Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,245,461 C→A 8.2% V278V (GTG→GTT)  dhaK ← dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,245,4610CA 8.2% 1057.8 / 52.2 280V278V (GTG→GTT) dhaKdihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (108/149);  new base A (5/18);  total (113/167)
Fisher's exact test for biased strand distribution p-value = 7.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 286 total aligned reads displayed.

AACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGTTTGATCAAGGGAAG  >  CP009273/1245313‑1245609
                                                                                                                                                    |                                                                                                                                                    
aaCCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATc                                                                                                                                                      <  1:4602794/149‑1 (MQ=255)
aaCCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGAGCCAAGATTGTTAACCAGCGCAATc                                                                                                                                                      >  2:4188936/1‑149 (MQ=255)
 aCCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCa                                                                                                                                                     >  2:1116416/1‑149 (MQ=255)
    ggTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCAccc                                                                                                                                                  <  2:4023994/149‑1 (MQ=255)
    ggTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCAccc                                                                                                                                                  <  2:1404514/149‑1 (MQ=255)
    ggTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCAccc                                                                                                                                                  <  2:3187672/149‑1 (MQ=255)
       cATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGAt                                                                                                                                               >  1:2837473/1‑149 (MQ=255)
         tatCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCg                                                                                                                                             >  1:3976721/1‑149 (MQ=255)
          atCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGc                                                                                                                                            >  2:175561/1‑149 (MQ=255)
          atCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGc                                                                                                                                            >  2:2761750/1‑149 (MQ=255)
           tCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATAACCCGATCGcc                                                                                                                                           >  1:2464182/1‑149 (MQ=255)
            ccAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCa                                                                                                                                          >  1:3741774/1‑149 (MQ=255)
            ccAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCa                                                                                                                                          <  2:1654449/149‑1 (MQ=255)
            ccAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCa                                                                                                                                          <  2:3538687/149‑1 (MQ=255)
              aGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGa                                                                                                                                        >  2:3003759/1‑149 (MQ=255)
              aGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGa                                                                                                                                        <  2:4417469/149‑1 (MQ=255)
                 gAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTg                                                                                                                                     <  1:2187852/149‑1 (MQ=255)
                  aGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTgg                                                                                                                                    >  1:2682564/1‑149 (MQ=255)
                  aGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATAACCCGATCGCCAGACTgg                                                                                                                                    >  1:3100746/1‑149 (MQ=255)
                        aGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGgt                                                                                                                              <  2:4968522/149‑1 (MQ=255)
                         gTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGgtt                                                                                                                             <  2:1322644/149‑1 (MQ=255)
                              cgcCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTgtttg                                                                                                                        <  2:655014/149‑1 (MQ=255)
                               gcCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTgtttgg                                                                                                                       >  1:151052/1‑149 (MQ=255)
                               gcCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTgtttgg                                                                                                                       >  1:3620562/1‑149 (MQ=255)
                                ccAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTgtttggt                                                                                                                      >  2:3389176/1‑149 (MQ=255)
                                 cAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTgtttggtt                                                                                                                     <  1:4685978/149‑1 (MQ=255)
                                  aattaaattaCGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTgtttggttt                                                                                                                    <  2:3927214/149‑1 (MQ=255)
                                   attaaattaCGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATAACCCGATCGCCAGACTGGAGCGGTTgtttggtttg                                                                                                                   <  2:2431245/149‑1 (MQ=255)
                                        attaCGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTgttgtt                                                                                                              >  1:4822169/1‑149 (MQ=255)
                                          taCGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGttct                                                                                                            <  1:4341224/149‑1 (MQ=255)
                                             gTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTcc                                                                                                         >  1:1007734/1‑149 (MQ=255)
                                             gTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTcc                                                                                                         >  2:5226020/1‑149 (MQ=255)
                                             gTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTcc                                                                                                         >  2:2091488/1‑149 (MQ=255)
                                                 gATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATAACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGcc                                                                                                     <  1:1042213/149‑1 (MQ=255)
                                                   tAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCaa                                                                                                   >  1:4235450/1‑149 (MQ=255)
                                                    aGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAAc                                                                                                  <  2:2606137/149‑1 (MQ=255)
                                                    aGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAAc                                                                                                  >  1:5172698/1‑149 (MQ=255)
                                                    aGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAAc                                                                                                  <  1:3839728/149‑1 (MQ=255)
                                                     gTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACt                                                                                                 >  1:3706233/1‑149 (MQ=255)
                                                     gTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACt                                                                                                 >  1:1448005/1‑149 (MQ=255)
                                                       cAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGc                                                                                               >  2:592741/1‑149 (MQ=255)
                                                        aaTCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGcc                                                                                              >  2:2025771/1‑149 (MQ=255)
                                                         aTCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCt                                                                                             <  1:4274257/149‑1 (MQ=255)
                                                         aTCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCt                                                                                             <  2:4575299/149‑1 (MQ=255)
                                                         aTCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCt                                                                                             <  2:4809817/149‑1 (MQ=255)
                                                         aTCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCt                                                                                             <  1:3423613/149‑1 (MQ=255)
                                                          tCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGTCTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCtt                                                                                            <  1:4969219/149‑1 (MQ=255)
                                                          tCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCtt                                                                                            <  1:4108253/149‑1 (MQ=255)
                                                           cccGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCttg                                                                                           >  1:764215/1‑149 (MQ=255)
                                                           cccGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCttg                                                                                           <  2:4752018/149‑1 (MQ=255)
                                                           cccGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCttg                                                                                           <  1:3939405/149‑1 (MQ=255)
                                                            ccGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGTAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTTTTCTTCCTGCCAACTGCCttgt                                                                                          >  2:4414189/1‑149 (MQ=255)
                                                            ccGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCttgt                                                                                          <  2:1547136/149‑1 (MQ=255)
                                                             cGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCttgtt                                                                                         >  1:850323/1‑149 (MQ=255)
                                                             cGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCttgtt                                                                                         <  2:3168773/149‑1 (MQ=255)
                                                              gCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCttgttg                                                                                        >  2:3073025/1‑149 (MQ=255)
                                                                   cTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAAt                                                                                   <  1:4478890/149‑1 (MQ=255)
                                                                     ggCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATcc                                                                                 >  1:1396871/1‑149 (MQ=255)
                                                                     ggCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATcc                                                                                 >  1:4448539/1‑149 (MQ=255)
                                                                        aaCGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAg                                                                              >  1:2733950/1‑149 (MQ=255)
                                                                         aCGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGa                                                                             >  1:346235/1‑149 (MQ=255)
                                                                         aCGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGa                                                                             <  2:2474712/149‑1 (MQ=255)
                                                                           gtgtGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGaaa                                                                           <  1:3402090/149‑1 (MQ=255)
                                                                            tgtgGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAAc                                                                          <  1:2247836/149‑1 (MQ=255)
                                                                               ggTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATAACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCa                                                                       <  1:931161/149‑1 (MQ=255)
                                                                                gTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCaa                                                                      <  2:864311/149‑1 (MQ=255)
                                                                                 tCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCaaa                                                                     >  1:4173406/1‑149 (MQ=255)
                                                                                  cAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAg                                                                    <  2:4872297/149‑1 (MQ=255)
                                                                                   aGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGt                                                                   <  1:4510039/149‑1 (MQ=255)
                                                                                        gTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGAt                                                              >  1:654813/1‑149 (MQ=255)
                                                                                         ttATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATg                                                             >  1:186135/1‑149 (MQ=255)
                                                                                          tataGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATgg                                                            <  2:2327491/149‑1 (MQ=255)
                                                                                          tataGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATgg                                                            <  1:1266260/149‑1 (MQ=255)
                                                                                            taGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTa                                                          >  2:380241/1‑149 (MQ=255)
                                                                                             aGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTAt                                                         <  1:2795479/149‑1 (MQ=255)
                                                                                               aCGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATAACCCGATCGCCAGACTGGAGCGGTGGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCATGTTGATAAACCCAGAAACGCAAAGTCCGCTGGGATGa                                                       >  1:985159/1‑149 (MQ=255)
                                                                                                    gTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCAt                                                  >  1:2151760/1‑149 (MQ=255)
                                                                                                     tACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCAtt                                                 <  2:1938087/149‑1 (MQ=255)
                                                                                                     tACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCAtt                                                 <  1:1004227/149‑1 (MQ=255)
                                                                                                      aCAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCAatt                                                <  1:3008415/149‑1 (MQ=255)
                                                                                                         gCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTAcc                                             >  2:618923/1‑149 (MQ=255)
                                                                                                                aaGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAggg                                      <  2:5061373/149‑1 (MQ=255)
                                                                                                                 aGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATAACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGgt                                     >  1:3226226/1‑149 (MQ=255)
                                                                                                                       gTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGaa                               >  2:2796180/1‑149 (MQ=255)
                                                                                                                            gcCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACAttt                          >  1:2334025/1‑149 (MQ=255)
                                                                                                                             ccAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTc                         >  2:1515726/1‑149 (MQ=255)
                                                                                                                             ccAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTc                         >  2:483808/1‑149 (MQ=255)
                                                                                                                              cAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCa                        <  2:2641011/149‑1 (MQ=255)
                                                                                                                                aGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATc                      <  1:2013020/149‑1 (MQ=255)
                                                                                                                                   ttGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGAc                   >  2:3987285/1‑149 (MQ=255)
                                                                                                                                     gTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATTATCCATTTACCAGCAGGGTGTCGAACATTTCATCGACgg                 >  2:4503707/1‑149 (MQ=255)
                                                                                                                                      ttAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGt                >  1:1712513/1‑149 (MQ=255)
                                                                                                                                       tAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGtt               <  1:428075/149‑1 (MQ=255)
                                                                                                                                        aaCCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGttt              >  1:288057/1‑149 (MQ=255)
                                                                                                                                          ccAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGTTTGa            <  2:1011737/149‑1 (MQ=255)
                                                                                                                                          ccAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGTTTGa            <  2:3129758/149‑1 (MQ=255)
                                                                                                                                            aGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGTTTGATc          <  2:5137136/149‑1 (MQ=255)
                                                                                                                                                 aaTCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGTTTGATCAAggg     >  2:176665/1‑149 (MQ=255)
                                                                                                                                                  aTCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTCCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGTTTGATCAAGGGa    <  2:3737351/149‑1 (MQ=255)
                                                                                                                                                   tCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGTTTGATCAAGGGaa   <  1:3066685/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACCGGTCATATCCAGTGAGGTGCAGTACGCGCCAATTAAATTACGTTCGATAGTCAATCCCGCTTGCTGGCAACGTGTGGTCAGGCGGTTATAGACGCCGTACAGCTCAGAAAGCGGAGTTGCGCCAAGATTGTTAACCAGCGCAATCACCCGATCGCCAGACTGGAGCGGTTGTTTGGTTTGTTGTTCTTCCTGCCAACTGCCTTGTTGATAATCCCAGAAACGCAAAGTGCGATGGTATGAGCCATTTACCAGCAGGGTGTCGAACATTTCATCGACGGTTTGATCAAGGGAAG  >  CP009273/1245313‑1245609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑