BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,374,805	G→T	5.9%	G384C (GGT→TGT)	yjeM →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,374,805	0	G	T	5.9%	1041.2 / 32.5	269	G384C (GGT→TGT)	yjeM	putative transporter
Reads supporting (aligned to +/- strand): ref base G (133/120); new base T (6/10); total (139/130)
Fisher's exact test for biased strand distribution p-value = 3.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 276 total aligned reads displayed.

ACCGTTGAAAGCCATCATCCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTC  >  CP009273/4374657‑4374953

    ttGAAAGCCATCATCCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTAc                                                                                                                                                  >  1:1037329/1‑149 (MQ=255)

    ttGAAAGCCATCATCCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTAc                                                                                                                                                  <  1:786572/149‑1 (MQ=255)

    ttGAAAGCCATCATCCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTAc                                                                                                                                                  >  1:3373645/1‑149 (MQ=255)

    ttGAAAGCCATCATCCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGACGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTAc                                                                                                                                                  >  2:2945243/1‑149 (MQ=255)

       aaaGCCATCATCCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGc                                                                                                                                               >  2:2704986/1‑149 (MQ=255)

       aaaGCCATCATCCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGc                                                                                                                                               >  2:1968059/1‑149 (MQ=255)

          gCCATCATCCAGGGGACGCCGAAAGCATTGTGTCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATc                                                                                                                                            <  1:3826264/149‑1 (MQ=255)

            catcatCCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCgg                                                                                                                                          <  1:244676/149‑1 (MQ=255)

             atcatcCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGc                                                                                                                                         >  1:893026/1‑149 (MQ=255)

              tcatcCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCg                                                                                                                                        >  1:2976668/1‑149 (MQ=255)

              tcatcCAGGGGACGCCGAAAGCAGTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCg                                                                                                                                        >  2:1155817/1‑149 (MQ=255)

                 tcCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTc                                                                                                                                     <  2:1764225/149‑1 (MQ=255)

                   cAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCtt                                                                                                                                   <  2:2860332/149‑1 (MQ=255)

                     ggggACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTa                                                                                                                                 >  2:4327623/1‑149 (MQ=255)

                          cGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAg                                                                                                                            <  1:4148593/149‑1 (MQ=255)

                            ccGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCTGTACCGCATCGGCGTTCTTTAACAAGCt                                                                                                                          <  1:576236/149‑1 (MQ=255)

                             cGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTg                                                                                                                         >  1:244272/1‑149 (MQ=255)

                             cGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTg                                                                                                                         >  2:4250613/1‑149 (MQ=255)

                             cGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTg                                                                                                                         >  2:4068717/1‑149 (MQ=255)

                             cGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTg                                                                                                                         <  1:1519153/149‑1 (MQ=255)

                             cGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTg                                                                                                                         >  1:4092855/1‑149 (MQ=255)

                              gAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGa                                                                                                                        <  1:1204812/149‑1 (MQ=255)

                              gAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGa                                                                                                                        <  2:1577787/149‑1 (MQ=255)

                               aaaGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGAc                                                                                                                       <  1:1449888/149‑1 (MQ=255)

                               aaaGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGAc                                                                                                                       >  2:1317094/1‑149 (MQ=255)

                               aaaGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGAc                                                                                                                       <  1:1977965/149‑1 (MQ=255)

                                 aGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGc                                                                                                                     >  2:2354815/1‑149 (MQ=255)

                                 aGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGc                                                                                                                     >  1:4083987/1‑149 (MQ=255)

                                             ggAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACg                                                                                                         >  2:766789/1‑149 (MQ=255)

                                             ggAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACg                                                                                                         >  2:3470960/1‑149 (MQ=255)

                                                aCCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACgtgt                                                                                                      <  1:4343777/149‑1 (MQ=255)

                                                 ccGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTc                                                                                                     <  2:381475/149‑1 (MQ=255)

                                                 ccGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTc                                                                                                     <  2:1445613/149‑1 (MQ=255)

                                                 ccGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTc                                                                                                     <  2:4518124/149‑1 (MQ=255)

                                                 ccGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTc                                                                                                     <  1:1695408/149‑1 (MQ=255)

                                                  cGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCt                                                                                                    <  1:3671666/149‑1 (MQ=255)

                                                   gATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTa                                                                                                   >  1:3739593/1‑149 (MQ=255)

                                                      gACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGa                                                                                                >  1:2509768/1‑149 (MQ=255)

                                                      gACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCAGACGCTGATGGCGAACGTGTCTAtta                                                                                                >  1:4297689/1‑147 (MQ=255)

                                                          cgcCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCt                                                                                            <  2:3975553/149‑1 (MQ=255)

                                                           gcCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCTGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCtt                                                                                           >  2:734037/1‑149 (MQ=255)

                                                                 aTGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCTGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTAc                                                                                     >  1:1593534/1‑149 (MQ=255)

                                                                  tGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTAcc                                                                                    <  2:3980946/149‑1 (MQ=255)

                                                                     gATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCTGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGt                                                                                 <  1:258928/149‑1 (MQ=255)

                                                                      aTGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGtt                                                                                <  1:1780685/149‑1 (MQ=255)

                                                                        ggggATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTcc                                                                              >  1:3960883/1‑149 (MQ=255)

                                                                        ggggATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTcc                                                                              >  1:1388360/1‑149 (MQ=255)

                                                                        ggggATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTcc                                                                              >  2:1710549/1‑149 (MQ=255)

                                                                         gggATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCt                                                                             >  1:732687/1‑149 (MQ=255)

                                                                           gATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTcg                                                                           >  2:3418322/1‑149 (MQ=255)

                                                                            aTGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGTCGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTcgc                                                                          <  2:813145/149‑1 (MQ=255)

                                                                                cGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTg                                                                      <  2:2534033/149‑1 (MQ=255)

                                                                                  tCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGTTGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGc                                                                    >  2:1231989/1‑149 (MQ=255)

                                                                                   cTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCt                                                                   <  2:2050842/149‑1 (MQ=255)

                                                                                    tATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCTGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCtt                                                                  >  2:2232327/1‑149 (MQ=255)

                                                                                    tATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCtt                                                                  >  2:4564938/1‑149 (MQ=255)

                                                                                     aTCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCttt                                                                 >  2:1412964/1‑149 (MQ=255)

                                                                                     aTCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCttt                                                                 >  2:3748932/1‑149 (MQ=255)

                                                                                       cGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTcc                                                               <  2:796122/149‑1 (MQ=255)

                                                                                       cGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTcc                                                               >  2:3517641/1‑149 (MQ=255)

                                                                                        gCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTccc                                                              >  1:278891/1‑149 (MQ=255)

                                                                                           aTGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGtt                                                           >  2:3295739/1‑149 (MQ=255)

                                                                                            tgtgGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTc                                                          <  1:1242538/149‑1 (MQ=255)

                                                                                            tgtgGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTc                                                          <  2:4217741/149‑1 (MQ=255)

                                                                                               ggATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCttt                                                       >  2:410214/1‑149 (MQ=255)

                                                                                                  tGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTaaa                                                    <  2:182059/149‑1 (MQ=255)

                                                                                                  tGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTaaa                                                    <  2:1037329/149‑1 (MQ=255)

                                                                                                   gCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAg                                                   >  1:532819/1‑149 (MQ=255)

                                                                                                    cAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGc                                                  >  1:536307/1‑149 (MQ=255)

                                                                                                      gTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCAc                                                >  2:313554/1‑149 (MQ=255)

                                                                                                       tGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACg                                               >  2:2447703/1‑149 (MQ=255)

                                                                                                       tGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACg                                               <  2:498480/149‑1 (MQ=255)

                                                                                                           ggttggttACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAg                                           >  2:4386148/1‑149 (MQ=255)

                                                                                                            gttggttACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAgg                                          >  1:3204563/1‑149 (MQ=255)

                                                                                                            gttggttACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAgg                                          >  1:4443524/1‑149 (MQ=255)

                                                                                                              tggttACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGAt                                        <  2:3071565/149‑1 (MQ=255)

                                                                                                              tggttACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGAt                                        <  1:2703493/149‑1 (MQ=255)

                                                                                                               ggttACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCTGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGAtc                                       <  2:4091693/149‑1 (MQ=255)

                                                                                                               ggttACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGAtc                                       >  2:1533348/1‑149 (MQ=255)

                                                                                                               ggttACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGAtc                                       >  2:3870474/1‑149 (MQ=255)

                                                                                                                gttACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGAtct                                      >  2:4543398/1‑149 (MQ=255)

                                                                                                                    cTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGAc                                  <  1:639436/149‑1 (MQ=255)

                                                                                                                        cTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGAc                              >  2:320747/1‑149 (MQ=255)

                                                                                                                         ttCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGAcc                             <  1:3080701/149‑1 (MQ=255)

                                                                                                                          tCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCg                            >  1:1313869/1‑149 (MQ=255)

                                                                                                                          tCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCg                            >  1:3649985/1‑149 (MQ=255)

                                                                                                                              ccTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTtg                        <  1:4226400/149‑1 (MQ=255)

                                                                                                                              ccTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTtg                        <  1:1891553/149‑1 (MQ=255)

                                                                                                                               ctgctgGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTtgt                       >  2:86641/1‑149 (MQ=255)

                                                                                                                               ctgctgGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTtgt                       >  1:526281/1‑149 (MQ=255)

                                                                                                                                 gctgGTTTCGTTTGGTGGCTGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGa                     <  2:3549783/149‑1 (MQ=255)

                                                                                                                                 gctgGTTTCGTTTGGTGGCTGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGa                     <  2:1212433/149‑1 (MQ=255)

                                                                                                                                   tgGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGAtt                   <  1:1182461/149‑1 (MQ=255)

                                                                                                                                      tttCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACATGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTc                >  1:1124570/1‑149 (MQ=255)

                                                                                                                                          gTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCaaaa            <  1:3282577/149‑1 (MQ=255)

                                                                                                                                              ggtggCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGGTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCa        >  1:3577520/1‑149 (MQ=255)

                                                                                                                                                tggCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCAtt      <  1:4543398/149‑1 (MQ=255)

                                                                                                                                                tggCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCAtt      <  1:2137394/149‑1 (MQ=255)

                                                                                                                                                    ggTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTc  <  2:1913696/149‑1 (MQ=255)

                                                                                                                                                    ggTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTc  <  1:2324796/149‑1 (MQ=255)

ACCGTTGAAAGCCATCATCCAGGGGACGCCGAAAGCATTGTGGCCGGAACCGATGACGCGCCTGAATGCGATGGGGATGCCGTCTATCGCCATGTGGATGCAGTGCGGGTTGGTTACTGTCTTCATCCTGCTGGTTTCGTTTGGTGGCGGTACCGCATCGGCGTTCTTTAACAAGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTC  >  CP009273/4374657‑4374953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`