BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	418,958	A→T	6.4%	N329Y (AAT→TAT)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	418,958	0	A	T	6.4%	1067.0 / 47.4	296	N329Y (AAT→TAT)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand): ref base A (131/146); new base T (9/10); total (140/156)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 299 total aligned reads displayed.

TGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATG  >  CP009273/418811‑419105

 ggTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGACAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCTCGGGGAAGACAGTATTGTCCGCCACTGTCTGAAAGCGCCGTGGAat                                                                                                                                                   >  2:1005120/1‑149 (MQ=255)

  gTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGtata                                                                                                                                                  <  2:3043058/149‑1 (MQ=255)

   tACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAatat                                                                                                                                                 >  2:3244855/1‑149 (MQ=255)

   tACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAatat                                                                                                                                                 <  2:3837618/149‑1 (MQ=255)

    aCTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATg                                                                                                                                                >  2:85627/1‑149 (MQ=255)

     cTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATgg                                                                                                                                               >  1:748435/1‑149 (MQ=255)

      tCGTTTAGTGACGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGa                                                                                                                                              >  1:1640077/1‑149 (MQ=255)

       cGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAc                                                                                                                                             <  1:3520713/149‑1 (MQ=255)

           tAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAcggct                                                                                                                                         <  2:4437454/149‑1 (MQ=255)

           tAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAcggct                                                                                                                                         <  2:2879648/149‑1 (MQ=255)

            aGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGAGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAcggctg                                                                                                                                        >  1:2431637/1‑149 (MQ=255)

             gTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAcggctgg                                                                                                                                       <  1:4544710/149‑1 (MQ=255)

             gTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAcggctgg                                                                                                                                       >  1:865949/1‑149 (MQ=255)

              tgatgaTGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAcggctggc                                                                                                                                      <  1:1262999/149‑1 (MQ=255)

                   atgGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAcggctggcggctg                                                                                                                                 <  1:4293963/149‑1 (MQ=255)

                   atgGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGAcggctggcggctg                                                                                                                                 <  1:285807/149‑1 (MQ=255)

                     ggCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGa                                                                                                                               <  2:1354821/149‑1 (MQ=255)

                     ggCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGa                                                                                                                               >  2:3469998/1‑149 (MQ=255)

                        aCGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGTATATGGACGGCTGGCGGCTGGAtgt                                                                                                                            >  2:3691741/1‑149 (MQ=255)

                        aCGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGAtgt                                                                                                                            >  2:2433525/1‑149 (MQ=255)

                               tCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCat                                                                                                                     >  1:4092186/1‑149 (MQ=255)

                                cGACTGGCTTGTCTATGCCAGCTTGCCGAAGATGGATTATCAGTCGGAAAGTCTGTTGAATGAAATTTATCGCGGGGAAGACAGTATGGTCCGCCAATGGCTGAAAGCGCCGTGGAATATTGACGGCTGGCGGCTGGATGTGGTGCata                                                                                                                    <  1:2024041/149‑1 (MQ=255)

                                cGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCata                                                                                                                    <  1:524819/149‑1 (MQ=255)

                                 gACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGTATATGGACGGCTGGCGGCTGGATGTGGTGCatat                                                                                                                   <  2:1773392/149‑1 (MQ=255)

                                 gACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGTATATGGACGGCTGGCGGCTGGATGTGGTGCatat                                                                                                                   <  2:1032214/149‑1 (MQ=255)

                                   cTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGc                                                                                                                 >  1:2906273/1‑149 (MQ=255)

                                   cTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGc                                                                                                                 >  1:4231277/1‑149 (MQ=255)

                                    tggcttggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAACGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCt                                                                                                                <  1:4666740/149‑1 (MQ=255)

                                        ttggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGCTGTGGTGCATATGCTgggg                                                                                                            >  1:2778692/1‑149 (MQ=255)

                                        ttggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTgggg                                                                                                            >  1:3391668/1‑149 (MQ=255)

                                        ttggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTgggg                                                                                                            <  2:1061591/149‑1 (MQ=255)

                                         tgtctATGCCAGCGTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGTTGCATATGCTggggg                                                                                                           <  2:4547184/146‑1 (MQ=255)

                                          ggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGa                                                                                                          >  1:1960076/1‑149 (MQ=255)

                                              aTGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCg                                                                                                      >  1:1000265/1‑149 (MQ=255)

                                               tGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCgg                                                                                                     >  2:472509/1‑149 (MQ=255)

                                                   ggCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTgg                                                                                                 >  2:2552825/2‑149 (MQ=255)

                                                   ggCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTgg                                                                                                 >  2:2552150/2‑149 (MQ=255)

                                                   aGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTgg                                                                                                 <  1:3465299/149‑1 (MQ=255)

                                                           gAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCa                                                                                         >  2:3565005/1‑149 (MQ=255)

                                                             aGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCaat                                                                                       >  1:3142636/1‑149 (MQ=255)

                                                             aGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCaat                                                                                       >  1:235013/1‑149 (MQ=255)

                                                                 ggATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAataata                                                                                   >  1:3688618/1‑149 (MQ=255)

                                                                 ggATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAataata                                                                                   >  2:1695494/1‑149 (MQ=255)

                                                                  gATTATCAGTCGGAAAGTGTGGTGAATTAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAACGCGCCGTGTAATATGGACGGCTGGCGGCTGGTTGTGGTGAATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAatat                                                                                  <  1:2995092/149‑1 (MQ=255)

                                                                   aTTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGTATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATg                                                                                 >  2:2385271/1‑149 (MQ=255)

                                                                   aTTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGTATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATg                                                                                 >  2:3761897/1‑149 (MQ=255)

                                                                      aTCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGTGGCGCGCAATAATATgcag                                                                              >  1:1417344/1‑149 (MQ=255)

                                                                      aTCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATgcag                                                                              >  2:1293480/1‑149 (MQ=255)

                                                                       tCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACGGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGTTGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATgcagc                                                                             <  2:2685465/149‑1 (MQ=255)

                                                                        cattCGGAATGTCTGGTTAATTAATTTTATGCCGGGGAAGACATTATTGTCCGCAAGTGGCTTAAAGCGCCGTTTAATATGGACGGCTGGCGTATGGATTTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATgcagca                                                                            <  1:887357/146‑1 (MQ=255)

                                                                          gTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACg                                                                          >  2:1143764/1‑149 (MQ=255)

                                                                          gTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACg                                                                          >  2:675781/1‑149 (MQ=255)

                                                                            cGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGtt                                                                        >  2:1572735/1‑149 (MQ=255)

                                                                             ggAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTg                                                                       >  1:2579949/1‑149 (MQ=255)

                                                                             ggAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTg                                                                       >  2:2938154/1‑149 (MQ=255)

                                                                              taaaGTCTGGTGAATGAAATTTATCGCGGGGAAGACTGTATTGTCCGCCACTGGCTTAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATTTGCTGGGGGAGGTGGGTGGGGCGCGCAATAATATGCAGCACGTTGc                                                                      <  1:3331312/148‑1 (MQ=255)

                                                                              gAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGc                                                                      <  2:116399/149‑1 (MQ=255)

                                                                               aaaGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGcc                                                                     <  2:552165/149‑1 (MQ=255)

                                                                                aaGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACGGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCg                                                                    <  2:505120/149‑1 (MQ=255)

                                                                                    cTGGTTAATGAATTTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATTTGGACGGCTGGCGGCTGGTTGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGAt                                                                <  1:3496178/149‑1 (MQ=255)

                                                                                     tGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCAGTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATc                                                               <  1:693059/149‑1 (MQ=255)

                                                                                       gTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCAc                                                             <  2:3581674/149‑1 (MQ=255)

                                                                                        tgaatgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACGGGCTGAAAGCGCCGTGGATTCTGGACGGCGGGCGGCGGGAGGTGGGGCATATGCTGGGGGCGGCGGGTGGGGCGCGCAATAAGATGCAGCACGTTGCCGGGATCAcc                                                            <  2:2632650/149‑1 (MQ=255)

                                                                                          aatgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGa                                                          >  1:173899/1‑149 (MQ=255)

                                                                                          aatgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGa                                                          >  1:2252386/1‑149 (MQ=255)

                                                                                            tgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAg                                                        >  2:3363680/1‑149 (MQ=255)

                                                                                              aattttATCGCGGGGAAGACATTATTGTCCGCCAATGGCTGAAAGCGCCTTGTATTATGGCCGGCTGGCGGGTGGTTGTGGTGCATATGCTGGGGTAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcg                                                      <  1:3181975/146‑1 (MQ=255)

                                                                                               aaTTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATTTGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcgg                                                     <  1:1455846/149‑1 (MQ=255)

                                                                                                aTTTATCGCGGGGAAGACAGTATTGGCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcggc                                                    <  2:4342228/149‑1 (MQ=255)

                                                                                                 tttATCGCGGGGAAGACTGTTTGGTCCGCCACTGGCTGAACGCGCCGTGTAATATGGACGGCTGGGGGCTGGATGTGGTGCATTTGCGGGGGGCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcggcg                                                   <  1:1199324/149‑1 (MQ=255)

                                                                                                 tttATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcggcg                                                   >  2:4196490/1‑149 (MQ=255)

                                                                                                 tttATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcggcg                                                   <  1:598524/149‑1 (MQ=255)

                                                                                                   tATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaa                                                 >  2:614313/1‑149 (MQ=255)

                                                                                                     tCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaaag                                               >  2:3499150/1‑149 (MQ=255)

                                                                                                          gggAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAAcc                                          >  2:4627112/1‑149 (MQ=255)

                                                                                                            gAAGACTGTATGGTCGGCCACGGGCTGAACGGGCCGTGGAATTTGGCGGGGGGGCGGCGGGAGGGGGGGCATAGGGGGGGGGAGGCGGGGGGGGCGCGCAAGAAGATGCAGCACGTTGCCGGGATCACCGACGCGGCGAAAGAAACCCa                                        <  2:137252/149‑1 (MQ=255)

                                                                                                            gAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATTTGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCa                                        <  2:3979488/149‑1 (MQ=255)

                                                                                                             aaGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAg                                       >  1:1570117/1‑149 (MQ=255)

                                                                                                             aaGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAg                                       >  1:2385375/1‑149 (MQ=255)

                                                                                                                 cAGTTTGGTCCGCCACGGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGAGGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCgg                                   <  1:4464765/149‑1 (MQ=255)

                                                                                                                     aTTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGc                               >  2:2023281/1‑149 (MQ=255)

                                                                                                                        gTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGta                            <  1:1064546/149‑1 (MQ=255)

                                                                                                                         tCCGCCACTGGCTGAAAGCGCCGTGGAATTTGGACGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGtat                           <  1:4350513/149‑1 (MQ=255)

                                                                                                                           ggcaaCTGGCTGAACGCGCCGTGGAATTTGGACGGCTGGCGGCTGTATGTGGTGCATATGGTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTatat                         <  2:3121795/145‑1 (MQ=255)

                                                                                                                           cGCCACTGGCTGAAAGCGCCGTGGTATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTatat                         <  2:2261334/149‑1 (MQ=255)

                                                                                                                             ccACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTg                       >  1:3570547/1‑149 (MQ=255)

                                                                                                                              cACTGGCTGAAAGCGCCGTGGATTATGGACGGCTGGCGGCTGGATGTTGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGt                      <  1:294607/149‑1 (MQ=255)

                                                                                                                               aCTGGCTGAACGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTc                     <  2:1501095/149‑1 (MQ=255)

                                                                                                                               aCTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTc                     >  2:689835/1‑149 (MQ=255)

                                                                                                                               aCTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGAGGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTc                     <  2:1643650/149‑1 (MQ=255)

                                                                                                                                  ggCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGc                  >  2:210687/1‑149 (MQ=255)

                                                                                                                                  ggCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGc                  >  2:3308173/1‑149 (MQ=255)

                                                                                                                                       aaaGCGCCGTGGTATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACa             >  1:395534/1‑149 (MQ=255)

                                                                                                                                       aaaGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACa             >  1:4406406/1‑149 (MQ=255)

                                                                                                                                         aGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAtt           >  1:3832851/1‑149 (MQ=255)

                                                                                                                                           cgcCGTGTAATTTGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAtttt         <  1:215721/149‑1 (MQ=255)

                                                                                                                                           cgcCGTGGAATAGGGACGGCGGGCGGCTGGATGTGGGGCATAGGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAtttt         <  2:2150036/149‑1 (MQ=255)

                                                                                                                                            gcCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATAGGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTg        <  2:1886829/149‑1 (MQ=255)

                                                                                                                                                tGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGa    >  2:3905430/1‑149 (MQ=255)

                                                                                                                                                  gAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATg  >  2:2557541/1‑149 (MQ=255)

TGGTACTCGTTTAGTGATGATGGCACGGCGCTCGACTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATG  >  CP009273/418811‑419105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`