Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 419,663 G→T 38.5% E564* (GAA→TAA)  malZ → maltodextrin glucosidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273419,6630GT38.5% 368.9 / inf 290E564* (GAA→TAA) malZmaltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base G (67/111);  new base T (46/66);  total (113/177)
Fisher's exact test for biased strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

AGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGAC  >  CP009273/419517‑419811
                                                                                                                                                  |                                                                                                                                                    
aGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGaa                                                                                                                                                    >  1:4164300/1‑149 (MQ=255)
  cGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAgt                                                                                                                                                  >  2:944879/1‑149 (MQ=255)
  cGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAgt                                                                                                                                                  <  1:167866/149‑1 (MQ=255)
   gAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAgtg                                                                                                                                                 <  1:1068671/149‑1 (MQ=255)
   gAATGAGTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAgtg                                                                                                                                                 <  1:13684/149‑1 (MQ=255)
    aaTGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAgtgg                                                                                                                                                <  2:4568815/149‑1 (MQ=255)
     aagATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAATATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAgtggt                                                                                                                                               <  1:1078690/147‑1 (MQ=255)
        aTTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCt                                                                                                                                            <  2:4666499/149‑1 (MQ=255)
          tGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTAc                                                                                                                                          <  1:1600674/149‑1 (MQ=255)
            cgcTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTAccc                                                                                                                                        <  2:1934911/149‑1 (MQ=255)
            cgcTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTAccc                                                                                                                                        <  1:3653675/149‑1 (MQ=255)
                gCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGt                                                                                                                                    <  2:3807200/149‑1 (MQ=255)
                  gTAATAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGATTTATGAGTAAGATAACTTGGTGTTATTTGTCCGCGTGCTGAATCAGCAACGTGTAATGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACACTCGTCa                                                                                                                                  >  2:4354932/1‑149 (MQ=255)
                  gTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCa                                                                                                                                  >  2:3171815/1‑149 (MQ=255)
                  gTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCa                                                                                                                                  >  1:2542745/1‑149 (MQ=255)
                   tAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCAc                                                                                                                                 <  1:2268977/149‑1 (MQ=255)
                    aaGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCAcc                                                                                                                                >  2:2400337/1‑149 (MQ=255)
                    aaGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCAcc                                                                                                                                <  2:2896862/149‑1 (MQ=255)
                      gAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGt                                                                                                                              >  2:1451878/1‑149 (MQ=255)
                        aaaGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGttt                                                                                                                            <  1:2359307/149‑1 (MQ=255)
                         aaGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTtc                                                                                                                           <  2:1618461/149‑1 (MQ=255)
                         aaGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTtc                                                                                                                           >  2:151842/1‑149 (MQ=255)
                         aaGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTtc                                                                                                                           >  2:152044/1‑149 (MQ=255)
                         aaGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTtc                                                                                                                           >  2:1523326/1‑149 (MQ=255)
                          aGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTtct                                                                                                                          <  1:692805/149‑1 (MQ=255)
                           gTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTtctc                                                                                                                         <  1:2785731/149‑1 (MQ=255)
                              aGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAAt                                                                                                                      >  1:2682498/1‑149 (MQ=255)
                              aGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAAt                                                                                                                      >  2:4553485/1‑149 (MQ=255)
                                 cgcTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGcc                                                                                                                   <  2:3682605/149‑1 (MQ=255)
                                  gcTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCg                                                                                                                  <  2:2503197/149‑1 (MQ=255)
                                  gcTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCg                                                                                                                  <  1:1136089/149‑1 (MQ=255)
                                   cTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGt                                                                                                                 <  2:2463107/149‑1 (MQ=255)
                                        tCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCATTGCCGTgcaat                                                                                                            <  1:3183391/149‑1 (MQ=255)
                                         cATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatg                                                                                                           <  1:4461366/149‑1 (MQ=255)
                                           tGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatggc                                                                                                         >  1:968149/1‑149 (MQ=255)
                                             gcggcTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatggcaa                                                                                                       <  2:4418165/149‑1 (MQ=255)
                                             gcggcTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTgcaatggcaa                                                                                                       >  1:3315481/1‑149 (MQ=255)
                                                   gTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCaaa                                                                                                 >  2:137187/1‑149 (MQ=255)
                                                      aGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaagaa                                                                                              <  1:754887/149‑1 (MQ=255)
                                                       ggTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaagaag                                                                                             <  2:182398/149‑1 (MQ=255)
                                                       ggTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAaagaag                                                                                             <  2:4051904/149‑1 (MQ=255)
                                                          gCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGc                                                                                          >  2:3239335/1‑149 (MQ=255)
                                                           cTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCa                                                                                         >  1:493932/1‑149 (MQ=255)
                                                                     aaGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAactg                                                                               >  2:1709892/1‑149 (MQ=255)
                                                                      aGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAactga                                                                              <  2:563063/149‑1 (MQ=255)
                                                                        aTAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAactgact                                                                            <  2:485668/149‑1 (MQ=255)
                                                                        aTAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAactgact                                                                            >  1:4318225/1‑149 (MQ=255)
                                                                         tAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAactgactg                                                                           <  1:1967371/149‑1 (MQ=255)
                                                                          aaCGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAActgactga                                                                          <  1:3368457/149‑1 (MQ=255)
                                                                                gtggtATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGAt                                                                    <  2:1691420/149‑1 (MQ=255)
                                                                                 tggtATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGAtt                                                                   <  2:2367101/149‑1 (MQ=255)
                                                                                  ggtATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTc                                                                  <  1:814367/149‑1 (MQ=255)
                                                                                  ggtATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTc                                                                  <  1:3680564/149‑1 (MQ=255)
                                                                                   gtATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCt                                                                 <  1:3130385/149‑1 (MQ=255)
                                                                                     aTTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTgg                                                               <  2:468490/149‑1 (MQ=255)
                                                                                      tttGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGc                                                              >  1:532139/1‑149 (MQ=255)
                                                                                      tttGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGc                                                              >  2:4302971/1‑149 (MQ=255)
                                                                                           ccGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTtgc                                                         <  2:4617467/149‑1 (MQ=255)
                                                                                            cgcgTGCTGACTCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTtgcc                                                        <  2:3824800/149‑1 (MQ=255)
                                                                                            cgcgTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTtgcc                                                        <  1:2130466/149‑1 (MQ=255)
                                                                                            cgcgTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTtgcc                                                        <  1:4471061/149‑1 (MQ=255)
                                                                                                 gCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCa                                                   >  2:3528030/1‑149 (MQ=255)
                                                                                                 gCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCa                                                   >  2:2743328/1‑149 (MQ=255)
                                                                                                 gCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCa                                                   <  2:1072935/149‑1 (MQ=255)
                                                                                                  cTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCAt                                                  <  2:4177825/149‑1 (MQ=255)
                                                                                                     aaTCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTc                                               >  1:3987966/1‑149 (MQ=255)
                                                                                                      aTCAGTAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCg                                              >  1:156390/1‑149 (MQ=255)
                                                                                                       tCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCgg                                             <  1:3032966/149‑1 (MQ=255)
                                                                                                         aGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCt                                           <  1:561748/149‑1 (MQ=255)
                                                                                                          gCAACGTGGACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTa                                          <  1:4592963/149‑1 (MQ=255)
                                                                                                            aaCGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACg                                        <  1:2954212/149‑1 (MQ=255)
                                                                                                             aCGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACgg                                       <  2:821501/149‑1 (MQ=255)
                                                                                                               gtgtACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTa                                     <  1:3933423/149‑1 (MQ=255)
                                                                                                               gtgtACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTa                                     <  1:151764/149‑1 (MQ=255)
                                                                                                                tgtACTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTAt                                    <  2:971746/149‑1 (MQ=255)
                                                                                                                tgtACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTAt                                    <  2:431971/149‑1 (MQ=255)
                                                                                                                    cTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGAt                                <  1:2476922/149‑1 (MQ=255)
                                                                                                                    cTGGTGGCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGAt                                <  2:2008250/149‑1 (MQ=255)
                                                                                                                        tggCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAAc                            <  1:640426/149‑1 (MQ=255)
                                                                                                                         ggCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACt                           >  2:3507344/1‑149 (MQ=255)
                                                                                                                         ggCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACt                           >  2:2923721/1‑149 (MQ=255)
                                                                                                                         ggCAATCAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACt                           >  2:3218764/1‑149 (MQ=255)
                                                                                                                          gCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTa                          >  1:1473595/1‑149 (MQ=255)
                                                                                                                          gCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTa                          <  1:2901105/149‑1 (MQ=255)
                                                                                                                               cAACCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGtt                     <  1:2963560/149‑1 (MQ=255)
                                                                                                                                aaCCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGttt                    <  1:3421223/149‑1 (MQ=255)
                                                                                                                                 aCCGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGtttt                   <  2:4115619/149‑1 (MQ=255)
                                                                                                                                 aCCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGtttt                   >  2:793505/1‑149 (MQ=255)
                                                                                                                                 aCCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGtttt                   >  1:4222582/1‑149 (MQ=255)
                                                                                                                                  ccGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTta                  >  2:4243763/1‑149 (MQ=255)
                                                                                                                                  ccGTGGCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTta                  >  2:2103532/1‑149 (MQ=255)
                                                                                                                                    gTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTtata                <  2:1339056/149‑1 (MQ=255)
                                                                                                                                      ggCGAGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAAc              <  2:1138026/149‑1 (MQ=255)
                                                                                                                                      ggCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAAc              <  1:4516704/149‑1 (MQ=255)
                                                                                                                                        cGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGc            <  2:2945062/149‑1 (MQ=255)
                                                                                                                                          aGGCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCt          >  1:1526007/1‑149 (MQ=255)
                                                                                                                                          aGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCt          <  2:2377117/149‑1 (MQ=255)
                                                                                                                                           ggCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCtt         <  2:2281413/149‑1 (MQ=255)
                                                                                                                                            gCCTGTTAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTg        >  1:2996717/1‑149 (MQ=255)
                                                                                                                                                 tgAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGa   <  1:1453722/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AGCGAATGATTGCGCTGCGTAAGAAAAGTCAGGCGCTACGTCATGGCGGCTGTCAGGTGCTGTATGCGGAAGATAACGTGGTGGTATTTGTCCGCGTGCTGAATCAGCAACGTGTACTGGTGGCAATCAACCGTGGCGAGGCCTGTGAAGTGGTGCTACCCGCGTCACCGTTTCTCAATGCCGTGCAATGGCAATGCAAAGAAGGGCATGGGCAACTGACTGACGGGATTCTGGCTTTGCCTGCCATTTCGGCTACGGTATGGATGAACTAACGTTTTATAACGCCTTGCGTGAC  >  CP009273/419517‑419811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑