Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,230,913 A→T 70.7% W424R (TGG→AGG)  xylE ← D‑xylose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,230,9130AT70.7% 514.3 / 286.0 283W424R (TGG→AGG) xylED‑xylose transporter
Reads supporting (aligned to +/- strand):  ref base A (36/47);  new base T (104/96);  total (140/143)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 283 total aligned reads displayed.

CTCAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGA  >  CP009273/4230767‑4231060
                                                                                                                                                  |                                                                                                                                                   
ctcAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTgg                                                                                                                                                   <  1:1065124/149‑1 (MQ=255)
 tcAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGga                                                                                                                                                  >  1:4173486/1‑149 (MQ=255)
 tcAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGga                                                                                                                                                  >  2:514789/1‑149 (MQ=255)
   aaGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTGTTTGTCCATCATCGGGAAGGTCCAGgaga                                                                                                                                                >  2:4500130/1‑149 (MQ=255)
     gggTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCAACATCGGGAAGGTCCTGGAGACg                                                                                                                                              >  1:3634429/1‑149 (MQ=255)
       gTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGaa                                                                                                                                            <  2:2127713/149‑1 (MQ=255)
       gTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGaa                                                                                                                                            >  1:1187076/1‑149 (MQ=255)
       gTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGaa                                                                                                                                            <  2:1450892/149‑1 (MQ=255)
          ttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAagta                                                                                                                                         <  1:390316/149‑1 (MQ=255)
          ttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAagta                                                                                                                                         <  2:462840/149‑1 (MQ=255)
          ttACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAagta                                                                                                                                         >  1:3046402/1‑149 (MQ=255)
             ccTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGtt                                                                                                                                      <  1:386681/149‑1 (MQ=255)
                ttGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCgc                                                                                                                                   <  1:292722/149‑1 (MQ=255)
                  ggTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCgcca                                                                                                                                 >  1:4394001/1‑149 (MQ=255)
                  ggTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCgcca                                                                                                                                 <  2:4241443/149‑1 (MQ=255)
                     ttCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCgccagcc                                                                                                                              <  1:1645827/149‑1 (MQ=255)
                     ttCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCgccagcc                                                                                                                              >  2:3714869/1‑149 (MQ=255)
                     ttCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCgccagcc                                                                                                                              <  1:1095536/149‑1 (MQ=255)
                         gggACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTg                                                                                                                          >  1:4002849/1‑149 (MQ=255)
                         gggACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTg                                                                                                                          >  1:4669999/1‑149 (MQ=255)
                          ggACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTgg                                                                                                                         <  1:3316490/149‑1 (MQ=255)
                          ggACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTgg                                                                                                                         <  1:980429/149‑1 (MQ=255)
                           gACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTggg                                                                                                                        >  2:3984201/1‑149 (MQ=255)
                            aCAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGgc                                                                                                                       >  1:4399640/1‑149 (MQ=255)
                                aTTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGgccgcc                                                                                                                   >  2:2493655/1‑149 (MQ=255)
                                    ccACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACcg                                                                                                               >  2:2411629/1‑149 (MQ=255)
                                     cacaTAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGACATGGGCCACCAGCCAGGAGATTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACcgc                                                                                                              <  1:1283177/149‑1 (MQ=255)
                                      acaTAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACcgcg                                                                                                             >  1:410072/1‑149 (MQ=255)
                                        aTAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGAt                                                                                                           <  1:2575208/149‑1 (MQ=255)
                                        aTAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGAt                                                                                                           >  2:4537514/1‑149 (MQ=255)
                                        aTAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGAt                                                                                                           <  2:3278284/149‑1 (MQ=255)
                                         tAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGAtt                                                                                                          >  1:3544530/1‑149 (MQ=255)
                                          aaaCAGTGCTGCCAGAACGCCCATACACCCGTAACTCCAGGAGGAGAAACCGTTGTGGAAATGGGCCACCCGCCAGGAGTTTTTGTCCATCCTCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTg                                                                                                         <  2:1028332/149‑1 (MQ=255)
                                                 gctgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAgcgc                                                                                                  >  2:4255195/1‑149 (MQ=255)
                                                   tgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCtt                                                                                                <  2:657991/149‑1 (MQ=255)
                                                   tgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCtt                                                                                                <  1:774697/149‑1 (MQ=255)
                                                   tgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCtt                                                                                                <  1:114357/149‑1 (MQ=255)
                                                   tgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCtt                                                                                                <  1:2286208/149‑1 (MQ=255)
                                                   tgcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCtt                                                                                                >  2:3568447/1‑149 (MQ=255)
                                                    gcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCttt                                                                                               <  1:3950732/149‑1 (MQ=255)
                                                    gcCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCttt                                                                                               <  2:3316661/149‑1 (MQ=255)
                                                     ccAGAACGCACATACAACCTTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCATGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTa                                                                                              <  2:1638130/149‑1 (MQ=255)
                                                        gAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCa                                                                                           >  2:2167367/1‑149 (MQ=255)
                                                        gAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCa                                                                                           >  2:1932201/1‑149 (MQ=255)
                                                            gCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGaa                                                                                       <  2:2709847/149‑1 (MQ=255)
                                                              ccATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATa                                                                                     <  1:402709/149‑1 (MQ=255)
                                                              ccATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATa                                                                                     <  2:3867257/149‑1 (MQ=255)
                                                                aTACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGc                                                                                   >  1:3158021/1‑149 (MQ=255)
                                                                 tACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCa                                                                                  >  1:3186897/1‑149 (MQ=255)
                                                                 tACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCa                                                                                  >  2:559291/1‑149 (MQ=255)
                                                                 tACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCa                                                                                  <  1:4056905/149‑1 (MQ=255)
                                                                   cAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCAtt                                                                                <  1:2422813/149‑1 (MQ=255)
                                                                       cGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCggg                                                                            >  1:276865/1‑149 (MQ=255)
                                                                        gTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGa                                                                           <  1:3722072/149‑1 (MQ=255)
                                                                         tAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGac                                                                          >  1:1581691/1‑148 (MQ=255)
                                                                         tAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGaa                                                                          <  1:2626900/149‑1 (MQ=255)
                                                                         tAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGaa                                                                          >  2:284766/1‑149 (MQ=255)
                                                                           aaTCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGa                                                                        >  1:2164165/1‑149 (MQ=255)
                                                                            aTCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGAt                                                                       <  1:191003/149‑1 (MQ=255)
                                                                              ccAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGAttt                                                                     <  2:3782486/149‑1 (MQ=255)
                                                                               cAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTc                                                                    <  2:3877567/149‑1 (MQ=255)
                                                                                aGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTcc                                                                   <  2:791478/149‑1 (MQ=255)
                                                                                aGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTcc                                                                   <  1:4472118/149‑1 (MQ=255)
                                                                                 gTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCg                                                                  >  1:2584680/1‑149 (MQ=255)
                                                                                   aGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAc                                                                <  1:584763/149‑1 (MQ=255)
                                                                                    ggAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAca                                                               <  1:2321526/149‑1 (MQ=255)
                                                                                     gagaAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCTTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGAcag                                                              >  1:4592613/1‑149 (MQ=255)
                                                                                          aCCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTa                                                         >  2:684217/1‑149 (MQ=255)
                                                                                          aCCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTa                                                         >  2:3023955/1‑149 (MQ=255)
                                                                                           ccGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTAc                                                        <  2:2565801/149‑1 (MQ=255)
                                                                                                 tgGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCa                                                  <  2:3634429/149‑1 (MQ=255)
                                                                                                  ggAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCAt                                                 <  2:1600110/149‑1 (MQ=255)
                                                                                                  ggAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCAt                                                 <  2:3935584/149‑1 (MQ=255)
                                                                                                   gAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATa                                                >  1:1345846/1‑149 (MQ=255)
                                                                                                    aaaTGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATAc                                               <  2:2389301/149‑1 (MQ=255)
                                                                                                     aaTGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATAcc                                              <  1:2172987/149‑1 (MQ=255)
                                                                                                       tGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCgg                                            >  1:1410307/1‑149 (MQ=255)
                                                                                                        gggCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGa                                           >  2:1748190/1‑149 (MQ=255)
                                                                                                              ccagccagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAg                                     >  2:3131529/1‑149 (MQ=255)
                                                                                                                  ccagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACa                                 >  1:664142/1‑149 (MQ=255)
                                                                                                                  ccagGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACa                                 <  2:1806221/149‑1 (MQ=255)
                                                                                                                    agGAGTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATg                               >  2:1799495/1‑149 (MQ=255)
                                                                                                                        gTTTTTGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCaa                           >  2:1280684/1‑149 (MQ=255)
                                                                                                                        gTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCaa                           >  2:2974495/1‑149 (MQ=255)
                                                                                                                         tttttGTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCaaa                          <  1:2007385/149‑1 (MQ=255)
                                                                                                                           tttGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAgg                        <  1:3578854/149‑1 (MQ=255)
                                                                                                                              gTCCATCATCGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAggcgg                     <  2:3522885/149‑1 (MQ=255)
                                                                                                                                ccATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCa                   <  2:1890485/149‑1 (MQ=255)
                                                                                                                                   tcatcGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACa                <  2:1187076/149‑1 (MQ=255)
                                                                                                                                    catcGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAatat               >  2:536817/1‑149 (MQ=255)
                                                                                                                                    catcGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACAt               <  2:4095218/149‑1 (MQ=255)
                                                                                                                                      tcGGGAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAg             >  1:4519213/1‑149 (MQ=255)
                                                                                                                                         ggAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAAc          >  1:617616/1‑149 (MQ=255)
                                                                                                                                         ggAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAAc          >  1:2994178/1‑149 (MQ=255)
                                                                                                                                         ggAAGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAAc          <  2:606809/149‑1 (MQ=255)
                                                                                                                                           aaGGTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAg        >  2:2674577/1‑149 (MQ=255)
                                                                                                                                             ggTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCa      >  1:872655/1‑149 (MQ=255)
                                                                                                                                             ggTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCa      <  1:4317553/149‑1 (MQ=255)
                                                                                                                                             ggTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCa      <  1:1878140/149‑1 (MQ=255)
                                                                                                                                              gTCCTGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCAt     >  1:2693204/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTCAAGGGTTTTACCTTTGGTTTCCGGGACAAATTTCCACATAAACAGTGCTGCCAGAACGCCCATACAACCGTAAATCCAGTAGGAGAAACCGTTGTGGAAATGGGCCACCAGCCAGGAGTTTTTGTCCATCATCGGGAAGGTCCAGGAGACGAAGTAGTTCGCCAGCCACTGGGCCGCCACCGCGATTGCCAGCGCTTTACCACGAATAGCATTCGGGAAGATTTCCGACAGCAGTACCCAGCATACCGGACCCCAGGACATGGCAAAGGCGGCAACATAGAACAGCATCGA  >  CP009273/4230767‑4231060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑