BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,869,280	G→T	5.6%	M161I (ATG→ATT)	yidX →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,869,280	0	G	T	5.6%	961.4 / 26.4	250	M161I (ATG→ATT)	yidX	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base G (135/101); new base T (9/5); total (144/106)
Fisher's exact test for biased strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 254 total aligned reads displayed.

TTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTT  >  CP009273/3869132‑3869427

ttACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACACTCCATGAGAAAAATAAAAACATg                                                                                                                                                     >  1:1618183/1‑149 (MQ=255)

ttACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATg                                                                                                                                                     <  2:474227/149‑1 (MQ=255)

   ccGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGAt                                                                                                                                                  <  2:438156/149‑1 (MQ=255)

     ggCACTATATCGCTTTACTACTTCTGGAATAACGAAGTAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTa                                                                                                                                                <  1:3932339/149‑1 (MQ=255)

     ggCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTa                                                                                                                                                <  1:323536/149‑1 (MQ=255)

      gCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTAc                                                                                                                                               >  2:2394906/1‑149 (MQ=255)

                 cTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATTGATTACTCAAAGGTGAt                                                                                                                                    <  2:4188400/149‑1 (MQ=255)

                      ctactactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATTGATTACTCAAAGGTGATGGCGt                                                                                                                               >  1:1801507/1‑149 (MQ=255)

                       tactactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGtt                                                                                                                              >  2:4583476/1‑149 (MQ=255)

                       tactactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGtt                                                                                                                              >  2:2242257/1‑149 (MQ=255)

                        ccgactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTc                                                                                                                             <  2:701506/146‑1 (MQ=255)

                        actactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTc                                                                                                                             <  1:320296/149‑1 (MQ=255)

                         ctactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCt                                                                                                                            <  2:3179031/149‑1 (MQ=255)

                          tactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTa                                                                                                                           >  1:4382783/1‑149 (MQ=255)

                          tactGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTa                                                                                                                           <  1:2670078/149‑1 (MQ=255)

                           actGGAATAACGAAGAATAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAc                                                                                                                          <  1:1627215/149‑1 (MQ=255)

                            ctGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAcc                                                                                                                         >  2:2497217/1‑149 (MQ=255)

                            ctGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAcc                                                                                                                         >  1:317153/1‑149 (MQ=255)

                            ctGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAcc                                                                                                                         >  1:317128/1‑149 (MQ=255)

                                aaTAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTAccatcc                                                                                                                     <  2:2749802/149‑1 (MQ=255)

                                   aaCGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCAtt                                                                                                                  >  1:200624/1‑149 (MQ=255)

                                   aaCGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCAtt                                                                                                                  <  2:3881195/149‑1 (MQ=255)

                                    aCGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCAttt                                                                                                                 >  1:3916395/1‑149 (MQ=255)

                                     cGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTa                                                                                                                <  1:74831/149‑1 (MQ=255)

                                       aagaagaaAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTaaa                                                                                                              >  1:1096797/1‑149 (MQ=255)

                                        agaagaaAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAg                                                                                                             >  1:4599695/1‑149 (MQ=255)

                                        agaagaaAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAg                                                                                                             >  2:895387/1‑149 (MQ=255)

                                         gaagaaAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGt                                                                                                            <  1:2645012/149‑1 (MQ=255)

                                          aagaaAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTg                                                                                                           >  1:4201864/1‑149 (MQ=255)

                                           agaaAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGc                                                                                                          <  1:1897671/149‑1 (MQ=255)

                                             aaaaGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGa                                                                                                        <  2:53691/149‑1 (MQ=255)

                                              aaaGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGAt                                                                                                       >  1:4552845/1‑149 (MQ=255)

                                               aaGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGAtt                                                                                                      <  2:2788509/149‑1 (MQ=255)

                                                aGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGAttt                                                                                                     <  1:4483055/149‑1 (MQ=255)

                                                 ggCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGAtttt                                                                                                    >  1:2406743/1‑149 (MQ=255)

                                                 ggCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGAtttt                                                                                                    >  1:211083/1‑149 (MQ=255)

                                                   cACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTAt                                                                                                  <  1:2788768/149‑1 (MQ=255)

                                                     cTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGa                                                                                                >  2:3135070/1‑149 (MQ=255)

                                                       ggCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAAt                                                                                              <  1:3485879/149‑1 (MQ=255)

                                                       ggCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAAt                                                                                              <  2:3984865/149‑1 (MQ=255)

                                                           aCGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTa                                                                                          >  2:377151/1‑149 (MQ=255)

                                                              cATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTc                                                                                       >  1:5604/1‑149 (MQ=255)

                                                               aTTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCa                                                                                      >  1:3092400/1‑149 (MQ=255)

                                                                ttATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCAc                                                                                     >  2:2834806/1‑149 (MQ=255)

                                                                ttATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCAc                                                                                     >  2:4242815/1‑149 (MQ=255)

                                                                     gTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCaga                                                                                >  1:1347910/1‑149 (MQ=255)

                                                                      ttttGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCagag                                                                               <  2:3355102/149‑1 (MQ=255)

                                                                      ttttGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCagag                                                                               <  1:991017/149‑1 (MQ=255)

                                                                             tgtgGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGACTACTATTCACCCAGAGGCATTcc                                                                        >  1:4256420/1‑149 (MQ=255)

                                                                             tgtgGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTcc                                                                        >  2:3710719/1‑149 (MQ=255)

                                                                              gtggtgTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCg                                                                       >  1:2226123/1‑149 (MQ=255)

                                                                                ggtgTTCAACACTGTACCAGGTCACGGGAAAACCTACAAGGCACAATCCATGAGAAAAAGAAAAACATGGATTACTCACAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTAGTCACCCAGAGGCATTCCGGa                                                                     <  2:2677/149‑1 (MQ=255)

                                                                                   gtTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATgg                                                                  <  1:365337/149‑1 (MQ=255)

                                                                                      cAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGgtgt                                                               <  2:4062015/149‑1 (MQ=255)

                                                                                         cacTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATTGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTc                                                            <  2:3307635/149‑1 (MQ=255)

                                                                                         cacTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTc                                                            <  1:3546420/149‑1 (MQ=255)

                                                                                               aCCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCgcagc                                                      >  2:1499210/1‑149 (MQ=255)

                                                                                                ccAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCgcagca                                                     >  1:2372750/1‑149 (MQ=255)

                                                                                                ccAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCgcagca                                                     >  1:121129/1‑149 (MQ=255)

                                                                                                  aGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCAtt                                                   <  1:4314346/149‑1 (MQ=255)

                                                                                                   ggTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTa                                                  >  2:1782504/1‑149 (MQ=255)

                                                                                                   ggTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTa                                                  >  1:175991/1‑149 (MQ=255)

                                                                                                    gTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTAc                                                 <  1:1317294/149‑1 (MQ=255)

                                                                                                     tCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACt                                                >  2:2107977/1‑149 (MQ=255)

                                                                                                     tCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACt                                                <  1:207166/149‑1 (MQ=255)

                                                                                                      cACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTg                                               >  1:157293/1‑149 (MQ=255)

                                                                                                      cACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTg                                               >  2:444688/1‑149 (MQ=255)

                                                                                                        cTGGAAACCCGAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGcc                                             <  1:3980310/149‑1 (MQ=255)

                                                                                                        cTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGcc                                             >  2:1151530/1‑149 (MQ=255)

                                                                                                            aaaaCCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTg                                         >  2:3883378/1‑149 (MQ=255)

                                                                                                              aaCCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGAc                                       >  1:1497100/1‑149 (MQ=255)

                                                                                                               aCCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACt                                      >  2:281624/1‑149 (MQ=255)

                                                                                                               aCCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACt                                      <  1:1001061/149‑1 (MQ=255)

                                                                                                               aCCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACt                                      >  2:4359409/1‑149 (MQ=255)

                                                                                                                 cTAAAAGGCACAATCCATGAGAAAAATAAAAACATTGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGt                                    <  2:1801507/149‑1 (MQ=255)

                                                                                                                  tAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGtt                                   >  2:3535158/1‑149 (MQ=255)

                                                                                                                   aaaaGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTa                                  >  1:4095481/1‑149 (MQ=255)

                                                                                                                   aaaaGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTa                                  <  1:273972/149‑1 (MQ=255)

                                                                                                                   aaaaGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTa                                  >  1:4095904/1‑149 (MQ=255)

                                                                                                                      aGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGTGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGc                               >  1:1279580/1‑149 (MQ=255)

                                                                                                                      aGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGc                               <  1:3300125/149‑1 (MQ=255)

                                                                                                                      aGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGc                               <  2:4408431/149‑1 (MQ=255)

                                                                                                                            aaTCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAca                         <  1:2608596/149‑1 (MQ=255)

                                                                                                                            aaTCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAca                         <  1:1652548/149‑1 (MQ=255)

                                                                                                                              tCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAcatc                       >  2:3085519/1‑149 (MQ=255)

                                                                                                                              tCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAcatc                       >  2:114604/1‑149 (MQ=255)

                                                                                                                                cATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGAcatcat                     >  2:3457754/1‑149 (MQ=255)

                                                                                                                                  tGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTa                   >  1:2541760/1‑149 (MQ=255)

                                                                                                                                   gagaAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTAc                  >  1:3749512/1‑149 (MQ=255)

                                                                                                                                   gagaAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTAc                  <  1:957637/149‑1 (MQ=255)

                                                                                                                                     gaAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTg                <  2:200624/149‑1 (MQ=255)

                                                                                                                                     gaAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTg                >  2:1402563/1‑149 (MQ=255)

                                                                                                                                      aaaaaTAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGc               <  2:943309/149‑1 (MQ=255)

                                                                                                                                      aaaaaTAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCCGGACATCATTACTGc               >  1:3349985/1‑149 (MQ=255)

                                                                                                                                          aTAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCg           >  2:1613117/1‑149 (MQ=255)

                                                                                                                                             aaaaCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTg        >  2:3006661/1‑149 (MQ=255)

                                                                                                                                                aCATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCaa     >  2:158585/1‑149 (MQ=255)

                                                                                                                                                 cATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAAt    >  1:3013505/1‑149 (MQ=255)

                                                                                                                                                  aTGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTAAGCTGGACATCATTACTGCACCGCTGCAAtt   >  2:4573673/1‑149 (MQ=255)

                                                                                                                                                   tGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAAttt  >  2:3943407/1‑149 (MQ=255)

TTACCGGCACAATATCGCTTTACTACTACTGGAATAACGAAGAAGAAAAGGCACTGGCAACGCATTATGGTTTTGCCTGTGGTGTTCAACACTGTACCAGGTCACTGGAAAACCTAAAAGGCACAATCCATGAGAAAAATAAAAACATGGATTACTCAAAGGTGATGGCGTTCTACCATCCATTTAAAGTGCGATTTTATGAATACTATTCACCCAGAGGCATTCCGGATGGTGTTTCCGCAGCATTACTGCCAGTGACTGTTACGCTGGACATCATTACTGCACCGCTGCAATTT  >  CP009273/3869132‑3869427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`