Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,056,018 T→A 90.6% F138I (TTC→ATC)  yihN → MFS transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,056,0180TA90.6% 1152.5 / 88.7 328F138I (TTC→ATC) yihNMFS transporter family protein
Reads supporting (aligned to +/- strand):  ref base T (18/13);  new base A (118/179);  total (136/192)
Fisher's exact test for biased strand distribution p-value = 5.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 332 total aligned reads displayed.

ATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTT  >  CP009273/4055872‑4056163
                                                                                                                                                  |                                                                                                                                                 
aTTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCttcatc                                                                                                                                                 >  1:316083/1‑149 (MQ=255)
 tttGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCg                                                                                                                                                >  1:838423/1‑149 (MQ=255)
    gacgacAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGaaa                                                                                                                                             <  2:1516097/149‑1 (MQ=255)
    gacgacAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGaaa                                                                                                                                             >  2:3267324/1‑149 (MQ=255)
     acgacAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAAc                                                                                                                                            <  2:1891057/149‑1 (MQ=255)
     acgacAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAAc                                                                                                                                            >  2:2165409/1‑149 (MQ=255)
     acgacAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAAc                                                                                                                                            >  1:4105918/1‑149 (MQ=255)
      cgacAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACa                                                                                                                                           >  1:1784902/1‑149 (MQ=255)
        acAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAgg                                                                                                                                         >  1:2541278/1‑149 (MQ=255)
        acAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAgg                                                                                                                                         >  1:3995522/1‑149 (MQ=255)
        acAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAgg                                                                                                                                         >  1:1339078/1‑149 (MQ=255)
          aaTGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATTATGAACTGGCCGGTCTTTCTCAAGTCGGTAATTAGATTGGGCAAAAGCGAAAAACAATGTAGGTTGTTTGTCTTAATCGAAAAAGGGc                                                                                                                                       >  1:395150/1‑149 (MQ=255)
                 gggTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCAt                                                                                                                                <  1:4178414/149‑1 (MQ=255)
                   gTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTg                                                                                                                              >  1:1246644/1‑149 (MQ=255)
                   gTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTg                                                                                                                              <  2:1933341/149‑1 (MQ=255)
                        ggggCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGAt                                                                                                                         >  1:3365322/1‑149 (MQ=255)
                        ggggCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGAt                                                                                                                         >  1:1112642/1‑149 (MQ=255)
                          ggCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATAc                                                                                                                       >  1:3249516/1‑149 (MQ=255)
                              tCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCgtg                                                                                                                   <  1:3232387/149‑1 (MQ=255)
                               ccTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCgtgg                                                                                                                  >  1:1019503/1‑149 (MQ=255)
                               ccTCTTTGTCTGGGCGCTATTTGGAGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCgtgg                                                                                                                  >  1:639363/1‑149 (MQ=255)
                                ctctTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCgtggt                                                                                                                 >  2:2535151/1‑149 (MQ=255)
                                   tttGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGc                                                                                                              >  2:3429495/1‑149 (MQ=255)
                                     tGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCAt                                                                                                            >  2:4245302/1‑149 (MQ=255)
                                         tGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTc                                                                                                        <  1:2629566/149‑1 (MQ=255)
                                         tGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTc                                                                                                        >  1:296191/1‑149 (MQ=255)
                                            gcgcTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGc                                                                                                     <  1:4574351/149‑1 (MQ=255)
                                             cgcTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCg                                                                                                    <  2:858869/149‑1 (MQ=255)
                                              gcTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGt                                                                                                   >  1:2310737/1‑149 (MQ=255)
                                              gcTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGt                                                                                                   <  1:2028525/149‑1 (MQ=255)
                                                tATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTg                                                                                                 >  2:1609735/1‑149 (MQ=255)
                                                tATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTg                                                                                                 <  2:3563219/149‑1 (MQ=255)
                                                 aTTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTgg                                                                                                <  2:2386142/149‑1 (MQ=255)
                                                      gCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTa                                                                                           <  2:4038173/149‑1 (MQ=255)
                                                         ttACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAttt                                                                                        <  2:1211996/149‑1 (MQ=255)
                                                         ttACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTAttt                                                                                        >  1:3103010/1‑149 (MQ=255)
                                                          tACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTa                                                                                       <  2:2607892/149‑1 (MQ=255)
                                                            cTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTAcc                                                                                     <  2:4641100/149‑1 (MQ=255)
                                                            cTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTAcc                                                                                     >  2:2445172/1‑149 (MQ=255)
                                                            cTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTAcc                                                                                     <  1:1938986/149‑1 (MQ=255)
                                                             ttGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCt                                                                                    >  1:4361706/1‑149 (MQ=255)
                                                              tGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTg                                                                                   <  2:1745910/149‑1 (MQ=255)
                                                              tGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTg                                                                                   <  2:3876016/149‑1 (MQ=255)
                                                              tGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTg                                                                                   <  2:4356101/149‑1 (MQ=255)
                                                                  aCATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGttt                                                                               >  1:3735610/1‑149 (MQ=255)
                                                                   cATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTg                                                                              <  1:2315332/149‑1 (MQ=255)
                                                                   cATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTg                                                                              <  2:106392/149‑1 (MQ=255)
                                                                    atgatgAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTgg                                                                             <  2:3365322/149‑1 (MQ=255)
                                                                     tgatgaACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTgtc                                                                            >  2:3040191/1‑147 (MQ=255)
                                                                     tgatgaACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGc                                                                            >  1:2360728/1‑149 (MQ=255)
                                                                     tgatgaACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGc                                                                            >  1:893791/1‑149 (MQ=255)
                                                                     tgatgaACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGc                                                                            <  1:4194712/149‑1 (MQ=255)
                                                                     tgatgaACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGGTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGc                                                                            >  1:4634735/1‑149 (MQ=255)
                                                                      gatgaACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCa                                                                           >  2:3610909/1‑149 (MQ=255)
                                                                           aCTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGc                                                                      <  1:204286/149‑1 (MQ=255)
                                                                              ggCCGGTCGTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCtta                                                                   <  1:1647059/149‑1 (MQ=255)
                                                                               gCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCttat                                                                  <  2:395150/149‑1 (MQ=255)
                                                                                  ggTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAg                                                               <  1:1910458/149‑1 (MQ=255)
                                                                                   gTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAgg                                                              >  1:121445/1‑149 (MQ=255)
                                                                                    tCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGt                                                             <  2:3808881/149‑1 (MQ=255)
                                                                                     cTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGtt                                                            <  1:3647978/149‑1 (MQ=255)
                                                                                     cTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGtt                                                            <  2:811575/149‑1 (MQ=255)
                                                                                         cTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTaa                                                        <  2:3219861/149‑1 (MQ=255)
                                                                                         cTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTaa                                                        <  2:882769/149‑1 (MQ=255)
                                                                                          tCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTaaa                                                       <  2:4087333/149‑1 (MQ=255)
                                                                                          tCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTaaa                                                       >  1:127524/1‑149 (MQ=255)
                                                                                          tCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTaaa                                                       >  1:988041/1‑149 (MQ=255)
                                                                                          tCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTaaa                                                       <  2:4105918/149‑1 (MQ=255)
                                                                                                   tAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATc                                              <  2:3994451/149‑1 (MQ=255)
                                                                                                   tAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATc                                              <  1:3466850/149‑1 (MQ=255)
                                                                                                    aaGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCt                                             <  1:2530401/149‑1 (MQ=255)
                                                                                                     aGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTg                                            <  2:1112642/149‑1 (MQ=255)
                                                                                                       tCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGt                                          >  2:1316589/1‑149 (MQ=255)
                                                                                                       tCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGt                                          <  2:2763440/149‑1 (MQ=255)
                                                                                                       tCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGt                                          <  2:2893104/149‑1 (MQ=255)
                                                                                                       tCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGt                                          <  1:3684216/149‑1 (MQ=255)
                                                                                                         gATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTc                                        <  1:4318318/149‑1 (MQ=255)
                                                                                                          aTTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCt                                       <  2:3182324/149‑1 (MQ=255)
                                                                                                          aTTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCt                                       <  1:4231595/149‑1 (MQ=255)
                                                                                                           ttGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTa                                      >  2:1025388/1‑149 (MQ=255)
                                                                                                           ttGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCGTATTAGGTTTTAAAGCAGGCATCTGGTTCTa                                      >  1:1192950/1‑149 (MQ=255)
                                                                                                           ttGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCGTATTAGGTTTTAAAGCAGGCATCTGGTTCTa                                      >  2:2010526/1‑149 (MQ=255)
                                                                                                             gggCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGAAACCGTGGTGGCATAATCTGCGTTGGCAGTAATTACCAGGTTTGGCAGTGGCTTATTAGGATTAAAAGCAGGCATATGGTTCTAtt                                    >  1:1139710/1‑149 (MQ=255)
                                                                                                              ggCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCATAGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTATGCGTTGGCAGTATTTACCTGGTTTGTCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTc                                   >  1:2115363/1‑149 (MQ=255)
                                                                                                               gCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTcc                                  >  2:4093530/1‑149 (MQ=255)
                                                                                                                  aTAGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCtt                               <  1:4049040/149‑1 (MQ=255)
                                                                                                                    aGCGAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTAt                             <  1:103179/149‑1 (MQ=255)
                                                                                                                       gAACAACAAGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATtgt                          <  1:78935/149‑1 (MQ=255)
                                                                                                                            acaaGGTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTATGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAATCAGGCATCTGGTTATATTGCCTTATTGTGtttt                     >  1:1581184/1‑148 (MQ=255)
                                                                                                                              aaGGGCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTGATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGAGTGcc                   >  2:61444/1‑149 (MQ=255)
                                                                                                                                ggTCGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCCTCTGGTTCTATTCCCTTATTGTGATTGCCGt                 >  2:2313667/1‑149 (MQ=255)
                                                                                                                                   cGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAgg              >  1:277113/1‑149 (MQ=255)
                                                                                                                                   cGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAgg              <  2:673647/149‑1 (MQ=255)
                                                                                                                                   cGGTTGTTTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAgg              <  2:2487061/149‑1 (MQ=255)
                                                                                                                                    ggTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGc             <  1:2870696/149‑1 (MQ=255)
                                                                                                                                        gttTGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCatta         <  2:296191/149‑1 (MQ=255)
                                                                                                                                          ttGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCattatt       <  2:3671842/149‑1 (MQ=255)
                                                                                                                                          ttGGCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCattatt       >  2:430346/1‑149 (MQ=255)
                                                                                                                                             gCTTCATCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCattattatt    <  1:518502/149‑1 (MQ=255)
                                                                                                                                               ttcatcGAAACAGGGCGTGGCATGGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTAtttt  <  2:2646567/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTCGGTTGTTTGGCTTCTTCGAAACAGGGCGTGGCATTGTCGATACCGTGGTGGCATTTTCTGCGTTGGCAGTATTTACCTGGTTTGGCAGTGGCTTATTAGGTTTTAAAGCAGGCATCTGGTTCTATTCCCTTATTGTGATTGCCGTAGGCATTATTATTTT  >  CP009273/4055872‑4056163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑