Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,006,218 G→T 9.4% E267* (GAG→TAG)  uup → putative transporter subunit of ABC superfamily: ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,006,2180GT 9.4% 980.2 / 54.3 262E267* (GAG→TAG) uupputative transporter subunit of ABC superfamily: ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base G (131/106);  new base T (19/6);  total (150/112)
Fisher's exact test for biased strand distribution p-value = 5.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 271 total aligned reads displayed.

AATATGGCGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGG  >  CP009273/1006071‑1006366
                                                                                                                                                   |                                                                                                                                                    
aaTATGGCGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAaga                                                                                                                                                     <  1:1203332/149‑1 (MQ=255)
      gCGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGtgtg                                                                                                                                               <  1:2477705/149‑1 (MQ=255)
      gCGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGtgtg                                                                                                                                               <  2:2880581/149‑1 (MQ=255)
       cGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTgg                                                                                                                                              <  1:4821508/149‑1 (MQ=255)
        gACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGa                                                                                                                                             <  2:1236412/149‑1 (MQ=255)
             gcATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGc                                                                                                                                        >  1:1998536/1‑149 (MQ=255)
              cATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGcc                                                                                                                                       <  2:3121073/149‑1 (MQ=255)
                  gttgATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAggg                                                                                                                                   <  2:3333690/149‑1 (MQ=255)
                   ttgATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAgggg                                                                                                                                  <  2:1998159/149‑1 (MQ=255)
                     gATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGTCGCAGGAAGAGGTGTGGATCCGCCAGGGGAt                                                                                                                                <  2:1861460/149‑1 (MQ=255)
                      aTCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATc                                                                                                                               >  1:4588367/1‑149 (MQ=255)
                        ctcGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCaa                                                                                                                             >  2:4049604/1‑149 (MQ=255)
                        ctcGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCaa                                                                                                                             >  2:4307322/1‑149 (MQ=255)
                        ctcGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCaa                                                                                                                             >  2:1507437/1‑149 (MQ=255)
                        ctcGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCaa                                                                                                                             >  2:1689875/1‑149 (MQ=255)
                            aTCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCa                                                                                                                         >  2:3102347/1‑149 (MQ=255)
                               gcgGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGc                                                                                                                      <  1:1065831/149‑1 (MQ=255)
                                 ggCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCg                                                                                                                    >  1:4303991/1‑149 (MQ=255)
                                 ggCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCg                                                                                                                    <  1:2669568/149‑1 (MQ=255)
                                  gCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGt                                                                                                                   >  1:2202890/1‑149 (MQ=255)
                                  gCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGt                                                                                                                   >  2:1340358/1‑149 (MQ=255)
                                  gCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGt                                                                                                                   >  2:4871373/1‑149 (MQ=255)
                                    aaGCTGGTGACCTATCCAGGGAATTACGACCAGGACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTAc                                                                                                                 <  1:1027133/149‑1 (MQ=255)
                                     aGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAATAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTAcc                                                                                                                >  1:2284317/1‑149 (MQ=255)
                                     aGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAATAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTAcc                                                                                                                >  1:1350357/1‑149 (MQ=255)
                                      gCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAATAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTAccc                                                                                                               <  2:769019/149‑1 (MQ=255)
                                      gCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTAccc                                                                                                               <  1:4237391/149‑1 (MQ=255)
                                          gTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTaa                                                                                                           >  1:595653/1‑149 (MQ=255)
                                              ccTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGaa                                                                                                       <  1:36013/149‑1 (MQ=255)
                                              ccTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGaa                                                                                                       >  2:2628749/1‑149 (MQ=255)
                                              ccTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGaa                                                                                                       <  2:17310/149‑1 (MQ=255)
                                                tATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAgg                                                                                                     <  2:3549890/149‑1 (MQ=255)
                                                   ccAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCcg                                                                                                  <  2:2241221/149‑1 (MQ=255)
                                                     aGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAATAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCcgcg                                                                                                >  1:1147700/1‑149 (MQ=255)
                                                     aGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCcgcg                                                                                                >  1:1793696/1‑149 (MQ=255)
                                                      gggAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGt                                                                                               <  2:3894674/149‑1 (MQ=255)
                                                        gAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTAc                                                                                             <  2:4509121/149‑1 (MQ=255)
                                                          aTTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTAcgc                                                                                           >  2:624530/1‑149 (MQ=255)
                                                               gACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCt                                                                                      >  2:4187476/1‑149 (MQ=255)
                                                                aacAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGAAATGAAGGCCGCGTACGCGCCCTg                                                                                     >  2:3942504/3‑149 (MQ=255)
                                                                 ccAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGa                                                                                    >  2:4721569/1‑149 (MQ=255)
                                                                 ccAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGa                                                                                    <  1:201052/149‑1 (MQ=255)
                                                                   aGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGaaa                                                                                  <  2:4604602/149‑1 (MQ=255)
                                                                     tACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGc                                                                                >  1:2859161/1‑149 (MQ=255)
                                                                      aCCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCg                                                                               >  1:4281375/1‑149 (MQ=255)
                                                                        ctgctgGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGAt                                                                             >  1:1584029/1‑149 (MQ=255)
                                                                            tgGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGt                                                                         >  1:5002563/1‑149 (MQ=255)
                                                                                aaaaGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTcgcg                                                                     >  2:408733/1‑149 (MQ=255)
                                                                                aaaaGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTcgcg                                                                     >  1:1681140/1‑149 (MQ=255)
                                                                                  aagaagaagCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGaa                                                                   <  1:2730889/149‑1 (MQ=255)
                                                                                  aagaagaagCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGaa                                                                   <  1:4493970/149‑1 (MQ=255)
                                                                                  aagaagaagCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGaa                                                                   >  2:1125220/1‑149 (MQ=255)
                                                                                   agaagaagCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAATAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAAc                                                                  >  2:300730/1‑149 (MQ=255)
                                                                                   agaagaagCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAAc                                                                  <  2:2015087/149‑1 (MQ=255)
                                                                                   agaagaagCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAAc                                                                  >  2:2604397/1‑149 (MQ=255)
                                                                                     aagaagCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACgt                                                                >  1:4767922/1‑149 (MQ=255)
                                                                                     aagaagCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACgt                                                                <  1:4507426/149‑1 (MQ=255)
                                                                                       gaagCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAATAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACgtgg                                                              >  1:4325642/1‑149 (MQ=255)
                                                                                        aagCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACgtggt                                                             <  1:985984/149‑1 (MQ=255)
                                                                                         agCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAATAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACgtggtg                                                            <  1:3121872/149‑1 (MQ=255)
                                                                                           cccTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGaa                                                          <  2:2340582/149‑1 (MQ=255)
                                                                                            ccTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAAc                                                         >  2:5240615/1‑149 (MQ=255)
                                                                                              tGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAATAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTCATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGt                                                       >  2:2195791/1‑149 (MQ=255)
                                                                                               gcgcGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAATAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTc                                                      >  2:5005639/1‑149 (MQ=255)
                                                                                                    tGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGaa                                                 <  1:1942340/149‑1 (MQ=255)
                                                                                                      gaagaaTTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAATAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGt                                               >  1:3414466/1‑149 (MQ=255)
                                                                                                      gaagaaTTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGt                                               >  1:73392/1‑149 (MQ=255)
                                                                                                      gaagaaTTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGt                                               <  2:3780938/149‑1 (MQ=255)
                                                                                                      gaagaaTTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGt                                               <  1:2459766/149‑1 (MQ=255)
                                                                                                       aagaaTTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTg                                              <  1:570131/149‑1 (MQ=255)
                                                                                                        agaaTTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGa                                             >  2:1441203/1‑149 (MQ=255)
                                                                                                         gaaTTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAATAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAt                                            >  1:2149049/1‑149 (MQ=255)
                                                                                                         gaaTTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGAt                                            <  2:2682092/149‑1 (MQ=255)
                                                                                                           aTTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATgg                                          <  1:330633/149‑1 (MQ=255)
                                                                                                             tACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGt                                        <  1:4043874/149‑1 (MQ=255)
                                                                                                               cAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTAc                                      >  1:4902369/1‑149 (MQ=255)
                                                                                                                    tGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCaa                                 <  2:5229242/149‑1 (MQ=255)
                                                                                                                     gCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCaaa                                <  1:767325/149‑1 (MQ=255)
                                                                                                                     gCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCaaa                                >  1:4435085/1‑149 (MQ=255)
                                                                                                                       cGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGa                              <  2:3240760/149‑1 (MQ=255)
                                                                                                                           ttCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCa                          <  1:5134139/149‑1 (MQ=255)
                                                                                                                             cGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGACCGTCGCGAAGTGATGGGTACCGCAAAGATGCAgg                        >  1:2772550/1‑149 (MQ=255)
                                                                                                                             cGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAgg                        <  2:365560/149‑1 (MQ=255)
                                                                                                                             cGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAgg                        <  2:2220769/149‑1 (MQ=255)
                                                                                                                              gATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGt                       <  1:1466031/149‑1 (MQ=255)
                                                                                                                                tCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTgg                     >  2:1663740/1‑149 (MQ=255)
                                                                                                                                tCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTgg                     >  1:3765185/1‑149 (MQ=255)
                                                                                                                                  gCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGaa                   >  2:3736995/1‑149 (MQ=255)
                                                                                                                                  gCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGaa                   >  1:3286247/1‑149 (MQ=255)
                                                                                                                                   cAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAag                  >  1:3247713/1‑149 (MQ=255)
                                                                                                                                    aaaCTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAaga                 <  1:2407342/149‑1 (MQ=255)
                                                                                                                                     aaCTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAagag                >  1:5070271/1‑149 (MQ=255)
                                                                                                                                       cTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGc              >  1:352941/1‑149 (MQ=255)
                                                                                                                                         ggCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCa            >  1:907496/1‑149 (MQ=255)
                                                                                                                                          gcgcAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCACAGATGCAGGTGGAAGAGGCCAg           >  2:3227716/1‑149 (MQ=255)
                                                                                                                                               ggAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCt      >  2:2127469/1‑149 (MQ=255)
                                                                                                                                                gAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTc     <  2:4975044/149‑1 (MQ=255)
                                                                                                                                                   gagGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGTAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCgg  >  2:3439371/1‑149 (MQ=255)
                                                                                                                                                   gagGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCgg  <  2:3414043/149‑1 (MQ=255)
                                                                                                                                                   gagGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCgg  <  1:420122/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AATATGGCGACGCGCATTGTTGATCTCGATCGCGGCAAGCTGGTGACCTATCCAGGGAATTACGACCAGTACCTGCTGGAAAAAGAAGAAGCCCTGCGCGTGGAAGAATTACAAAATGCCGAGTTCGATCGCAAACTGGCGCAGGAAGAGGTGTGGATCCGCCAGGGGATCAAAGCACGCCGTACCCGTAATGAAGGCCGCGTACGCGCCCTGAAAGCGATGCGTCGCGAACGTGGTGAACGTCGCGAAGTGATGGGTACCGCAAAGATGCAGGTGGAAGAGGCCAGCCGCTCCGG  >  CP009273/1006071‑1006366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑