Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 828,374 G→T 88.3% V11V (GTC→GTA)  ybiA ← DUF1768 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273828,3740GT88.3% 746.9 / 93.0 256V11V (GTC→GTA) ybiADUF1768 family protein
Reads supporting (aligned to +/- strand):  ref base G (18/12);  new base T (105/121);  total (123/133)
Fisher's exact test for biased strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 256 total aligned reads displayed.

TTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGT  >  CP009273/828228‑828519
                                                                                                                                                  |                                                                                                                                                 
    catcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACgtgt                                                                                                                                             <  1:1633893/149‑1 (MQ=255)
     atcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGtt                                                                                                                                            >  2:1682145/1‑149 (MQ=255)
      tcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTg                                                                                                                                           >  2:222087/1‑149 (MQ=255)
      tcaAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTg                                                                                                                                           <  2:1494247/149‑1 (MQ=255)
        aaGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGa                                                                                                                                         >  2:352339/1‑149 (MQ=255)
         aGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGAt                                                                                                                                        >  2:2725493/1‑149 (MQ=255)
           gAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATcc                                                                                                                                      <  2:4119136/149‑1 (MQ=255)
           gAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAAACACCGAAGTCATCGCTGGTGCTGAAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATcc                                                                                                                                      >  1:333027/1‑149 (MQ=255)
             aTTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCtt                                                                                                                                    <  2:4650821/149‑1 (MQ=255)
             aTTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCtt                                                                                                                                    >  2:3015004/1‑149 (MQ=255)
                tttGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTtgt                                                                                                                                 <  1:3954371/149‑1 (MQ=255)
                  tgtgCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGc                                                                                                                               <  1:2940682/149‑1 (MQ=255)
                   gtgCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCt                                                                                                                              >  2:5186284/1‑149 (MQ=255)
                   gtgCCTGAAAAGAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCt                                                                                                                              <  1:3639868/149‑1 (MQ=255)
                    tgCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTc                                                                                                                             <  1:1978715/149‑1 (MQ=255)
                    tgCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTc                                                                                                                             >  2:3371550/1‑149 (MQ=255)
                      ccTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGa                                                                                                                           >  2:3892241/1‑149 (MQ=255)
                      ccTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGa                                                                                                                           >  2:1902656/1‑149 (MQ=255)
                       cTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGaa                                                                                                                          <  1:3176687/149‑1 (MQ=255)
                        tGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAAc                                                                                                                         <  1:5091109/149‑1 (MQ=255)
                          aaaaTAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACgg                                                                                                                       <  1:3110372/149‑1 (MQ=255)
                          aaaaTAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACgg                                                                                                                       >  2:3402020/1‑149 (MQ=255)
                            aaTAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGc                                                                                                                     >  1:5065228/1‑149 (MQ=255)
                                gtgtTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTa                                                                                                                 >  2:1104615/1‑149 (MQ=255)
                                 tgtTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTaa                                                                                                                >  1:890836/1‑149 (MQ=255)
                                 tgtTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTaa                                                                                                                >  1:1183892/1‑149 (MQ=255)
                                  gtTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTaaa                                                                                                               >  1:871229/1‑149 (MQ=255)
                                   ttCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAAc                                                                                                              <  1:4193969/149‑1 (MQ=255)
                                    tCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAAcc                                                                                                             <  2:2307980/149‑1 (MQ=255)
                                         ggTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCAtt                                                                                                        >  2:4126084/1‑149 (MQ=255)
                                          gTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGTATCCTTTGTGCTCGAACGGGCATTAAACCGCATTa                                                                                                       <  2:296984/149‑1 (MQ=255)
                                          gTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTa                                                                                                       >  1:3016133/1‑149 (MQ=255)
                                            aGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCTAAGTTAGAAAAATCTCCGTAGTCATCGCTTGTGATGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATg                                                                                                     <  1:2637515/149‑1 (MQ=255)
                                            aGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATg                                                                                                     <  2:3541062/149‑1 (MQ=255)
                                            aGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATg                                                                                                     <  1:2637663/149‑1 (MQ=255)
                                                  ggTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTtggtg                                                                                               <  1:4873220/149‑1 (MQ=255)
                                                    ttttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTggtggt                                                                                             <  1:2365495/149‑1 (MQ=255)
                                                    ttttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTggtggt                                                                                             <  2:5110504/149‑1 (MQ=255)
                                                    ttgtCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTggtggt                                                                                             <  1:993860/146‑1 (MQ=255)
                                                     tttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGtt                                                                                            <  1:1735124/149‑1 (MQ=255)
                                                      ttCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTa                                                                                           <  1:3393153/149‑1 (MQ=255)
                                                       tCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAt                                                                                          <  2:1229987/149‑1 (MQ=255)
                                                        cccGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAtt                                                                                         <  2:3739521/149‑1 (MQ=255)
                                                        cccGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAtt                                                                                         <  2:3639959/149‑1 (MQ=255)
                                                        cccGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAtt                                                                                         <  2:3343763/149‑1 (MQ=255)
                                                        cccGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTAtt                                                                                         >  2:4917926/1‑149 (MQ=255)
                                                         ccGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTg                                                                                        <  1:1526380/149‑1 (MQ=255)
                                                          cGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGc                                                                                       >  2:3146777/1‑149 (MQ=255)
                                                            tCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGa                                                                                     <  2:94498/149‑1 (MQ=255)
                                                                ccTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCg                                                                                 >  1:2502089/1‑149 (MQ=255)
                                                                ccTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCg                                                                                 >  2:2554898/1‑149 (MQ=255)
                                                                           cAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaa                                                                      >  1:1075069/1‑149 (MQ=255)
                                                                           cAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaa                                                                      >  1:141254/1‑149 (MQ=255)
                                                                           cAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCCTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaa                                                                      <  1:5226844/149‑1 (MQ=255)
                                                                            aGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaac                                                                     <  1:3630075/149‑1 (MQ=255)
                                                                             ggcggcAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCcagaaaca                                                                    >  1:5160753/1‑149 (MQ=255)
                                                                                  cAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGaaaa                                                               >  1:70176/1‑149 (MQ=255)
                                                                                  cAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGaaaa                                                               >  1:4369103/1‑149 (MQ=255)
                                                                                    aaGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAAc                                                             <  2:3288393/149‑1 (MQ=255)
                                                                                      gTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCAAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCa                                                           <  1:3832362/149‑1 (MQ=255)
                                                                                        tAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCAtt                                                         <  2:4503341/149‑1 (MQ=255)
                                                                                         aGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGGGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTa                                                        <  1:2491181/149‑1 (MQ=255)
                                                                                             aaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTAcccc                                                    <  2:3437346/149‑1 (MQ=255)
                                                                                             aaaTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTAcccc                                                    <  2:814126/149‑1 (MQ=255)
                                                                                               aTCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTg                                                  >  2:1309291/1‑149 (MQ=255)
                                                                                                   ccGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGaaaa                                              >  1:4826899/1‑149 (MQ=255)
                                                                                                       aGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGa                                          >  1:3756567/1‑149 (MQ=255)
                                                                                                        gTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaa                                         >  1:4263304/1‑149 (MQ=255)
                                                                                                         tCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaaa                                        >  1:2960648/1‑149 (MQ=255)
                                                                                                         tCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaaa                                        <  2:1982033/149‑1 (MQ=255)
                                                                                                          cATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaaaa                                       >  1:1866303/1‑149 (MQ=255)
                                                                                                           aTCGCTGGTGCTGTAGAATTTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGaaaaa                                      <  2:1857991/149‑1 (MQ=255)
                                                                                                             agCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATg                                    <  2:894937/148‑1 (MQ=255)
                                                                                                              gCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGc                                   >  2:1087205/1‑149 (MQ=255)
                                                                                                               cTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGcc                                  >  2:2126282/1‑149 (MQ=255)
                                                                                                               cTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGcc                                  <  1:3414530/149‑1 (MQ=255)
                                                                                                                tGGTGCTGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCca                                 <  1:3350215/149‑1 (MQ=255)
                                                                                                                      tGTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAatat                           >  2:489290/1‑149 (MQ=255)
                                                                                                                       gTAGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATAtt                          <  1:4949691/149‑1 (MQ=255)
                                                                                                                       gTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATAtt                          >  1:461134/1‑149 (MQ=255)
                                                                                                                         aGAAATTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTgg                        <  2:2607214/149‑1 (MQ=255)
                                                                                                                           aaaTTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCt                      <  1:4784660/149‑1 (MQ=255)
                                                                                                                           aaaTTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCt                      >  2:4855381/1‑149 (MQ=255)
                                                                                                                           aaaTTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCt                      >  2:737654/1‑149 (MQ=255)
                                                                                                                           aaaTTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCt                      <  1:4721163/149‑1 (MQ=255)
                                                                                                                            aaTTTATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTg                     >  2:1840530/1‑149 (MQ=255)
                                                                                                                              tttATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGtt                   <  2:5001092/149‑1 (MQ=255)
                                                                                                                              tttATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGtt                   <  1:3892241/149‑1 (MQ=255)
                                                                                                                              tttATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGtt                   <  1:352339/149‑1 (MQ=255)
                                                                                                                              tttATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGtt                   <  1:2856337/149‑1 (MQ=255)
                                                                                                                                tATGATGGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTta                 <  2:1430485/149‑1 (MQ=255)
                                                                                                                                    atgGTGTCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACa             <  2:5160753/149‑1 (MQ=255)
                                                                                                                                       gtgtCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTa          >  2:648275/1‑149 (MQ=255)
                                                                                                                                        tgtCTTGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTAt         >  1:1303242/1‑149 (MQ=255)
                                                                                                                                          tcgtGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTAttt       <  2:3115401/146‑1 (MQ=255)
                                                                                                                                            ttGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCa     >  1:6577/1‑149 (MQ=255)
                                                                                                                                            ttGCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCa     >  2:1163871/1‑149 (MQ=255)
                                                                                                                                              gCATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAgg   >  2:2447528/1‑149 (MQ=255)
                                                                                                                                               cATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGt  <  1:2669045/149‑1 (MQ=255)
                                                                                                                                               cATTACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGt  <  1:1018707/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGACGTGTTGGATCCTTTGTGCTCGAACGGGCATTAAACCGCATTATGTTGGTGGTTATTGCGAGCCGCTTTCCAGAAACAGAAAAACCATTACCCCTGAAAACCGAAAAATGCCACAATATTGGCTGTTTATACAGTATTTCAGGT  >  CP009273/828228‑828519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑