BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,258,903	G→T	5.6%	S13Y (TCT→TAT)	yjbS ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,258,903	0	G	T	5.6%	981.2 / 27.5	258	S13Y (TCT→TAT)	yjbS	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (125/117); new base T (8/6); total (133/125)
Fisher's exact test for biased strand distribution p-value = 7.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

Only 100 of 262 total aligned reads displayed.

AAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTATCATT  >  CP009273/4258756‑4259051

aaaaaCTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGa                                                                                                                                                     >  2:1349428/1‑149 (MQ=255)

        tACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGttt                                                                                                                                             <  2:741096/149‑1 (MQ=255)

         aCTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGtttt                                                                                                                                            <  1:4487271/149‑1 (MQ=255)

         aCTTGCAAGAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGtttt                                                                                                                                            <  1:3169140/149‑1 (MQ=255)

           ttGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTttgt                                                                                                                                          >  2:2103919/1‑149 (MQ=255)

            tGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTttgtt                                                                                                                                         <  1:2678090/149‑1 (MQ=255)

            tGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTttgtt                                                                                                                                         <  1:1290744/149‑1 (MQ=255)

            tGCAATAGAACTTGATACTTCTTCTCCAGGTATAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTttgtt                                                                                                                                         <  2:3523519/149‑1 (MQ=255)

               aaTAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCt                                                                                                                                      <  2:3889351/149‑1 (MQ=255)

                aTAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTa                                                                                                                                     >  2:1646518/1‑149 (MQ=255)

                aTAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTa                                                                                                                                     <  1:4187252/149‑1 (MQ=255)

                 tAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTAt                                                                                                                                    <  1:3827252/149‑1 (MQ=255)

                  aGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTAtt                                                                                                                                   >  2:3157430/1‑149 (MQ=255)

                   gAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTc                                                                                                                                  <  1:2978711/149‑1 (MQ=255)

                    aaCTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTca                                                                                                                                 >  1:507048/1‑149 (MQ=255)

                       ttGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGAATAAGAGATATCTTTATTATTTATTGCATCTATCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTAACat                                                                                                                              >  1:3768198/1‑149 (MQ=255)

                        tGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACata                                                                                                                             <  1:2142034/149‑1 (MQ=255)

                          aTACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATg                                                                                                                           >  1:1026884/1‑149 (MQ=255)

                           tACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTcac                                                                                                                                <  2:3944115/143‑1 (MQ=255)

                           tACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGa                                                                                                                          >  1:2719175/1‑149 (MQ=255)

                             cttcttCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGata                                                                                                                        >  1:276034/1‑149 (MQ=255)

                               tcttctCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGatata                                                                                                                      <  1:884230/149‑1 (MQ=255)

                               tcttctCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGatata                                                                                                                      <  1:2450430/149‑1 (MQ=255)

                                cttctCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGatatat                                                                                                                     <  1:4322234/149‑1 (MQ=255)

                                 ttctCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATAtt                                                                                                                    <  2:1066755/149‑1 (MQ=255)

                                 ttctCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATAtt                                                                                                                    >  2:174515/1‑149 (MQ=255)

                                    tcCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCat                                                                                                                 >  1:4020492/1‑149 (MQ=255)

                                         gTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATatcat                                                                                                            >  1:1009406/1‑149 (MQ=255)

                                          tCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATACAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATatcatc                                                                                                           <  2:3120506/149‑1 (MQ=255)

                                          tCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATatcatc                                                                                                           >  2:4168577/1‑149 (MQ=255)

                                          tCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATatcatc                                                                                                           >  2:3382885/1‑149 (MQ=255)

                                              aaaaaTCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCAtct                                                                                                       >  2:2665888/1‑149 (MQ=255)

                                                  aTCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATATACAt                                                                                                   >  1:2876443/1‑149 (MQ=255)

                                                  aTCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCAt                                                                                                   <  1:3992553/149‑1 (MQ=255)

                                                      tttGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATcgcg                                                                                               <  2:2142229/149‑1 (MQ=255)

                                                       ttGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGa                                                                                              >  2:2355948/1‑149 (MQ=255)

                                                       ttGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGa                                                                                              <  1:698157/149‑1 (MQ=255)

                                                            aTTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGtaat                                                                                         >  1:4390571/1‑149 (MQ=255)

                                                                   aCTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTa                                                                                  >  2:2234168/1‑149 (MQ=255)

                                                                    cTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTaa                                                                                 >  2:1979252/1‑149 (MQ=255)

                                                                     tgtgTTTATATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAAt                                                                                <  2:4670974/149‑1 (MQ=255)

                                                                      gtgtTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATc                                                                               <  2:2018805/149‑1 (MQ=255)

                                                                       tgtTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCa                                                                              <  2:2370896/149‑1 (MQ=255)

                                                                           tCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTATTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATTATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCAt                                                                          >  1:3413790/1‑149 (MQ=255)

                                                                           tCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCAt                                                                          >  1:891220/1‑149 (MQ=255)

                                                                             tATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGTGTAATAAAATTAATCACCATTg                                                                        >  1:2620421/1‑149 (MQ=255)

                                                                               tGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgta                                                                      >  2:1970589/1‑149 (MQ=255)

                                                                               tGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgta                                                                      <  2:668583/149‑1 (MQ=255)

                                                                               tGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgta                                                                      <  2:3584470/149‑1 (MQ=255)

                                                                                gTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtag                                                                     >  1:148502/1‑149 (MQ=255)

                                                                                  ataAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtaggg                                                                   >  1:1393980/1‑149 (MQ=255)

                                                                                   taAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtagggt                                                                  <  2:3672704/149‑1 (MQ=255)

                                                                                    aaaaaGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAGTTAATCACCATTgtagggta                                                                 >  2:1521590/1‑149 (MQ=255)

                                                                                    aaaaaGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTgtagggta                                                                 >  2:4219642/1‑149 (MQ=255)

                                                                                         gAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAggggg                                                            <  1:796647/149‑1 (MQ=255)

                                                                                          aaaTCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGc                                                           <  1:332477/149‑1 (MQ=255)

                                                                                           aaTCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCt                                                          <  1:2010558/149‑1 (MQ=255)

                                                                                             tCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTgg                                                        >  2:834032/1‑149 (MQ=255)

                                                                                             tCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTgg                                                        <  1:2294409/149‑1 (MQ=255)

                                                                                             tCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTgg                                                        <  2:1818681/149‑1 (MQ=255)

                                                                                             tCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTgg                                                        <  1:725780/149‑1 (MQ=255)

                                                                                               tttGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTc                                                      >  2:753059/1‑149 (MQ=255)

                                                                                               tttGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTc                                                      <  1:2950050/149‑1 (MQ=255)

                                                                                                  ggCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAAt                                                   >  2:1870740/1‑149 (MQ=255)

                                                                                                  ggCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAAt                                                   <  2:611140/149‑1 (MQ=255)

                                                                                                   gCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATc                                                  >  1:3601792/1‑149 (MQ=255)

                                                                                                    cATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCa                                                 >  2:2525225/1‑149 (MQ=255)

                                                                                                          agaTATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAatc                                           >  1:1883496/1‑149 (MQ=255)

                                                                                                           gaTATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAatca                                          <  1:3933003/149‑1 (MQ=255)

                                                                                                           gaTATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAatca                                          <  1:1598045/149‑1 (MQ=255)

                                                                                                            atatCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAatcat                                         >  1:3874442/1‑149 (MQ=255)

                                                                                                              atCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTCTTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCAGTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCt                                       >  2:1417062/1‑149 (MQ=255)

                                                                                                              atCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAACTCATCt                                       >  2:2798907/1‑149 (MQ=255)

                                                                                                              atCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCt                                       >  2:3256199/1‑149 (MQ=255)

                                                                                                                 tttATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTta                                    <  2:4307969/149‑1 (MQ=255)

                                                                                                                 tttATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTta                                    <  1:4614960/149‑1 (MQ=255)

                                                                                                                     ttattTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATaaa                                >  1:1180289/1‑149 (MQ=255)

                                                                                                                       attTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACt                              <  2:4157347/149‑1 (MQ=255)

                                                                                                                        tttAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACtt                             <  2:447519/149‑1 (MQ=255)

                                                                                                                        tttAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACtt                             <  2:245181/149‑1 (MQ=255)

                                                                                                                         ttAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTc                            >  2:1852969/1‑149 (MQ=255)

                                                                                                                           aGTGCATCTAGCTCAACAGGTAAAAATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGa                          <  2:4332636/149‑1 (MQ=255)

                                                                                                                           aGTGCATCTAGCTCAACAGGTAAAAATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGa                          <  2:2287498/149‑1 (MQ=255)

                                                                                                                            gTGCATCTAGCTCAACAGGTAAATATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGAt                         <  1:1646518/149‑1 (MQ=255)

                                                                                                                              gCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTg                       <  2:588389/149‑1 (MQ=255)

                                                                                                                                    aGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTg                 <  2:3003717/149‑1 (MQ=255)

                                                                                                                                    aGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTg                 <  2:2889322/149‑1 (MQ=255)

                                                                                                                                     gCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGt                <  1:3027769/149‑1 (MQ=255)

                                                                                                                                     gCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGt                <  1:4404360/149‑1 (MQ=255)

                                                                                                                                     gCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGt                <  1:1746026/149‑1 (MQ=255)

                                                                                                                                     gCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGt                >  2:4564023/1‑149 (MQ=255)

                                                                                                                                         aaCAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCAGTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATg            >  2:3745164/1‑149 (MQ=255)

                                                                                                                                            aGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTTTTTTTGTAATGCTg         >  1:544012/1‑149 (MQ=255)

                                                                                                                                            aGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTg         >  1:1185219/1‑149 (MQ=255)

                                                                                                                                                aaaTATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTATc     <  2:1947079/149‑1 (MQ=255)

                                                                                                                                                 aaGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTATCa    >  2:1543962/1‑149 (MQ=255)

                                                                                                                                                  aGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAATCTTCGATTGTTTTTGTAATGCTGTATctt   >  2:3454459/1‑147 (MQ=255)

                                                                                                                                                   gATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTATCAtt  >  1:3258605/1‑149 (MQ=255)

                                                                                                                                                   gATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTATCAtt  <  1:1822171/149‑1 (MQ=255)

                                                                                                                                                   gATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGATGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTATCAtt  <  1:35881/149‑1 (MQ=255)

AAAAACTTTACTTGCAATAGAACTTGATACTTCTTCTCCAGGTCTAAAAAATCTTTTGCAATTTTTAACTGTGTTTCTATGTATAAAAAGAAATCTTTGGCATAAGAGATATCTTTATTATTTAGTGCATCTAGCTCAACAGGTAAAGATGTCGTTTTGTTGCTATTCACATATGATATATTCATATCATCATCTCCATCGCGAGTAATAAAATTAATCACCATTGTAGGGTAGGGGGCTGGTCAATCAGAAATCATCTTTATAAACTTCGATTGTTTTTGTAATGCTGTATCATT  >  CP009273/4258756‑4259051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`