Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,033,430 G→T 13.6% D73Y (GAC→TAC)  fldB → flavodoxin 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,033,4300GT13.6% 883.9 / 91.5 264D73Y (GAC→TAC) fldBflavodoxin 2
Reads supporting (aligned to +/- strand):  ref base G (117/111);  new base T (18/18);  total (135/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 283 total aligned reads displayed.

TATTATCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGCTACTGGCCAACGGA  >  CP009273/3033282‑3033578
                                                                                                                                                    |                                                                                                                                                    
tattatCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTcg                                                                                                                                                      >  1:4381078/1‑149 (MQ=255)
 attatCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTcga                                                                                                                                                     <  1:3494438/149‑1 (MQ=255)
   tatCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTctacg                                                                                                                                                   >  1:905723/1‑149 (MQ=255)
   tatCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTcgacg                                                                                                                                                   >  2:318675/1‑149 (MQ=255)
    atCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTcgacga                                                                                                                                                  >  1:2564095/1‑149 (MQ=255)
     tCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCgacgac                                                                                                                                                 >  1:3006470/1‑149 (MQ=255)
     tCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCgacgac                                                                                                                                                 >  1:1516049/1‑149 (MQ=255)
      cGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGAcc                                                                                                                                                >  1:515671/1‑149 (MQ=255)
       ggCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGACATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCt                                                                                                                                               >  2:2495435/1‑149 (MQ=255)
       ggCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCt                                                                                                                                               <  1:2170151/149‑1 (MQ=255)
              aaCTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCtt                                                                                                                                        >  1:2375480/1‑149 (MQ=255)
               aCTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCTACGACCTGAACCTTg                                                                                                                                       <  1:3698865/149‑1 (MQ=255)
               aCTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTg                                                                                                                                       <  1:2165507/149‑1 (MQ=255)
                  ggTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAg                                                                                                                                    >  2:3718339/1‑149 (MQ=255)
                    tGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAAGGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGt                                                                                                                                  <  2:3121351/149‑1 (MQ=255)
                     gACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTa                                                                                                                                 >  1:534118/1‑149 (MQ=255)
                      aCCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTaa                                                                                                                                >  1:3604547/1‑149 (MQ=255)
                           aCATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAAttg                                                                                                                           <  1:4128003/149‑1 (MQ=255)
                           aCATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAAttg                                                                                                                           <  1:3849627/149‑1 (MQ=255)
                           aCATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAAttg                                                                                                                           >  2:1025914/1‑149 (MQ=255)
                            cATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAAttgt                                                                                                                          >  1:1002019/1‑149 (MQ=255)
                              tAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAAttgttg                                                                                                                        >  2:2716331/1‑149 (MQ=255)
                               aaCCTCACGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGGATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTgc                                                                                                                       <  2:2954627/149‑1 (MQ=255)
                                   tCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTg                                                                                                                   >  2:2202722/1‑149 (MQ=255)
                                        gacgacTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATgg                                                                                                              <  2:1817180/149‑1 (MQ=255)
                                         acgacTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATggg                                                                                                             >  1:3800148/1‑149 (MQ=255)
                                          cgacTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGc                                                                                                            >  2:3434280/1‑149 (MQ=255)
                                          cgacTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGc                                                                                                            <  1:4003847/149‑1 (MQ=255)
                                           gacTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCt                                                                                                           >  2:1472059/1‑149 (MQ=255)
                                           gacTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCt                                                                                                           >  1:1769117/1‑149 (MQ=255)
                                            acTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCtt                                                                                                          <  2:1156247/149‑1 (MQ=255)
                                            acTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCtt                                                                                                          <  1:3887326/149‑1 (MQ=255)
                                              tCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCTACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTgg                                                                                                        >  1:298209/1‑149 (MQ=255)
                                              tCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCTACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTgg                                                                                                        >  2:1996125/1‑149 (MQ=255)
                                               ccccGAAATTCATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGc                                                                                                       <  1:1625367/149‑1 (MQ=255)
                                                   gAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATc                                                                                                   <  2:2246983/149‑1 (MQ=255)
                                                      aTTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAAc                                                                                                <  1:3309351/149‑1 (MQ=255)
                                                       ttAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACt                                                                                               >  2:1040426/1‑149 (MQ=255)
                                                       ttAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACt                                                                                               >  1:500598/1‑149 (MQ=255)
                                                          aTGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTggg                                                                                            <  1:1273982/149‑1 (MQ=255)
                                                          aTGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTggg                                                                                            >  2:1884782/1‑149 (MQ=255)
                                                          aTGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTggg                                                                                            >  1:261205/1‑149 (MQ=255)
                                                           tGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCTACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGa                                                                                           <  2:1584753/149‑1 (MQ=255)
                                                              aGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATAc                                                                                        <  2:473972/149‑1 (MQ=255)
                                                                cAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACgg                                                                                      <  2:32782/149‑1 (MQ=255)
                                                                   tACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGa                                                                                   >  1:4204410/1‑149 (MQ=255)
                                                                    aCGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCTACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAg                                                                                  <  2:627255/149‑1 (MQ=255)
                                                                    aCGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAg                                                                                  <  1:3779720/149‑1 (MQ=255)
                                                                            cTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCTACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCt                                                                          >  2:3433091/1‑149 (MQ=255)
                                                                             tCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTc                                                                         <  2:4267642/149‑1 (MQ=255)
                                                                                ttCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCTACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGAt                                                                      <  2:568863/149‑1 (MQ=255)
                                                                                    gggTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATgcgc                                                                  <  2:2383167/149‑1 (MQ=255)
                                                                                     ggTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCt                                                                 <  2:751458/149‑1 (MQ=255)
                                                                                        aTCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCgg                                                              >  2:2139153/1‑149 (MQ=255)
                                                                                          cccGACCTGGGATTTTGGTGAACTCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTa                                                            <  1:2511900/149‑1 (MQ=255)
                                                                                           ccGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTAt                                                           >  1:2900371/1‑149 (MQ=255)
                                                                                                 tGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCa                                                     >  2:3385672/1‑149 (MQ=255)
                                                                                                           gTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAActc                                           <  2:2534180/149‑1 (MQ=255)
                                                                                                            tGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAActct                                          >  2:2646841/1‑149 (MQ=255)
                                                                                                            tGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAActct                                          >  2:210064/1‑149 (MQ=255)
                                                                                                                aTCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGAc                                      <  1:2623346/149‑1 (MQ=255)
                                                                                                                aTCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGAc                                      <  1:2268003/149‑1 (MQ=255)
                                                                                                                aTCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGAc                                      <  2:527063/149‑1 (MQ=255)
                                                                                                                 tCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGAcc                                     <  2:390084/149‑1 (MQ=255)
                                                                                                                  ccAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCa                                    >  1:455639/1‑149 (MQ=255)
                                                                                                                   cAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCaa                                   >  2:3099315/1‑149 (MQ=255)
                                                                                                                     ggAAGACTGGGAAGCCGTCTGGGATCAGCTCTACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAg                                 >  2:4226695/1‑149 (MQ=255)
                                                                                                                      gAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAgg                                >  2:2343886/1‑149 (MQ=255)
                                                                                                                       aaGACTGGGAAGCCGTCTGGGATCAGCTCTACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGc                               >  2:1682364/1‑149 (MQ=255)
                                                                                                                        aGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCg                              >  2:1654966/1‑149 (MQ=255)
                                                                                                                           cTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACACACTCTCGACCAAAGGCGTGa                           >  2:4054332/1‑149 (MQ=255)
                                                                                                                           cTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGa                           >  1:4285277/1‑149 (MQ=255)
                                                                                                                            tGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGaa                          <  2:811893/149‑1 (MQ=255)
                                                                                                                             gggAAGCCGTCTGGGATCAGCTCTACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAg                         <  2:2836191/149‑1 (MQ=255)
                                                                                                                             gggAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAg                         >  2:1725324/1‑149 (MQ=255)
                                                                                                                             gggAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAg                         <  1:1022685/149‑1 (MQ=255)
                                                                                                                             gggAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAg                         >  1:1248674/1‑149 (MQ=255)
                                                                                                                             gggAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAg                         <  1:673109/149‑1 (MQ=255)
                                                                                                                              ggAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGt                        >  2:3316306/1‑149 (MQ=255)
                                                                                                                               gAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGtt                       <  1:277222/149‑1 (MQ=255)
                                                                                                                                aaGCCGTCTGGGATCAGCTCTACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGAtc                      >  2:3750601/1‑149 (MQ=255)
                                                                                                                                  gCCGTCTGGGATCAGCTCTACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTtcgt                    <  1:1996125/149‑1 (MQ=255)
                                                                                                                                  gCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTtcgt                    <  2:3168750/149‑1 (MQ=255)
                                                                                                                                  gCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTtcgt                    >  1:1464084/1‑149 (MQ=255)
                                                                                                                                  gCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTtcgt                    <  1:3719232/149‑1 (MQ=255)
                                                                                                                                    cGTCTGGGATCAGCTCGACGACCAGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTtcgtcg                  <  2:4381078/149‑1 (MQ=255)
                                                                                                                                      tCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGCGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGc                <  2:1769117/149‑1 (MQ=255)
                                                                                                                                      tCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGc                >  2:291724/1‑149 (MQ=255)
                                                                                                                                      tCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCGGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGc                <  2:3923832/149‑1 (MQ=255)
                                                                                                                                         gggATCAGCTCTACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGCTAc             >  1:4600869/1‑149 (MQ=255)
                                                                                                                                         gggATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGCTAc             <  1:623285/149‑1 (MQ=255)
                                                                                                                                              cAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGCTCCTGGcc        >  1:4494570/1‑149 (MQ=255)
                                                                                                                                              cAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGCTACTGGcc        >  1:4494245/1‑149 (MQ=255)
                                                                                                                                               aGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGCTACTGGCCa       >  1:1584430/1‑149 (MQ=255)
                                                                                                                                               aGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGCTACTGGCCa       <  1:756790/149‑1 (MQ=255)
                                                                                                                                               aGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGCTACTGGCCa       >  1:1145931/1‑149 (MQ=255)
                                                                                                                                               aGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGCTACTGGCCa       <  2:1270283/149‑1 (MQ=255)
                                                                                                                                                gCTCTACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGCTACTGGCCaa      <  2:3552314/149‑1 (MQ=255)
                                                                                                                                                   cgacgaCCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGCTACTGGCCAACgg   <  1:318680/149‑1 (MQ=255)
                                                                                                                                                   cgacgaCCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGCTACTGGCCAACgg   <  1:318513/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TATTATCGGCCCAGAACTGGTGACCTTACATAACCTCAAGGACGACTCCCCGAAATTAATGGAGCAGTACGATGTGCTCATTCTGGGTATCCCGACCTGGGATTTTGGTGAAATCCAGGAAGACTGGGAAGCCGTCTGGGATCAGCTCGACGACCTGAACCTTGAAGGTAAAATTGTTGCGCTGTATGGGCTTGGCGATCAACTGGGATACGGCGAGTGGTTCCTCGATGCGCTCGGTATGCTGCATGACAAACTCTCGACCAAAGGCGTGAAGTTCGTCGGCTACTGGCCAACGGA  >  CP009273/3033282‑3033578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑