Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,127,022 T→G 79.6% E188D (GAA→GAC)  yijF ← DUF1287 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,127,0220TG79.6% 709.3 / 139.3 252E188D (GAA→GAC) yijFDUF1287 family protein
Reads supporting (aligned to +/- strand):  ref base T (35/17);  new base G (130/70);  total (165/87)
Fisher's exact test for biased strand distribution p-value = 8.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

TGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTT  >  CP009273/4126874‑4127168
                                                                                                                                                    |                                                                                                                                                  
tgattgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACAtct                                                                                                                                                    <  2:3416582/149‑1 (MQ=255)
   ttgtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACAtcgtct                                                                                                                                                 >  2:3579968/1‑149 (MQ=255)
     gtgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCgtcttc                                                                                                                                               >  1:2865235/1‑149 (MQ=255)
      tgctgGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTcc                                                                                                                                              >  2:609260/1‑149 (MQ=255)
          ggCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCCGTTAAACAGCACATCTTCTTCCTgcg                                                                                                                                          >  1:1574056/1‑149 (MQ=255)
           gCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTgcgc                                                                                                                                         <  2:752829/149‑1 (MQ=255)
              cTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGcc                                                                                                                                      <  2:3948328/149‑1 (MQ=255)
               tGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCt                                                                                                                                     <  2:1314971/149‑1 (MQ=255)
                    cTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGcc                                                                                                                                >  2:2958559/1‑149 (MQ=255)
                      ggTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCaa                                                                                                                              <  1:43611/149‑1 (MQ=255)
                         gAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGt                                                                                                                           >  2:2614567/1‑149 (MQ=255)
                         gAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGt                                                                                                                           >  2:734232/1‑149 (MQ=255)
                             cGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATg                                                                                                                       <  2:2111790/149‑1 (MQ=255)
                              gTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGt                                                                                                                      >  2:2215689/1‑149 (MQ=255)
                               tAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTa                                                                                                                     >  2:1945706/1‑149 (MQ=255)
                                   aaaaaaGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCAc                                                                                                                 <  1:3442333/149‑1 (MQ=255)
                                    aaaaaGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCAcc                                                                                                                >  2:3047949/1‑149 (MQ=255)
                                            gCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGt                                                                                                        >  2:932133/1‑149 (MQ=255)
                                                tCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCg                                                                                                    >  1:3929571/1‑149 (MQ=255)
                                                 cAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGt                                                                                                   <  1:3157669/149‑1 (MQ=255)
                                                  aGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTc                                                                                                  <  1:2051048/149‑1 (MQ=255)
                                                   gtgtAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTcg                                                                                                 >  1:4103189/1‑149 (MQ=255)
                                                    tgtAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTcgc                                                                                                >  2:3204776/1‑149 (MQ=255)
                                                      tAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCgg                                                                                              >  2:1734997/1‑149 (MQ=255)
                                                      tAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCgg                                                                                              <  2:2756937/149‑1 (MQ=255)
                                                       aaaaaGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCggg                                                                                             >  1:1204861/1‑149 (MQ=255)
                                                        aaaaGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGc                                                                                            >  2:383610/1‑149 (MQ=255)
                                                        aaaaGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGc                                                                                            <  2:3446589/149‑1 (MQ=255)
                                                        aaaaGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGc                                                                                            >  1:280089/1‑149 (MQ=255)
                                                         aaaGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCg                                                                                           >  2:398810/1‑149 (MQ=255)
                                                            ggATCTGAATTTTCTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAg                                                                                        >  2:1892476/1‑149 (MQ=255)
                                                              aTCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGcc                                                                                      <  1:2175983/149‑1 (MQ=255)
                                                               tCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCa                                                                                     >  2:3760281/1‑149 (MQ=255)
                                                                cTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCAt                                                                                    <  1:4003145/149‑1 (MQ=255)
                                                                  gAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCt                                                                                  <  2:4554128/149‑1 (MQ=255)
                                                                   aaTTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTg                                                                                 <  1:1448533/149‑1 (MQ=255)
                                                                    aTTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAAGGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGa                                                                                >  2:3544550/1‑149 (MQ=255)
                                                                      tttttCTTCATGTGGGGCGATCTCTTATTTCACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGaaa                                                                              <  2:3567795/149‑1 (MQ=255)
                                                                      tttttCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAAGGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGaaa                                                                              >  2:13537/1‑149 (MQ=255)
                                                                          tcttcATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGGAaccac                                                                          >  1:4156988/1‑149 (MQ=255)
                                                                            ttcATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCAccc                                                                        >  2:873644/1‑149 (MQ=255)
                                                                             tcATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCAcccc                                                                       >  1:756540/1‑149 (MQ=255)
                                                                               aTGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCaa                                                                     >  1:3406871/1‑149 (MQ=255)
                                                                               aTGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCaa                                                                     >  2:4442578/1‑149 (MQ=255)
                                                                                tgtgGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAat                                                                    >  1:4440310/1‑149 (MQ=255)
                                                                                tgtgGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAat                                                                    >  1:2098374/1‑149 (MQ=255)
                                                                                  tgGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAatat                                                                  <  2:27667/149‑1 (MQ=255)
                                                                                  tgGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAatat                                                                  <  2:4208040/149‑1 (MQ=255)
                                                                                   ggggCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATAtg                                                                 >  2:385681/1‑149 (MQ=255)
                                                                                    gggCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATAtgt                                                                >  1:4558972/1‑149 (MQ=255)
                                                                                     ggCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATAtgtg                                                               <  1:1630435/149‑1 (MQ=255)
                                                                                      gCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGc                                                              >  1:2969540/1‑149 (MQ=255)
                                                                                      gCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGc                                                              <  1:944336/149‑1 (MQ=255)
                                                                                        gATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGAGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCa                                                            >  2:3718673/1‑149 (MQ=255)
                                                                                        gATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCa                                                            >  2:3140196/1‑149 (MQ=255)
                                                                                         aTCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAg                                                           <  1:2585307/149‑1 (MQ=255)
                                                                                          tctcTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGc                                                          <  1:2518306/149‑1 (MQ=255)
                                                                                          tctcTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGc                                                          >  1:2352426/1‑149 (MQ=255)
                                                                                           ctctTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGcc                                                         <  2:2933823/149‑1 (MQ=255)
                                                                                           ctctTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGcc                                                         <  1:3825987/149‑1 (MQ=255)
                                                                                              ttATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGt                                                      <  1:2333991/149‑1 (MQ=255)
                                                                                                 tttAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTAt                                                   <  2:1697991/149‑1 (MQ=255)
                                                                                                   tAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATcc                                                 <  1:1323745/149‑1 (MQ=255)
                                                                                                     aCAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAg                                               <  1:4308295/149‑1 (MQ=255)
                                                                                                        aaaTAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCg                                            >  2:1531829/1‑149 (MQ=255)
                                                                                                         aaTAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGc                                           >  2:2174405/1‑149 (MQ=255)
                                                                                                          aTAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGcc                                          >  2:2257025/1‑149 (MQ=255)
                                                                                                          aTAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGcc                                          <  2:4295221/149‑1 (MQ=255)
                                                                                                          aTAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGcc                                          >  1:3189523/1‑149 (MQ=255)
                                                                                                            aaCGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAg                                        >  2:3347700/1‑149 (MQ=255)
                                                                                                            aaCGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAg                                        >  2:2171896/1‑149 (MQ=255)
                                                                                                            aaCGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAg                                        <  1:546523/149‑1 (MQ=255)
                                                                                                                aTAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGgaga                                    >  1:1761328/1‑149 (MQ=255)
                                                                                                                aTAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGgaga                                    >  2:4667244/1‑149 (MQ=255)
                                                                                                                 tAATGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGAc                                   >  2:3325997/1‑149 (MQ=255)
                                                                                                                  aaTGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACa                                  >  1:2736208/1‑149 (MQ=255)
                                                                                                                   aTGCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACaa                                 >  1:3757420/1‑149 (MQ=255)
                                                                                                                     gCCCCACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAata                               >  1:1166616/1‑149 (MQ=255)
                                                                                                                       cccACCATCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATc                             <  2:1276616/149‑1 (MQ=255)
                                                                                                                        ccaccaTCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCg                            >  1:1076814/1‑149 (MQ=255)
                                                                                                                          accaTCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGcc                          >  2:1622187/1‑149 (MQ=255)
                                                                                                                            caTCCGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCg                        >  1:1880690/1‑149 (MQ=255)
                                                                                                                              tCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCt                      <  2:2891425/149‑1 (MQ=255)
                                                                                                                                cGCCAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTg                    >  2:3281227/1‑149 (MQ=255)
                                                                                                                                  ccAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGAt                  >  2:257684/1‑149 (MQ=255)
                                                                                                                                  ccAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGAt                  <  2:877250/149‑1 (MQ=255)
                                                                                                                                  ccAGTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGAt                  >  2:1948494/1‑149 (MQ=255)
                                                                                                                                     gTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGt               >  2:1242556/1‑149 (MQ=255)
                                                                                                                                     gTTAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGt               >  1:236787/1‑149 (MQ=255)
                                                                                                                                      ttAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTc              >  2:30802/1‑149 (MQ=255)
                                                                                                                                      ttAAACAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTc              >  2:4056942/1‑149 (MQ=255)
                                                                                                                                         aaCAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACt           >  2:2531880/1‑149 (MQ=255)
                                                                                                                                          aCAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTg          >  1:3599359/1‑149 (MQ=255)
                                                                                                                                           cAGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTgg         <  1:1028895/149‑1 (MQ=255)
                                                                                                                                            aGCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTggg        >  2:1424841/1‑149 (MQ=255)
                                                                                                                                             gCACATCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTgggg       >  1:285225/1‑149 (MQ=255)
                                                                                                                                               acaTCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGtt     >  1:2504679/1‑149 (MQ=255)
                                                                                                                                                caTCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTc    <  1:574635/149‑1 (MQ=255)
                                                                                                                                                 aTCGTCTTCCTGCGCGCCTGCGCCAATGTTCTGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCt   <  1:1621107/149‑1 (MQ=255)
                                                                                                                                                 aTCGTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCt   <  2:1576701/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGATTGTGCTGGCACTGGCGCTGGTGAGCCGTAAGAAAAAAGAAGCCGTCAGTGTAAAAAGGATCTGAATTTTTTCTTCATGTGGGGCGATCTCTTATTTAACAAAATAACGATAATGCCCCACCATCCGCCAGTTAAACAGCACATCTTCTTCCTGCGCGCCTGCGCCAATGTTATGTATCACCAGCGGCGTACCGTCGCGGGCGAAGCCATCTGAAACCACCCCAATATGTGCCAGCCCGTTATCCAGTCGCCAGGAGACAATATCGCCCGCTTGATAGTCACTGGGGTTCTT  >  CP009273/4126874‑4127168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑