BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,173,669	G→T	65.0%	A189A (GCG→GCT)	ygiB →	DUF1190 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,173,669	0	G	T	65.0%	244.4 / inf	269	A189A (GCG→GCT)	ygiB	DUF1190 family protein
Reads supporting (aligned to +/- strand): ref base G (47/47); new base T (89/86); total (136/133)
Fisher's exact test for biased strand distribution p-value = 8.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

Only 100 of 271 total aligned reads displayed.

GATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTG  >  CP009273/3173521‑3173814

gATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCg                                                                                                                                                   <  2:1028191/149‑1 (MQ=255)

   ttGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCCCCAAAACCGGCGacc                                                                                                                                                >  1:1984751/1‑149 (MQ=255)

       acaGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTAccacca                                                                                                                                            >  1:3386932/1‑149 (MQ=255)

         agcagcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACt                                                                                                                                          >  2:1468120/1‑149 (MQ=255)

           cagcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTAc                                                                                                                                        <  1:3031011/149‑1 (MQ=255)

            agcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGACACCAAAACCGGCGACCACCACTAcc                                                                                                                                       >  2:3866665/1‑149 (MQ=255)

              ccGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGt                                                                                                                                     >  2:853623/1‑149 (MQ=255)

                gCTGTTCTCCTCGAAAAACCCAGCCAGACCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTa                                                                                                                                   >  2:3730398/1‑149 (MQ=255)

                  tGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTAcc                                                                                                                                 >  1:136546/1‑149 (MQ=255)

                    ttCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCg                                                                                                                               >  2:217641/1‑149 (MQ=255)

                       tcctcGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTCAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTgg                                                                                                                            >  1:2665633/1‑149 (MQ=255)

                        cctcGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGc                                                                                                                           >  1:1102119/1‑149 (MQ=255)

                             aaaaaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGttt                                                                                                                      <  2:227254/149‑1 (MQ=255)

                             aaaaaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGttt                                                                                                                      >  2:3371787/1‑149 (MQ=255)

                              aaaaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGtttt                                                                                                                     >  2:4078453/1‑149 (MQ=255)

                               aaaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTg                                                                                                                    >  1:4573120/1‑149 (MQ=255)

                                aaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTgg                                                                                                                   >  2:2650834/1‑149 (MQ=255)

                                   ccagccagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGa                                                                                                                >  1:2885451/1‑149 (MQ=255)

                                   ccagccagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGa                                                                                                                >  1:545314/1‑149 (MQ=255)

                                    cagccagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGaa                                                                                                               <  2:2308731/149‑1 (MQ=255)

                                    cagccagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGaa                                                                                                               <  2:1132631/149‑1 (MQ=255)

                                     agccagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGATCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAAt                                                                                                              >  1:1475331/1‑149 (MQ=255)

                                         agTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGt                                                                                                          <  1:2310450/149‑1 (MQ=255)

                                          gTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGtt                                                                                                         >  2:3224167/1‑149 (MQ=255)

                                           tCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTg                                                                                                        >  1:2195521/1‑149 (MQ=255)

                                             cGGCTTACGGTAAAGATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGcc                                                                                                      <  2:940736/149‑1 (MQ=255)

                                               gCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaa                                                                                                    >  2:4519491/1‑149 (MQ=255)

                                                cTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaa                                                                                                   <  2:2491849/149‑1 (MQ=255)

                                                cTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaa                                                                                                   >  1:2683968/1‑149 (MQ=255)

                                                 ttACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaac                                                                                                  >  1:3747549/1‑149 (MQ=255)

                                                     ggTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAg                                                                                              >  2:3628540/1‑149 (MQ=255)

                                                     ggTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAg                                                                                              <  1:4283473/149‑1 (MQ=255)

                                                      gTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTAACCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGc                                                                                             >  1:1894030/1‑149 (MQ=255)

                                                      gTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGc                                                                                             <  2:469517/149‑1 (MQ=255)

                                                           tataCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTAt                                                                                        <  1:4519027/149‑1 (MQ=255)

                                                           tataCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTAACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTAt                                                                                        >  2:3428461/1‑149 (MQ=255)

                                                            ataCCGACGCGACGGGTACAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATg                                                                                       >  1:4302894/1‑149 (MQ=255)

                                                            ataCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATg                                                                                       >  1:4394394/1‑149 (MQ=255)

                                                              aCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCa                                                                                     <  2:4530967/149‑1 (MQ=255)

                                                                cgacgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGc                                                                                   <  2:1306106/149‑1 (MQ=255)

                                                                 gacgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCg                                                                                  <  1:4112444/149‑1 (MQ=255)

                                                                   cgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTa                                                                                <  2:3030398/149‑1 (MQ=255)

                                                                   cgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTa                                                                                <  2:690460/149‑1 (MQ=255)

                                                                    gcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAg                                                                               <  2:1502740/149‑1 (MQ=255)

                                                                       acgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGc                                                                            <  2:3555339/149‑1 (MQ=255)

                                                                        cgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCa                                                                           >  2:377105/1‑149 (MQ=255)

                                                                         gggTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCaa                                                                          <  2:174126/149‑1 (MQ=255)

                                                                          ggTAAAAACTCTGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAAc                                                                         <  2:819835/149‑1 (MQ=255)

                                                                          ggTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAAc                                                                         <  2:2230574/149‑1 (MQ=255)

                                                                          ggTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAAc                                                                         <  1:1668707/149‑1 (MQ=255)

                                                                              aaaaCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGt                                                                     <  2:3969218/149‑1 (MQ=255)

                                                                              aaaaCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGt                                                                     >  1:2431824/1‑149 (MQ=255)

                                                                              aaaaCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGt                                                                     >  2:4146167/1‑149 (MQ=255)

                                                                                aaCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAc                                                                   <  2:4281929/149‑1 (MQ=255)

                                                                                 aCTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAcc                                                                  <  2:588886/149‑1 (MQ=255)

                                                                                   tATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACctc                                                                >  1:4678335/1‑149 (MQ=255)

                                                                                        cgcAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTtctc                                                           <  2:550273/149‑1 (MQ=255)

                                                                                           agcccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGtt                                                        <  2:16514/149‑1 (MQ=255)

                                                                                            gcccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTc                                                       >  2:2021507/1‑149 (MQ=255)

                                                                                            gcccagccAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTc                                                       >  1:3438450/1‑149 (MQ=255)

                                                                                                 gccagGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATgg                                                  >  1:3358542/1‑149 (MQ=255)

                                                                                                   cagGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGt                                                >  1:1177964/1‑149 (MQ=255)

                                                                                                         gCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGa                                          <  2:2267120/149‑1 (MQ=255)

                                                                                                           aCCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATa                                        <  1:2424652/149‑1 (MQ=255)

                                                                                                           aCCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATa                                        >  1:717246/1‑149 (MQ=255)

                                                                                                            ccATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATAc                                       <  2:1600783/149‑1 (MQ=255)

                                                                                                             cATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATAcc                                      >  1:4654967/1‑149 (MQ=255)

                                                                                                               tGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGa                                    <  2:244846/149‑1 (MQ=255)

                                                                                                                gACCGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGAt                                   <  1:3705708/149‑1 (MQ=255)

                                                                                                                 aCCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATg                                  >  1:2711095/1‑149 (MQ=255)

                                                                                                                   cGTACCGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGa                                <  1:1755889/149‑1 (MQ=255)

                                                                                                                    gTACCGAAGACGGCAATGGCACCAACACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGaa                               <  2:4255030/149‑1 (MQ=255)

                                                                                                                       ccGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAaga                            <  1:230059/149‑1 (MQ=255)

                                                                                                                       ccGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTGTTGGTGAATCTGTTGCCAAACAAAGCACTATTCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAAAGGGTGGCTGATACCGATGGAAaga                            >  1:1255146/1‑149 (MQ=255)

                                                                                                                        cGAAGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAagag                           <  2:3806153/149‑1 (MQ=255)

                                                                                                                         gAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCAGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACATCATCTCGTTCAATGGGTGGCTGATAACGATGGAAAGAGt                          >  2:2724322/1‑149 (MQ=255)

                                                                                                                          aaGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTc                         >  1:3799947/1‑149 (MQ=255)

                                                                                                                          aaGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTc                         >  1:364173/1‑149 (MQ=255)

                                                                                                                           aGACGGCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCa                        >  1:4056290/1‑149 (MQ=255)

                                                                                                                               ggCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTAt                    <  1:648659/149‑1 (MQ=255)

                                                                                                                                gCAATGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTAtt                   <  2:2941993/149‑1 (MQ=255)

                                                                                                                                  aaTGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTAc                 >  2:4609503/1‑149 (MQ=255)

                                                                                                                                   aTGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTAcc                >  2:1866265/1‑149 (MQ=255)

                                                                                                                                   aTGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTAcc                <  1:853623/149‑1 (MQ=255)

                                                                                                                                    tGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCg               >  2:996656/1‑149 (MQ=255)

                                                                                                                                    tGGCACCAAAACCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCg               >  2:3332585/1‑149 (MQ=255)

                                                                                                                                     ggCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGa              <  1:3932347/149‑1 (MQ=255)

                                                                                                                                     ggCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGa              <  2:2356045/149‑1 (MQ=255)

                                                                                                                                          cAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGATCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGcc         >  1:3121574/1‑149 (MQ=255)

                                                                                                                                           aaaaCCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGccc        >  2:1912749/1‑149 (MQ=255)

                                                                                                                                           aaaaCCGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGccc        <  1:225777/149‑1 (MQ=255)

                                                                                                                                               ccGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGAc    <  2:2888668/149‑1 (MQ=255)

                                                                                                                                               ccGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGAc    <  2:3444733/149‑1 (MQ=255)

                                                                                                                                                cGGCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACt   <  2:2554876/149‑1 (MQ=255)

                                                                                                                                                cGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACt   <  1:2979369/149‑1 (MQ=255)

                                                                                                                                                cGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACt   <  2:629030/149‑1 (MQ=255)

                                                                                                                                                cGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACt   <  2:2983340/149‑1 (MQ=255)

                                                                                                                                                 ggCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTg  >  1:969361/1‑149 (MQ=255)

                                                                                                                                                 ggCTACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTg  >  1:3260070/1‑149 (MQ=255)

                                                                                                                                                 ggCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTg  >  2:3300609/1‑149 (MQ=255)

GATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTG  >  CP009273/3173521‑3173814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`