BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,664,370	G→T	6.7%	A473A (GCG→GCT)	treF →	cytoplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,664,370	0	G	T	6.7%	1194.9 / 44.2	311	A473A (GCG→GCT)	treF	cytoplasmic trehalase
Reads supporting (aligned to +/- strand): ref base G (135/155); new base T (8/13); total (143/168)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

CGGTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCCGCGAAGGCGGCG  >  CP009273/3664223‑3664518

cGGTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACTATGGATGGCGa                                                                                                                                                     <  2:2123022/149‑1 (MQ=255)

  gTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGCTGGCTAtt                                                                                                                                                   >  1:854133/1‑149 (MQ=255)

  gTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGAtt                                                                                                                                                   >  1:3104893/1‑149 (MQ=255)

  gTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGAtt                                                                                                                                                   >  1:160147/1‑149 (MQ=255)

  gTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGAtt                                                                                                                                                   >  2:3099591/1‑149 (MQ=255)

      ggCGAACCATGAACAGGCCGATCGTCTGGCACACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAgg                                                                                                                                               <  2:498892/149‑1 (MQ=255)

       gCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAggg                                                                                                                                              <  1:1975969/149‑1 (MQ=255)

             cATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTaa                                                                                                                                        >  1:4451359/1‑149 (MQ=255)

             cATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCCGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTaa                                                                                                                                        >  1:2109956/1‑149 (MQ=255)

              aTGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTaaaa                                                                                                                                       >  1:4422189/1‑149 (MQ=255)

              aTGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTaaa                                                                                                                                       >  2:3561866/1‑149 (MQ=255)

                gAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAAt                                                                                                                                     >  1:1785355/1‑149 (MQ=255)

                gAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAAt                                                                                                                                     <  1:1131225/149‑1 (MQ=255)

                 aaCAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATg                                                                                                                                    >  2:1726205/1‑149 (MQ=255)

                  aCAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGt                                                                                                                                   <  1:2210435/149‑1 (MQ=255)

                     ggCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACg                                                                                                                                >  1:230946/1‑149 (MQ=255)

                      gCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACgg                                                                                                                               <  2:721799/149‑1 (MQ=255)

                       ccGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGc                                                                                                                              <  2:1652003/149‑1 (MQ=255)

                         gATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGa                                                                                                                            <  2:1681761/149‑1 (MQ=255)

                         gATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGa                                                                                                                            >  2:2562516/1‑149 (MQ=255)

                           tCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCACGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCACCGGCTGGGCACCGTTACAATGGATGGCGAGTCAGGGATTTAAAATGTACGGCGATg                                                                                                                          >  2:3657626/1‑149 (MQ=255)

                           tCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATg                                                                                                                          <  1:3992856/149‑1 (MQ=255)

                           tCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATg                                                                                                                          >  2:1321542/1‑149 (MQ=255)

                              tCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCTATTCAGGGATTTAAAATGTACGGCGATGAcc                                                                                                                       >  1:3874578/1‑149 (MQ=255)

                              tCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGAcc                                                                                                                       >  1:2898214/1‑149 (MQ=255)

                               cTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCt                                                                                                                      >  2:1948738/1‑149 (MQ=255)

                                tGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTCAAATGTACGGCGATGACCtt                                                                                                                     >  2:2472360/1‑149 (MQ=255)

                                  gCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCt                                                                                                                   >  1:1384985/1‑149 (MQ=255)

                                   cAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACACAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTg                                                                                                                  <  1:1491582/149‑1 (MQ=255)

                                     aaCGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTggg                                                                                                                >  1:4682371/1‑149 (MQ=255)

                                         ccGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGtgat                                                                                                            <  1:1724957/149‑1 (MQ=255)

                                          cgggcgcAGGCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGtgatg                                                                                                           <  1:3283283/146‑1 (MQ=255)

                                           gTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCTATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGtgatga                                                                                                          >  1:2950745/1‑149 (MQ=255)

                                             gcgcAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGaaa                                                                                                        <  1:3646991/149‑1 (MQ=255)

                                                 aGTCGGTTACTGACACCTGGGGGGATTCTGGCAAGCGTGTACTAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCTGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATcgc                                                                                                    <  1:2557021/149‑1 (MQ=255)

                                                      gTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGaa                                                                                               <  2:141188/149‑1 (MQ=255)

                                                      gTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGaa                                                                                               >  1:4332046/1‑149 (MQ=255)

                                                        tACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAACTCGCGCGAAgc                                                                                             >  2:4296689/1‑149 (MQ=255)

                                                        tACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAgc                                                                                             <  2:4681801/149‑1 (MQ=255)

                                                         aCTGACACCTGGAGGGATTCTGGCAAGCGAGTACTAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGAACTTCTGGGTGATGAAATCGCGCGAAtct                                                                                            >  2:2080386/1‑149 (MQ=255)

                                                             acacCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCTATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAgctggct                                                                                        <  1:3883655/149‑1 (MQ=255)

                                                             acacCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAgctggct                                                                                        >  2:1860142/1‑149 (MQ=255)

                                                                ccTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGaa                                                                                     >  1:4222195/1‑149 (MQ=255)

                                                                ccTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGaa                                                                                     <  1:4529718/149‑1 (MQ=255)

                                                                  tGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGa                                                                                   >  1:2707150/1‑149 (MQ=255)

                                                                   ggCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGAc                                                                                  <  1:2485620/149‑1 (MQ=255)

                                                                   ggCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGAc                                                                                  <  2:3216490/149‑1 (MQ=255)

                                                                     cGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACgg                                                                                <  2:3439099/149‑1 (MQ=255)

                                                                       ggATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTg                                                                              <  2:4091160/149‑1 (MQ=255)

                                                                       ggATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTg                                                                              >  1:3724916/1‑149 (MQ=255)

                                                                         aTTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGaa                                                                            >  2:4512668/1‑149 (MQ=255)

                                                                           tCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATc                                                                          <  1:1958737/149‑1 (MQ=255)

                                                                               gCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGtt                                                                      <  1:3375325/149‑1 (MQ=255)

                                                                                cAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTc                                                                     <  2:340904/149‑1 (MQ=255)

                                                                                cAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTc                                                                     <  2:2213418/149‑1 (MQ=255)

                                                                                cAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTc                                                                     <  2:1320433/149‑1 (MQ=255)

                                                                                  aGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTa                                                                   >  1:4606542/1‑149 (MQ=255)

                                                                                  aGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTa                                                                   <  1:1533147/149‑1 (MQ=255)

                                                                                   gCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAACTCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTAt                                                                  >  1:4258889/1‑149 (MQ=255)

                                                                                   gCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTAt                                                                  <  1:3667847/149‑1 (MQ=255)

                                                                                   gCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTAt                                                                  >  1:3189434/1‑149 (MQ=255)

                                                                                   gCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTAt                                                                  <  1:3033744/149‑1 (MQ=255)

                                                                                   gCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTAt                                                                  >  1:1881516/1‑149 (MQ=255)

                                                                                   gCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTAt                                                                  <  1:1023186/149‑1 (MQ=255)

                                                                                    cGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATc                                                                 <  2:3897458/149‑1 (MQ=255)

                                                                                             aaCCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGc                                                        >  2:855975/1‑149 (MQ=255)

                                                                                                   tGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAAc                                                  >  1:986749/1‑149 (MQ=255)

                                                                                                    gAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACt                                                 <  1:2056323/149‑1 (MQ=255)

                                                                                                    gAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACt                                                 <  1:4404727/149‑1 (MQ=255)

                                                                                                     aaCAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTg                                                >  2:230700/1‑149 (MQ=255)

                                                                                                      aCAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGa                                               <  1:3916412/149‑1 (MQ=255)

                                                                                                       cAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGAt                                              <  2:772074/149‑1 (MQ=255)

                                                                                                        aGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATc                                             >  1:3826180/1‑149 (MQ=255)

                                                                                                        aGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATc                                             <  1:633812/149‑1 (MQ=255)

                                                                                                         gTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCg                                            >  1:3345233/1‑149 (MQ=255)

                                                                                                         gTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCg                                            >  1:3846043/1‑149 (MQ=255)

                                                                                                           gggATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGaa                                          <  2:2586681/149‑1 (MQ=255)

                                                                                                            ggATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGaaa                                         <  2:2957639/149‑1 (MQ=255)

                                                                                                                  aCCCAACGGCTGGGCACCGTTACATTGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATAcc                                   <  2:3038765/149‑1 (MQ=255)

                                                                                                                  aCCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATAcc                                   <  2:2768994/149‑1 (MQ=255)

                                                                                                                    ccAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCat                                 <  2:1176102/149‑1 (MQ=255)

                                                                                                                     cAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCata                                >  2:1472731/1‑149 (MQ=255)

                                                                                                                      aaCGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCatat                               >  1:516367/1‑149 (MQ=255)

                                                                                                                       aCGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATAtt                              <  2:3868380/149‑1 (MQ=255)

                                                                                                                          gCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGcc                           >  2:4144098/1‑149 (MQ=255)

                                                                                                                           cTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCg                          <  2:4226562/149‑1 (MQ=255)

                                                                                                                             gggCACCGTTACAATGGATGGCGATTCAGTGATATAAAATTAACGGCGATGACCTTCTGGGAGATGAAATCGCGCGAAGCTGCCTGTAGACTGTGAAACAGTTCTATATGGAACAGAACAAAATGATCGAAAAATACCATATTgaagat                        >  1:1217901/1‑144 (MQ=255)

                                                                                                                             gggCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGAt                        >  1:4577645/1‑149 (MQ=255)

                                                                                                                             gggCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGAt                        >  2:1909498/1‑149 (MQ=255)

                                                                                                                             gggCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGAt                        <  1:671011/149‑1 (MQ=255)

                                                                                                                                cACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGgt                     <  1:765045/149‑1 (MQ=255)

                                                                                                                                    gTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTc                 <  1:422510/149‑1 (MQ=255)

                                                                                                                                    gTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTc                 >  1:1101224/1‑149 (MQ=255)

                                                                                                                                     ttACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTcc                >  1:1002830/1‑149 (MQ=255)

                                                                                                                                      tACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTccc               <  2:4073848/149‑1 (MQ=255)

                                                                                                                                          atggatggCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCcgcg           <  2:728253/149‑1 (MQ=255)

                                                                                                                                               tggCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCCGCGAAggc      >  2:1081040/1‑149 (MQ=255)

                                                                                                                                               tggCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCCGCGAAggc      <  2:3063664/149‑1 (MQ=255)

                                                                                                                                                 gCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCCGCGAAggcgg    >  2:3360960/1‑149 (MQ=255)

                                                                                                                                                   gATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCCGCGAAGgcggcg  >  2:632552/1‑149 (MQ=255)

CGGTATGGCGAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGTCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGTACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCGATTCAGGGATTTAAAATGTACGGCGATGACCTTCTGGGTGATGAAATCGCGCGAAGCTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACTGATCGAAAAATACCATATTGCCGATGGTGTTCCCCGCGAAGGCGGCG  >  CP009273/3664223‑3664518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`