Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,512,693 T→A 80.1% K407* (AAG→TAG)  yjhG ← putative dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,512,6930TA80.1% 705.9 / 175.0 277K407* (AAG→TAG) yjhGputative dehydratase
Reads supporting (aligned to +/- strand):  ref base T (29/26);  new base A (122/99);  total (152/125)
Fisher's exact test for biased strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 277 total aligned reads displayed.

TGGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCA  >  CP009273/4512546‑4512838
                                                                                                                                                   |                                                                                                                                                 
   cGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAAc                                                                                                                                               <  1:1897969/149‑1 (MQ=255)
      tGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGc                                                                                                                                            >  1:2937291/1‑149 (MQ=255)
        gATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTg                                                                                                                                          >  2:3240346/1‑149 (MQ=255)
        gATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTg                                                                                                                                          <  1:2684542/149‑1 (MQ=255)
            tGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGc                                                                                                                                      <  1:1879412/149‑1 (MQ=255)
             gATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGcc                                                                                                                                     <  1:249093/149‑1 (MQ=255)
                cACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGtt                                                                                                                                  <  2:358351/149‑1 (MQ=255)
                 aCCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTc                                                                                                                                 <  2:2866375/149‑1 (MQ=255)
                   cGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCgg                                                                                                                               >  1:2554888/1‑149 (MQ=255)
                    gAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGa                                                                                                                              >  2:12/1‑149 (MQ=255)
                    gAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGa                                                                                                                              >  1:2621485/1‑149 (MQ=255)
                     aaCCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAg                                                                                                                             <  1:3007363/149‑1 (MQ=255)
                     aaCCTTCTGGCGCAATATTGCCCACCGCGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGAATTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCAGACGCCGTTCGGAg                                                                                                                             >  2:491450/1‑149 (MQ=255)
                       ccTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTg                                                                                                                           >  1:765091/1‑149 (MQ=255)
                       ccTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTg                                                                                                                           >  2:2692215/1‑149 (MQ=255)
                        cTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGc                                                                                                                          >  2:609703/1‑149 (MQ=255)
                        cTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGc                                                                                                                          >  2:175668/1‑149 (MQ=255)
                             ggCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCcca                                                                                                                     <  1:1325022/149‑1 (MQ=255)
                                 cAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAg                                                                                                                 <  1:2098075/149‑1 (MQ=255)
                                   atatTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTc                                                                                                               <  2:1921067/149‑1 (MQ=255)
                                    tatTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCg                                                                                                              >  1:1773391/1‑149 (MQ=255)
                                     atTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGa                                                                                                             >  2:2019309/1‑149 (MQ=255)
                                       tGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAgg                                                                                                           <  1:2259724/149‑1 (MQ=255)
                                          ccACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGttt                                                                                                        >  1:1016415/1‑149 (MQ=255)
                                           cACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGtttt                                                                                                       >  2:3203122/1‑149 (MQ=255)
                                              cGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttctt                                                                                                    >  1:4473287/1‑149 (MQ=255)
                                               gggAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttcttt                                                                                                   >  2:2214192/1‑149 (MQ=255)
                                                ggAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttctttc                                                                                                  >  2:1643778/1‑149 (MQ=255)
                                                ggAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttctttc                                                                                                  >  2:2498403/1‑149 (MQ=255)
                                                ggAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttctttc                                                                                                  >  2:3714041/1‑149 (MQ=255)
                                                ggAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttctttc                                                                                                  >  2:157141/1‑149 (MQ=255)
                                                ggAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTtttctttc                                                                                                  >  2:157146/1‑149 (MQ=255)
                                                 gAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCa                                                                                                 <  2:1335680/149‑1 (MQ=255)
                                                   aGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGc                                                                                               <  2:87275/149‑1 (MQ=255)
                                                      tGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtg                                                                                            >  1:621296/1‑149 (MQ=255)
                                                       gATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgc                                                                                           <  1:1643525/149‑1 (MQ=255)
                                                         tAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgctg                                                                                         >  1:912432/1‑149 (MQ=255)
                                                          aGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgctgc                                                                                        >  1:3164443/1‑149 (MQ=255)
                                                          aGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGtgctgc                                                                                        >  1:4560244/1‑149 (MQ=255)
                                                           gTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGcc                                                                                       <  2:408888/149‑1 (MQ=255)
                                                           gTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGcc                                                                                       <  1:3295238/149‑1 (MQ=255)
                                                            ttGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCg                                                                                      >  2:208794/1‑149 (MQ=255)
                                                            ttGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCg                                                                                      >  1:2421295/1‑149 (MQ=255)
                                                             tGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCgg                                                                                     <  1:3138776/149‑1 (MQ=255)
                                                              gAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGGTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGt                                                                                    <  1:1354454/149‑1 (MQ=255)
                                                                 gTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAAc                                                                                 >  2:1350308/1‑149 (MQ=255)
                                                                  ttAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAAcc                                                                                >  2:2372948/1‑149 (MQ=255)
                                                                    aaTCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGt                                                                              >  1:1285706/1‑149 (MQ=255)
                                                                     aTCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTc                                                                             >  1:410500/1‑149 (MQ=255)
                                                                      tCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCa                                                                            >  2:1115948/1‑149 (MQ=255)
                                                                      tCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCa                                                                            <  2:2410617/149‑1 (MQ=255)
                                                                       ccGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCAt                                                                           <  1:2077666/149‑1 (MQ=255)
                                                                              cTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCt                                                                    <  2:2513490/149‑1 (MQ=255)
                                                                               ttttGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCtt                                                                   >  1:200170/1‑149 (MQ=255)
                                                                                tttGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTc                                                                  >  1:2439117/1‑149 (MQ=255)
                                                                                  tgcttgctGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCAt                                                                <  1:692310/149‑1 (MQ=255)
                                                                                  tgcttgctGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCAt                                                                <  1:61012/149‑1 (MQ=255)
                                                                                   gcttgctGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATg                                                               <  2:1291911/149‑1 (MQ=255)
                                                                                    cttgctGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGc                                                              <  2:4502698/149‑1 (MQ=255)
                                                                                    cttgctGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGc                                                              <  1:1115948/149‑1 (MQ=255)
                                                                                      tgctgcGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGcaa                                                            <  2:4012086/149‑1 (MQ=255)
                                                                                          gcGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAAt                                                        >  2:1516446/1‑149 (MQ=255)
                                                                                          gcGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAAt                                                        <  2:4618060/149‑1 (MQ=255)
                                                                                            ggAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATcc                                                      <  1:2873224/149‑1 (MQ=255)
                                                                                              agaCATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGa                                                    <  2:819176/149‑1 (MQ=255)
                                                                                               gaCATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAg                                                   >  1:4619330/1‑149 (MQ=255)
                                                                                                 cATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGc                                                 >  1:3652544/1‑149 (MQ=255)
                                                                                                 cATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGc                                                 <  1:3977879/149‑1 (MQ=255)
                                                                                                   tGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTg                                               >  1:2338371/1‑149 (MQ=255)
                                                                                                    gATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTgc                                              >  1:3805554/1‑149 (MQ=255)
                                                                                                         cTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGa                                         <  2:313470/149‑1 (MQ=255)
                                                                                                          ttCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGAt                                        >  2:3490591/1‑149 (MQ=255)
                                                                                                              tCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCaa                                    >  2:1167988/1‑149 (MQ=255)
                                                                                                              tCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCaa                                    >  1:473810/1‑149 (MQ=255)
                                                                                                                aGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACa                                  >  2:1696417/1‑149 (MQ=255)
                                                                                                                  cGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATg                                <  1:625435/149‑1 (MQ=255)
                                                                                                                   gTTGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGa                               >  1:4605415/1‑149 (MQ=255)
                                                                                                                    ttGATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGAc                              <  2:3299953/149‑1 (MQ=255)
                                                                                                                      gATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACtt                            >  2:787317/1‑149 (MQ=255)
                                                                                                                      gATTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACtt                            >  2:3914894/1‑149 (MQ=255)
                                                                                                                       aTTTGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTc                           >  1:3229257/1‑149 (MQ=255)
                                                                                                                        tttGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTcc                          >  2:225153/1‑149 (MQ=255)
                                                                                                                        tttGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTcc                          <  2:3608872/149‑1 (MQ=255)
                                                                                                                         ttGTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCg                         >  2:4104878/1‑149 (MQ=255)
                                                                                                                           gTTCCTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGc                       >  2:2793551/1‑149 (MQ=255)
                                                                                                                               cTGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTCACCGTCATAACGTCTTCATGCACCAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCacac                   >  1:1398488/1‑149 (MQ=255)
                                                                                                                                tGATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCAcacc                  >  1:2965837/1‑149 (MQ=255)
                                                                                                                                 gATCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCAcacca                 <  1:2269279/149‑1 (MQ=255)
                                                                                                                                  aTCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCAcaccac                >  1:3898622/1‑149 (MQ=255)
                                                                                                                                  aTCGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCAcaccac                >  1:4316879/1‑149 (MQ=255)
                                                                                                                                    cGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCt              <  2:2761601/149‑1 (MQ=255)
                                                                                                                                    cGAGCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCt              <  2:2727473/149‑1 (MQ=255)
                                                                                                                                       gCAGGAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGcc           <  2:1879286/149‑1 (MQ=255)
                                                                                                                                         aGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCAt         >  1:336869/1‑149 (MQ=255)
                                                                                                                                          ggAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATa        >  2:3025596/1‑149 (MQ=255)
                                                                                                                                          ggAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATa        >  2:685597/1‑149 (MQ=255)
                                                                                                                                          ggAGTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATa        >  2:2916584/1‑149 (MQ=255)
                                                                                                                                             gTTGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAg     >  2:3867632/1‑149 (MQ=255)
                                                                                                                                               tGCTAGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGc   <  1:4029113/149‑1 (MQ=255)
                                                                                                                                                gCTTGAACCGCTGACGCCGTTCGGAGAGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCa  <  2:4295451/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGGCGGTGGATTTGATCACCGAACCTTCTGGCGCAATATTGCCCACCGGGAAGGTGATAGTTGAGGTTAATCCGCGCGCTTTTGCTTGCTGCGGAGACATGATCACTTCGTCAGCGTTGATTTGTTCCTGATCGAGCAGGAGTTGCTTGAACCGCTGACGCCGTTCGGAGTGCTCCCACCAGTCGAGGTTTTCTTTCAGCGTGCTGCCGGTAACCGTCATAACGTCTTCATGCAACAATCCGAGGCTGCGCAGATGCAACATGACTTCCGGCACACCACCTGCCATAAAGGCA  >  CP009273/4512546‑4512838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑