BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,860,159	C→A	88.0%	P33P (CCG→CCT)	ibpB ←	heat shock chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,860,159	0	C	A	88.0%	673.8 / 77.5	268	P33P (CCG→CCT)	ibpB	heat shock chaperone
Reads supporting (aligned to +/- strand): ref base C (11/22); new base A (63/164); total (82/186)
Fisher's exact test for biased strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

TTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATT  >  CP009273/3860013‑3860307

tttttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTAcggagg                                                                                                                                                    <  1:3580893/149‑1 (MQ=255)

tttttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTAcggagg                                                                                                                                                    <  2:1549657/149‑1 (MQ=255)

 ttttCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAggg                                                                                                                                                   <  2:3376625/149‑1 (MQ=255)

     ctctTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGc                                                                                                                                               <  1:3673733/149‑1 (MQ=255)

     ctctTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTATCAAAGTTGTACGGAGGGCAGc                                                                                                                                               >  2:3212760/1‑149 (MQ=255)

      tcttctTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGct                                                                                                                                              <  1:4670013/149‑1 (MQ=255)

      tcttctTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACCGCGGGAAGca                                                                                                                                              >  1:3376631/1‑148 (MQ=255)

       cttcttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGctc                                                                                                                                             <  2:2359592/149‑1 (MQ=255)

         tcttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAAAGTTGTTAGGAGGGGAGCGCTg                                                                                                                                           >  1:1013692/1‑149 (MQ=255)

         tcttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTTCGGCGGGAAGCTCTg                                                                                                                                           >  2:4594824/1‑149 (MQ=255)

         tcttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTg                                                                                                                                           <  2:935463/149‑1 (MQ=255)

         tcttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTGTGAATGTTGTAAGGTGGGGAGCACTg                                                                                                                                           >  1:1976617/1‑149 (MQ=255)

          cttTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTGAATGTTTTAAGGAGGGAAGctcttg                                                                                                                                          >  1:15409/1‑147 (MQ=255)

            tttGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCt                                                                                                                                        <  2:1990994/149‑1 (MQ=255)

            tttGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCt                                                                                                                                        <  2:4325012/149‑1 (MQ=255)

             ttGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTGGAAATGTTTTACGGAGGGAAGCTCAGGGtt                                                                                                                                       >  1:3300923/1‑149 (MQ=255)

              tGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCttt                                                                                                                                      <  1:1507588/149‑1 (MQ=255)

                 ctgctCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCAc                                                                                                                                   <  1:59822/149‑1 (MQ=255)

                  tgctCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGACAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAATGTTGTAAGGAGTGAAGCGGTGGCTTTGAcc                                                                                                                                  >  1:2056672/1‑149 (MQ=255)

                    ctCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCgg                                                                                                                                <  1:81105/149‑1 (MQ=255)

                     tCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGc                                                                                                                               <  2:4151233/149‑1 (MQ=255)

                      ccGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCg                                                                                                                              <  2:4297614/149‑1 (MQ=255)

                      ccGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCGCTGGCTTTCACCGGCg                                                                                                                              >  2:714029/1‑149 (MQ=255)

                       cGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGt                                                                                                                             <  1:2212513/149‑1 (MQ=255)

                        ggCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtt                                                                                                                            <  1:3484969/149‑1 (MQ=255)

                        ggCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtt                                                                                                                            <  2:2322675/149‑1 (MQ=255)

                         gCGTGCCTTTTCCGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGttt                                                                                                                           <  2:3271642/149‑1 (MQ=255)

                          cGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGtttt                                                                                                                          <  1:917998/149‑1 (MQ=255)

                           gTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTAAGGAGGGGAGCTCTTGCGTTCACCGGCGGTTTg                                                                                                                         >  2:22664/1‑149 (MQ=255)

                            tGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGc                                                                                                                        <  2:4190201/149‑1 (MQ=255)

                               cTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGt                                                                                                                     >  1:3889959/1‑149 (MQ=255)

                                ttttACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTAACCGGCGTTTTGCAGTg                                                                                                                    >  2:1262804/1‑149 (MQ=255)

                                  ttACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATTTTGTACGGAGGGAAGCGCTGGCGTTCACCGGCGTTTTGCAGTGCg                                                                                                                  >  2:533959/1‑149 (MQ=255)

                                   tACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGt                                                                                                                 <  2:3547219/149‑1 (MQ=255)

                                       cTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGc                                                                                                             <  2:4081686/149‑1 (MQ=255)

                                         cAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCa                                                                                                           <  1:2299732/149‑1 (MQ=255)

                                          aGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCGTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGGGTTGCAGTTCGTTGGCCAg                                                                                                          >  2:3667171/1‑149 (MQ=255)

                                                gTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTc                                                                                                    <  1:3808352/149‑1 (MQ=255)

                                                        cAGGTGAATCTCTAAATCGTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGa                                                                                            <  2:2944226/149‑1 (MQ=255)

                                                         aGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGAt                                                                                           <  2:547530/149‑1 (MQ=255)

                                                          gTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATc                                                                                          <  2:3404653/149‑1 (MQ=255)

                                                          gTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATc                                                                                          <  2:4516446/149‑1 (MQ=255)

                                                          gTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATc                                                                                          <  2:3583755/149‑1 (MQ=255)

                                                           ttGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTTTGAAAGTTGTTAGGAGGGCAGCTCTGGCTTTAAACGGCGGTTTTGAGTGGGTTTGGCACTTTTTTaaaaaacaaac                                                                                         >  1:807237/1‑142 (MQ=255)

                                                           ttGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATcc                                                                                         <  1:3234441/149‑1 (MQ=255)

                                                             gAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCAt                                                                                       <  1:2608398/149‑1 (MQ=255)

                                                              aaTCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCAtt                                                                                      <  2:2635097/149‑1 (MQ=255)

                                                              aaTCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCAtt                                                                                      <  2:1150660/149‑1 (MQ=255)

                                                                tctcTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGa                                                                                    <  1:509410/149‑1 (MQ=255)

                                                                tctcTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGa                                                                                    <  2:1572843/149‑1 (MQ=255)

                                                                tctcTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGa                                                                                    <  2:1235395/149‑1 (MQ=255)

                                                                 ctctAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGAc                                                                                   <  1:705502/149‑1 (MQ=255)

                                                                 ctctAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGAc                                                                                   <  1:3081437/149‑1 (MQ=255)

                                                                   ctAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGc                                                                                 <  1:2664546/149‑1 (MQ=255)

                                                                    tAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCa                                                                                <  2:3758781/149‑1 (MQ=255)

                                                                     aaaTCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTGAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAAACGATCCCTTGACGCAt                                                                               >  2:1902831/1‑149 (MQ=255)

                                                                      aaTCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTGAATGTTTTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGAAGTGGGTTGGCCAGGTTGTCAAAAACGATCCATTGACGCAta                                                                              >  2:887312/1‑148 (MQ=255)

                                                                          ttCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGGTTTGCAGTGCGTTGGCCCGGTTGTCAAAACCGATCCAATGACGCCTccggg                                                                          >  2:1751232/1‑145 (MQ=255)

                                                                           tCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTgg                                                                         <  1:1315907/149‑1 (MQ=255)

                                                                               gACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGAt                                                                     <  1:4268000/149‑1 (MQ=255)

                                                                                       ccTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGaa                                                             <  2:346118/149‑1 (MQ=255)

                                                                                        cTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCCTCAGTGGGGATAAATCGAAg                                                            >  2:1041840/1‑149 (MQ=255)

                                                                                         tGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTGTCAAAGTTTTACGGAGGGAAGCACTGGCGTTCACCGGCGGTTTGCAGTGCGTTGGCCCGGTTTTCAAAACCCATCCCATGACCCATCAGTGGGGGTAAATCGAAGt                                                           >  1:1473329/1‑149 (MQ=255)

                                                                                          gCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGtt                                                          <  1:406027/149‑1 (MQ=255)

                                                                                              gcgcAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGc                                                      <  1:2913796/149‑1 (MQ=255)

                                                                                                gcAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGCCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCAt                                                    <  1:1740047/149‑1 (MQ=255)

                                                                                                 cAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATa                                                   <  1:2947134/149‑1 (MQ=255)

                                                                                                 cAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATa                                                   <  2:1922212/149‑1 (MQ=255)

                                                                                                  aaGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAg                                                  <  1:1799987/149‑1 (MQ=255)

                                                                                                  aaGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAg                                                  <  2:504376/149‑1 (MQ=255)

                                                                                                   aGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGt                                                 <  2:1692877/149‑1 (MQ=255)

                                                                                                    gggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGGAGCTCTGGCTTTAAACGGCGTTTTGCATTTCGTTGGCCAGTTTGTCAAAAACGATCCCTTTACGCATAAGTGGGGATAAATAGAAGTTAAGCATAGTc                                                >  2:1492810/1‑149 (MQ=255)

                                                                                                     ggTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCa                                               <  1:566814/149‑1 (MQ=255)

                                                                                                         aTGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTtc                                           <  2:2437600/149‑1 (MQ=255)

                                                                                                             ggTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCt                                       <  2:184496/149‑1 (MQ=255)

                                                                                                             ggTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCt                                       <  1:2155192/149‑1 (MQ=255)

                                                                                                             ggTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCt                                       <  2:3045137/149‑1 (MQ=255)

                                                                                                              gTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCtt                                      <  1:1832941/149‑1 (MQ=255)

                                                                                                              gTAGTGGTTATCGTCGCTTTTATAAAAGTTTTACGGAGGGAACCTCTGGCTTTCAACCGCCTTTTGCAGTGCGGTGGCCACGTTGTCAAAAACCATCAATTTACGCCACAGGGGGGCTAAATAGAAATTAAGCAAATTCAATTAACCtt                                      >  2:1676310/1‑149 (MQ=255)

                                                                                                               tAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTc                                     <  1:139931/149‑1 (MQ=255)

                                                                                                               tAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTc                                     <  1:139796/149‑1 (MQ=255)

                                                                                                                aGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCt                                    <  2:574674/149‑1 (MQ=255)

                                                                                                                 gTGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTa                                   <  2:2169619/149‑1 (MQ=255)

                                                                                                                  tGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTaa                                  <  1:1334019/149‑1 (MQ=255)

                                                                                                                  tGGTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTaa                                  <  1:2737502/149‑1 (MQ=255)

                                                                                                                   ggTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTaag                                 <  2:4419097/149‑1 (MQ=255)

                                                                                                                   ggTTATCGTCGCTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTaag                                 <  2:4403442/149‑1 (MQ=255)

                                                                                                                              cTTTTCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaag                      <  1:1023306/149‑1 (MQ=255)

                                                                                                                               ttttCTCAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGagtaagt                     <  1:4166269/149‑1 (MQ=255)

                                                                                                                                  tctcACTGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTAcc                  <  1:127354/149‑1 (MQ=255)

                                                                                                                                  tctcAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTAcc                  <  2:414304/149‑1 (MQ=255)

                                                                                                                                  tctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTAcc                  <  1:2469848/149‑1 (MQ=255)

                                                                                                                                  tctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTAcc                  <  1:1154240/149‑1 (MQ=255)

                                                                                                                                  tctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTCTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTAcc                  <  2:690991/149‑1 (MQ=255)

                                                                                                                                   ctcAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCt                 <  1:1276154/149‑1 (MQ=255)

                                                                                                                                   ctcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCt                 <  2:3889959/149‑1 (MQ=255)

                                                                                                                                    tcAATGTTGTACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTg                <  1:157647/149‑1 (MQ=255)

                                                                                                                                         gttgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAAt           <  2:136154/149‑1 (MQ=255)

                                                                                                                                         gttgtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAAt           <  2:2718562/149‑1 (MQ=255)

                                                                                                                                            gtACGGAGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCg        >  2:2314546/1‑149 (MQ=255)

TTTTTCTCTTCTTTTGGCTGCTCCGGCGTGCCTTTTACGCTCAGGCGCGTACCTTCCAGTTGAATCTCTAAATCTTCCTGACGGAAACCTGCCAGCGCAAGGGTAATGCGGTAGTGGTTATCGTCGCTTTTCTCAATGTTGTACGGCGGGAAGCTCTGGCTTTCACCGGCGTTTTGCAGTGCGTTGGCCAGTTTGTCAAAACCGATCCATTGACGCATCAGTGGGGATAAATCGAAGTTACGCATAGTCATTTCTCCTTCTAAGAAGCGAGTAAGTACCTGCAAATCCGAAGATT  >  CP009273/3860013‑3860307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`