Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 406,075 C→A 86.1% P159Q (CCG→CAG)  mak → manno(fructo)kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273406,0750CA86.1% 798.2 / 131.6 295P159Q (CCG→CAG) makmanno(fructo)kinase
Reads supporting (aligned to +/- strand):  ref base C (18/23);  new base A (115/139);  total (133/162)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 297 total aligned reads displayed.

GCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGT  >  CP009273/405927‑406223
                                                                                                                                                    |                                                                                                                                                    
gCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATcc                                                                                                                                                      <  2:625817/149‑1 (MQ=255)
  ggTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGc                                                                                                                                                    <  2:2372286/149‑1 (MQ=255)
   gTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCt                                                                                                                                                   >  2:1157197/1‑149 (MQ=255)
         gAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGtg                                                                                                                                             >  2:4449429/1‑149 (MQ=255)
          aaGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGtgg                                                                                                                                            >  2:2963008/1‑149 (MQ=255)
           aGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGtgga                                                                                                                                           >  1:3439209/1‑149 (MQ=255)
            gCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGtggat                                                                                                                                          <  1:1056928/149‑1 (MQ=255)
             cAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGtggatg                                                                                                                                         <  1:2919858/149‑1 (MQ=255)
               gTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGtggatgga                                                                                                                                       >  1:1252556/1‑149 (MQ=255)
                   aTGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGgacgaa                                                                                                                                   <  1:550237/149‑1 (MQ=255)
                    tGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGgacgaag                                                                                                                                  >  2:3284566/1‑149 (MQ=255)
                            cAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTg                                                                                                                          <  1:1876472/149‑1 (MQ=255)
                            cAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTg                                                                                                                          >  1:1211712/1‑149 (MQ=255)
                             aGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGc                                                                                                                         >  2:1944913/1‑149 (MQ=255)
                                 ggAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTa                                                                                                                     >  1:1102419/1‑149 (MQ=255)
                                 ggAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTa                                                                                                                     <  1:2427031/149‑1 (MQ=255)
                                 ggAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTa                                                                                                                     <  2:2617814/149‑1 (MQ=255)
                                  gAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTAt                                                                                                                    >  2:4528639/1‑149 (MQ=255)
                                   aGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATc                                                                                                                   >  2:532638/1‑149 (MQ=255)
                                    gcgcAGACGGTATTTGCCGTGCTTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATcg                                                                                                                  >  2:3932059/1‑149 (MQ=255)
                                       cAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGa                                                                                                               <  1:1612721/149‑1 (MQ=255)
                                        aGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAg                                                                                                              >  1:1973190/1‑149 (MQ=255)
                                        aGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAg                                                                                                              >  2:1853349/1‑149 (MQ=255)
                                        aGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAg                                                                                                              <  2:1221859/149‑1 (MQ=255)
                                        aGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAg                                                                                                              <  1:40971/149‑1 (MQ=255)
                                        aGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAg                                                                                                              >  2:3840508/1‑149 (MQ=255)
                                            ggTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAg                                                                                                          <  1:4591683/149‑1 (MQ=255)
                                                 ttGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCt                                                                                                     <  2:3636837/149‑1 (MQ=255)
                                                  tGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCtt                                                                                                    <  1:1745237/149‑1 (MQ=255)
                                                  tGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCtt                                                                                                    <  2:1810699/149‑1 (MQ=255)
                                                    ccGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGt                                                                                                  >  1:453521/1‑149 (MQ=255)
                                                    ccGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGt                                                                                                  <  2:1726636/149‑1 (MQ=255)
                                                    ccGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGt                                                                                                  <  2:2595126/149‑1 (MQ=255)
                                                      gTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTa                                                                                                >  1:4168270/1‑149 (MQ=255)
                                                      gTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGtgg                                                                                                >  2:4358924/1‑147 (MQ=255)
                                                      gTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTa                                                                                                >  1:779265/1‑149 (MQ=255)
                                                        gATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTAtt                                                                                              <  1:2280461/149‑1 (MQ=255)
                                                            aTCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCgg                                                                                          >  2:1305963/1‑149 (MQ=255)
                                                            aTCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCgg                                                                                          >  1:1347155/1‑149 (MQ=255)
                                                            aTCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCgg                                                                                          <  2:420570/149‑1 (MQ=255)
                                                             tCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGt                                                                                         <  2:3042096/149‑1 (MQ=255)
                                                              cGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTa                                                                                        <  1:666283/149‑1 (MQ=255)
                                                              cGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTa                                                                                        <  1:3932904/149‑1 (MQ=255)
                                                                gCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTaaa                                                                                      <  2:1520515/149‑1 (MQ=255)
                                                                  aCGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACa                                                                                    >  1:4255363/1‑149 (MQ=255)
                                                                   cGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACaa                                                                                   >  2:242210/1‑149 (MQ=255)
                                                                    gggATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAg                                                                                  <  1:473496/149‑1 (MQ=255)
                                                                           ggcgCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTAt                                                                           <  1:4428765/149‑1 (MQ=255)
                                                                           ggcgCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTAt                                                                           <  2:45169/149‑1 (MQ=255)
                                                                            gcgcGGGCGTGGCATTCAATGGGCGGTCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTAtt                                                                          <  1:3630765/149‑1 (MQ=255)
                                                                                 ggCGTGACATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAAc                                                                     >  1:936635/1‑149 (MQ=255)
                                                                                    gTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCtt                                                                  <  2:2059574/149‑1 (MQ=255)
                                                                                    gTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCtt                                                                  <  2:2115218/149‑1 (MQ=255)
                                                                                    gTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCtt                                                                  <  1:2344054/149‑1 (MQ=255)
                                                                                     tGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCttt                                                                 <  1:2221116/149‑1 (MQ=255)
                                                                                       gCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTAt                                                               <  1:2068928/149‑1 (MQ=255)
                                                                                       gCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTAt                                                               >  2:2169567/1‑149 (MQ=255)
                                                                                       gCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTAt                                                               <  1:464193/149‑1 (MQ=255)
                                                                                        cATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTAtt                                                              <  1:521035/149‑1 (MQ=255)
                                                                                          ttCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTc                                                            >  1:273560/1‑149 (MQ=255)
                                                                                              aTGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGc                                                        <  1:352897/149‑1 (MQ=255)
                                                                                              aTGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGc                                                        >  2:818/1‑149 (MQ=255)
                                                                                               tGGGCGGGCGCATATCGGCGGCTATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCa                                                       <  2:941252/149‑1 (MQ=255)
                                                                                               tGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCa                                                       >  2:3460509/1‑149 (MQ=255)
                                                                                               tGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCa                                                       >  2:3848673/1‑149 (MQ=255)
                                                                                                gggcgggcGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCAc                                                      >  1:3978888/1‑149 (MQ=255)
                                                                                                gggcgggcGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCAc                                                      >  1:102504/1‑149 (MQ=255)
                                                                                                   cgggcgCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACggg                                                   <  1:3166733/149‑1 (MQ=255)
                                                                                                     ggcgCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGAt                                                 <  2:1426909/149‑1 (MQ=255)
                                                                                                     ggcgCATATCGGCGGCAAGGGCACGGCAGGTGAGTGGGGACACAATCAGCGACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGAt                                                 <  2:2818526/149‑1 (MQ=255)
                                                                                                      gcgcATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGAtt                                                <  2:1397360/149‑1 (MQ=255)
                                                                                                      gcgcATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGAtt                                                <  1:2427049/149‑1 (MQ=255)
                                                                                                      gcgcATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGAtt                                                <  2:1765888/149‑1 (MQ=255)
                                                                                                        gcATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTcg                                              >  1:1553345/1‑149 (MQ=255)
                                                                                                        gcATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTcg                                              <  1:3280779/149‑1 (MQ=255)
                                                                                                           tatCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGa                                           <  2:3486843/149‑1 (MQ=255)
                                                                                                            atCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGAt                                          <  1:606457/149‑1 (MQ=255)
                                                                                                            atCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGAt                                          <  2:2835374/149‑1 (MQ=255)
                                                                                                            atCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGAt                                          <  2:4015696/149‑1 (MQ=255)
                                                                                                                gcggcAATGGCACGGCAGGGGAGTGGGGACACAATCAGCTACCGTGGATGGCCGAAGACGAACTGCGTTATCGCGAGGACGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGCTGGAt                                      >  2:3529642/1‑149 (MQ=255)
                                                                                                                 cggcAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGAtt                                     <  1:1181071/149‑1 (MQ=255)
                                                                                                                  ggcAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTa                                    >  1:349103/1‑149 (MQ=255)
                                                                                                                   gcAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTAt                                   >  2:2971001/1‑149 (MQ=255)
                                                                                                                   gcAATGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTAt                                   >  1:319809/1‑149 (MQ=255)
                                                                                                                     aaTGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTAtcg                                 >  1:1398257/1‑149 (MQ=255)
                                                                                                                      aTGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTAtcgt                                >  1:3346882/1‑149 (MQ=255)
                                                                                                                       tGGCACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTAtcgtc                               <  2:4027669/149‑1 (MQ=255)
                                                                                                                          cACGGCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGtt                            <  1:2667639/149‑1 (MQ=255)
                                                                                                                              gCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAg                        >  1:3059637/1‑149 (MQ=255)
                                                                                                                              gCAGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGCAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAg                        <  1:7052/149‑1 (MQ=255)
                                                                                                                                aGGTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCg                      <  1:2877916/149‑1 (MQ=255)
                                                                                                                                  gTGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGa                    <  2:1252556/149‑1 (MQ=255)
                                                                                                                                   tGAGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAATGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGAc                   <  2:1728227/149‑1 (MQ=255)
                                                                                                                                     aGTGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGTGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACAt                 >  2:4107955/1‑149 (MQ=255)
                                                                                                                                       tGGGGACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATgc               >  2:3093649/1‑149 (MQ=255)
                                                                                                                                          ggACACAATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCACTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTTAGCGGACAATCGCt            >  1:4368045/1‑149 (MQ=255)
                                                                                                                                             cacaATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGaa         >  1:2478865/1‑149 (MQ=255)
                                                                                                                                             cacaATCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGaa         >  1:4268377/1‑149 (MQ=255)
                                                                                                                                                aaTCAGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAgg      >  1:3656758/1‑149 (MQ=255)
                                                                                                                                                    cGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGt  >  1:277228/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGT  >  CP009273/405927‑406223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑