Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,229,460 G→T 6.1% F238L (TTC→TTA)  nhaB ← sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,229,4600GT 6.1% 1328.8 / 43.8 344F238L (TTC→TTA) nhaBsodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (162/161);  new base T (12/9);  total (174/170)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 358 total aligned reads displayed.

GGCTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACT  >  CP009273/1229312‑1229607
                                                                                                                                                    |                                                                                                                                                   
  cTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGgaagaa                                                                                                                                                   >  1:204025/1‑149 (MQ=255)
   tttGATCGTCAACCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGaaa                                                                                                                                                  <  2:689358/149‑1 (MQ=255)
   tttGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACCTGCGCAGGACGaaa                                                                                                                                                  >  2:306404/1‑149 (MQ=255)
   tttGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGaaa                                                                                                                                                  <  2:4624866/149‑1 (MQ=255)
     tGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATc                                                                                                                                                >  2:5039062/1‑149 (MQ=255)
      gATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCg                                                                                                                                               >  1:1529845/1‑149 (MQ=255)
       aTCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGc                                                                                                                                              >  1:3627190/1‑149 (MQ=255)
         cGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCa                                                                                                                                            <  1:132140/149‑1 (MQ=255)
          gTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCaa                                                                                                                                           >  1:4741415/1‑149 (MQ=255)
          gTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCaa                                                                                                                                           >  2:364293/1‑149 (MQ=255)
            cAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATAGCCaaaa                                                                                                                                         <  1:2775042/149‑1 (MQ=255)
             aaaCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAAt                                                                                                                                        >  1:355213/1‑149 (MQ=255)
             aaaCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAAt                                                                                                                                        >  2:4227400/1‑149 (MQ=255)
               aCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgc                                                                                                                                      >  1:116453/1‑149 (MQ=255)
               aCTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgc                                                                                                                                      >  1:3181571/1‑149 (MQ=255)
                cTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAACTCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgcc                                                                                                                                     <  2:4624984/149‑1 (MQ=255)
                cTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATgcc                                                                                                                                     >  2:3432400/1‑149 (MQ=255)
                cTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATgcc                                                                                                                                     >  2:321537/1‑149 (MQ=255)
                 tGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATgcca                                                                                                                                    <  2:4046094/149‑1 (MQ=255)
                    tGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGCCCTGCGCAGGAAGAAATCGCCAAAATgccagcc                                                                                                                                 >  2:4681263/1‑149 (MQ=255)
                         cacTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAAACAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCg                                                                                                                            >  2:1849377/1‑149 (MQ=255)
                           cTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCt                                                                                                                          >  2:4771094/1‑149 (MQ=255)
                            ttCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCtt                                                                                                                         >  2:4823787/1‑149 (MQ=255)
                            ttCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCtt                                                                                                                         <  2:920190/149‑1 (MQ=255)
                              cgcgGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTa                                                                                                                       <  2:1976502/149‑1 (MQ=255)
                              cgcgGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTa                                                                                                                       <  2:5087262/149‑1 (MQ=255)
                                cgGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGc                                                                                                                     >  2:3924708/1‑149 (MQ=255)
                                cgGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGc                                                                                                                     <  1:4009929/149‑1 (MQ=255)
                                  gACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCga                                                                                                                   <  1:3320450/149‑1 (MQ=255)
                                   aCTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACCGGCCACAAATCAGAACCGGACCGGTCACCGGCGACAGGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCgat                                                                                                                  >  1:648223/1‑149 (MQ=255)
                                   aCTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCgat                                                                                                                  >  2:2938310/1‑149 (MQ=255)
                                      ttCTCCGGCAGTGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCgatgat                                                                                                               <  1:1259187/149‑1 (MQ=255)
                                       tctcCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATc                                                                                                              <  2:2591173/149‑1 (MQ=255)
                                        ctcCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCa                                                                                                             >  1:1410488/1‑149 (MQ=255)
                                         tcCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAg                                                                                                            >  1:137346/1‑149 (MQ=255)
                                         tcCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAg                                                                                                            <  1:1471393/149‑1 (MQ=255)
                                            ggCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGtt                                                                                                         >  2:5227354/1‑149 (MQ=255)
                                            ggCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGtt                                                                                                         <  1:5171579/149‑1 (MQ=255)
                                             gCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTc                                                                                                        <  2:2689843/149‑1 (MQ=255)
                                             gCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTc                                                                                                        <  2:3519186/149‑1 (MQ=255)
                                              cAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAACTCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCt                                                                                                       <  2:1582014/149‑1 (MQ=255)
                                              cAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCt                                                                                                       <  1:2400467/149‑1 (MQ=255)
                                               aGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCtg                                                                                                      >  1:464311/1‑149 (MQ=255)
                                                gCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCtgt                                                                                                     >  1:4865563/1‑149 (MQ=255)
                                                gCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCtgt                                                                                                     >  1:745626/1‑149 (MQ=255)
                                                  gTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTgg                                                                                                   <  1:703224/149‑1 (MQ=255)
                                                    ttCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGtt                                                                                                 <  2:2116945/149‑1 (MQ=255)
                                                     tCACCGTAGCCAAACCACCGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCCGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTc                                                                                                <  2:7441/149‑1 (MQ=255)
                                                               cAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATg                                                                                      <  2:1536023/149‑1 (MQ=255)
                                                                      aCGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCTCCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCTCCATGGTCATTa                                                                               <  2:2332338/149‑1 (MQ=255)
                                                                      aCGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTa                                                                               <  2:1050887/149‑1 (MQ=255)
                                                                        gCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTAcg                                                                             >  2:3594831/1‑149 (MQ=255)
                                                                        gCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTAcg                                                                             >  1:2494096/1‑149 (MQ=255)
                                                                              tctcTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCt                                                                       <  1:456379/149‑1 (MQ=255)
                                                                              tctcTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCt                                                                       >  1:2778854/1‑149 (MQ=255)
                                                                               ctctACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTa                                                                      >  1:5224523/1‑149 (MQ=255)
                                                                               ctctACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTa                                                                      >  1:1706066/1‑149 (MQ=255)
                                                                                tctACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTaa                                                                     <  2:3798199/149‑1 (MQ=255)
                                                                                tctACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTaa                                                                     >  2:4513775/1‑149 (MQ=255)
                                                                                 ctACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAAt                                                                    <  1:1943289/149‑1 (MQ=255)
                                                                                 ctACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAAt                                                                    <  1:5039062/149‑1 (MQ=255)
                                                                                 ctACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAAt                                                                    <  2:3179293/149‑1 (MQ=255)
                                                                                   aCCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGc                                                                  <  2:4577909/149‑1 (MQ=255)
                                                                                   aCCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGc                                                                  <  1:1027457/149‑1 (MQ=255)
                                                                                     cagcagGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCgg                                                                <  2:1800100/149‑1 (MQ=255)
                                                                                      agcagGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAATAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGt                                                               <  2:4394905/149‑1 (MQ=255)
                                                                                        caggcaggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGc                                                             <  1:873876/149‑1 (MQ=255)
                                                                                          ggcaggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCg                                                           <  2:4602779/149‑1 (MQ=255)
                                                                                           gcaggTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGa                                                          <  1:3399578/149‑1 (MQ=255)
                                                                                                  aaCAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGgc                                                   <  1:2956232/149‑1 (MQ=255)
                                                                                                   aCAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGgca                                                  <  2:278228/149‑1 (MQ=255)
                                                                                                   aCAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGgca                                                  <  1:3813494/149‑1 (MQ=255)
                                                                                                   aCAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGgca                                                  <  2:1229702/149‑1 (MQ=255)
                                                                                                       gCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGgcatgca                                              >  2:1993688/1‑149 (MQ=255)
                                                                                                          acaAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGcatca                                           >  1:1640741/1‑149 (MQ=255)
                                                                                                          acaAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGcatca                                           <  1:2002967/149‑1 (MQ=255)
                                                                                                          acaAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGcatca                                           <  1:1254498/149‑1 (MQ=255)
                                                                                                           caAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGcatcat                                          <  2:3340013/149‑1 (MQ=255)
                                                                                                                cAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGc                                     <  2:204025/149‑1 (MQ=255)
                                                                                                                   aaCCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTAc                                  >  2:144062/1‑149 (MQ=255)
                                                                                                                   aaCCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCGTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCCTGGTCATTACGCCGCCTAATGCGGTGCCGACCCCGGCAGGCATCATCAGGCTAc                                  >  1:3953790/1‑149 (MQ=255)
                                                                                                                       ggAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAg                              >  2:425150/1‑149 (MQ=255)
                                                                                                                         aaCGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGaa                            >  2:3855708/1‑149 (MQ=255)
                                                                                                                            ggTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGc                         <  2:3228710/149‑1 (MQ=255)
                                                                                                                              tCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCa                       <  1:1596629/149‑1 (MQ=255)
                                                                                                                                 ccGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACgg                    <  1:4309158/149‑1 (MQ=255)
                                                                                                                                    gCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAAc                 <  1:4139675/149‑1 (MQ=255)
                                                                                                                                    gCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAAc                 <  1:2284643/149‑1 (MQ=255)
                                                                                                                                      gACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTg               >  2:4909660/1‑149 (MQ=255)
                                                                                                                                       aCATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGt              <  1:3697435/149‑1 (MQ=255)
                                                                                                                                       aCATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGt              >  1:3480665/1‑149 (MQ=255)
                                                                                                                                         aTGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTc            >  2:1175654/1‑149 (MQ=255)
                                                                                                                                         aTGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTc            >  1:2731757/1‑149 (MQ=255)
                                                                                                                                          tGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTcc           <  1:4182700/149‑1 (MQ=255)
                                                                                                                                           gcgcAGGAAGAAATCGCCAAACTGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCa          <  1:4543338/149‑1 (MQ=255)
                                                                                                                                             gcAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGa        >  1:1673754/1‑149 (MQ=255)
                                                                                                                                               aGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAAc      >  1:533048/1‑149 (MQ=255)
                                                                                                                                                ggAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAAcc     <  1:2967363/149‑1 (MQ=255)
                                                                                                                                                 gaagaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCa    <  2:2485984/149‑1 (MQ=255)
                                                                                                                                                   agaaATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACt  >  1:2167073/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGCTTTGATCGTCAAACTGTTGCAACACTTCGCGGACTTTCTCCGGCAGCGTTTCACCGTAGCCAAACCAACGCAGCTTCTCTACCAGCAGGCAGGTTAACAGGCCACAAATCAGAACCGGAACGGTCACCGGCGACATGCGCAGGAAGAAATCGCCAAAATGCCAGCCAGCCGCTTTAGCGATGATCAGGTTCTGTGGTTCGCCCACCATGGTCATTACGCCGCCTAATGCGGTGCCGACACCGGCATGCATCATCAGGCTACGCAGAAAGCCACGGAACTGTTCCAGAACCACT  >  CP009273/1229312‑1229607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑