Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 882,441 A→T 7.3% S119T (TCT→ACT)  ybjJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273882,4410AT 7.3% 1027.4 / 55.1 287S119T (TCT→ACT) ybjJputative transporter
Reads supporting (aligned to +/- strand):  ref base A (114/152);  new base T (10/11);  total (124/163)
Fisher's exact test for biased strand distribution p-value = 8.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 292 total aligned reads displayed.

CCGGAACGCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAA  >  CP009273/882294‑882588
                                                                                                                                                   |                                                                                                                                                   
ccGGAACGCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGaa                                                                                                                                                    <  1:3729305/149‑1 (MQ=255)
ccGGAACGCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGaa                                                                                                                                                    <  2:4215449/149‑1 (MQ=255)
ccGGAACGCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGaa                                                                                                                                                    >  1:913603/1‑149 (MQ=255)
   gAACGCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCa                                                                                                                                                 >  1:4280235/1‑149 (MQ=255)
       gCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAAc                                                                                                                                             <  2:3340289/149‑1 (MQ=255)
        ccAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACt                                                                                                                                            >  2:594075/1‑149 (MQ=255)
          aaaGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTg                                                                                                                                          <  2:4747323/149‑1 (MQ=255)
            aGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGcc                                                                                                                                        <  1:4068171/149‑1 (MQ=255)
            aGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGcc                                                                                                                                        >  2:637129/1‑149 (MQ=255)
              gCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGcccc                                                                                                                                      <  1:4728918/149‑1 (MQ=255)
               ccGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCa                                                                                                                                     <  2:1644405/149‑1 (MQ=255)
                cGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCACAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAAACAAAACTTGCCCCaa                                                                                                                                    <  1:68046/149‑1 (MQ=255)
                   cAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGa                                                                                                                                 <  2:3959209/149‑1 (MQ=255)
                   cAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGa                                                                                                                                 >  2:3612961/1‑149 (MQ=255)
                    aGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGTACCAAAACTTGCCCCAAAGAc                                                                                                                                <  2:2783123/149‑1 (MQ=255)
                    aGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGAc                                                                                                                                >  2:716687/1‑149 (MQ=255)
                     gTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACg                                                                                                                               >  1:1178953/1‑149 (MQ=255)
                      tGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGc                                                                                                                              <  1:2743206/149‑1 (MQ=255)
                      tGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGc                                                                                                                              <  1:3576862/149‑1 (MQ=255)
                        ccATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCg                                                                                                                            <  1:187170/149‑1 (MQ=255)
                          aTCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCgag                                                                                                                          >  1:4916549/1‑149 (MQ=255)
                          aTCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCgag                                                                                                                          >  1:3059675/1‑149 (MQ=255)
                              cGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCa                                                                                                                      <  1:2715904/149‑1 (MQ=255)
                                acacCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAAc                                                                                                                    >  1:1014870/1‑149 (MQ=255)
                                 cacCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACg                                                                                                                   <  1:2115237/149‑1 (MQ=255)
                                  acCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACgg                                                                                                                  >  1:3967743/1‑149 (MQ=255)
                                      gcgcCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCaaa                                                                                                              <  2:2306016/149‑1 (MQ=255)
                                        gcCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAaga                                                                                                            <  2:1980946/149‑1 (MQ=255)
                                         ccTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAagag                                                                                                           <  1:1043330/149‑1 (MQ=255)
                                               aGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAgggg                                                                                                     >  1:1677909/1‑149 (MQ=255)
                                                gCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGc                                                                                                    >  2:1392356/1‑149 (MQ=255)
                                                gCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGc                                                                                                    <  2:1320598/149‑1 (MQ=255)
                                                gCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGc                                                                                                    <  2:1090491/149‑1 (MQ=255)
                                                 cGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCg                                                                                                   <  2:120866/149‑1 (MQ=255)
                                                  gTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGa                                                                                                  <  1:4174321/149‑1 (MQ=255)
                                                   tGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGTACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGAt                                                                                                 >  2:1990993/1‑149 (MQ=255)
                                                   tGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGAt                                                                                                 <  1:4657777/149‑1 (MQ=255)
                                                      ccAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTc                                                                                              >  1:5045481/1‑149 (MQ=255)
                                                        aGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAg                                                                                            >  2:3814869/1‑149 (MQ=255)
                                                          gCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGcc                                                                                          >  2:4580701/1‑149 (MQ=255)
                                                             ataAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGTACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCaga                                                                                       >  1:4303451/1‑149 (MQ=255)
                                                             ataAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCaga                                                                                       >  1:3284064/1‑149 (MQ=255)
                                                               aaaaaCCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGt                                                                                     >  1:3369186/1‑149 (MQ=255)
                                                               aaaaaCCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGt                                                                                     >  1:1809155/1‑149 (MQ=255)
                                                                      gTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGAc                                                                              <  1:2539858/149‑1 (MQ=255)
                                                                        gCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACtt                                                                            <  2:5223947/149‑1 (MQ=255)
                                                                               cGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATatca                                                                     <  2:3716159/149‑1 (MQ=255)
                                                                                 gCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATatcatc                                                                   >  1:593644/1‑149 (MQ=255)
                                                                                 gCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATatcatc                                                                   <  2:4712735/149‑1 (MQ=255)
                                                                                 gCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATatcatc                                                                   <  1:4517255/149‑1 (MQ=255)
                                                                                  cAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATatcatca                                                                  <  2:4488747/149‑1 (MQ=255)
                                                                                  cAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATatcatca                                                                  >  2:4303041/1‑149 (MQ=255)
                                                                                    aaaCCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATatcatcatc                                                                >  1:560250/1‑149 (MQ=255)
                                                                                    aaaCCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATatcatcatc                                                                >  1:4980285/1‑149 (MQ=255)
                                                                                     aaCCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAGGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATcc                                                               >  2:1859857/1‑149 (MQ=255)
                                                                                     aaCCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATcc                                                               <  2:2770969/149‑1 (MQ=255)
                                                                                     aaCCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATcc                                                               <  1:1302331/149‑1 (MQ=255)
                                                                                     aaCCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATcc                                                               <  2:2166512/149‑1 (MQ=255)
                                                                                         gTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGAt                                                           >  1:3185707/1‑149 (MQ=255)
                                                                                          tCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATc                                                          >  2:2373735/1‑149 (MQ=255)
                                                                                            ttATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCaa                                                        <  2:3431217/149‑1 (MQ=255)
                                                                                             tATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAAt                                                       <  2:917108/149‑1 (MQ=255)
                                                                                               ttCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATgc                                                     >  1:3474463/1‑149 (MQ=255)
                                                                                               ttCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATgc                                                     >  1:4893813/1‑149 (MQ=255)
                                                                                                tCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATgcg                                                    >  2:694812/1‑149 (MQ=255)
                                                                                                tCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATgcg                                                    >  1:815199/1‑149 (MQ=255)
                                                                                                tCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATgcg                                                    >  2:783111/1‑149 (MQ=255)
                                                                                                 cATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATgcgc                                                   >  1:4937480/1‑149 (MQ=255)
                                                                                                  aTTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCa                                                  <  2:2971264/149‑1 (MQ=255)
                                                                                                  aTTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCa                                                  <  2:3413755/149‑1 (MQ=255)
                                                                                                   tttCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAg                                                 >  2:4926485/1‑149 (MQ=255)
                                                                                                     tctcGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGa                                               >  2:2892711/1‑149 (MQ=255)
                                                                                                      ctcGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGTACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGAc                                              <  1:3146314/149‑1 (MQ=255)
                                                                                                       tcGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACa                                             <  1:2439351/149‑1 (MQ=255)
                                                                                                        cGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACAt                                            >  1:1396997/1‑149 (MQ=255)
                                                                                                           tCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGt                                         <  2:1462921/149‑1 (MQ=255)
                                                                                                           tCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGt                                         <  2:3894022/149‑1 (MQ=255)
                                                                                                             aaCGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGa                                       <  2:2504501/149‑1 (MQ=255)
                                                                                                               cGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGAcc                                     <  2:3152665/149‑1 (MQ=255)
                                                                                                               cGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGAcc                                     <  2:5057701/149‑1 (MQ=255)
                                                                                                                 gcagcaCCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAg                                   <  1:1516634/149‑1 (MQ=255)
                                                                                                                   agcaCCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGa                                 <  2:4363941/149‑1 (MQ=255)
                                                                                                                   agcaCCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGa                                 <  2:1770843/149‑1 (MQ=255)
                                                                                                                   agcaCCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGa                                 <  1:2075716/149‑1 (MQ=255)
                                                                                                                    gcaCCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGAt                                >  2:1273952/1‑149 (MQ=255)
                                                                                                                    gcaCCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGAACAATGCGCAGGACATCGTGACCAGGAt                                <  2:3885872/149‑1 (MQ=255)
                                                                                                                        cTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACa                            >  1:2240844/1‑149 (MQ=255)
                                                                                                                         ttCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACAt                           >  2:3057205/1‑149 (MQ=255)
                                                                                                                                 ttATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTc                   <  2:278056/149‑1 (MQ=255)
                                                                                                                                  tATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTcc                  <  2:913603/149‑1 (MQ=255)
                                                                                                                                  tATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTcc                  <  1:111531/149‑1 (MQ=255)
                                                                                                                                    tCGCCACTTCCGCAGTACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCa                >  2:1564398/1‑149 (MQ=255)
                                                                                                                                     cGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCaa               >  1:1931561/1‑149 (MQ=255)
                                                                                                                                       ccACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAg             >  2:2736448/1‑149 (MQ=255)
                                                                                                                                         aCTTCCGCAGACCCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCg           >  1:3831563/1‑149 (MQ=255)
                                                                                                                                         aCTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCg           >  1:4817402/1‑149 (MQ=255)
                                                                                                                                           ttCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGtt         >  2:514712/1‑149 (MQ=255)
                                                                                                                                               gCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCGGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCAc     <  1:2846674/149‑1 (MQ=255)
                                                                                                                                                  gAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTaa  >  1:1790938/1‑149 (MQ=255)
                                                                                                                                                  gAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTaa  <  2:4105383/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCGGAACGCCAAAGGCCGTCAGTGCCATCCCGACACCTGCGCCTGCCAGCGTGCCCAGGCTATAAAAACCGTGCATCATCGGCAAAACCGTCTTATTCATTTCTCGCTCAACGGCAGCACCTTCAACGTTTATCGCCACTTCCGCAGAACCAAAACTTGCCCCAAAGACGCCGAGACCAACGGCAAAGAGCAGGGGCGATGTCAGCCAGAGTGCCAGACTTAATATCATCATCCCGATCAATGCGCAGGACATCGTGACCAGGATGACATTACGTGTCCCAAAGCGTTTCACTAA  >  CP009273/882294‑882588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑