BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,116,216	G→T	7.4%	R317S (CGT→AGT)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,116,216	0	G	T	7.4%	1134.3 / 45.6	298	R317S (CGT→AGT)	glcB	malate synthase G
Reads supporting (aligned to +/- strand): ref base G (131/144); new base T (12/11); total (143/155)
Fisher's exact test for biased strand distribution p-value = 8.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 301 total aligned reads displayed.

ATGACGCCATCAAGAATGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATG  >  CP009273/3116068‑3116364

aTGACGCCATCAAGAATGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACg                                                                                                                                                      <  2:2669067/149‑1 (MQ=255)

 tGACGCCATCAAGAATGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGa                                                                                                                                                     <  2:3930854/149‑1 (MQ=255)

 tGACGCCATCAAGAATGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGa                                                                                                                                                     <  1:2136040/149‑1 (MQ=255)

  gACGCCATCAAGAATGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGAt                                                                                                                                                    <  1:3253065/149‑1 (MQ=255)

   aCGCCATCAAGAATGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATc                                                                                                                                                   <  2:2763290/149‑1 (MQ=255)

       cATCAAGAATGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCa                                                                                                                                               <  2:1703966/149‑1 (MQ=255)

        aTCAAGAATGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCAt                                                                                                                                              <  2:2278323/149‑1 (MQ=255)

        aTCAAGAATGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCAt                                                                                                                                              <  2:4222008/149‑1 (MQ=255)

         tCAAGAATGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCAtt                                                                                                                                             >  1:1054828/1‑149 (MQ=255)

           aaGAATGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCa                                                                                                                                           <  1:423152/149‑1 (MQ=255)

           aaGAATGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATGTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCa                                                                                                                                           >  2:4088627/1‑149 (MQ=255)

             gAATGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGt                                                                                                                                         >  2:2343792/1‑149 (MQ=255)

              aaTGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGtt                                                                                                                                        >  2:2078468/1‑149 (MQ=255)

               aTGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGttt                                                                                                                                       <  2:1240359/149‑1 (MQ=255)

               aTGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGttt                                                                                                                                       <  2:1172156/149‑1 (MQ=255)

                tGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTa                                                                                                                                      >  1:1536153/1‑149 (MQ=255)

                tGCCTTCCGGGATTTCATTGCCTTCGCTGTCACAAATCACAGGAATGGTCATCAAATGACACACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTAAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTa                                                                                                                                      >  2:3610456/1‑149 (MQ=255)

                      ccGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACg                                                                                                                                >  2:1769255/1‑149 (MQ=255)

                        gggATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGAt                                                                                                                              <  2:4442813/149‑1 (MQ=255)

                        gggATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGAt                                                                                                                              >  1:910361/1‑149 (MQ=255)

                        gggATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGAt                                                                                                                              <  1:332374/149‑1 (MQ=255)

                         ggATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGAtt                                                                                                                             >  1:647323/1‑149 (MQ=255)

                         ggATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGAtt                                                                                                                             >  2:660955/1‑149 (MQ=255)

                          gATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGAttt                                                                                                                            <  2:340999/149‑1 (MQ=255)

                          gATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGAttt                                                                                                                            >  2:74103/1‑149 (MQ=255)

                           aTTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTg                                                                                                                           >  1:4193272/1‑149 (MQ=255)

                            tttCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGc                                                                                                                          <  1:1457544/149‑1 (MQ=255)

                             ttCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACTATCGTCATTCAGTTTACGCACGATTTGcc                                                                                                                         >  2:2937584/1‑149 (MQ=255)

                                aTTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACTATCGTCATTCAGTTTACGCACGATTTGCCGAc                                                                                                                      <  1:2073496/149‑1 (MQ=255)

                                aTTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACTATCGTCATTCAGTTTACGCACGATTTGCCGAc                                                                                                                      <  1:4572396/149‑1 (MQ=255)

                                   gCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGt                                                                                                                   <  1:3443296/149‑1 (MQ=255)

                                   gCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGt                                                                                                                   <  2:4502962/149‑1 (MQ=255)

                                    ccTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGtt                                                                                                                  >  2:4129956/1‑149 (MQ=255)

                                    ccTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGtt                                                                                                                  >  1:4131542/1‑149 (MQ=255)

                                         gCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTtct                                                                                                             <  1:4380975/149‑1 (MQ=255)

                                           tGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTcc                                                                                                           >  2:3884260/1‑149 (MQ=255)

                                           tGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTcc                                                                                                           <  1:3120243/149‑1 (MQ=255)

                                            gTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCa                                                                                                          >  1:317538/1‑149 (MQ=255)

                                             tCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCAt                                                                                                         <  2:3532001/149‑1 (MQ=255)

                                             tCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCAt                                                                                                         >  2:2226347/1‑149 (MQ=255)

                                             tCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCAt                                                                                                         <  1:799898/149‑1 (MQ=255)

                                              cccAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCAtt                                                                                                        >  1:3601545/1‑149 (MQ=255)

                                                 aaaTCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTtc                                                                                                     >  2:281566/1‑149 (MQ=255)

                                                       caGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGc                                                                                               >  2:3313396/1‑149 (MQ=255)

                                                       caGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGc                                                                                               <  1:379763/149‑1 (MQ=255)

                                                       caGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGc                                                                                               <  1:4660505/149‑1 (MQ=255)

                                                        aGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCa                                                                                              >  1:3968551/1‑149 (MQ=255)

                                                          gAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCaga                                                                                            <  2:3796932/149‑1 (MQ=255)

                                                              ggTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTcc                                                                                        <  1:3675028/149‑1 (MQ=255)

                                                              ggTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTcc                                                                                        >  2:4062324/1‑149 (MQ=255)

                                                               gTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTccc                                                                                       >  2:2008140/1‑149 (MQ=255)

                                                                tcatcaAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTcccc                                                                                      <  1:3750691/149‑1 (MQ=255)

                                                                tcatcaAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTcccc                                                                                      <  1:1438393/149‑1 (MQ=255)

                                                                   tcaAATGACCCACGGTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGc                                                                                   <  2:4529886/149‑1 (MQ=255)

                                                                    caAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCa                                                                                  >  1:2549483/1‑149 (MQ=255)

                                                                     aaaTGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCAt                                                                                 >  2:1522157/1‑149 (MQ=255)

                                                                       aTGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCa                                                                               >  1:3584821/1‑149 (MQ=255)

                                                                       aTGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCa                                                                               >  1:3452855/1‑149 (MQ=255)

                                                                          aCCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGc                                                                            <  1:1319162/149‑1 (MQ=255)

                                                                            ccACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGccc                                                                          <  1:2390872/149‑1 (MQ=255)

                                                                                      gATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGc                                                                >  1:151627/1‑149 (MQ=255)

                                                                                       aTAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCg                                                               >  1:4525121/1‑149 (MQ=255)

                                                                                          aaCAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTa                                                            <  1:219308/149‑1 (MQ=255)

                                                                                          aaCAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTa                                                            <  1:2689666/149‑1 (MQ=255)

                                                                                           aCAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTAc                                                           <  2:2014073/149‑1 (MQ=255)

                                                                                            cagcagCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTAca                                                          <  2:1158865/149‑1 (MQ=255)

                                                                                             agcagcGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTAcag                                                         >  1:1611548/1‑149 (MQ=255)

                                                                                                   gagcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACTATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGAt                                                   <  2:1833762/149‑1 (MQ=255)

                                                                                                   gagcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACTATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGAt                                                   <  1:2937584/149‑1 (MQ=255)

                                                                                                   gagcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGAt                                                   <  1:896458/149‑1 (MQ=255)

                                                                                                   gagcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGAt                                                   >  1:2042278/1‑149 (MQ=255)

                                                                                                     gcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGAttt                                                 <  2:1025585/149‑1 (MQ=255)

                                                                                                     gcgTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGAttt                                                 >  1:4277412/1‑149 (MQ=255)

                                                                                                        tCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTAt                                              >  2:2054786/1‑149 (MQ=255)

                                                                                                          cGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCt                                            <  1:1896175/149‑1 (MQ=255)

                                                                                                           gTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCtt                                           <  2:1803859/149‑1 (MQ=255)

                                                                                                           gTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTCCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCtt                                           >  1:3522257/1‑149 (MQ=255)

                                                                                                            tGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACTATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTc                                          <  2:1602735/149‑1 (MQ=255)

                                                                                                            tGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTc                                          <  2:1900998/149‑1 (MQ=255)

                                                                                                            tGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTc                                          <  2:3379790/149‑1 (MQ=255)

                                                                                                            tGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTc                                          <  2:2111362/149‑1 (MQ=255)

                                                                                                               agagAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGc                                       >  1:476940/1‑149 (MQ=255)

                                                                                                                gagaAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCa                                      <  2:2720774/149‑1 (MQ=255)

                                                                                                                gagaAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCa                                      <  2:2573908/149‑1 (MQ=255)

                                                                                                                  gaAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATc                                    <  2:1290801/149‑1 (MQ=255)

                                                                                                                    aaTTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCaa                                  >  1:918209/1‑149 (MQ=255)

                                                                                                                      tttCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAAcc                                <  1:2613554/149‑1 (MQ=255)

                                                                                                                      tttCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAAcc                                <  2:3005674/149‑1 (MQ=255)

                                                                                                                         cAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAAccgcc                             <  2:524057/149‑1 (MQ=255)

                                                                                                                              ccATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGAc                        <  2:3426175/149‑1 (MQ=255)

                                                                                                                                aTCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCg                      >  1:4440545/1‑149 (MQ=255)

                                                                                                                                aTCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCg                      <  2:2192832/149‑1 (MQ=255)

                                                                                                                                 tCGGCGGCGGTGTAATGACTATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGa                     >  1:3480775/1‑149 (MQ=255)

                                                                                                                                 tCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGa                     >  1:8662/1‑149 (MQ=255)

                                                                                                                                  cggcggcggTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTACATTTTCTCTTGCATAGTCCCCTTCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATATTCCGCATCAACCGCCGCGACCGaa                    >  1:3309583/1‑149 (MQ=255)

                                                                                                                                   ggcggcggTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAAt                   <  1:3027273/149‑1 (MQ=255)

                                                                                                                                   ggcggcggTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAAt                   <  2:4260739/149‑1 (MQ=255)

                                                                                                                                     cggcggTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCt                 <  2:4423275/149‑1 (MQ=255)

                                                                                                                                         ggTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGc             >  1:4263670/1‑149 (MQ=255)

                                                                                                                                          gtgtAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCa            >  2:4619369/1‑149 (MQ=255)

ATGACGCCATCAAGAATGCCTTCCGGGATTTCATTGCCTTCGCTGTCCCAAATCACAGGAATGGTCATCAAATGACCCACGTTGCGGATAAACAGCAGCGAGCGTCCGTGCAGAGAAATTTCAGAGCCATCGGCGGCGGTGTAATGACGATCGTCATTCAGTTTACGCACGATTTGCCGACCGTTTTTCTCCATTTTCTCTTGCAGAGTCCCCTGCATCAGGCCCAGCAGGTTGCGGTACAGCAGGATTTTATCTTCCGCATCAACCGCCGCGACCGAATCTTCGCAGTCGAGAATG  >  CP009273/3116068‑3116364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`