BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,748,366	C→A	10.6%	V502V (GTG→GTT)	aldB ←	aldehyde dehydrogenase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,748,366	0	C	A	10.6%	1097.7 / 74.0	304	V502V (GTG→GTT)	aldB	aldehyde dehydrogenase B
Reads supporting (aligned to +/- strand): ref base C (116/155); new base A (17/16); total (133/171)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 307 total aligned reads displayed.

ATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCC  >  CP009273/3748218‑3748512

aTGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTc                                                                                                                                                    >  1:274787/1‑149 (MQ=255)

    cGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACca                                                                                                                                                <  2:29982/149‑1 (MQ=255)

    cGGATGCGGAGTGGACGCCTGATCCGGCCTACGCTCAGGTTCCCGTAGGCATGAGAAGACGCGTAAGCGTCGCATCAGGCAATGAAGACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACca                                                                                                                                                <  1:3612012/149‑1 (MQ=255)

       aTGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACcagca                                                                                                                                             <  1:2028979/149‑1 (MQ=255)

       aTGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTAACcagca                                                                                                                                             <  1:4488692/149‑1 (MQ=255)

                gtACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTgg                                                                                                                                    <  2:4399560/149‑1 (MQ=255)

                  aCGCATTATCCGTCCTACGTTCTGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGtt                                                                                                                                  <  1:593612/149‑1 (MQ=255)

                    gCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTg                                                                                                                                <  2:2651216/149‑1 (MQ=255)

                          tCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTa                                                                                                                          >  1:2335037/1‑149 (MQ=255)

                          tCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTAACCAGCAGGCACTTGGTTTGCTGGTa                                                                                                                          <  1:731884/149‑1 (MQ=255)

                            cGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAAt                                                                                                                        <  1:2409751/149‑1 (MQ=255)

                            cGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAAt                                                                                                                        <  2:3942139/149‑1 (MQ=255)

                            cGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAAt                                                                                                                        >  1:3125070/1‑149 (MQ=255)

                              gCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTAACCAGCAGGCACTTGGTTTGCTGGTAATGc                                                                                                                      <  2:3748581/149‑1 (MQ=255)

                              gCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTAACCAGCAGGCACTTGGTTTGCTGGTAATGc                                                                                                                      <  2:2556057/149‑1 (MQ=255)

                               ccTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTAACCAGCAGGCACTTGGTTTGCTGGTAATGCt                                                                                                                     >  1:3212375/1‑149 (MQ=255)

                                 tACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTcc                                                                                                                   <  1:2815106/149‑1 (MQ=255)

                                  aCGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCa                                                                                                                  <  2:757604/149‑1 (MQ=255)

                                  aCGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTGCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCa                                                                                                                  >  1:805054/1‑149 (MQ=255)

                                   cGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAg                                                                                                                 <  1:4484570/149‑1 (MQ=255)

                                     gTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGca                                                                                                               >  2:2777253/1‑149 (MQ=255)

                                     gTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGca                                                                                                               >  1:958638/1‑149 (MQ=255)

                                     gTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGca                                                                                                               >  1:4135372/1‑149 (MQ=255)

                                      tCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGcat                                                                                                              >  1:2464195/1‑149 (MQ=255)

                                       cAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGcatc                                                                                                             <  2:894071/149‑1 (MQ=255)

                                        aGGTTCCCGTATGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGcatca                                                                                                            <  2:2614808/149‑1 (MQ=255)

                                                  aGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGt                                                                                                  <  1:3320302/149‑1 (MQ=255)

                                                  aGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGt                                                                                                  >  2:2144621/1‑149 (MQ=255)

                                                  aGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGt                                                                                                  <  1:1444188/149‑1 (MQ=255)

                                                    gCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGttt                                                                                                >  2:1506525/1‑149 (MQ=255)

                                                     cATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTc                                                                                               <  1:792904/149‑1 (MQ=255)

                                                      aTGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTAACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTcg                                                                                              >  2:602252/1‑149 (MQ=255)

                                                        gATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTAACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTcgcg                                                                                            >  2:3230105/1‑149 (MQ=255)

                                                         aTAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGa                                                                                           >  1:3963067/1‑149 (MQ=255)

                                                          tAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGAc                                                                                          <  2:2723978/149‑1 (MQ=255)

                                                          tAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGAc                                                                                          <  2:1235830/149‑1 (MQ=255)

                                                          tAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGAc                                                                                          >  1:3391022/1‑149 (MQ=255)

                                                             gACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCGTCTTATCTCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGa                                                                                       <  2:2474502/149‑1 (MQ=255)

                                                               cgcgTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATa                                                                                     <  1:1568157/149‑1 (MQ=255)

                                                                    aaGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGa                                                                                <  2:721555/149‑1 (MQ=255)

                                                                     aGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGAt                                                                               <  1:2124627/149‑1 (MQ=255)

                                                                     aGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGAt                                                                               <  2:4662018/149‑1 (MQ=255)

                                                                      gCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGAtt                                                                              <  2:3174028/149‑1 (MQ=255)

                                                                        gTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGAttgt                                                                            <  2:3048521/149‑1 (MQ=255)

                                                                        gTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGAttgt                                                                            <  1:1088143/149‑1 (MQ=255)

                                                                         tCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGAttgtt                                                                           <  2:2058719/149‑1 (MQ=255)

                                                                         tCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGAttgtt                                                                           <  2:1335480/149‑1 (MQ=255)

                                                                                 ggCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGcca                                                                   >  2:1956995/1‑149 (MQ=255)

                                                                                   cAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGccacc                                                                 <  2:581981/149‑1 (MQ=255)

                                                                                    aaTGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTAACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGccacca                                                                >  1:4276391/1‑149 (MQ=255)

                                                                                     aTGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCaa                                                               <  1:3875967/149‑1 (MQ=255)

                                                                                       gAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAAc                                                             >  1:3381977/1‑149 (MQ=255)

                                                                                        aaTACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTAACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAAcg                                                            >  2:512007/1‑149 (MQ=255)

                                                                                            cccAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAAcgccgc                                                        <  1:1593016/149‑1 (MQ=255)

                                                                                            cccAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAAcgccgc                                                        >  1:1825882/1‑149 (MQ=255)

                                                                                            cccAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAAcgccgc                                                        <  1:1972170/149‑1 (MQ=255)

                                                                                               aaTGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCAtg                                                     >  2:1005705/1‑149 (MQ=255)

                                                                                               aaTGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCAtg                                                     <  2:653859/149‑1 (MQ=255)

                                                                                                aTGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCAtgt                                                    >  1:3973028/1‑149 (MQ=255)

                                                                                                aTGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCAtgt                                                    <  2:4093763/149‑1 (MQ=255)

                                                                                                  gCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGc                                                  >  1:1277681/1‑149 (MQ=255)

                                                                                                   cGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGcc                                                 <  1:291469/149‑1 (MQ=255)

                                                                                                   cGACCAGCTTCTTAGATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGcc                                                 <  2:4533620/149‑1 (MQ=255)

                                                                                                    gACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCg                                                >  1:4414322/1‑149 (MQ=255)

                                                                                                    gACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCg                                                >  1:4217167/1‑149 (MQ=255)

                                                                                                    gACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCg                                                >  1:2821731/1‑149 (MQ=255)

                                                                                                    gACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCg                                                >  2:1750788/1‑149 (MQ=255)

                                                                                                    gACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCg                                                >  2:120004/1‑149 (MQ=255)

                                                                                                      ccAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCggg                                              >  2:2861768/1‑149 (MQ=255)

                                                                                                      ccAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCggg                                              <  2:3700630/149‑1 (MQ=255)

                                                                                                      ccAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCggg                                              >  2:2366586/1‑149 (MQ=255)

                                                                                                       cAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGt                                             >  1:4009969/1‑149 (MQ=255)

                                                                                                       cAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGt                                             >  1:4506849/1‑149 (MQ=255)

                                                                                                           ttcttATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTAACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGc                                         >  2:4056439/1‑149 (MQ=255)

                                                                                                             cttATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGt                                       <  2:4135372/149‑1 (MQ=255)

                                                                                                             cttATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGt                                       >  2:3737180/1‑149 (MQ=255)

                                                                                                              ttATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTg                                      <  1:3547226/149‑1 (MQ=255)

                                                                                                               tataTCCGAACAGCCCCAACGTTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGTTTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGa                                     <  1:297891/149‑1 (MQ=255)

                                                                                                                atatCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGAt                                    >  1:102191/1‑149 (MQ=255)

                                                                                                                  atCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATaa                                  >  2:166511/1‑149 (MQ=255)

                                                                                                                  atCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATaa                                  >  1:3016469/1‑149 (MQ=255)

                                                                                                                    cAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACa                                <  2:1987917/149‑1 (MQ=255)

                                                                                                                    cAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGGGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACa                                <  2:1633068/149‑1 (MQ=255)

                                                                                                                       aaCAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGtt                             >  2:1915818/1‑149 (MQ=255)

                                                                                                                       aaCAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGtt                             <  2:1549604/149‑1 (MQ=255)

                                                                                                                        aCAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTg                            >  2:2792503/1‑149 (MQ=255)

                                                                                                                        aCAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTg                            <  2:4318597/149‑1 (MQ=255)

                                                                                                                        aCAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTg                            >  2:3264659/1‑149 (MQ=255)

                                                                                                                          aGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGt                          <  2:103874/149‑1 (MQ=255)

                                                                                                                            ccccAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTcc                        <  1:1174338/149‑1 (MQ=255)

                                                                                                                                aaCGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCacac                    >  2:2217185/1‑149 (MQ=255)

                                                                                                                                aaCGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCacac                    >  2:364888/1‑149 (MQ=255)

                                                                                                                                  cGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACAcgc                  <  2:3902707/149‑1 (MQ=255)

                                                                                                                                    gTTTATCCGAGTAGCTAACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACgcgc                >  2:3485518/1‑149 (MQ=255)

                                                                                                                                     tttATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGcc               <  1:1048984/149‑1 (MQ=255)

                                                                                                                                      ttATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGccc              >  2:2049737/1‑149 (MQ=255)

                                                                                                                                               tAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTAt     >  1:3695893/1‑149 (MQ=255)

                                                                                                                                               tAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTAt     <  2:3801528/149‑1 (MQ=255)

                                                                                                                                                 gCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGc   <  1:525826/149‑1 (MQ=255)

                                                                                                                                                  cTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGcc  >  2:2291966/1‑149 (MQ=255)

ATGCCGGATGCGGCGTGTACGCCTTATCCGGCCTACGGTCAGGTTCCCGTAGGCATGATAAGACGCGTAAGCGTCGCATCAGGCAATGAATACCCAATGCGACCAGCTTCTTATATCAGAACAGCCCCAACGGTTTATCCGAGTAGCTCACCAGCAGGCACTTGGTTTGCTGGTAATGCTCCAGCATCATCTTGTGGGTTTCGCGACCGATACCTGATTGTTTGTAGCCACCAAACGCCGCATGTGCCGGGTAAGCGTGATAACAGTTGGTCCACACGCGCCCAGCCTGTATGCC  >  CP009273/3748218‑3748512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`