Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,131,302 C→A 5.5% D194Y (GAT→TAT)  gss ← fused glutathionylspermidine amidase/glutathionylspermidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,131,3020CA 5.5% 1116.8 / 29.7 288D194Y (GAT→TAT) gssfused glutathionylspermidine amidase/glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base C (134/137);  new base A (8/9);  total (142/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

TGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCACGTTCTGTTCCGCAATA  >  CP009273/3131154‑3131450
                                                                                                                                                    |                                                                                                                                                    
tGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATc                                                                                                                                                      <  2:1899496/149‑1 (MQ=255)
  aCCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCtt                                                                                                                                                    >  2:2649771/1‑149 (MQ=255)
   ccGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTc                                                                                                                                                   >  2:3088050/1‑149 (MQ=255)
   ccGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATATTc                                                                                                                                                   <  1:3327049/149‑1 (MQ=255)
        ggCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCt                                                                                                                                              >  1:3355604/1‑149 (MQ=255)
         gCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTg                                                                                                                                             >  2:4313130/1‑149 (MQ=255)
            tGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGat                                                                                                                                          >  1:133423/1‑149 (MQ=255)
               acaTAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGatcat                                                                                                                                       >  1:3232431/1‑149 (MQ=255)
                 aTAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATcc                                                                                                                                     >  2:1646124/1‑149 (MQ=255)
                 aTAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATcc                                                                                                                                     <  2:61557/149‑1 (MQ=255)
                     gCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGcc                                                                                                                                 <  1:3628544/149‑1 (MQ=255)
                     gCGTTTTGCAGCGGATCCTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGcc                                                                                                                                 <  2:3026149/149‑1 (MQ=255)
                      cGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGccc                                                                                                                                <  1:106055/149‑1 (MQ=255)
                      cGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGccc                                                                                                                                <  1:106040/149‑1 (MQ=255)
                       gTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCa                                                                                                                               <  1:913812/149‑1 (MQ=255)
                       gTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCa                                                                                                                               >  1:1365934/1‑149 (MQ=255)
                       gTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCa                                                                                                                               >  1:3792895/1‑149 (MQ=255)
                       gTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCa                                                                                                                               >  1:3703050/1‑149 (MQ=255)
                         tttGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGa                                                                                                                             <  2:3808718/149‑1 (MQ=255)
                             cAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAAtgg                                                                                                                         <  2:1386978/149‑1 (MQ=255)
                             cAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAAtgg                                                                                                                         <  2:3570352/149‑1 (MQ=255)
                              aGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAAtggt                                                                                                                        <  1:2438069/149‑1 (MQ=255)
                              aGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAAtggt                                                                                                                        <  1:4045343/149‑1 (MQ=255)
                               gCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAAtggtg                                                                                                                       >  2:2117035/1‑149 (MQ=255)
                                 ggATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATggtggt                                                                                                                     >  2:1034173/1‑149 (MQ=255)
                                 ggATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATggtggt                                                                                                                     <  2:2618234/149‑1 (MQ=255)
                                 ggATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATggtggt                                                                                                                     >  2:4299344/1‑149 (MQ=255)
                                  gATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGgtggtg                                                                                                                    >  2:1475512/1‑149 (MQ=255)
                                   aTCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGgtgt                                                                                                                   >  1:1396806/1‑149 (MQ=255)
                                   aTCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGgtgt                                                                                                                   <  2:1810387/149‑1 (MQ=255)
                                    tCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCTGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGtc                                                                                                                  <  2:612323/149‑1 (MQ=255)
                                    tCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGtc                                                                                                                  <  2:687347/149‑1 (MQ=255)
                                    tCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGtc                                                                                                                  >  2:4668322/1‑149 (MQ=255)
                                     cTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGtca                                                                                                                 <  2:717860/149‑1 (MQ=255)
                                      tttttCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGtcat                                                                                                                <  2:3995680/149‑1 (MQ=255)
                                         ttCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATATTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCaa                                                                                                             >  2:52983/1‑149 (MQ=255)
                                            aTCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAg                                                                                                          >  1:1595540/1‑149 (MQ=255)
                                            aTCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAg                                                                                                          <  2:1769652/149‑1 (MQ=255)
                                             tCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAgt                                                                                                         >  2:2264293/1‑149 (MQ=255)
                                               cAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAgtgt                                                                                                       <  2:1684536/149‑1 (MQ=255)
                                                aGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTc                                                                                                      <  1:4115226/149‑1 (MQ=255)
                                                    aTTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTc                                                                                                  >  2:82780/1‑149 (MQ=255)
                                                         ccGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGt                                                                                             >  1:3005209/1‑149 (MQ=255)
                                                           gTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGtat                                                                                           <  2:2551807/149‑1 (MQ=255)
                                                            tCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGtata                                                                                          >  2:422759/1‑149 (MQ=255)
                                                             cAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATag                                                                                         <  1:2210726/149‑1 (MQ=255)
                                                                 cTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATagcagc                                                                                     >  2:4146459/1‑149 (MQ=255)
                                                                   ggCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCg                                                                                   >  2:4048755/1‑149 (MQ=255)
                                                                     ccTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGtt                                                                                 <  2:1190021/149‑1 (MQ=255)
                                                                     ccTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGtt                                                                                 >  1:3970492/1‑149 (MQ=255)
                                                                     ccTTGGTGTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGtt                                                                                 <  1:2668685/149‑1 (MQ=255)
                                                                       tttGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGtttt                                                                               <  1:4075031/149‑1 (MQ=255)
                                                                              tCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGaccac                                                                        >  2:2931256/1‑149 (MQ=255)
                                                                                cAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGAccacca                                                                      >  2:2418474/1‑149 (MQ=255)
                                                                                cAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATATTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGAccacca                                                                      >  1:2619524/1‑149 (MQ=255)
                                                                                   gcgcgcTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCACTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCAtct                                                                   >  1:1661280/1‑149 (MQ=255)
                                                                                     gcgcTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTcc                                                                 >  1:4317680/1‑149 (MQ=255)
                                                                                     gcgcTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTcc                                                                 <  2:3521893/149‑1 (MQ=255)
                                                                                            gCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTcgc                                                          <  1:82780/149‑1 (MQ=255)
                                                                                               gATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTcgcgcg                                                       >  1:206661/1‑149 (MQ=255)
                                                                                               gATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTcgcgcg                                                       >  1:3589054/1‑149 (MQ=255)
                                                                                                 ttttCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTc                                                     <  2:3761097/149‑1 (MQ=255)
                                                                                                  tttCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTcc                                                    <  2:1967723/149‑1 (MQ=255)
                                                                                                  tttCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTcc                                                    >  1:728771/1‑149 (MQ=255)
                                                                                                     cagcagCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACt                                                 <  2:4533073/149‑1 (MQ=255)
                                                                                                          gcTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGc                                            <  2:3830112/149‑1 (MQ=255)
                                                                                                             cGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCt                                         >  1:48923/1‑149 (MQ=255)
                                                                                                              gCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCtt                                        <  1:4439408/149‑1 (MQ=255)
                                                                                                              gCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCta                                        >  2:3659152/1‑148 (MQ=255)
                                                                                                                cTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATATTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGc                                      <  1:2313816/149‑1 (MQ=255)
                                                                                                                 tGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCTGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCg                                     >  1:4219521/1‑149 (MQ=255)
                                                                                                                 tGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCg                                     >  1:1689058/1‑149 (MQ=255)
                                                                                                                  gCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCgg                                    >  2:1733792/1‑149 (MQ=255)
                                                                                                                     aTTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCCCCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCaa                                 <  2:442609/149‑1 (MQ=255)
                                                                                                                     aTTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCaa                                 >  1:3160319/1‑149 (MQ=255)
                                                                                                                     aTTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCaa                                 >  1:4228992/1‑149 (MQ=255)
                                                                                                                     aTTTCCGGCTGCGGTAAGCTGTATTCAGTATCGTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCaa                                 <  2:3222737/149‑1 (MQ=255)
                                                                                                                      tttCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAAc                                <  1:1224373/149‑1 (MQ=255)
                                                                                                                      tttCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAAc                                <  1:1224529/149‑1 (MQ=255)
                                                                                                                        tCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACgg                              >  1:4586369/1‑149 (MQ=255)
                                                                                                                             cTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGgaat                         <  1:224821/149‑1 (MQ=255)
                                                                                                                              tGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGgaatg                        >  2:1034981/1‑149 (MQ=255)
                                                                                                                                   tAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCa                   >  2:4412777/1‑149 (MQ=255)
                                                                                                                                   tAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCa                   >  2:3256934/1‑149 (MQ=255)
                                                                                                                                   tAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCa                   <  1:3113634/149‑1 (MQ=255)
                                                                                                                                    aaGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCAc                  >  2:2764870/1‑149 (MQ=255)
                                                                                                                                    aaGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGGCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCAc                  <  1:1820245/149‑1 (MQ=255)
                                                                                                                                     aGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCACg                 <  2:3464419/149‑1 (MQ=255)
                                                                                                                                     aGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCACg                 <  2:2089474/149‑1 (MQ=255)
                                                                                                                                       cTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCACCGGGGAATGAATCACggt               >  2:2297644/1‑147 (MQ=255)
                                                                                                                                       cTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCACGtt               >  2:4010342/1‑149 (MQ=255)
                                                                                                                                        tGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCACGTTc              <  1:1991206/149‑1 (MQ=255)
                                                                                                                                        tGTATTCAGTATATTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCACGTTc              <  1:2528712/149‑1 (MQ=255)
                                                                                                                                         gTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCACGTTCt             >  1:3863613/1‑149 (MQ=255)
                                                                                                                                         gTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACGGTTGCCCTTGCGGCAACGGGGAATGAATCACGTTCt             <  1:4133543/149‑1 (MQ=255)
                                                                                                                                         gTATTCAGTATCTTCCGTCTGGATCACCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCACGTTCt             >  2:3130042/1‑149 (MQ=255)
                                                                                                                                                  aTCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCACGTTCTGTTCCGCaa    >  1:2958024/1‑149 (MQ=255)
                                                                                                                                                  aTCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCACGTTCTGTTCCGCaa    >  1:1985967/1‑149 (MQ=255)
                                                                                                                                                   tCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCACGTTCTGTTCCGCAAt   <  2:938142/149‑1 (MQ=255)
                                                                                                                                                    cTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGACTGAATCACGTTCTGTTCCGCAATa  >  1:4571742/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGACCGTTGGCCTGCACATAGGCGTTTTGCAGCGGATCTTTTTCATCCAGCCATTTACCGTCAAACTGGCCTTTGTTTTCCAGGCGCGCTCCGCTGATTTTCAGCAGCTCGCCTGCAATTTCCGGCTGCGGTAAGCTGTATTCAGTATCTTCCGTCTGGATCATCCAGCCCAGAATGGTGGTGTCATCAAAAGTGTCTTTCAGGGTATAGCAGCCGTTTTCGACCACCATCTCCAGCTCGCGCGTCCACTGTTGCCCTTGCGGCAACGGGGAATGAATCACGTTCTGTTCCGCAATA  >  CP009273/3131154‑3131450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑