Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 924,344 A→T 13.2% L103* (TTG→TAG)  cydC ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273924,3440AT13.2% 859.9 / 105.2 272L103* (TTG→TAG) cydCfused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base A (112/124);  new base T (19/17);  total (131/141)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 291 total aligned reads displayed.

GTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAG  >  CP009273/924197‑924492
                                                                                                                                                   |                                                                                                                                                    
gTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCaa                                                                                                                                                     <  1:4745574/149‑1 (MQ=255)
gTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCaa                                                                                                                                                     <  1:4744939/149‑1 (MQ=255)
 tCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCCATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAAt                                                                                                                                                    >  1:244157/1‑149 (MQ=255)
  caccacCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCTAtt                                                                                                                                                   <  1:758828/149‑1 (MQ=255)
   accaccATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAAttt                                                                                                                                                  <  1:2573767/149‑1 (MQ=255)
      accaTAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCt                                                                                                                                               <  1:1112370/149‑1 (MQ=255)
       ccaTAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTg                                                                                                                                              <  1:1916846/149‑1 (MQ=255)
        caTAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGa                                                                                                                                             >  1:2188849/1‑149 (MQ=255)
               accacAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTa                                                                                                                                      <  2:3856130/149‑1 (MQ=255)
               accacAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTa                                                                                                                                      <  1:3854816/149‑1 (MQ=255)
                 cacacaAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTaaa                                                                                                                                    <  1:3595458/149‑1 (MQ=255)
                 cacaAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATAGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTaaa                                                                                                                                    <  2:3298646/149‑1 (MQ=255)
                      aaGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATgcgc                                                                                                                               >  1:3144946/1‑149 (MQ=255)
                       aGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCa                                                                                                                              <  2:3500893/149‑1 (MQ=255)
                         cgcCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCTATTTGCTGAAGGTGTAAATGCGCAGa                                                                                                                            <  2:4840987/149‑1 (MQ=255)
                          gcCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGAt                                                                                                                           >  2:3439544/1‑149 (MQ=255)
                          gcCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGAt                                                                                                                           >  1:3708436/1‑149 (MQ=255)
                               ccaGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGAtgctgc                                                                                                                      >  1:2039630/1‑149 (MQ=255)
                                caGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCa                                                                                                                     >  1:1037885/1‑149 (MQ=255)
                                    ggcgAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCCATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAacac                                                                                                                 >  1:2333627/1‑149 (MQ=255)
                                    ggcgAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCCATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAacac                                                                                                                 >  1:4956636/1‑149 (MQ=255)
                                      cgAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACAcgc                                                                                                               <  2:78638/149‑1 (MQ=255)
                                          aTAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGaa                                                                                                           >  2:639321/1‑149 (MQ=255)
                                           tAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGaaa                                                                                                          <  2:2779231/149‑1 (MQ=255)
                                           tAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGaaa                                                                                                          <  2:1591143/149‑1 (MQ=255)
                                             aCGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGt                                                                                                        <  1:1431834/149‑1 (MQ=255)
                                              cgcgCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTc                                                                                                       <  1:4381942/149‑1 (MQ=255)
                                                 gcAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCCATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTcgcg                                                                                                    <  1:4874030/149‑1 (MQ=255)
                                                  cAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCTATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGcgt                                                                                                   <  1:1612079/149‑1 (MQ=255)
                                                    ggTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGcgtcg                                                                                                 >  1:2374501/1‑149 (MQ=255)
                                                     gTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCTATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGcgtcgt                                                                                                <  1:1705749/149‑1 (MQ=255)
                                                      tAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTg                                                                                               <  2:2188849/149‑1 (MQ=255)
                                                       aaaGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCCATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGa                                                                                              >  1:709724/1‑149 (MQ=255)
                                                        aaGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCCATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGAc                                                                                             <  2:2046457/149‑1 (MQ=255)
                                                            tgatCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCTATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTAc                                                                                         <  1:4554706/149‑1 (MQ=255)
                                                             gatCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCTATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTAcc                                                                                        >  1:1601721/1‑149 (MQ=255)
                                                             gatCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACTCGGAAAGTCGCGTCGTGACTTAcc                                                                                        <  1:593408/149‑1 (MQ=255)
                                                               tCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAg                                                                                      >  1:3237021/1‑149 (MQ=255)
                                                                  aGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACg                                                                                   >  2:3640727/1‑149 (MQ=255)
                                                                   gCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGt                                                                                  <  1:1767052/149‑1 (MQ=255)
                                                                    cGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGtt                                                                                 <  2:766076/149‑1 (MQ=255)
                                                                      tATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCa                                                                               >  1:1380955/1‑149 (MQ=255)
                                                                        tCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCaaa                                                                             >  2:2338285/1‑149 (MQ=255)
                                                                            cATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAg                                                                         <  2:1415960/149‑1 (MQ=255)
                                                                             aTCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAgc                                                                        <  2:472233/149‑1 (MQ=255)
                                                                              tCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGATCAAAATAgcg                                                                       >  1:391299/1‑149 (MQ=255)
                                                                                  ccaccaCGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCg                                                                   >  2:1095650/1‑149 (MQ=255)
                                                                                  ccaccaCGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCg                                                                   <  2:2784987/149‑1 (MQ=255)
                                                                                  ccaccaCGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCg                                                                   >  2:1796136/1‑149 (MQ=255)
                                                                                      cacGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCTATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAg                                                               >  2:3950492/1‑149 (MQ=255)
                                                                                      cacGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAg                                                               <  1:4679842/149‑1 (MQ=255)
                                                                                        cgcgATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTa                                                             >  2:1150405/1‑149 (MQ=255)
                                                                                         gcgATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCTATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTAc                                                            >  2:1164633/1‑149 (MQ=255)
                                                                                           gATTTAGCAATTCGCCCGGACTATAGCGCGCCAGTTCTGCTGTGTTGAGGGGCAGCTATTTGCTGAAGGTGTAAATGCGCAGATGATGCAACAGGCTTAATGTCGCTTCGTGACTTACCAGACGTTCAAATTAGCGACCGGCAGTTCgt                                                          <  2:5203443/149‑2 (MQ=255)
                                                                                           gATTGAGCCATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACgg                                                          >  2:1684839/1‑149 (MQ=255)
                                                                                           gATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATCGCGCCCGGCAGTACgg                                                          <  1:2737075/149‑1 (MQ=255)
                                                                                             ttGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGt                                                        <  2:2744926/149‑1 (MQ=255)
                                                                                               gAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGa                                                      >  1:2896655/1‑149 (MQ=255)
                                                                                                 gCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGAtt                                                    >  1:4167088/1‑149 (MQ=255)
                                                                                                   aaTTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCTATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATtgc                                                  >  2:4466599/1‑149 (MQ=255)
                                                                                                    aTTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCTATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATtgct                                                 <  2:1003981/149‑1 (MQ=255)
                                                                                                    aTTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCTATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATtgct                                                 <  1:3999365/149‑1 (MQ=255)
                                                                                                    aTTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATtgct                                                 >  1:5127337/1‑149 (MQ=255)
                                                                                                     ttCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATtgctg                                                <  1:2526578/149‑1 (MQ=255)
                                                                                                     gtCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATtgctg                                                <  2:349528/148‑1 (MQ=255)
                                                                                                        gCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGAAGCAATTTGCTGAAGGTTTAAATGCGCAGATGCTGCAACACGCGTAAAGTCGCGTCGTGACTTACCAGACTTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTgcgc                                             <  1:3160870/149‑1 (MQ=255)
                                                                                                           cTGACGATAGCGCGCCAGTCCGGCAGGGTAGTGTGGCAGCAATTTGCTGAAGGTGTAAATGCTCAGATGCTGCAACACGCGGAAAGTCGCGTCTTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCcac                                          <  2:2548975/149‑1 (MQ=255)
                                                                                                            tGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCcacg                                         >  2:5110285/1‑149 (MQ=255)
                                                                                                               cGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCcacgcac                                      <  1:3030689/149‑1 (MQ=255)
                                                                                                                 aTAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCTATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCacgcacgc                                    >  2:3805090/1‑149 (MQ=255)
                                                                                                                 aTAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCacgcacgc                                    >  2:1970648/1‑149 (MQ=255)
                                                                                                                 aTAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCacgcacgc                                    >  2:4584797/1‑149 (MQ=255)
                                                                                                                   aGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGccc                                  <  2:3962966/149‑1 (MQ=255)
                                                                                                                   aGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGccc                                  >  2:434606/1‑149 (MQ=255)
                                                                                                                      gcgcCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCa                               >  2:4250737/1‑149 (MQ=255)
                                                                                                                      gcgcCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCa                               >  2:2206587/1‑149 (MQ=255)
                                                                                                                      gcgcCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCa                               >  2:1532502/1‑149 (MQ=255)
                                                                                                                        gcCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTGACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGc                             <  2:4984245/149‑1 (MQ=255)
                                                                                                                           aGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCggt                          <  2:887424/149‑2 (MQ=255)
                                                                                                                           aGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCAGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCggg                          <  2:1264934/149‑1 (MQ=255)
                                                                                                                            gTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCTTGACTTACCAGACTTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGt                         <  1:786440/149‑1 (MQ=255)
                                                                                                                                ggCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCa                     >  1:3317140/1‑149 (MQ=255)
                                                                                                                                 gCAGGGGAGAGGGGCAGCTATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCat                    >  2:3255423/1‑149 (MQ=255)
                                                                                                                                   aGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCatat                  <  2:2309329/149‑1 (MQ=255)
                                                                                                                                    ggggAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTCGCAtata                 >  2:2689697/1‑149 (MQ=255)
                                                                                                                                    ggggAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCAtata                 >  1:264702/1‑149 (MQ=255)
                                                                                                                                    ggggAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCAtata                 <  2:1908331/149‑1 (MQ=255)
                                                                                                                                     gggAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGAACGCCCGCAGCGGGTAGCATATAg                <  1:1976442/149‑1 (MQ=255)
                                                                                                                                       gagaGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGtt              <  1:1369945/149‑1 (MQ=255)
                                                                                                                                       gagaGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGtt              <  2:1656498/149‑1 (MQ=255)
                                                                                                                                        agagGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTg             <  1:1045658/149‑1 (MQ=255)
                                                                                                                                          agGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGaa           >  1:896804/1‑149 (MQ=255)
                                                                                                                                           ggggCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAg          <  2:2631852/149‑1 (MQ=255)
                                                                                                                                             ggCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCt        <  2:4638895/149‑1 (MQ=255)
                                                                                                                                               cagcaATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGt      >  1:3522942/1‑149 (MQ=255)
                                                                                                                                                 gcaATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTAc    >  1:2760623/1‑149 (MQ=255)
                                                                                                                                                 gcaATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTAc    <  2:3760616/149‑1 (MQ=255)
                                                                                                                                                 gcaATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTATAc    <  2:4122468/149‑1 (MQ=255)
                                                                                                                                                   aaTTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAg  >  2:2504686/1‑149 (MQ=255)
                                                                                                                                                   aaTTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAg  <  2:3158235/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTCACCACCATAATCACCACAAAAGCGCCCACCAGCGGCGAGATAACGCGCAGGTAAAGATGATCGAGCGTATCAACATCCGCCACCACGCGATTGAGCAATTCGCCCTGACGATAGCGCGCCAGTCCGGCAGGGGAGAGGGGCAGCAATTTGCTGAAGGTGTAAATGCGCAGATGCTGCAACACGCGGAAAGTCGCGTCGTGACTTACCAGACGTTCAAAATAGCGCCCGGCAGTACGGGTGATTGCTGCGCCACGCACGCCCGCAGCGGGTAGCATATAGTTGAAGCTGTACAG  >  CP009273/924197‑924492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑