Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 43,550 G→A 5.8% A121A (GCG→GCA)  fixB → putative electron transfer flavoprotein, NAD/FAD‑binding domain and ETFP adenine nucleotide‑binding domain‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927343,5500GA 5.8% 1117.8 / 35.6 310A121A (GCG→GCA) fixBputative electron transfer flavoprotein, NAD/FAD‑binding domain and ETFP adenine nucleotide‑binding domain‑like protein
Reads supporting (aligned to +/- strand):  ref base G (150/141);  new base A (6/12);  total (157/153)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 313 total aligned reads displayed.

CTGACACTATTCGCCAGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGAAACGCACACCGTGGAGTGGC  >  CP009273/43402‑43698
                                                                                                                                                    |                                                                                                                                                    
cTGACACTATTCGCCAGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCg                                                                                                                                                      >  1:2952207/1‑149 (MQ=255)
 tGACACTATTCGCCAGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGa                                                                                                                                                     >  2:656228/1‑149 (MQ=255)
 tGACACTATTCGCCAGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGa                                                                                                                                                     <  2:4546503/149‑1 (MQ=255)
 tGACACTATTCGCCAGCACGGCGCAGACGGCCTGGTGCTGCTGCAAAACCCCCGTCGCGGAAAATTACTGGCGGAAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGa                                                                                                                                                     <  1:1300822/149‑1 (MQ=255)
  gACACTATTCGCCCGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGAc                                                                                                                                                    <  1:4219936/149‑1 (MQ=255)
   acacTATTCGCCAGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACa                                                                                                                                                   >  1:1708879/1‑149 (MQ=255)
    cacTATTCGCCAGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAg                                                                                                                                                  >  2:1556374/1‑149 (MQ=255)
    cacTATTCGCCAGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAg                                                                                                                                                  >  1:1724381/1‑149 (MQ=255)
      cTATTCGCCAGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTg                                                                                                                                                >  2:4027714/1‑149 (MQ=255)
        aTTCGCCAGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGaa                                                                                                                                              >  1:2497338/1‑149 (MQ=255)
         ttCGCCAGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGaaa                                                                                                                                             >  1:1198833/1‑149 (MQ=255)
           cGCCAGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAaca                                                                                                                                           >  2:1257566/1‑149 (MQ=255)
           cGCCAGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAaca                                                                                                                                           >  2:3398490/1‑149 (MQ=255)
             ccAGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAAcaca                                                                                                                                         >  2:3712409/1‑149 (MQ=255)
             ccAGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCAACAGTGAAAcaca                                                                                                                                         <  1:242306/149‑1 (MQ=255)
              cAGCACGGCGCTGACGGCCTGGTGCTGCTGCCAAACCCCCGTCGCGGCAAATTACTGGCGGCAAACCTGGGTTATCGCCTTAAAGCGGCGGTGTCTACCGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACAt                                                                                                                                        <  1:1944250/149‑1 (MQ=255)
               aGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATg                                                                                                                                       >  1:3034847/1‑149 (MQ=255)
                  aCGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGtt                                                                                                                                    <  2:3046255/149‑1 (MQ=255)
                  aCGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGAAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGtt                                                                                                                                    <  2:1380771/149‑1 (MQ=255)
                   cGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGttt                                                                                                                                   <  2:1053753/149‑1 (MQ=255)
                    ggCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTa                                                                                                                                  >  1:3277227/1‑149 (MQ=255)
                          gACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACggtgg                                                                                                                            >  2:4637098/1‑149 (MQ=255)
                           aCGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGAGGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACggtggt                                                                                                                           <  2:2451118/149‑1 (MQ=255)
                             ggCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCt                                                                                                                         <  2:729023/149‑1 (MQ=255)
                                cTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATCACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGc                                                                                                                      <  2:2161493/149‑1 (MQ=255)
                                  ggTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGTTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGa                                                                                                                    <  2:3861831/149‑1 (MQ=255)
                                   gTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGATGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGAt                                                                                                                   <  1:350685/149‑1 (MQ=255)
                                   gTGCTGCTGCCAAACACCCGTCGCGGCAAATTACGGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGAt                                                                                                                   <  2:3219499/149‑1 (MQ=255)
                                     gctgctgcCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTg                                                                                                                 >  2:2388232/1‑149 (MQ=255)
                                             cAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCgaagaa                                                                                                         <  2:4384871/149‑1 (MQ=255)
                                                acacCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGc                                                                                                      >  2:2745516/1‑149 (MQ=255)
                                                acacCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCAACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGc                                                                                                      <  2:4308197/149‑1 (MQ=255)
                                                 cacCCGTCGCGGCAAATTACGGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCa                                                                                                     <  2:840256/149‑1 (MQ=255)
                                                  acCCGTCGCGGAAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCAt                                                                                                    <  1:1423831/149‑1 (MQ=255)
                                                      gTCGCGGCAAATTCCTGGCGGCAAACCTGGGTTATCGCCTAAAAGCGGCGGTGTCTAACGAGGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGcc                                                                                                <  1:3228263/149‑1 (MQ=255)
                                                      gTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGcc                                                                                                <  1:1848970/149‑1 (MQ=255)
                                                       gcgcgGAAAATTACTGGCGCCAAATCGGGTTTATCGCATTAACGCGGCGGTGTCTACCGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCa                                                                                               <  1:2179328/148‑1 (MQ=255)
                                                        cgcgGCAAATTACGGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCAc                                                                                              <  1:176589/149‑1 (MQ=255)
                                                           ggaaatttACTGGCGGAAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGcc                                                                                           <  1:3822808/143‑1 (MQ=255)
                                                           ggCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGcc                                                                                           >  2:1745344/1‑149 (MQ=255)
                                                              aaaTAACTGGCGGAAAAACTGGGTTATCGCCTTAACGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTa                                                                                        <  1:1681164/149‑1 (MQ=255)
                                                               aaTTACTGGCGGCAAACCTGGGTTATCGCCTTAACGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTAt                                                                                       <  1:3387189/149‑1 (MQ=255)
                                                               aaTTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGACGAACGCATTGCCACGCCGTAt                                                                                       >  2:4650173/1‑149 (MQ=255)
                                                               aaTTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTAt                                                                                       >  2:849382/1‑149 (MQ=255)
                                                                aTTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATg                                                                                      >  1:1239965/1‑149 (MQ=255)
                                                                   aCTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCAACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCgg                                                                                   >  1:1324952/1‑149 (MQ=255)
                                                                     gggcgccAAAACTGGTTTATCGCCTTAACGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTa                                                                                 <  1:2132524/148‑1 (MQ=255)
                                                                       gcggcAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACt                                                                               <  2:1598660/149‑1 (MQ=255)
                                                                         ggcAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGa                                                                             >  2:1411288/1‑149 (MQ=255)
                                                                            aaacgtGGGTTATCGCCTTACCGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCa                                                                          <  1:844985/144‑1 (MQ=255)
                                                                             aaaCTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCAt                                                                         >  1:1863160/1‑149 (MQ=255)
                                                                              aaCTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATc                                                                        >  2:3746657/1‑149 (MQ=255)
                                                                              aaCTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCAACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATc                                                                        <  2:696216/149‑1 (MQ=255)
                                                                                   ggTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATcagcag                                                                   >  1:2734606/1‑149 (MQ=255)
                                                                                   ggTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATcagcag                                                                   >  1:393123/1‑149 (MQ=255)
                                                                                     ttATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCAACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCg                                                                 <  2:2654739/149‑1 (MQ=255)
                                                                                     ttAACGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCCGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGAGTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCg                                                                 >  2:2330084/1‑149 (MQ=255)
                                                                                        tCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCa                                                              >  2:1427797/1‑149 (MQ=255)
                                                                                        tCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCa                                                              >  1:1489339/1‑149 (MQ=255)
                                                                                          gCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACg                                                            >  1:1003850/1‑149 (MQ=255)
                                                                                           ccTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGt                                                           >  1:3696093/1‑149 (MQ=255)
                                                                                           ccTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGt                                                           <  2:4109797/149‑1 (MQ=255)
                                                                                             ttAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTCCGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTc                                                         >  2:2938693/1‑149 (MQ=255)
                                                                                             ttAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTc                                                         >  1:4082231/1‑149 (MQ=255)
                                                                                             ttAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTc                                                         >  2:586185/1‑149 (MQ=255)
                                                                                                  gcggcgGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAGCGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGc                                                    >  2:2609965/1‑149 (MQ=255)
                                                                                                  gcggcgGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGc                                                    <  1:4443272/149‑1 (MQ=255)
                                                                                                        gtgtCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCa                                              <  2:358852/149‑1 (MQ=255)
                                                                                                          gtCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGAGTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGc                                            >  2:491386/1‑149 (MQ=255)
                                                                                                           tCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCAACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGcc                                           >  1:622355/1‑149 (MQ=255)
                                                                                                            cTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCa                                          >  2:2898089/1‑149 (MQ=255)
                                                                                                              aaCGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGa                                        <  1:3399133/149‑1 (MQ=255)
                                                                                                              aaCGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGa                                        >  1:90148/1‑149 (MQ=255)
                                                                                                               aCGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGAc                                       >  1:4113340/1‑149 (MQ=255)
                                                                                                               aCGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGAc                                       <  1:2313343/149‑1 (MQ=255)
                                                                                                                 gATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGAcgc                                     <  1:4515147/149‑1 (MQ=255)
                                                                                                                  aTGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGAcgcg                                    <  1:56901/149‑1 (MQ=255)
                                                                                                                     ccAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACCTGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCa                                 <  2:626720/149‑1 (MQ=255)
                                                                                                                       aGCACCGTCAGTGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACg                               >  1:2667086/1‑149 (MQ=255)
                                                                                                                       aGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACg                               >  1:805533/1‑149 (MQ=255)
                                                                                                                       aGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGACACg                               >  1:3143838/1‑149 (MQ=255)
                                                                                                                          aCCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCAc                            <  2:3508674/149‑1 (MQ=255)
                                                                                                                           ccGTCAGCGTACAGGACGGTAAAGCAACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACt                           <  2:1324952/149‑1 (MQ=255)
                                                                                                                             gTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTgg                         >  2:4086784/1‑149 (MQ=255)
                                                                                                                              tCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGc                        >  1:3113231/1‑149 (MQ=255)
                                                                                                                                 gCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGaa                     >  2:707744/1‑149 (MQ=255)
                                                                                                                                  cGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGaaa                    <  2:15852/149‑1 (MQ=255)
                                                                                                                                  cGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGaaa                    <  2:16252/149‑1 (MQ=255)
                                                                                                                                   gTACAGGACGGTAAAGCAACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGAAAc                   <  2:2520428/149‑1 (MQ=255)
                                                                                                                                     aCAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGAAACGc                 >  1:4068264/1‑149 (MQ=255)
                                                                                                                                      cAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGAAACGca                >  2:3763544/1‑149 (MQ=255)
                                                                                                                                      cAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGAAACGca                >  2:2000696/1‑149 (MQ=255)
                                                                                                                                         gACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGAAACGCacac             >  2:3947523/1‑149 (MQ=255)
                                                                                                                                          aCGGTAAAGCAACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGAAACGCACAcc            >  1:4526715/1‑149 (MQ=255)
                                                                                                                                                aaGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGAAACGCACACCGTGGAg      <  1:3766482/149‑1 (MQ=255)
                                                                                                                                                aaGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGAAACGCACACCGTGGAg      <  1:3480956/149‑1 (MQ=255)
                                                                                                                                                 aGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGAAACGCACACCGTGGAGt     >  2:4483109/1‑149 (MQ=255)
                                                                                                                                                 aGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGAAACGCACACCGTGGAGt     <  1:2789136/149‑1 (MQ=255)
                                                                                                                                                 aGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACGGGCGAAACGCACACCGTGGAGt     <  2:3113303/149‑1 (MQ=255)
                                                                                                                                                    gACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGAAACGCACACCGTGGAGTGGc  <  2:2497338/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGACACTATTCGCCAGCACGGCGCAGACGGCCTGGTGCTGCTGCCAAACACCCGTCGCGGCAAATTACTGGCGGCAAAACTGGGTTATCGCCTTAAAGCGGCGGTGTCTAACGATGCCAGCACCGTCAGCGTACAGGACGGTAAAGCGACAGTGAAACACATGGTTTACGGTGGTCTGGCGATTGGCGAAGAACGCATTGCCACGCCGTATGCGGTACTGACCATCAGCAGCGGCACGTTCGATGCGGCTCAGCCAGACGCGTCACGCACTGGCGAAACGCACACCGTGGAGTGGC  >  CP009273/43402‑43698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑