Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,455,867 C→A 86.5% I55I (ATC→ATA)  gspJ → putative general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,455,8670CA86.5% 680.3 / 98.3 247I55I (ATC→ATA) gspJputative general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base C (21/13);  new base A (102/111);  total (123/124)
Fisher's exact test for biased strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

GTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACT  >  CP009273/3455722‑3456014
                                                                                                                                                 |                                                                                                                                                   
 tttCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGcaccataacca                                                                                                                                                 <  2:624578/149‑1 (MQ=255)
 tttCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGcaccataacca                                                                                                                                                 <  2:3944675/149‑1 (MQ=255)
  ttCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGcaccataaccat                                                                                                                                                >  2:154243/1‑149 (MQ=255)
  ttCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGcaccataaccat                                                                                                                                                <  1:3158261/149‑1 (MQ=255)
   tCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCAtt                                                                                                                                               <  1:563607/149‑1 (MQ=255)
     acaTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCt                                                                                                                                             <  1:2501160/149‑1 (MQ=255)
      caTTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAAAGCTCACAGAAAGAAATACAGCAGTTCAATCATCTACAGCGCACCATAACCAATCtt                                                                                                                                            >  2:3615118/1‑149 (MQ=255)
        ttGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTTTGATTTTTTAGCAAGCTTCCGAGTTGCATCAAAGCACACAGAAAGAAATACAGCAGTTCAAACAGCTAAAGCGCACCAAAACCAATCTTGa                                                                                                                                          >  2:1611794/1‑149 (MQ=255)
        ttGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGa                                                                                                                                          >  2:1921799/1‑149 (MQ=255)
         tGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGAt                                                                                                                                         <  1:4118083/149‑1 (MQ=255)
         tGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGAt                                                                                                                                         <  1:4650279/149‑1 (MQ=255)
           cTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATaa                                                                                                                                       <  2:852733/149‑1 (MQ=255)
           cTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATaa                                                                                                                                       <  2:3188583/149‑1 (MQ=255)
            tGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCCGCAGTTCAATCAGAGCCAGCGCACCATAACCATTCTTGATAAc                                                                                                                                      >  2:4593949/1‑149 (MQ=255)
             ggAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTTTGATTTTTTAGCAAGCTTCCGAGTTGCATCAAAGCTCACCGAAAGAAATACAGCAGTTAAATCAGCTACAGCGCACCCAAACCAATATTGATaaag                                                                                                                                     >  1:643375/1‑147 (MQ=255)
             ggAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCACTTCAAACAGCTACAGCGCACCATCACCAATCTTGATAACg                                                                                                                                     >  2:1357291/1‑149 (MQ=255)
              gAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGa                                                                                                                                    <  1:1171989/149‑1 (MQ=255)
                ggTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGAtc                                                                                                                                  <  1:1517053/149‑1 (MQ=255)
                    aTGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCAGCAACGCTCACAGCAAGAAATGCAGCAGTTCAATCAGCTACAGCGCACCCTAACCATTCTTGATAACGATCACCt                                                                                                                              >  2:4345073/1‑149 (MQ=255)
                          gcgcTGGCGATTTTCTCAATGCTTAGCGTGCTTGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACt                                                                                                                        >  1:2568061/1‑149 (MQ=255)
                              tGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTGTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTc                                                                                                                    >  2:3295172/1‑149 (MQ=255)
                              tGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTGTATGATTTTTTAGCAAGCATCCGAGTTTCAACAAAGCACACAGAAAAAAAAACACCAGTTCAAAAAGCTACAGCGCACCAAAAACAATCATTATAAAGATCTCCAGCAACAGGta                                                                                                                    >  1:3743946/1‑148 (MQ=255)
                               ggCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCg                                                                                                                   <  2:2786080/149‑1 (MQ=255)
                                gCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGTTTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGc                                                                                                                  >  2:3337584/1‑149 (MQ=255)
                                gCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGc                                                                                                                  >  2:1001512/1‑149 (MQ=255)
                                gCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGc                                                                                                                  <  2:2397464/149‑1 (MQ=255)
                                gCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATAAGCAACAGCGCACCAACACCATTATTGATAACGATATCCAGCAACTGGTCGc                                                                                                                  >  2:4413984/1‑149 (MQ=255)
                                 cGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGcc                                                                                                                 <  2:4107575/149‑1 (MQ=255)
                                  gATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTACGAGTTTCATAAAAGCTCACAGAAAGAAATACAGCAGTTCAATAAGCTACAGCGCACCAAAACCAATCATTATAAAGAAATCATGCAACAGGTCGccc                                                                                                                >  1:4514608/1‑149 (MQ=255)
                                    ttttCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCgg                                                                                                              >  2:2650128/1‑149 (MQ=255)
                                    ttttCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCgg                                                                                                              >  2:2937369/1‑149 (MQ=255)
                                     tttCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATACAGCAGGTCAATCAGCTACAGCGCACCAACACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCcga                                                                                                             >  1:3316344/1‑149 (MQ=255)
                                     tgtctcAATGCTTAGCGTGCTGGCGTTTATGATTTTGTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCgga                                                                                                             <  2:4380067/147‑1 (MQ=255)
                                          cAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAAt                                                                                                        <  1:3227006/149‑1 (MQ=255)
                                          cAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAAt                                                                                                        <  1:3226679/149‑1 (MQ=255)
                                             tGCTTAGCGTGCTGGCGTTTATTATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCACACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAAACATTCTTGATAACGATCTCCTGCAAATTGTCGCCCCGAGGAATCgg                                                                                                     >  1:1918723/1‑149 (MQ=255)
                                             tGCTTAGCGTGCTGGCGTTTATGATTTTTTGGCAAGCTTCCGAGTTGCATCAACGCTAACAGAAAGAAATCCAGCAGTTAAATCAGCTACAGCGCACCCTAACCAATATTGATAACGATCACCTGCAACTGGTAGCCCGGAGGAATCgg                                                                                                     >  2:822176/1‑149 (MQ=255)
                                                ttAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGt                                                                                                  <  2:942717/149‑1 (MQ=255)
                                                  aGCGTGCTGGCGTTTATTATTTTTTAGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTAAACCGCACCAAAACCATTATTGATAACGATCTCCAGCAAAAGTTCGCCCCGAGGAATCGGAGTAc                                                                                                >  2:1230330/1‑149 (MQ=255)
                                                  aGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTAc                                                                                                >  2:3002862/1‑149 (MQ=255)
                                                  aGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTAc                                                                                                <  2:1456220/149‑1 (MQ=255)
                                                   gCGTGCTGGCGTTTATGATTTTTTAGCAAGCATCCGAGTTGCATCAACCCACACAGAAAGAAAAACAGCAGTTCAAACAGCGACAGCGCACCAAAACCAATCATGATAACCATCTCCAGCAACAGGTCGCCCGGCGGAATCGGCGTACg                                                                                               >  1:3691096/1‑149 (MQ=255)
                                                     gTGCTGGCGTTTATGATTTTTTCGCAATCATACGAGTTTCATCAACCCTCACAGAAAGAAAAACACCAGTTCAATAATCTAAAGCGCACCAAAACCAATCTTGATAAACATATCCAGCAACAGGTCGCCCGGAGGAAAAGGAGTACGGa                                                                                             >  1:4265730/1‑149 (MQ=255)
                                                     gTGCTGGCGTTTATGATTTTGTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGa                                                                                             <  2:2355993/149‑1 (MQ=255)
                                                       gCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCAAAAACAATCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGACTCGGAGTACGGACa                                                                                           >  2:1894019/1‑149 (MQ=255)
                                                        cTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaa                                                                                          <  1:3390168/149‑1 (MQ=255)
                                                          ggCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaaaa                                                                                        <  2:3525076/149‑1 (MQ=255)
                                                          ggCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACaaaa                                                                                        <  1:1020528/149‑1 (MQ=255)
                                                             gTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCa                                                                                     <  2:2023038/149‑1 (MQ=255)
                                                               ttATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATg                                                                                   >  2:3961508/1‑149 (MQ=255)
                                                               ttATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATg                                                                                   >  1:29778/1‑149 (MQ=255)
                                                                tATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATgg                                                                                  <  2:64963/149‑1 (MQ=255)
                                                                   gATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGgtgt                                                                               >  1:789286/1‑149 (MQ=255)
                                                                    aTTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGtt                                                                              <  1:1611794/149‑1 (MQ=255)
                                                                     ttttttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTg                                                                             >  2:4162468/1‑149 (MQ=255)
                                                                     ttttttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTg                                                                             <  1:2232682/149‑1 (MQ=255)
                                                                     ttttttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTg                                                                             >  1:861608/1‑149 (MQ=255)
                                                                      tttttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTgg                                                                            <  1:3916435/149‑1 (MQ=255)
                                                                      tttttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTgg                                                                            >  1:2654470/1‑149 (MQ=255)
                                                                         ttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTg                                                                         >  2:2640157/1‑149 (MQ=255)
                                                                         ttCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTCCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTg                                                                         <  2:1096885/149‑1 (MQ=255)
                                                                          tCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTga                                                                        >  1:4383499/1‑149 (MQ=255)
                                                                            gCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTgaag                                                                      <  1:310303/149‑1 (MQ=255)
                                                                              aaGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGACTCGGAGTACGGACAAAATCATGGTGTTGGGTgaagaa                                                                    >  1:2360456/1‑149 (MQ=255)
                                                                               aGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGaagaag                                                                   >  2:3134326/1‑149 (MQ=255)
                                                                               aGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGaagaag                                                                   >  1:3420919/1‑149 (MQ=255)
                                                                               aGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGaagaag                                                                   >  1:1824128/1‑149 (MQ=255)
                                                                                gCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATGGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGc                                                                  <  2:2244275/149‑1 (MQ=255)
                                                                                gCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGc                                                                  <  1:2255150/149‑1 (MQ=255)
                                                                                gCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGc                                                                  <  2:1749503/149‑1 (MQ=255)
                                                                                  ttCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTa                                                                >  1:875806/1‑149 (MQ=255)
                                                                                  ttCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTa                                                                >  1:4396515/1‑149 (MQ=255)
                                                                                  ttCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTa                                                                >  1:1870309/1‑149 (MQ=255)
                                                                                  ttCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTa                                                                <  2:780127/149‑1 (MQ=255)
                                                                                    ccGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTAtt                                                              <  2:448772/149‑1 (MQ=255)
                                                                                    ccGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTAtt                                                              <  2:3785636/149‑1 (MQ=255)
                                                                                     cGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTAttt                                                             <  1:1828235/149‑1 (MQ=255)
                                                                                       aGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTAttttt                                                           >  1:273043/1‑149 (MQ=255)
                                                                                           gCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCa                                                       >  1:3571495/1‑149 (MQ=255)
                                                                                              tCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGc                                                    <  2:2377508/149‑1 (MQ=255)
                                                                                                  cGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAg                                                <  1:4624632/149‑1 (MQ=255)
                                                                                                     tCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGACGAAGCTATTTTTACCACGCAAAGTcg                                             >  1:2755872/1‑149 (MQ=255)
                                                                                                      cacaGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTcgc                                            >  1:1689023/1‑149 (MQ=255)
                                                                                                          gaaagaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATc                                        <  2:359921/149‑1 (MQ=255)
                                                                                                          gaaagaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATc                                        >  2:2582554/1‑149 (MQ=255)
                                                                                                             agaaaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGc                                     <  1:1030576/149‑1 (MQ=255)
                                                                                                                aaTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTgg                                  >  2:4275447/1‑149 (MQ=255)
                                                                                                                 aTCCATCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGc                                 <  2:162928/149‑1 (MQ=255)
                                                                                                                 aTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGc                                 >  2:4336351/1‑149 (MQ=255)
                                                                                                                 aTCCAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGc                                 <  2:3107377/149‑1 (MQ=255)
                                                                                                                   ccAGCAGTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGctc                               <  2:688260/149‑1 (MQ=255)
                                                                                                                         gTTCAATCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCa                         <  1:1740390/149‑1 (MQ=255)
                                                                                                                             aaTCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGa                     <  1:1413292/149‑1 (MQ=255)
                                                                                                                              aTCAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGaa                    >  2:2067787/1‑149 (MQ=255)
                                                                                                                                cAGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGc                  >  1:3988765/1‑149 (MQ=255)
                                                                                                                                 aGCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGca                 >  1:2175894/1‑149 (MQ=255)
                                                                                                                                  gCTACAGCGCACCATAACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGcac                <  2:4564071/149‑1 (MQ=255)
                                                                                                                                      cAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAAc            >  2:4300459/1‑149 (MQ=255)
                                                                                                                                           caccataaccatTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTAc       <  1:920449/149‑1 (MQ=255)
                                                                                                                                            accataaccatTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACt      >  1:1968730/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GTTTCACATTGCTGGAGGTCATGGCGGCGCTGGCGATTTTCTCAATGCTTAGCGTGCTGGCGTTTATGATTTTTTCGCAAGCTTCCGAGTTGCATCAACGCTCACAGAAAGAAATCCAGCAGTTCAATCAGCTACAGCGCACCATCACCATTCTTGATAACGATCTCCTGCAACTGGTCGCCCGGAGGAATCGGAGTACGGACAAAATCATGGTGTTGGGTGAAGAAGCTATTTTTACCACGCAAAGTCGCGATCCGCTGGCTCCTCTCAGTGAAGCACAAACCTTACTCACT  >  CP009273/3455722‑3456014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑