Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 800,779 G→T 92.1% E607D (GAG→GAT)  ybhJ → putative hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273800,7790GT92.1% 906.1 / 66.8 290E607D (GAG→GAT) ybhJputative hydratase
Reads supporting (aligned to +/- strand):  ref base G (12/11);  new base T (134/133);  total (146/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.07e-01

Only 100 of 295 total aligned reads displayed.

GGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTG  >  CP009273/800633‑800926
                                                                                                                                                  |                                                                                                                                                   
ggTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATgt                                                                                                                                                   >  2:3369986/1‑149 (MQ=255)
  tGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGgtgt                                                                                                                                                 >  1:1669640/1‑149 (MQ=255)
  tGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGgtgt                                                                                                                                                 >  1:1884787/1‑149 (MQ=255)
   gAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGtt                                                                                                                                                <  2:3765665/149‑1 (MQ=255)
   gAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGtt                                                                                                                                                >  1:2803500/1‑149 (MQ=255)
    aaaCCTCTTCTTATCGTTCAAATCCTATTGTTGTGGGGGTTTTTACCCTTACTCGCCGCGATCCGGTTTTTGTTAGAAGACTTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGttt                                                                                                                                               <  1:5179909/149‑1 (MQ=255)
    aaaCCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGttt                                                                                                                                               >  2:3395607/1‑149 (MQ=255)
    aaaCCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGttt                                                                                                                                               >  1:1608349/1‑149 (MQ=255)
    aaaCCTATTCTTATCGTAAAAATCCGTTTGGTCTGGCGGATTTTACCGTGTCTCGCCGCGATCCGGGTTTTGTTAGCAGAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGttt                                                                                                                                               <  1:4484148/149‑1 (MQ=255)
      aCCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgc                                                                                                                                             <  2:1020135/149‑1 (MQ=255)
      aCCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgc                                                                                                                                             >  2:4735299/1‑149 (MQ=255)
       ccTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgcg                                                                                                                                            >  2:1947378/1‑149 (MQ=255)
       ccTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgcg                                                                                                                                            >  2:4563489/1‑149 (MQ=255)
         tcttctTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgcgcg                                                                                                                                          >  1:430235/1‑149 (MQ=255)
         tcttctTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTgcgcg                                                                                                                                          >  1:1752719/1‑149 (MQ=255)
           ttcttATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGGTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCa                                                                                                                                        >  2:1178882/1‑149 (MQ=255)
            tcttATCGTTCAAATCCGATTGGTCTGGCGGTGTTTACCCTGTCTCGCCGCGCTCCGGGTTATGTTAGCAGAATTAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCAt                                                                                                                                       <  2:2282747/149‑1 (MQ=255)
               tATCGTTCAAATCCTATTGGTCTGGGGGAGTTTACCCTGTCTCGCCGGGATCCCGGTTATGTTAGCAGAAGTACAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTaa                                                                                                                                    <  2:4868652/149‑1 (MQ=255)
               tATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTaa                                                                                                                                    >  1:1985916/1‑149 (MQ=255)
                aTCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAg                                                                                                                                   <  2:3025037/149‑1 (MQ=255)
                 tCGTTCAAATCCGATTGGTCTGGCGTAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGc                                                                                                                                  <  2:59375/149‑1 (MQ=255)
                      cAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGAtt                                                                                                                             <  2:232639/149‑1 (MQ=255)
                        aaTCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGc                                                                                                                           >  1:501344/1‑149 (MQ=255)
                        aaTCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGc                                                                                                                           >  2:4091013/1‑149 (MQ=255)
                          tCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTg                                                                                                                         >  2:5142023/1‑149 (MQ=255)
                          tCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTg                                                                                                                         >  2:1737746/1‑149 (MQ=255)
                           ccGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTgg                                                                                                                        >  2:752146/1‑149 (MQ=255)
                            cGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGt                                                                                                                       >  2:3105062/1‑149 (MQ=255)
                              aTTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCa                                                                                                                     >  1:3315823/1‑149 (MQ=255)
                               ttGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAg                                                                                                                    >  2:3490019/1‑149 (MQ=255)
                               ttGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAg                                                                                                                    >  1:3634689/1‑149 (MQ=255)
                               ttGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAg                                                                                                                    >  1:2563758/1‑149 (MQ=255)
                                            gTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATc                                                                                                       >  1:2732258/1‑149 (MQ=255)
                                            gTTTACCCTGTCGCGCCGCGATCCCGTTTTTTTTAGCATATTTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATc                                                                                                       <  1:187240/149‑1 (MQ=255)
                                               tACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGc                                                                                                    <  2:2237909/149‑1 (MQ=255)
                                               tACCCGGTCTCGCCGCGATCCCGGTTATGGTAGCATAATTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGc                                                                                                    <  2:3190518/149‑1 (MQ=255)
                                                          gccgcGATCCCGGTTTTGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGaa                                                                                         <  1:2896175/149‑1 (MQ=255)
                                                          gccgcGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGaa                                                                                         >  1:4765673/1‑149 (MQ=255)
                                                          gccgcGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGaa                                                                                         >  1:4765115/1‑149 (MQ=255)
                                                            cgcgATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAAt                                                                                       <  2:2842274/149‑1 (MQ=255)
                                                              cgATCCCGTTTATGTTCGCAGAATAAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTg                                                                                     <  1:1079297/149‑1 (MQ=255)
                                                               taTCCGGGTTATGTTACCAGATTTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTgg                                                                                    <  1:1030665/148‑1 (MQ=255)
                                                               gATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTgg                                                                                    >  2:4116517/1‑149 (MQ=255)
                                                                 tCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGgca                                                                                  >  1:2687333/1‑149 (MQ=255)
                                                                     ggTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAACTTGGCAGCAt                                                                              >  2:4152784/1‑149 (MQ=255)
                                                                     ggTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCAt                                                                              <  2:1873277/149‑1 (MQ=255)
                                                                      gTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATg                                                                             <  1:3040503/149‑1 (MQ=255)
                                                                       ttATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATgg                                                                            >  2:63849/1‑149 (MQ=255)
                                                                            ttAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTAt                                                                       >  2:4950247/1‑149 (MQ=255)
                                                                            ttAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTAt                                                                       >  1:2377372/1‑149 (MQ=255)
                                                                            ttAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTAt                                                                       <  2:1093428/149‑1 (MQ=255)
                                                                              aGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGc                                                                     <  2:1593838/149‑1 (MQ=255)
                                                                               gCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCg                                                                    >  1:866697/1‑149 (MQ=255)
                                                                                  taataaaaGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTg                                                                 <  1:4642992/144‑1 (MQ=255)
                                                                                  gAAGTAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTg                                                                 <  2:1515879/149‑1 (MQ=255)
                                                                                  gAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTg                                                                 >  2:4629077/1‑149 (MQ=255)
                                                                                    aGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGaa                                                               >  1:4017444/1‑149 (MQ=255)
                                                                                     gTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGaaa                                                              >  2:3301406/1‑149 (MQ=255)
                                                                                      tAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAAc                                                             <  1:369486/149‑1 (MQ=255)
                                                                                      tAACGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAAc                                                             <  1:678734/149‑1 (MQ=255)
                                                                                       aaaGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAAcc                                                            <  2:214281/149‑1 (MQ=255)
                                                                                       aaaGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAAcc                                                            >  1:4228801/1‑149 (MQ=255)
                                                                                          gCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAgg                                                         >  2:4335039/1‑149 (MQ=255)
                                                                                                gctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATgg                                                   >  2:4843515/1‑149 (MQ=255)
                                                                                                 ctgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGt                                                  <  2:124391/149‑1 (MQ=255)
                                                                                                  tgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGtt                                                 >  1:2433504/1‑149 (MQ=255)
                                                                                                  tgAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGtt                                                 <  2:4343261/149‑1 (MQ=255)
                                                                                                   gAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTc                                                >  2:4490057/1‑149 (MQ=255)
                                                                                                    aGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCt                                               >  1:3454481/1‑149 (MQ=255)
                                                                                                         gAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGcgcg                                          >  2:444539/1‑149 (MQ=255)
                                                                                                           aaaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTg                                        <  2:484541/149‑1 (MQ=255)
                                                                                                            aaTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGa                                       >  2:2733408/1‑149 (MQ=255)
                                                                                                             aTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGaa                                      >  2:5049472/1‑149 (MQ=255)
                                                                                                             aTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGaa                                      <  1:4912327/149‑1 (MQ=255)
                                                                                                             aTCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGaa                                      <  1:2666840/149‑1 (MQ=255)
                                                                                                               cAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACa                                    <  2:2020740/149‑1 (MQ=255)
                                                                                                                aGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGATGAAACCAGGCGATGGTTCTGCGCGTGAACAg                                   >  1:4134614/1‑149 (MQ=255)
                                                                                                                 gCGTCTGGCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAgg                                  >  1:2956501/1‑149 (MQ=255)
                                                                                                                       ggCGGGGAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGa                            <  1:2052599/149‑1 (MQ=255)
                                                                                                                          ggggAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCt                         >  2:1374409/1‑149 (MQ=255)
                                                                                                                             gAATGTCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGcc                      >  1:510893/1‑149 (MQ=255)
                                                                                                                                  tCAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCgt                 <  1:2988363/149‑1 (MQ=255)
                                                                                                                                   cAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCgtg                >  2:904200/1‑149 (MQ=255)
                                                                                                                                   cAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCgtg                <  1:486750/149‑1 (MQ=255)
                                                                                                                                   cAGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCgtg                <  2:2147473/149‑1 (MQ=255)
                                                                                                                                    aGCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCgtgt               >  1:5156951/1‑149 (MQ=255)
                                                                                                                                     gCGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGtgtg              >  2:527190/1‑149 (MQ=255)
                                                                                                                                      cGAGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGa             <  1:1299494/149‑1 (MQ=255)
                                                                                                                                        aGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAtt           <  1:3369986/149‑1 (MQ=255)
                                                                                                                                        aGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAtt           >  1:3492477/1‑149 (MQ=255)
                                                                                                                                        aGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAtt           >  1:3938242/1‑149 (MQ=255)
                                                                                                                                        aGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAtt           <  1:2744374/149‑1 (MQ=255)
                                                                                                                                        aGCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGAtt           >  1:4052067/1‑149 (MQ=255)
                                                                                                                                         gCTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTg          <  1:1168556/149‑1 (MQ=255)
                                                                                                                                          cTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTgg         <  2:3704921/149‑1 (MQ=255)
                                                                                                                                          cTGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTgg         >  1:4603815/1‑149 (MQ=255)
                                                                                                                                           tGACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGc        >  2:2605732/1‑149 (MQ=255)
                                                                                                                                            gACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCg       >  1:2932052/1‑149 (MQ=255)
                                                                                                                                            gACAGATGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCg       >  1:797467/1‑149 (MQ=255)
                                                                                                                                               agatGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTc    >  1:1259495/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GGTGAAACCTCTTCTTATCGTTCAAATCCGATTGGTCTGGCGGAGTTTACCCTGTCTCGCCGCGATCCCGGTTATGTTAGCAGAAGTAAAGCGACTGCTGAGCTGGAAAATCAGCGTCTGGCGGGGAATGTCAGCGAGCTGACAGAGGTGTTTGCGCGCATTAAGCAGATTGCTGGTCAGGAGCATATTGATCCGCTGCAAACTGAAATTGGCAGCATGGTCTATGCGGTGAAACCAGGCGATGGTTCTGCGCGTGAACAGGCGGCGAGCTGCCAGCGTGTGATTGGCGGTCTG  >  CP009273/800633‑800926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑