BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,466,937	A→C	5.0%	T562P (ACT→CCT)	yjgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,466,937	0	A	C	5.0%	881.0 / 30.0	259	T562P (ACT→CCT)	yjgL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (154/90); new base C (8/6); total (162/97)
Fisher's exact test for biased strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

GGGTGAAAAGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGG  >  CP009273/4466789‑4467085

 ggTGAAAAGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAAc                                                                                                                                                     <  2:3424023/149‑1 (MQ=255)

  gTGAAAAGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACt                                                                                                                                                    <  1:2118192/149‑1 (MQ=255)

   tGAAAAGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTa                                                                                                                                                   <  1:3436135/149‑1 (MQ=255)

    gAAAAGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATATAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTAt                                                                                                                                                  <  2:2601472/149‑1 (MQ=255)

     aaaaGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATCAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGTAAAACTGAATAATGAATACAAACTTAGTTTTAGAATGATGCACACTATc                                                                                                                                                 >  1:4641571/1‑149 (MQ=255)

      aaaTATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCt                                                                                                                                                <  2:3038057/149‑1 (MQ=255)

            ttatttTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAAt                                                                                                                                          <  2:415914/149‑1 (MQ=255)

                 ttGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCgaga                                                                                                                                     <  2:1825918/149‑1 (MQ=255)

                 ttGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCgaga                                                                                                                                     <  2:1825408/149‑1 (MQ=255)

                  tGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAACCTATCTTGCAATCGagag                                                                                                                                    >  2:4485761/1‑149 (MQ=255)

                   gATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGt                                                                                                                                   <  1:456540/149‑1 (MQ=255)

                   gATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGt                                                                                                                                   <  2:1169780/149‑1 (MQ=255)

                    aTAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGtt                                                                                                                                  >  1:235283/1‑149 (MQ=255)

                    aTAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGtt                                                                                                                                  >  1:235444/1‑149 (MQ=255)

                    aTAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGtt                                                                                                                                  >  1:1031641/1‑149 (MQ=255)

                       aTTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAAt                                                                                                                               >  1:494371/1‑149 (MQ=255)

                         ttAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGt                                                                                                                             <  1:382866/149‑1 (MQ=255)

                            aaaTATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTAtt                                                                                                                          <  2:2189907/149‑1 (MQ=255)

                              atatGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTAc                                                                                                                        >  1:1781004/1‑149 (MQ=255)

                              atatGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTAc                                                                                                                        >  1:3608909/1‑149 (MQ=255)

                               tatGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTAcc                                                                                                                       >  1:3405450/1‑149 (MQ=255)

                                  gATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCata                                                                                                                    <  2:3326726/149‑1 (MQ=255)

                                  gATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCata                                                                                                                    <  2:3405494/149‑1 (MQ=255)

                                   aTTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCatat                                                                                                                   <  2:3678746/149‑1 (MQ=255)

                                     ttAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCatatat                                                                                                                 <  1:2778711/149‑1 (MQ=255)

                                      tAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATAtt                                                                                                                >  2:1809424/1‑149 (MQ=255)

                                          tgataatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATg                                                                                                            >  2:1153166/1‑149 (MQ=255)

                                          tgataatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATg                                                                                                            <  2:2721308/149‑1 (MQ=255)

                                          tgataatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATg                                                                                                            >  2:4338291/1‑149 (MQ=255)

                                           gataatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGa                                                                                                           >  2:3724555/1‑149 (MQ=255)

                                           gataatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGa                                                                                                           >  1:4347410/1‑149 (MQ=255)

                                           gataatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGa                                                                                                           >  1:1614965/1‑149 (MQ=255)

                                            ataatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGaa                                                                                                          >  1:728411/1‑149 (MQ=255)

                                             taatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCCATCGAGAGTTAATGTATTACCATATATTAATGAAc                                                                                                         >  2:2367665/1‑149 (MQ=255)

                                             taatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAAc                                                                                                         >  2:4561133/1‑149 (MQ=255)

                                             taatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAAc                                                                                                         >  2:449100/1‑149 (MQ=255)

                                              aatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACa                                                                                                        <  2:3669709/149‑1 (MQ=255)

                                               atgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAg                                                                                                       <  2:1665191/149‑1 (MQ=255)

                                                tgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGc                                                                                                      <  1:1107709/149‑1 (MQ=255)

                                                   taaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGtt                                                                                                   >  1:3698658/1‑149 (MQ=255)

                                                    aaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGttt                                                                                                  >  1:2807837/1‑149 (MQ=255)

                                                      ggAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaa                                                                                                >  1:1444564/1‑149 (MQ=255)

                                                       gAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaa                                                                                               >  1:1931412/1‑149 (MQ=255)

                                                        aaTCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaat                                                                                              >  1:478249/1‑149 (MQ=255)

                                                        aaTCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaat                                                                                              <  2:3251130/149‑1 (MQ=255)

                                                         aTCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATTAACAGCGTTtaaata                                                                                             >  2:2911501/1‑149 (MQ=255)

                                                          tCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaataa                                                                                            >  1:668081/1‑149 (MQ=255)

                                                          tCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTtaaataa                                                                                            >  2:3236491/1‑149 (MQ=255)

                                                               taaAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAACCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTaa                                                                                       <  2:4628627/149‑1 (MQ=255)

                                                                    aaaTTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAATTAAACTAAATCCa                                                                                  >  1:4545696/1‑149 (MQ=255)

                                                                       ttttGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCg                                                                               >  2:1366645/1‑149 (MQ=255)

                                                                           gaagaaTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGaaa                                                                           <  1:3119283/149‑1 (MQ=255)

                                                                             agaaTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAAt                                                                         >  2:1380531/1‑149 (MQ=255)

                                                                                aTCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTa                                                                      <  2:3902730/149‑1 (MQ=255)

                                                                                 tCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAACCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTAc                                                                     >  1:4167227/1‑149 (MQ=255)

                                                                                 tCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTAc                                                                     >  1:790646/1‑149 (MQ=255)

                                                                                  cAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACg                                                                    <  2:4678495/149‑1 (MQ=255)

                                                                                   aaCCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGt                                                                   >  2:3512003/1‑149 (MQ=255)

                                                                                    aCCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGta                                                                  <  1:2024137/149‑1 (MQ=255)

                                                                                     ccAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGtat                                                                 >  1:1285458/1‑149 (MQ=255)

                                                                                     ccAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGtat                                                                 <  1:2995328/149‑1 (MQ=255)

                                                                                        gATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGc                                                              >  1:1336292/1‑149 (MQ=255)

                                                                                           aaTGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAAt                                                           >  2:703420/1‑149 (MQ=255)

                                                                                           aaTGATATAAAATAAAAAATGAATTATGAATACAAAATTATATTTTGAATGATGAAATCTATCTTTAAATAGAGAGTTATTTTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATACACCGGAAAATTTACGTATAGCAAt                                                           <  1:2628375/149‑1 (MQ=255)

                                                                                              gTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGa                                                        <  2:173491/149‑1 (MQ=255)

                                                                                               ttATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGaa                                                       >  2:958976/1‑149 (MQ=255)

                                                                                                tATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAac                                                      <  2:4034031/149‑1 (MQ=255)

                                                                                                tATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAac                                                      >  1:982201/1‑149 (MQ=255)

                                                                                                 aTCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAaca                                                     >  2:846332/1‑149 (MQ=255)

                                                                                                    aaaGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACtt                                                  >  1:1499533/1‑149 (MQ=255)

                                                                                                       gAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTgg                                               <  1:1897100/149‑1 (MQ=255)

                                                                                                        aaaaaCTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTggg                                              <  1:613699/149‑1 (MQ=255)

                                                                                                         aaaaCTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCAACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGt                                             >  2:2787504/1‑149 (MQ=255)

                                                                                                           aaCTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTgg                                           >  2:3568992/1‑149 (MQ=255)

                                                                                                             cttaataatGAATACAAACTTAGATTTAGAATGATGCAACCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGgaa                                         <  2:2988919/146‑1 (MQ=255)

                                                                                                                aataatGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGgaagaa                                      <  1:4032382/149‑1 (MQ=255)

                                                                                                                 ataatGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAAt                                     <  1:2954487/149‑1 (MQ=255)

                                                                                                                 ataatGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAAt                                     <  2:1550688/149‑1 (MQ=255)

                                                                                                                      gAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGAcc                                <  2:2488428/149‑1 (MQ=255)

                                                                                                                      gAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGAcc                                >  1:4304319/1‑149 (MQ=255)

                                                                                                                       aaTACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCt                               >  1:1134610/1‑149 (MQ=255)

                                                                                                                        aTACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTa                              >  2:3355390/1‑149 (MQ=255)

                                                                                                                         tACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTAt                             >  2:1179123/1‑149 (MQ=255)

                                                                                                                         tACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTAt                             >  2:762936/1‑149 (MQ=255)

                                                                                                                          aCAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATc                            >  2:140338/1‑149 (MQ=255)

                                                                                                                            aaaCTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCAc                          >  2:3294589/1‑149 (MQ=255)

                                                                                                                            aaaCTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCAc                          <  1:3339800/149‑1 (MQ=255)

                                                                                                                              aCTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTg                        >  2:380166/1‑149 (MQ=255)

                                                                                                                              aCTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTg                        >  1:1986314/1‑149 (MQ=255)

                                                                                                                                  aGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATa                    >  2:4109179/1‑149 (MQ=255)

                                                                                                                                   gATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATaa                   <  1:4468679/149‑1 (MQ=255)

                                                                                                                                    aTTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATaaa                  >  1:3960675/1‑149 (MQ=255)

                                                                                                                                     tttAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCACTAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATaaaa                 >  2:502298/1‑149 (MQ=255)

                                                                                                                                          aaTGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtg            >  1:2456356/1‑149 (MQ=255)

                                                                                                                                          aaTGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAAtg            >  2:2239889/1‑149 (MQ=255)

                                                                                                                                                gCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGc      >  2:2095418/1‑149 (MQ=255)

                                                                                                                                                gCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGc      >  1:1680499/1‑149 (MQ=255)

                                                                                                                                                   aaCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCgg   >  2:3139778/1‑149 (MQ=255)

                                                                                                                                                    aCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCggg  >  1:607885/1‑149 (MQ=255)

                                                                                                                                                    aCTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCggg  >  2:3053400/1‑149 (MQ=255)

GGGTGAAAAGATTTATTTTGATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAGTTAATGTATTACCATATATTAATGAACAGCGTTTAAATAAACTAAATCCACCGGAAAATTTACGTATAGCAATAGAACACTTTGGGTGGAAGAATAGACCTATCACTGCATAAAATAATGTGATGCCGGG  >  CP009273/4466789‑4467085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`