BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,024,983	C→G	68.9%	G86G (GGC→GGG)	uacT →	uric acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,024,983	0	C	G	68.9%	560.4 / inf	296	G86G (GGC→GGG)	uacT	uric acid permease
Reads supporting (aligned to +/- strand): ref base C (43/49); new base G (102/102); total (145/151)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 299 total aligned reads displayed.

ACGTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACATTATTA  >  CP009273/3024835‑3025130

acGTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGc                                                                                                                                                     <  1:2104071/149‑1 (MQ=255)

  gTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTATCAAAAGAAGCTATTGCGATTCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTTCAATGTATAGGTATAGGGCg                                                                                                                                                   >  2:4376397/1‑149 (MQ=255)

   tCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATTATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTTCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATAGGgcgc                                                                                                                                                  >  2:205163/1‑149 (MQ=255)

    ccTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCt                                                                                                                                                 <  1:4101229/149‑1 (MQ=255)

    ccTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCt                                                                                                                                                 <  2:1472287/149‑1 (MQ=255)

     cTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCtt                                                                                                                                                <  1:1556779/149‑1 (MQ=255)

       tGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTa                                                                                                                                              <  1:4524305/149‑1 (MQ=255)

         tCATGTACGCAGGTGCAGTCGCTGGTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATg                                                                                                                                            <  1:1362646/149‑1 (MQ=255)

           aTGTACGCAGGTGCAGTCGCTGTTACTCTTATGATTGGTGACCGACGGGGCCAATCAAAATAAGCTATTGCGATTCTCATTAGCAAGAAACTCTTTTGCTTCGGGATCGTCACAATATTTCAATGTATCGGTATCGGGCGCGTTATtgg                                                                                                                                          >  2:1214229/1‑149 (MQ=255)

            tGTACGCAGGTGCAGTCGCGGTTCCGCTTATGATTGGTGACCGCCTGGGCCACTCAAAAGAAGCTATTGCGATGCGCATTAGCGCGGATCTCTTTTGCTGCGGGATCGTCACATTATTTCAAAGTATCGGGATCGGGCGCGTTATgggg                                                                                                                                         >  1:776300/1‑149 (MQ=255)

             gTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTATCTCGGATCTCTTTTTCTTCGGGATCGTCACATTATTGCAATTTATAGGTATCGGGCGCTTTATTGGGa                                                                                                                                        >  2:4013699/1‑149 (MQ=255)

             gTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGa                                                                                                                                        <  2:4637974/149‑1 (MQ=255)

              tACGCAGGTGCAGTCGCTGTTCCACTTATGATTGGTGAACGACTGGGCCACTCAAAAGAAGCTATTGCGATGCTCATTATCTAGGATGTCTTTTTCTTCGGGATCGTCACATTATTTGAATGTATCGGTATCGGGCGCTTTATggggtt                                                                                                                                       >  2:2373292/1‑147 (MQ=255)

                   aGGTGCAGTCGCTGTTCCTCTTATGATTGGTTACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCACATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGcc                                                                                                                                  >  2:1143717/1‑149 (MQ=255)

                   aGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGcc                                                                                                                                  <  2:1369743/149‑1 (MQ=255)

                   aGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGcc                                                                                                                                  <  2:3196567/149‑1 (MQ=255)

                   aGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGcc                                                                                                                                  <  1:3490428/149‑1 (MQ=255)

                   aGGTGCAGTCGCTGGTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGcc                                                                                                                                  <  2:3704999/149‑1 (MQ=255)

                    ggTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCt                                                                                                                                 <  2:713713/149‑1 (MQ=255)

                        cAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCg                                                                                                                             <  1:2135019/149‑1 (MQ=255)

                         aGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCgg                                                                                                                            <  2:575710/149‑1 (MQ=255)

                           tCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTg                                                                                                                          >  1:3077240/1‑149 (MQ=255)

                           tCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTg                                                                                                                          >  1:1296634/1‑149 (MQ=255)

                              cTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGAtt                                                                                                                       >  2:3771167/1‑149 (MQ=255)

                                gTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTAt                                                                                                                     >  2:2839771/1‑149 (MQ=255)

                                gTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTAt                                                                                                                     >  2:2426207/1‑149 (MQ=255)

                                  tCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGt                                                                                                                   <  2:3445941/149‑1 (MQ=255)

                                  tCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGt                                                                                                                   >  1:818625/1‑149 (MQ=255)

                                   ccTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTc                                                                                                                  <  2:322635/149‑1 (MQ=255)

                                       ttATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTg                                                                                                              >  1:3751265/1‑149 (MQ=255)

                                        gaTGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGa                                                                                                             <  2:19198/148‑1 (MQ=255)

                                         aTGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGAc                                                                                                            <  1:3886537/149‑1 (MQ=255)

                                          tGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCGTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGAcc                                                                                                           >  1:638218/1‑149 (MQ=255)

                                           gATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCt                                                                                                          <  1:1828371/149‑1 (MQ=255)

                                            aTTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCtt                                                                                                         <  2:511682/149‑1 (MQ=255)

                                            aTTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCtt                                                                                                         <  1:1182938/149‑1 (MQ=255)

                                             ttGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCGTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCttt                                                                                                        >  2:1948243/1‑149 (MQ=255)

                                               ggTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCGTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTtgc                                                                                                      >  1:1036376/1‑149 (MQ=255)

                                               ggTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTtgc                                                                                                      >  1:2037953/1‑149 (MQ=255)

                                                gTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTtgct                                                                                                     <  2:419087/149‑1 (MQ=255)

                                                   aCCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTgctgct                                                                                                  >  1:4266579/1‑149 (MQ=255)

                                                   aCCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTgctgct                                                                                                  >  1:4028034/1‑149 (MQ=255)

                                                    ccGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGctgctg                                                                                                 <  2:2756599/149‑1 (MQ=255)

                                                       aCTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTaa                                                                                              >  1:4160110/1‑149 (MQ=255)

                                                       aCTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTaa                                                                                              >  2:2503332/1‑149 (MQ=255)

                                                          gggCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAacac                                                                                           >  2:2566467/1‑149 (MQ=255)

                                                           ggCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACAcc                                                                                          >  2:665162/1‑149 (MQ=255)

                                                               tctcAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATg                                                                                      >  1:3308442/1‑149 (MQ=255)

                                                                  cAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATa                                                                                   >  2:1602078/1‑149 (MQ=255)

                                                                    aaaGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGc                                                                                 >  1:4618517/1‑149 (MQ=255)

                                                                     aagaagCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGcc                                                                                >  1:457805/1‑149 (MQ=255)

                                                                     aagaagCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTAGTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGcc                                                                                <  1:3731057/149‑1 (MQ=255)

                                                                         agCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTg                                                                            <  2:3425386/149‑1 (MQ=255)

                                                                          gCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTgg                                                                           >  1:2955869/1‑149 (MQ=255)

                                                                              ttGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATg                                                                       >  1:1022565/1‑149 (MQ=255)

                                                                                gCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGaa                                                                     >  2:4515141/1‑149 (MQ=255)

                                                                                   aTGCTGATTTGCCCGGATGTCTTTTGCTGCGTGATCGTAACATTAGTGCTATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAAccc                                                                  <  2:4587045/149‑1 (MQ=255)

                                                                                   aTGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAAccc                                                                  >  1:1102365/1‑149 (MQ=255)

                                                                                   aTGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAAccc                                                                  >  1:4350046/1‑149 (MQ=255)

                                                                                    tGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCg                                                                 >  1:2084543/1‑149 (MQ=255)

                                                                                       tCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGat                                                              <  1:2447881/149‑1 (MQ=255)

                                                                                        cATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGGATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGata                                                             <  2:3185279/149‑1 (MQ=255)

                                                                                         aTTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGatat                                                            >  2:3492613/1‑149 (MQ=255)

                                                                                          ttAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATc                                                           <  2:1507359/149‑1 (MQ=255)

                                                                                             gCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGc                                                        <  1:2905284/149‑1 (MQ=255)

                                                                                              cTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGcc                                                       <  1:3756180/149‑1 (MQ=255)

                                                                                               tCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCct                                                      <  1:3379264/149‑1 (MQ=255)

                                                                                                 ggATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctgc                                                    >  2:2706622/1‑149 (MQ=255)

                                                                                                  gATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctgct                                                   <  2:748050/149‑1 (MQ=255)

                                                                                                   aTCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctgctg                                                  <  2:1068690/149‑1 (MQ=255)

                                                                                                   aTCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCctgctg                                                  >  1:946180/1‑149 (MQ=255)

                                                                                                    tctcTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTTCTGCTGTAAAACCAAAGATAGCCAATGGGATGAAACCCGATATCGGCCAGCTgg                                                 >  1:1959884/1‑149 (MQ=255)

                                                                                                      tctTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTgggg                                               >  1:470425/1‑149 (MQ=255)

                                                                                                           tgctgcGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATAtt                                          >  2:1731393/1‑149 (MQ=255)

                                                                                                           tgctgcGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATAtt                                          <  2:3592095/149‑1 (MQ=255)

                                                                                                               gcGGGATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGt                                      <  1:2639763/149‑1 (MQ=255)

                                                                                                                  ggATCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGcc                                   <  1:2369062/149‑1 (MQ=255)

                                                                                                                     tCGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACt                                <  2:3057531/149‑1 (MQ=255)

                                                                                                                      cGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTa                               <  2:776306/149‑1 (MQ=255)

                                                                                                                      cGTCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTa                               <  1:69534/149‑1 (MQ=255)

                                                                                                                       gTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTAt                              <  2:4277313/149‑1 (MQ=255)

                                                                                                                        tCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATc                             <  1:368738/149‑1 (MQ=255)

                                                                                                                        tCACATTATTGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATc                             >  1:3548712/1‑149 (MQ=255)

                                                                                                                            attattGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATcgccg                         >  2:3561954/1‑149 (MQ=255)

                                                                                                                               attGCAAGGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCgg                      <  1:2426207/149‑1 (MQ=255)

                                                                                                                                ttGCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCggg                     <  2:3541381/149‑1 (MQ=255)

                                                                                                                                  gCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGtt                   >  2:1810682/1‑149 (MQ=255)

                                                                                                                                  gCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGtt                   <  1:3994013/149‑1 (MQ=255)

                                                                                                                                  gCAATGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCCGTGAAAATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGtt                   >  2:2640366/1‑149 (MQ=255)

                                                                                                                                    aaTGTATCGGTATCGGGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTAGGTGACCTTTGCTGATGTAACACCAATGATAGCAATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGtttt                 >  2:424764/1‑149 (MQ=255)

                                                                                                                                        tatcggtatcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATca             >  2:2285594/1‑149 (MQ=255)

                                                                                                                                        tatcggtatcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATca             >  2:2185439/1‑149 (MQ=255)

                                                                                                                                        tatcggtatcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATca             <  1:3211812/149‑1 (MQ=255)

                                                                                                                                          tcggtatcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATCTCGGTGACCTTTGCTGCTGTAACACCAATGATATCAATTGGGATGAACCCGGATATCGGCCTGCTGGGGATCTTTGGTTCCACTATCGCCGCGGGTTTTATcacc           >  2:3860940/1‑149 (MQ=255)

                                                                                                                                              tatcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACat       <  1:1842871/149‑1 (MQ=255)

                                                                                                                                              tatcggGCGCTTTATGGGGATCCGCATGCCGGTGATAATGTCGGTGACCTTTGCTTCAGAAAAACCAATGATAGCCAATGGGATGAACCCGGATAACGGCCTTCTGGGGATATTTGTTGACACTATCGCCGCGGGTTTTATCACCACat       >  2:2139117/1‑149 (MQ=255)

                                                                                                                                                tcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATTAAAGCCAATGGGATGAAACCGGATATCGGCCTGCTGGGGATAATTTGTGCCACTATCGCCGCGGGTTTTATCACCACatta     >  1:2195721/1‑149 (MQ=255)

                                                                                                                                                tcggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACatta     >  2:484379/1‑149 (MQ=255)

                                                                                                                                                 cggGCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACattat    <  2:4291883/149‑1 (MQ=255)

                                                                                                                                                   gCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACAttatta  <  1:2910964/149‑1 (MQ=255)

ACGTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGGTATCGGCCGCTTTATGGGGATCCGCCTGCCGGTGATTATGTCGGTGACCTTTGCTGCTGTAACACCAATGATAGCCATTGGGATGAACCCGGATATCGGCCTGCTGGGGATATTTGGTGCCACTATCGCCGCGGGTTTTATCACCACATTATTA  >  CP009273/3024835‑3025130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`