Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 662,631 C→A 78.5% R348L (CGC→CTC)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273662,6310CA78.5% 671.8 / 247.8 325R348L (CGC→CTC) mrdAtranspeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
Reads supporting (aligned to +/- strand):  ref base C (23/47);  new base A (102/153);  total (125/200)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

Only 100 of 329 total aligned reads displayed.

AGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTC  >  CP009273/662485‑662779
                                                                                                                                                  |                                                                                                                                                    
  gCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGt                                                                                                                                                  >  2:1964593/1‑149 (MQ=255)
   ccACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTg                                                                                                                                                 <  1:3477130/149‑1 (MQ=255)
      ccTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATc                                                                                                                                              <  1:1003282/149‑1 (MQ=255)
      ccTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATc                                                                                                                                              <  2:3185506/149‑1 (MQ=255)
          aTAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCAccc                                                                                                                                          <  2:4232581/149‑1 (MQ=255)
             gaagaaGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCgg                                                                                                                                       >  2:2316679/1‑149 (MQ=255)
              aagaagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGgc                                                                                                                                      >  1:1456981/1‑149 (MQ=255)
              aagaagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGgc                                                                                                                                      >  2:248131/1‑149 (MQ=255)
                gaagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGgcgc                                                                                                                                    >  2:4360029/1‑149 (MQ=255)
                 aagGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCt                                                                                                                                   <  2:804740/149‑1 (MQ=255)
                  agGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTc                                                                                                                                  <  1:323830/149‑1 (MQ=255)
                  agGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTc                                                                                                                                  >  2:2833699/1‑149 (MQ=255)
                  agGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTc                                                                                                                                  <  1:3026715/149‑1 (MQ=255)
                      aTCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATg                                                                                                                              >  1:4743836/1‑149 (MQ=255)
                       tCCGCAGGTTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGc                                                                                                                             >  2:1821333/1‑149 (MQ=255)
                       tCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGc                                                                                                                             <  1:2682760/149‑1 (MQ=255)
                       tCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGc                                                                                                                             >  1:2604374/1‑149 (MQ=255)
                       tCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGc                                                                                                                             <  2:67709/149‑1 (MQ=255)
                             gATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAAc                                                                                                                       >  2:479401/1‑149 (MQ=255)
                              aTTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAAcc                                                                                                                      <  1:4659577/149‑1 (MQ=255)
                                  ttcCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCca                                                                                                                  >  1:2171045/1‑149 (MQ=255)
                                     cAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACAt                                                                                                               <  2:5208710/149‑1 (MQ=255)
                                      aGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATa                                                                                                              >  2:2423188/1‑149 (MQ=255)
                                      aGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATa                                                                                                              <  1:2661715/149‑1 (MQ=255)
                                      aGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATa                                                                                                              <  2:4594936/149‑1 (MQ=255)
                                          aTCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGt                                                                                                          >  2:4502352/1‑149 (MQ=255)
                                           tCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGtt                                                                                                         <  1:28749/149‑1 (MQ=255)
                                           tCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGtt                                                                                                         <  1:5090710/149‑1 (MQ=255)
                                           tCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGtt                                                                                                         >  1:2021597/1‑149 (MQ=255)
                                            cTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGttt                                                                                                        <  2:3956748/149‑1 (MQ=255)
                                                  aCATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGt                                                                                                  >  2:3034890/1‑149 (MQ=255)
                                                   cATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTa                                                                                                 <  1:2270673/149‑1 (MQ=255)
                                                     ttCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGa                                                                                               <  1:916149/149‑1 (MQ=255)
                                                     ttCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGa                                                                                               <  1:416882/149‑1 (MQ=255)
                                                     ttCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTATTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGa                                                                                               <  2:3751036/149‑1 (MQ=255)
                                                          aCGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCgg                                                                                          <  1:651585/149‑1 (MQ=255)
                                                          aCGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCgg                                                                                          <  2:144327/149‑1 (MQ=255)
                                                          aCGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCgg                                                                                          <  2:2164030/149‑1 (MQ=255)
                                                            gCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGgga                                                                                        <  2:5178268/149‑1 (MQ=255)
                                                              ccGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGggagg                                                                                      <  1:1053462/149‑1 (MQ=255)
                                                                   gCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATaaa                                                                                 >  2:1742063/1‑149 (MQ=255)
                                                                     cccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAcc                                                                               >  1:1882278/1‑149 (MQ=255)
                                                                      ccATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAccc                                                                              >  2:4509520/1‑149 (MQ=255)
                                                                        aTTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAAccccc                                                                            >  1:1482641/1‑149 (MQ=255)
                                                                         ttttttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCGCCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCt                                                                           >  1:2416632/1‑149 (MQ=255)
                                                                         ttttttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCt                                                                           >  1:4919884/1‑149 (MQ=255)
                                                                         ttttttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCt                                                                           <  2:4040402/149‑1 (MQ=255)
                                                                         ttttttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCt                                                                           <  1:1231107/149‑1 (MQ=255)
                                                                          tttttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtg                                                                          <  2:221953/149‑1 (MQ=255)
                                                                           ttttCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgt                                                                         <  1:3628076/149‑1 (MQ=255)
                                                                              tCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCtgtgtg                                                                      <  1:525655/149‑1 (MQ=255)
                                                                                   tCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGcgcg                                                                 >  2:3560297/1‑149 (MQ=255)
                                                                                    cACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTCACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCgg                                                                <  2:2736096/149‑1 (MQ=255)
                                                                                       gATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTc                                                             >  2:2511584/1‑149 (MQ=255)
                                                                                       gATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGACAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTc                                                             <  1:2922628/149‑1 (MQ=255)
                                                                                          aaCGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCAcc                                                          >  2:1371420/1‑149 (MQ=255)
                                                                                          aaCGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCAcc                                                          <  1:4240736/149‑1 (MQ=255)
                                                                                           aCGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCa                                                         <  1:1811023/149‑1 (MQ=255)
                                                                                           aCGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCa                                                         <  2:2089484/149‑1 (MQ=255)
                                                                                           aCGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCa                                                         <  2:4705580/149‑1 (MQ=255)
                                                                                             gTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGc                                                       >  1:4093303/1‑149 (MQ=255)
                                                                                             gTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGc                                                       >  2:1224880/1‑149 (MQ=255)
                                                                                              tttttCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCg                                                      <  1:859041/149‑1 (MQ=255)
                                                                                              tttttCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCg                                                      <  1:3940849/149‑1 (MQ=255)
                                                                                              tttttCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCg                                                      >  2:3792458/1‑149 (MQ=255)
                                                                                              tttttCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCg                                                      <  2:4435474/149‑1 (MQ=255)
                                                                                                 ttCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGgtg                                                   >  2:50970/1‑149 (MQ=255)
                                                                                                 ttCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGgtg                                                   >  2:3413123/1‑149 (MQ=255)
                                                                                                  tCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGgtgt                                                  <  1:1629109/149‑1 (MQ=255)
                                                                                                   ccGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTa                                                 >  1:2634891/1‑149 (MQ=255)
                                                                                                   ccGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTa                                                 >  1:532583/1‑149 (MQ=255)
                                                                                                    cGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTAt                                                <  1:3988206/149‑1 (MQ=255)
                                                                                                    cGAACCTGGCAGGTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTAt                                                <  1:1934250/149‑1 (MQ=255)
                                                                                                     gAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTAtt                                               <  2:5006475/149‑1 (MQ=255)
                                                                                                      aaCCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTc                                              >  2:84646/1‑149 (MQ=255)
                                                                                                      aaCCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTc                                              <  2:1882278/149‑1 (MQ=255)
                                                                                                       aCCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCg                                             <  2:2371594/149‑1 (MQ=255)
                                                                                                           ggCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATc                                         >  2:297326/1‑149 (MQ=255)
                                                                                                           ggCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCCGCGGTGTATTCGGATc                                         >  1:2361392/1‑149 (MQ=255)
                                                                                                           ggCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATc                                         <  2:1606019/149‑1 (MQ=255)
                                                                                                              aGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGtt                                      <  2:3859441/149‑1 (MQ=255)
                                                                                                               gTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTc                                     <  2:971/149‑1 (MQ=255)
                                                                                                                  gCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTcaac                                  <  1:1169975/149‑1 (MQ=255)
                                                                                                                  gCCACCAGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTcaac                                  >  1:3676850/1‑149 (MQ=255)
                                                                                                                   ccaccaGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTcaaca                                 <  2:3626529/149‑1 (MQ=255)
                                                                                                                    caccaGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTcaacaa                                <  2:1998085/149‑1 (MQ=255)
                                                                                                                     accaGCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAg                               >  1:4301431/1‑149 (MQ=255)
                                                                                                                         gCCTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCgg                           >  1:761254/1‑149 (MQ=255)
                                                                                                                          ccTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGa                          <  1:3826297/149‑1 (MQ=255)
                                                                                                                           cTGGGTCAAACAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGaa                         <  2:309355/149‑1 (MQ=255)
                                                                                                                                 cAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCt                   <  2:1102411/149‑1 (MQ=255)
                                                                                                                                   aaCAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCttt                 >  1:4730170/1‑149 (MQ=255)
                                                                                                                                    aCAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTg                >  1:4372802/1‑149 (MQ=255)
                                                                                                                                    aCAGCGTCGTATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTg                <  2:275585/149‑1 (MQ=255)
                                                                                                                                        cgtcgtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCAAATAGGGTTTAACTGTAGACGCGGGAGGATATACCCCCTGTTTGGCGCGGTTCACCAGCGGTGTATTCGTATCGTTCAACAAGGCGGAATAATCTTTGCTgg            <  2:1422514/149‑1 (MQ=255)
                                                                                                                                           cgtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGgaga         <  1:1921512/149‑1 (MQ=255)
                                                                                                                                            gtATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGAt        <  1:61410/149‑1 (MQ=255)
                                                                                                                                             tATTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATa       <  1:1514035/149‑1 (MQ=255)
                                                                                                                                              aTTGAGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATAc      <  1:975881/149‑1 (MQ=255)
                                                                                                                                                  cgcgTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTc  <  1:2982366/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AGGCCACCTGATAGAAGAAGGTATCCGCAGATTCTTCCAGCGATCTTGTGACATTCAGACGCCCGTGGCCCCATTTTTTCCAGTCACGATAACGTTTTTCCGAACCTGGCAGTTGCCACCAGCCTGGGTCAAACAGCGTCGTATTGCGCGTGATCACCCCGGCGCTCAATGCCGAAACCGCCACATAGGGTTTAACTGTAGACGCGGGAGGATAAACCCCCTGTGTGGCGCGGTTCACCAGCGGTGTATTCGGATCGTTCAACAAGGCGGAATAATCTTTGCTGGAGATACCGTC  >  CP009273/662485‑662779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑