCCGCGCGCGTc                                                                                                                                                    >  1:888589/1‑149 (MQ=255)
      cGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCa                                                                                                                                                <  2:2467797/149‑1 (MQ=255)
           aCAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCGTCGCCACATcc                                                                                                                                           >  1:4322896/1‑149 (MQ=255)
            cAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCCGCGCGCGTCGCCACATCCa                                                                                                                                          >  1:4150441/1‑149 (MQ=255)
             aGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAg                                                                                                                                         >  1:718480/1‑149 (MQ=255)
             aGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAg                                                                                                                                         <  2:1234754/149‑1 (MQ=255)
              gATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGc                                                                                                                                        >  2:3594997/1‑149 (MQ=255)
               aTTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTGTTGAAACCCCCGCGCGCGTCGCCACATCCaccc                                                                                                                                       >  1:2430286/1‑146 (MQ=255)
                ttGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCAt                                                                                                                                      <  2:2934108/149‑1 (MQ=255)
                ttGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCAt                                                                                                                                      >  2:113989/1‑149 (MQ=255)
                ttGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCGTCGCCACTTCCAGCAt                                                                                                                                      >  1:1105486/1‑149 (MQ=255)
                 tGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCAtc                                                                                                                                     <  2:1528953/149‑1 (MQ=255)
                   cgcgcCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCAtcgt                                                                                                                                   <  2:2136658/149‑1 (MQ=255)
                   cgcgcCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCAtcgt                                                                                                                                   <  1:4490017/149‑1 (MQ=255)
                   cgcgcCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCAtcgt                                                                                                                                   <  1:2986248/149‑1 (MQ=255)
                      gcCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGTAACCCCGGCGCGCGTCGCCCCATCCAGCCTcgtcgt                                                                                                                                >  1:1187172/1‑149 (MQ=255)
                      gcCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATcgtcgt                                                                                                                                <  1:74705/149‑1 (MQ=255)
                        cAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGtca                                                                                                                              <  2:1819332/149‑1 (MQ=255)
                         aGCAAGTGTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTGTAGTCTCCTGGCTGACGTAGCCAGTACCTGAAAGCACGCGGGACACGGTCGCTTTTGAAACCCCGGCGCGCTGCGCCCCTTCCAGCAGCGGCGtcat                                                                                                                             >  2:542347/1‑149 (MQ=255)
                           cAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGtcatca                                                                                                                           <  2:1769291/149‑1 (MQ=255)
                            aaGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTcatcat                                                                                                                          <  2:1790636/149‑1 (MQ=255)
                              gTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCAttt                                                                                                                        <  1:944999/149‑1 (MQ=255)
                               tttGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCAtttt                                                                                                                       <  1:325843/149‑1 (MQ=255)
                                ttGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTc                                                                                                                      <  1:2625627/149‑1 (MQ=255)
                                ttGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTc                                                                                                                      <  2:4157480/149‑1 (MQ=255)
                                 tGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCa                                                                                                                     <  2:2197080/149‑1 (MQ=255)
                                  ggACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTGTTGAAACCCCCGCGCGCTTCGCCACATCCACCAACGTCGTCATCATTATCaa                                                                                                                    >  1:4674424/1‑148 (MQ=255)
                                      ggTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCt                                                                                                                <  1:2919941/149‑1 (MQ=255)
                                       gTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCtt                                                                                                               <  2:871329/149‑1 (MQ=255)
                                         aaCCGCTCTCTTCTACCGCCTGAAACACGCGATCTTCAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTa                                                                                                             <  2:2048348/149‑1 (MQ=255)
                                          aCCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTac                                                                                                            <  2:4365074/149‑1 (MQ=255)
                                            cGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGTAACCCCGGCGCGCTTCGCCACATCCAGCATCGTCGTCATCAATTTAATACCATTaaac                                                                                                          >  1:3765274/1‑149 (MQ=255)
                                            cGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCGTTTTAAACCCCGGCGCGCGTCGCCACATCCAGCAACGTCGTCATAATTTTCAACCCATTaaaa                                                                                                          >  1:1940389/1‑146 (MQ=255)
                                              ctctctTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGc                                                                                                        <  2:3939382/149‑1 (MQ=255)
                                                 tcttctACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAAt                                                                                                     >  2:3120256/1‑149 (MQ=255)
                                                  cttctACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGTAACCCCCGCGCGCGTCGCCACTTCCAGCAACGTCGTCATCAATTTAATACCCTTAAACGccaac                                                                                                    >  1:4030650/1‑145 (MQ=255)
                                                  cttctACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATc                                                                                                    <  1:2169637/149‑1 (MQ=255)
                                                  cttctACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATc                                                                                                    <  1:3025142/149‑1 (MQ=255)
                                                   ttctACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCGTTACACGCCATCa                                                                                                   >  2:441621/1‑149 (MQ=255)
                                                    tctACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCaa                                                                                                  <  2:2172689/149‑1 (MQ=255)
                                                    tctACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCaa                                                                                                  <  2:2157759/149‑1 (MQ=255)
                                                      tACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGTAACCCCGGCGCGCGTCGCCACATCCACCCTCGTCGTCCAAAATTTAATCCCATTACACACAATAAAcc                                                                                                >  1:3293893/1‑148 (MQ=255)
                                                          gCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAgt                                                                                            <  1:4466834/149‑1 (MQ=255)
                                                            cTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCATCGCCACATCCAGCATCGTCGTCAACATTTTCATCCCTTTACACGCAAACAAAGCAgtgt                                                                                          >  2:4572697/1‑149 (MQ=255)
                                                               aaaCACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACt                                                                                       <  1:4388617/149‑1 (MQ=255)
                                                                 acacGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGc                                                                                     <  2:1900104/149‑1 (MQ=255)
                                                                 acacGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCGTCGCCACTTCCAGCATCGTCGTCAACATTTTCATACCTTTACACGCAAAAAACCCAGTGTAcatc                                                                                     >  2:1611193/1‑146 (MQ=255)
                                                                   acGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCGTCGCCCCATCCACCAACGTCGTCATAATTTTCATACCATTAAAACCAAAAAACACACGGTACTGCAc                                                                                   >  1:1282056/1‑149 (MQ=255)
                                                                     gcgATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCGTCGCCACTTCCAGCCACGTCGTCATCAATTTAAACCCTTTAAACGCAAACAACGCAGTGGACTGCACCg                                                                                 >  1:680452/1‑149 (MQ=255)
                                                                     gcgATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCGTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCg                                                                                 >  2:2956555/1‑149 (MQ=255)
                                                                       gATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGtt                                                                               <  1:4676000/149‑1 (MQ=255)
                                                                       gATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGtt                                                                               <  2:2081477/149‑1 (MQ=255)
                                                                       gATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGtt                                                                               <  1:15001/149‑1 (MQ=255)
                                                                         tCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTTAAAACCCCGCGCGCGTGGCCACAACCAGCATAGTCGTCAAAAATTTAATACCATTAAAAGCAAAAAACCCACTGTACAGGAcccgttt                                                                             >  1:3674079/1‑144 (MQ=255)
                                                                               gTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGAtt                                                                       <  2:4260042/149‑1 (MQ=255)
                                                                                tctcCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTGTTGTAACCCCCGCGCGCGTCGCCACATCCAGCCACGTCGTCATCAATTTAAAACCGATACACGCAAACAACACAGTGTAAGGCACCCTTTGCCGAATg                                                                      >  1:3332586/1‑149 (MQ=255)
                                                                                    cTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCt                                                                  <  1:971603/149‑1 (MQ=255)
                                                                                     tGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCtt                                                                 <  2:3855294/149‑1 (MQ=255)
                                                                                     tGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCtt                                                                 <  2:158231/149‑1 (MQ=255)
                                                                                      ggCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCCGCGCGCTTCGCCACATCCAGCAACGTCGTCATCATTTTCCTCCCGTTACACGCAATCAACGCAGTGTACGGCACCGGTTGCCGAATGTccgtt                                                                >  1:4200408/1‑146 (MQ=255)
                                                                                       gCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGc                                                               <  2:3958402/149‑1 (MQ=255)
                                                                                         tGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCCCGCTTCGCCACATACAGCAACGTCGTCATCAAATTCATCACAATACACGCAAACAAAGCAGTGTACAGCACCCTTTGCCCATTGTCCATGcac                                                             >  2:3215780/1‑149 (MQ=255)
                                                                                             gTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATc                                                         <  1:159244/149‑1 (MQ=255)
                                                                                              tAGCCCTTACCTGAAAGCACGCGGGACACGGTCGCTTTTGAAACCCCGGCGCGCGTCGCCCCGTCCCGCCGCGTCGGCATCATTTTCCTCCCGTTACCCGCACTCCACCCCGGGTACGGCCCCGGTTGCCGATTGTCCGTGCCCCATcg                                                        >  2:1028971/1‑149 (MQ=255)
                                                                                              tAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcg                                                        <  2:1861580/149‑1 (MQ=255)
                                                                                                gCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggc                                                      <  1:3858362/149‑1 (MQ=255)
                                                                                                  cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTAGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAAAAAACGCAGTGTACTGCACCGTTTTCCGATTGTACTTGCACAATcggcgg                                                    >  2:27056/1‑149 (MQ=255)
                                                                                                           aaaGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAatat                                           <  1:2657473/149‑1 (MQ=255)
                                                                                                            aaGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATAtt                                          <  1:2538417/149‑1 (MQ=255)
                                                                                                            aaGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATAtt                                          <  1:2538543/149‑1 (MQ=255)
                                                                                                              gCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCa                                        <  1:3421330/149‑1 (MQ=255)
                                                                                                              gCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCa                                        <  1:2799936/149‑1 (MQ=255)
                                                                                                                aCGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCCTCGTCGTCATAATTTTCATCCCTTTACACGCAATAAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAgg                                      >  2:4135648/1‑149 (MQ=255)
                                                                                                                aCGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATACCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTTCACAATCGGCGGGAAAAATATTCAgg                                      >  2:3003471/1‑149 (MQ=255)
                                                                                                                  gcgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTg                                    <  2:4545336/149‑1 (MQ=255)
                                                                                                                   cgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCCACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGa                                   <  2:1349430/149‑1 (MQ=255)
                                                                                                                   cgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACGTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGa                                   <  2:2127220/149‑1 (MQ=255)
                                                                                                                   cgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCGTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGa                                   <  2:3686560/149‑1 (MQ=255)
                                                                                                                    gggAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAc                                  <  2:3443786/149‑1 (MQ=255)
                                                                                                                     ggAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAcc                                 <  1:1058860/149‑1 (MQ=255)
                                                                                                                     ggAAACGGTCGCTTTTGAAACCCCCGCCCGCTTCGCCACATCCACCATCGTCGTCATCCATTTAATCCCATTACACCCAATCAACCCAGTGTACTGCACCGTTTGCCGAATGTCCTTGCCCAAACGGCGGGAAAAATATTCAGGTGAcc                                 >  2:2519864/1‑149 (MQ=255)
                                                                                                                          cGGTCGCTTTTGAAACCCCGGCGCGCTTCTCCACTTCCAGCATCGTCGTCATCATTTTCATCGCTTTACACGCTATCTACGGAGTGTTCTGCACCGTTTGCCGATTGTCCTTGCACATTCGGCGGGTTAAATATTCAGGTGACCGGttt                            <  2:3811744/149‑1 (MQ=255)
                                                                                                                           ggTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTc                           <  2:4072935/149‑1 (MQ=255)
                                                                                                                              cGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcaca                        >  1:3383841/1‑149 (MQ=255)
                                                                                                                              cGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATAATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAAAATTAAGGTGACCGGTTTcaca                        >  1:3597663/1‑149 (MQ=255)
                                                                                                                                cTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaaa                      >  2:4026360/1‑149 (MQ=255)
                                                                                                                                   ttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAatat                   <  1:1405962/149‑1 (MQ=255)
                                                                                                                                     gAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaa                 <  2:3061084/149‑1 (MQ=255)
                                                                                                                                      aaaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaa                <  1:1044998/149‑1 (MQ=255)
                                                                                                                                      aaaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaa                >  1:1859518/1‑149 (MQ=255)
                                                                                                                                      aaaCCCCGGCGCGCGTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaa                <  1:313804/149‑1 (MQ=255)
                                                                                                                                         ccccGGCGAGCTTCGCCACTACCAGCCTCGACGTAAAAATTTTCAAACCTTTACACCCAATCAACGCAGTGTACTGCACCGTTTGCCGAATGTCAAAAAACAAAACACGGGAAAAAAATAAAGGTGACCGGTTTAACAAATaaaaaaaa             >  2:1280004/1‑149 (MQ=255)
                                                                                                                                           ccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATg           <  1:3764795/149‑1 (MQ=255)
                                                                                                                                             ggCGCGCTTCGCCACTTCAAGCATCGTCGTCATCATTTTCATCCCTTTCCACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGaa         <  2:2556175/149‑1 (MQ=255)
                                                                                                                                                gcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACaa      <  1:2723141/149‑1 (MQ=255)
                                                                                                                                                gcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACaa      >  2:904291/1‑149 (MQ=255)
                                                                                                                                                   cTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTc   >  2:2288088/1‑149 (MQ=255)
                                                                                                                                                    ttCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCa  >  2:375080/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACTCTTCGCCGACAGATTGCGCGCCAGCAAGTTTGGACGGTAACCGCTCTCTTCTACCGCCTGAAACACGCGATCTTTAGTCTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCA  >  CP009273/2832450‑2832746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑