Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 360,752 A→G 100% V331A (GTG→GCG)  lacI ← DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273360,7520AG97.6% 46892.8 / inf 11990V331A (GTG→GCG) lacIDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (135/157);  new base G (5621/6073);  total (5758/6232)
Fisher's exact test for biased strand distribution p-value = 5.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 11996 total aligned reads displayed.

CTGATGCTCGGCGAAGCCAAGCCCAGCGCAGATGCGGTCCTCAATCGCGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATA  >  CP009273/360604‑360900
                                                                                                                                                    |                                                                                                                                                    
   gataagctgtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCa                                                                                                                                                   >  2:1999555‑M1/46‑149 (MQ=255)
     taagctgtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAgg                                                                                                                                                 <  2:3084017‑M1/106‑1 (MQ=255)
      aagctgtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAggg                                                                                                                                                <  1:3450400‑M1/107‑1 (MQ=255)
      aagctgtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAggg                                                                                                                                                >  1:993770‑M1/43‑149 (MQ=255)
        gctgtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGggtg                                                                                                                                              >  2:770827‑M1/41‑149 (MQ=255)
           gtcaaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGtt                                                                                                                                           <  1:2090501‑M1/112‑1 (MQ=255)
             caaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGtttt                                                                                                                                         <  1:3994489‑M1/114‑1 (MQ=255)
              aaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGttttt                                                                                                                                        <  1:2174252‑M1/115‑1 (MQ=255)
              aaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGttttt                                                                                                                                        >  1:2843380‑M1/35‑149 (MQ=255)
              aaaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGttttt                                                                                                                                        >  2:2660676‑M1/35‑149 (MQ=255)
               aaccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttc                                                                                                                                       <  1:3542952‑M1/116‑1 (MQ=255)
                accagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttct                                                                                                                                      >  1:3371391‑M1/33‑149 (MQ=255)
                accagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttct                                                                                                                                      <  1:4589062‑M1/117‑1 (MQ=255)
                 ccagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttctt                                                                                                                                     <  2:4578746‑M1/118‑1 (MQ=255)
                  cagatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttcttt                                                                                                                                    <  1:3726044‑M1/119‑1 (MQ=255)
                   agatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttctttt                                                                                                                                   <  1:1942323‑M1/120‑1 (MQ=255)
                   agatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttctttt                                                                                                                                   >  1:4488994‑M1/30‑149 (MQ=255)
                   agatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttctttt                                                                                                                                   >  1:3624997‑M1/30‑149 (MQ=255)
                   agatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttctttt                                                                                                                                   >  1:1333493‑M1/30‑149 (MQ=255)
                   agaacaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttctttt                                                                                                                                   <  1:2439683‑M1/120‑1 (MQ=255)
                    gatcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTttttcttttc                                                                                                                                  <  2:3668451‑M1/121‑1 (MQ=255)
                     atcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTCTTGGGCGCCAGGGTGGTTTTTCTTTTCa                                                                                                                                 <  2:1682162‑M1/122‑1 (MQ=255)
                     atcaattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCa                                                                                                                                 >  1:2362342‑M1/28‑149 (MQ=255)
                       caattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCAcc                                                                                                                               >  2:1915019‑M1/26‑149 (MQ=255)
                        aattcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCa                                                                                                                              <  1:726758‑M1/125‑1 (MQ=255)
                          ttcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGt                                                                                                                            >  1:1000469‑M1/23‑149 (MQ=255)
                           tcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTg                                                                                                                           >  2:3148181‑M1/22‑149 (MQ=255)
                           tcgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTg                                                                                                                           >  1:46809‑M1/22‑149 (MQ=255)
                            cgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTga                                                                                                                          >  2:3580945‑M1/21‑149 (MQ=255)
                            cgcgctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTga                                                                                                                          >  2:4485801‑M1/21‑149 (MQ=255)
                              cgctaacttacattaagtGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTgaga                                                                                                                        >  1:2935836‑M1/19‑149 (MQ=255)
                               gctaacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGAc                                                                                                                       >  2:2159059‑M1/18‑149 (MQ=255)
                                 taacttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTATTTTAACCAGTGAGACgg                                                                                                                     <  1:1585872‑M1/134‑1 (MQ=255)
                                    cttacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCa                                                                                                                  <  2:1081037‑M1/137‑1 (MQ=255)
                                      tacattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAAc                                                                                                                >  1:816137‑M1/11‑149 (MQ=255)
                                       acattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACa                                                                                                               <  1:2662583‑M1/140‑1 (MQ=255)
                                       acattaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACa                                                                                                               <  2:3397640‑M1/140‑1 (MQ=255)
                                        cattaagtGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAg                                                                                                              >  1:2586029‑M1/9‑149 (MQ=255)
                                          ttaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGGGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCt                                                                                                            <  1:2512626‑M1/143‑1 (MQ=255)
                                          ttaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCt                                                                                                            >  1:3105500‑M1/7‑149 (MQ=255)
                                          ttaattGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCt                                                                                                            >  2:1593195‑M1/7‑149 (MQ=255)
                                              ttGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGAtt                                                                                                        <  2:4099162‑M1/147‑1 (MQ=255)
                                                 cgTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGcc                                                                                                     >  1:1166633/1‑149 (MQ=255)
                                                      cgcTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCa                                                                                                <  2:1167283/149‑1 (MQ=255)
                                                        cTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATAGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTAATTTAACCAGAGAGACGGGCAACAGCTGATTTACCTTAAcc                                                                                              >  1:4398199/1‑149 (MQ=255)
                                                          cACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGc                                                                                            <  1:91615/149‑1 (MQ=255)
                                                             tGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTg                                                                                         <  2:922326/149‑1 (MQ=255)
                                                             tGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGAATTGGGCGCCAGGGTGGTTTTTGTTTTAACCAGTGAGACAGGCCAAAGCTGATTGACCATCACCGccag                                                                                         >  1:1420294/1‑147 (MQ=255)
                                                                 cGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGccc                                                                                     >  2:2484621/1‑149 (MQ=255)
                                                                    tttCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTga                                                                                  >  2:3234186/1‑149 (MQ=255)
                                                                    tttCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTga                                                                                  <  1:417462/149‑1 (MQ=255)
                                                                      tCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTgaga                                                                                >  1:1092065/1‑149 (MQ=255)
                                                                             gggAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTGTTTTGACCAGTGAGACGGGCCACACCAGATTGCCCTTAACCCCCGGGCCCGGAGAGAGTTgc                                                                         >  1:3912390/1‑149 (MQ=255)
                                                                                 aaCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTgcagca                                                                     >  2:4660552/1‑149 (MQ=255)
                                                                                  aCCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCaa                                                                    <  1:1860482/149‑1 (MQ=255)
                                                                                    cTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTAACCAGTGAGACGGGCCACACCAGATTTCCCCTAAACCCCCGGCCCAGAGAGAGTTTCAGCaaac                                                                  >  1:2744771/1‑147 (MQ=255)
                                                                                     tGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCg                                                                 <  2:1840878/149‑1 (MQ=255)
                                                                                     tGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTGTTTATTTTAACCACTGAGAAGGGCCACAGCAGATTTCCCATCACCCCCCGGCCCCGAGAGAGTTGCAGCAACCg                                                                 >  1:425585/1‑149 (MQ=255)
                                                                                      gTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCgg                                                                <  2:3314914/149‑1 (MQ=255)
                                                                                       tCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTTAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGt                                                               <  2:692556/149‑1 (MQ=255)
                                                                                          tGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCa                                                            <  2:4259014/149‑1 (MQ=255)
                                                                                             cAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGc                                                         <  2:3755856/149‑1 (MQ=255)
                                                                                                  gCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGtt                                                    <  2:2084318/149‑1 (MQ=255)
                                                                                                     ttAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGc                                                 <  1:3021482/149‑1 (MQ=255)
                                                                                                     ttAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTATTTTCACCAGTGAGAAGGGCAACAGCTGATTTCCCTTCACCGCCAGGCCCGGAGATAGTTGCAGCAAGCGGTCCACGCTGGTTTGc                                                 >  1:63637/1‑149 (MQ=255)
                                                                                                       aaTGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGccc                                               >  1:1761056/1‑149 (MQ=255)
                                                                                                        aTGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGcccc                                              >  1:3291793/1‑149 (MQ=255)
                                                                                                        aTGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGcccc                                              <  1:4081687/149‑1 (MQ=255)
                                                                                                        aTGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGcccc                                              <  2:2740076/149‑1 (MQ=255)
                                                                                                         tGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCca                                             >  2:134325/1‑149 (MQ=255)
                                                                                                         tGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCca                                             <  2:2922490/149‑1 (MQ=255)
                                                                                                           aaTCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCcagc                                           >  2:2404435/1‑149 (MQ=255)
                                                                                                             tCGGCCACCGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCcagcag                                         <  2:3003064/149‑1 (MQ=255)
                                                                                                             tCGGCCAACTCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCcagcag                                         <  2:283289/149‑1 (MQ=255)
                                                                                                                   aaCGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGaaa                                   >  2:3840516/1‑149 (MQ=255)
                                                                                                                    aCGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTAACCAGTGAGACGGGCAACAGCTGATTTCCCATCACCCCCTGGCCCTGCGAGAGTTTCAGCAAGCGGTCCAAGCTGGGTTTCCCCAGCAGGCGaaaa                                  >  1:539314/1‑149 (MQ=255)
                                                                                                                     cgcgcgGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAAt                                 <  2:538508/149‑1 (MQ=255)
                                                                                                                          ggggAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGt                            <  2:3489091/149‑1 (MQ=255)
                                                                                                                           gggAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATAGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGtt                           >  2:2328160/1‑149 (MQ=255)
                                                                                                                            tgagtGGCGGTTTGCGTATTGGGCGCCAGGTTGGTTTTTCTTTTCACCAGTGAGTCGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCATGttt                          <  1:4322728/148‑1 (MQ=255)
                                                                                                                            ggAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTTCCCCAGCAGGCGAAAATCCTGttt                          >  2:4341897/1‑149 (MQ=255)
                                                                                                                            ggAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGttt                          >  2:614247/1‑149 (MQ=255)
                                                                                                                            ggAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGttt                          <  2:3935610/149‑1 (MQ=255)
                                                                                                                              agagGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGa                        <  1:3195604/149‑1 (MQ=255)
                                                                                                                              agagGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCATCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGa                        >  2:1750188/1‑149 (MQ=255)
                                                                                                                               gagGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAt                       >  2:2572147/1‑149 (MQ=255)
                                                                                                                                 ggCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtgg                     <  2:4013562/149‑1 (MQ=255)
                                                                                                                                 ggCGGTGTGCGTTTTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCATGTGGTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtgg                     <  2:2238795/149‑1 (MQ=255)
                                                                                                                                  gCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGAtggt                    >  1:1497373/1‑149 (MQ=255)
                                                                                                                                     gTTTGCGTATTGTGCAACAGGGTTGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTTATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCATCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATggtggt                 <  1:1240688/149‑1 (MQ=255)
                                                                                                                                     gTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATggtggt                 <  2:1499017/149‑1 (MQ=255)
                                                                                                                                     gTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCAACAGTGAGACGGGCAACAGCTGATTGCCCTTAACCGCCAGGCCCTGAGAGAGTTTCAGCAAGCGGTACACCCAGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATtgtggt                 >  1:2277914/1‑149 (MQ=255)
                                                                                                                                      tttGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGtt                <  2:4182817/149‑1 (MQ=255)
                                                                                                                                      tttGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGtt                <  2:841687/149‑1 (MQ=255)
                                                                                                                                        tGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTaa              <  2:1424625/149‑1 (MQ=255)
                                                                                                                                        tGCGTATTGGGCGCCAGGGTGGTTTGTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCGTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTaa              <  2:1256579/149‑1 (MQ=255)
                                                                                                                                           gTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAAcgg           <  1:3811406/149‑1 (MQ=255)
                                                                                                                                             aTTGGGCGCCAGGGTGGTTTTTATTTTCACCACTGAGACGGGCAACAGCTGATTGCCCATCACCCCCTGGCCCAGAGAGAGTTGCAGCAAGCGGTCCACCCAGGTTTGCCCCCGCAGGCGAAAAACCAGTTTGATGGTGGTTAAcggcg         >  1:168815/1‑149 (MQ=255)
                                                                                                                                                 ggCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCgggct     >  2:4402695/1‑147 (MQ=255)
                                                                                                                                                  gCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGata    <  1:2029194/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGATGCTCGGCGAAGCCAAGCCCAGCGCAGATGCGGTCCTCAATCGCGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCACCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATA  >  CP009273/360604‑360900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑