BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,458,609	G→T	86.9%	D390Y (GAT→TAT)	mgtA →	magnesium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,458,609	0	G	T	86.9%	919.1 / 136.0	328	D390Y (GAT→TAT)	mgtA	magnesium transporter
Reads supporting (aligned to +/- strand): ref base G (24/19); new base T (159/126); total (183/145)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 337 total aligned reads displayed.

GGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAA  >  CP009273/4458464‑4458755

ggCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCtata                                                                                                                                                 <  2:763712/149‑1 (MQ=255)

ggCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCtata                                                                                                                                                 <  2:4635924/149‑1 (MQ=255)

ggCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCtata                                                                                                                                                 >  2:460645/1‑149 (MQ=255)

ggCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCtata                                                                                                                                                 <  1:2924268/149‑1 (MQ=255)

 gcgcgcGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGatat                                                                                                                                                >  1:590494/1‑149 (MQ=255)

   gcgcgGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATAtct                                                                                                                                              >  1:1348412/1‑149 (MQ=255)

     gcggcgCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTcc                                                                                                                                            <  1:2981083/149‑1 (MQ=255)

       ggcgCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCgg                                                                                                                                          <  2:1220927/149‑1 (MQ=255)

         cgcAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTa                                                                                                                                        <  1:2302589/149‑1 (MQ=255)

          gcAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTaa                                                                                                                                       <  2:1370137/149‑1 (MQ=255)

            aGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTaaaa                                                                                                                                     >  2:2180422/1‑149 (MQ=255)

                aaGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAg                                                                                                                                 >  2:190012/1‑149 (MQ=255)

                aaGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAg                                                                                                                                 <  1:3593773/149‑1 (MQ=255)

                aaGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAg                                                                                                                                 <  1:2236564/149‑1 (MQ=255)

                aaGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAg                                                                                                                                 <  2:212838/149‑1 (MQ=255)

                 aGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGc                                                                                                                                >  2:213694/1‑149 (MQ=255)

                 aGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGc                                                                                                                                >  2:4096522/1‑149 (MQ=255)

                    tGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGaa                                                                                                                             >  1:4600336/1‑149 (MQ=255)

                    tGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGaa                                                                                                                             >  1:1054186/1‑149 (MQ=255)

                     gTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAAc                                                                                                                            >  1:3876074/1‑149 (MQ=255)

                        gAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAAcgcg                                                                                                                         >  2:159092/1‑149 (MQ=255)

                         aaaCAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGt                                                                                                                        >  2:2089453/1‑149 (MQ=255)

                         aaaCAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGt                                                                                                                        >  1:3808018/1‑149 (MQ=255)

                          aaCAGAAAGTGCTCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtg                                                                                                                       <  2:2383902/149‑1 (MQ=255)

                          aaCAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtg                                                                                                                       >  1:1731040/1‑149 (MQ=255)

                           aCAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtgc                                                                                                                      >  2:3827339/1‑149 (MQ=255)

                           aCAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGtgc                                                                                                                      <  1:2955619/149‑1 (MQ=255)

                               aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCa                                                                                                                  <  2:82627/149‑1 (MQ=255)

                               aaaGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCa                                                                                                                  >  1:4320037/1‑149 (MQ=255)

                                  gTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAg                                                                                                               >  1:3062556/1‑149 (MQ=255)

                                     aTCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAgcgc                                                                                                            >  1:1578825/1‑149 (MQ=255)

                                     aTCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAgcgc                                                                                                            >  1:4041922/1‑149 (MQ=255)

                                      tCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGcgcg                                                                                                           >  1:1172696/1‑149 (MQ=255)

                                        gTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTg                                                                                                         <  2:2954176/149‑1 (MQ=255)

                                          cAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGt                                                                                                       >  2:3956535/1‑149 (MQ=255)

                                          cAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGt                                                                                                       <  1:1865253/149‑1 (MQ=255)

                                            aaCATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGACTCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTg                                                                                                     >  2:627626/1‑149 (MQ=255)

                                            aaCATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTg                                                                                                     <  1:2530635/149‑1 (MQ=255)

                                              cATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGaa                                                                                                   <  1:2886863/149‑1 (MQ=255)

                                               aTCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAAc                                                                                                  <  1:2331532/149‑1 (MQ=255)

                                                tCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACa                                                                                                 >  1:672768/1‑149 (MQ=255)

                                                  tGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGt                                                                                               <  2:3178872/149‑1 (MQ=255)

                                                       gCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTa                                                                                          >  1:4628786/1‑149 (MQ=255)

                                                          aTTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCa                                                                                       <  1:3300774/149‑1 (MQ=255)

                                                          aTTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCa                                                                                       <  1:37579/149‑1 (MQ=255)

                                                             cAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGAc                                                                                    <  1:1452469/149‑1 (MQ=255)

                                                             cAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGAc                                                                                    <  2:1969432/149‑1 (MQ=255)

                                                              aGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGAcc                                                                                   >  1:1894546/1‑149 (MQ=255)

                                                               gAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCg                                                                                  >  1:3335932/1‑149 (MQ=255)

                                                                aaCTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCgg                                                                                 <  2:4504335/149‑1 (MQ=255)

                                                                 aCTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGTTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGa                                                                                <  2:1080071/149‑1 (MQ=255)

                                                                 aCTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGa                                                                                >  1:2728242/1‑149 (MQ=255)

                                                                 aCTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGa                                                                                <  1:190012/149‑1 (MQ=255)

                                                                  cTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGAc                                                                               >  1:4138053/1‑149 (MQ=255)

                                                                  cTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGAc                                                                               >  1:4017333/1‑149 (MQ=255)

                                                                  cTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGAc                                                                               >  2:1289821/1‑149 (MQ=255)

                                                                      ggCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTaa                                                                           >  2:3706263/1‑149 (MQ=255)

                                                                      ggCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTaa                                                                           <  2:4057196/149‑1 (MQ=255)

                                                                           aaTGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAAcc                                                                      >  2:3258268/1‑149 (MQ=255)

                                                                            aTGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCt                                                                     >  1:3209535/1‑149 (MQ=255)

                                                                            aTGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCt                                                                     <  2:4136761/149‑1 (MQ=255)

                                                                               gATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCt                                                                  <  2:1479983/149‑1 (MQ=255)

                                                                                   ttCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGAt                                                              <  1:2399950/149‑1 (MQ=255)

                                                                                     cTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATAc                                                            >  2:1869505/1‑149 (MQ=255)

                                                                                     cTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATAc                                                            <  2:272958/149‑1 (MQ=255)

                                                                                       gtgCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAg                                                          >  1:2102951/1‑149 (MQ=255)

                                                                                       gtgCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAg                                                          <  2:3436844/149‑1 (MQ=255)

                                                                                          cACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCgg                                                       >  1:1266042/1‑149 (MQ=255)

                                                                                           aCTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGt                                                      >  1:1724056/1‑149 (MQ=255)

                                                                                             tGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGc                                                    <  2:2483453/149‑1 (MQ=255)

                                                                                                  aaaCCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGaa                                               >  1:3605751/1‑149 (MQ=255)

                                                                                                  aaaCCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGaa                                               >  1:4658749/1‑149 (MQ=255)

                                                                                                   aaCCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAg                                              <  1:733068/149‑1 (MQ=255)

                                                                                                    aCCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAgg                                             >  1:1498939/1‑149 (MQ=255)

                                                                                                      cGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTa                                           >  1:863466/1‑149 (MQ=255)

                                                                                                        gCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACg                                         <  1:2107124/149‑1 (MQ=255)

                                                                                                               gACGCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAag                                  <  2:3539967/149‑1 (MQ=255)

                                                                                                                  gCAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGt                               <  1:845975/149‑1 (MQ=255)

                                                                                                                   cAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTc                              >  2:3021524/1‑149 (MQ=255)

                                                                                                                   cAGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTc                              <  1:3701067/149‑1 (MQ=255)

                                                                                                                    aGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCa                             >  2:811007/1‑149 (MQ=255)

                                                                                                                    aGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCa                             >  2:2312996/1‑149 (MQ=255)

                                                                                                                    aGGATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCa                             <  2:40228/149‑1 (MQ=255)

                                                                                                                     ggATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAg                            >  2:4596161/1‑149 (MQ=255)

                                                                                                                      gATAAAATTGTGCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAgc                           <  1:975846/149‑1 (MQ=255)

                                                                                                                            aTTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGcgctc                     <  2:1663171/149‑1 (MQ=255)

                                                                                                                              tgtgCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGcgctcgc                   <  2:434454/149‑1 (MQ=255)

                                                                                                                              tgtgCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGcgctcgc                   <  2:2898062/149‑1 (MQ=255)

                                                                                                                               gtgCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGcgctcgct                  >  1:4432527/1‑149 (MQ=255)

                                                                                                                               gtgCTGGAGAATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGcgctcgct                  >  1:1395867/1‑149 (MQ=255)

                                                                                                                                   tGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGcc              >  1:3115007/1‑149 (MQ=255)

                                                                                                                                      agaATCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGt           <  1:3956585/149‑1 (MQ=255)

                                                                                                                                        aaTCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCg         >  2:2051392/1‑149 (MQ=255)

                                                                                                                                         aTCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGt        >  1:2666566/1‑149 (MQ=255)

                                                                                                                                          tCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGtt       >  1:2253589/1‑149 (MQ=255)

                                                                                                                                          tCATACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGtt       <  1:4360940/149‑1 (MQ=255)

                                                                                                                                            aTACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTgg     >  2:582036/1‑149 (MQ=255)

                                                                                                                                            aTACCTATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTgg     >  1:1643563/1‑149 (MQ=255)

                                                                                                                                            aTACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTgg     >  2:2783704/1‑149 (MQ=255)

                                                                                                                                               ccGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCaa  >  1:35554/1‑149 (MQ=255)

GGCGCGCGGCGCAGTAAAGCTGTCGAAACAGAAAGTGATCGTCAAACATCTGGATGCTATTCAGAACTTTGGCGCAATGGATATTCTGTGCACTGATAAAACCGGCACCCTGACGCAGGATAAAATTGTGCTGGAGAATCATACCGATATCTCCGGTAAAACCAGCGAACGCGTGCTGCATAGCGCGTGGTTGAACAGTCATTATCAGACCGGACTTAAAAACCTGCTCGATACAGCGGTGCTCGAAGGTACGGATGAAGAGTCAGCGCGCTCGCTGGCCAGTCGTTGGCAA  >  CP009273/4458464‑4458755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`